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研究生:張耿豪
研究生(外文):Keng-Hao Chang
論文名稱:天然璋芝與液態培養璋芝成分之萃取與分析
論文名稱(外文):The components analysis of extract from fruitbody and fermentation broth of Anthodia Camphorata
指導教授:段國仁段國仁引用關係
指導教授(外文):Kow-Jen Duan
學位類別:碩士
校院名稱:大同大學
系所名稱:生物工程學系(所)
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:英文
論文頁數:85
中文關鍵詞:液相層析樟芝
外文關鍵詞:Antrodia CamphorataHPLC
相關次數:
  • 被引用被引用:6
  • 點閱點閱:674
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:3
樟芝是最近非常熱門的一種只生長於台灣的植物,根據最近一連串的研究顯示它有非常高的保健及藥用價值,但是因為其成分非常複雜,一直到現在還無法確定到底它包含多少種成分,又由於其生長不易,所以目前市面上所販售的產品大多是發酵而來,到底其與真正野生的差異如何?再者要如何以HPLC選擇適當的移動相來進行分析,這即是本實驗的目的。
我們根據中華藥典之標準方法進行以70 % 乙醇萃取,又以C18 reverse phase層析管柱進行120分鐘之分析,以求取能儘量完整呈現野生樟芝之層析圖,再針對台灣花蓮、台東、阿里山、等三地野生樟芝進行分析,發現其層析圖譜幾乎一模一樣,相似度非常高。另外我們也嘗試以指紋圖譜波長之方式(210nm、230nm、254nm、280nm)找出最適合分析之波長為254nm,加入的移動相酸源為0.1%的醋酸。在此條件下可分離出最多之成分。
此外野生樟芝乙醇萃取物在波長450nm以下皆有很強之吸收,而人工發酵產品則在400nm以下才有較強的吸收,所以如果要用光譜儀找出簡單之區分方法,此一方法不失為一簡便的區分方法。
Antrodia camphorata is one of Fungus that only found in Taiwan.

People found it contains lots of constituents that can protect liver, and stimulate immune system in recent years.

The study compared the constituents extracted from fruit body and commercial products of Antrodia camphorata. The commercial products were from fermentation broth of Antrodia camphorata.

We tried several parameters to make a finger printing of Antrodia camphorata. Finally, we concluded that the best separation condition was 0.1 % acetic acid gradient with ACN and 254 nm wavelength. The ODS column was used. Under this condition we could obtain the maximum constituents of HPLC spectra.

The constituents of fruit bodies were extracted followed China medicine dictionary. We used 70 % (v/v) ethanol to extract the fruit bodies of Antrodia camphorata from three different places (Taitung, Hualian, and Chiayi). The spectra of the three samples are almost the same. This indicated that only one species of Antrodia camphorata is in Taiwan. It was also found that the constituents extracted from fruit body and fermentation broth were very different. There were very few peaks of spectra from the fermentation broth of Antrodia camphorata.

Ethanol extract of fruit body of Antrodia camphorata has a strong absorption at 450 nm in spectrophotometer. However, the absorption was under 400 nm for the ethanol extract from fermentation broth. By use of spectrophometer, we could differentiate the products of Antrodia camphorata from fruit body or fermentation broth.
Index
Abstact I
Index II
Table index III
Figure index IV
1. Introduction 1
2. Review 2
2.1 Classification of Ganoderma lucidum 2
2.2 Classification of Antrodia Camphorata 5
2.3 Effect of Antrodia Camphorata 5
2.4 The components of Antrodia Camphorata 8
2.5 The culture of Antrodia Camphorata 15
3. Material and Methods 17
3.1 The sourcr of Antrodia Camphorata 17
3.2 Material and Instrument 18
3.3 Analysis parameter 18
3.4 Preparation of sample 19
3.5 System condition 24
3.6 Analysis method 24
3.7 Establish finger print 26
4. Experiment data and result 29
4.1 Analysis by UV/VIS Spectrophotometer 29
4.2 Analysis the Antrodia Camphorata with HPLC44
5. Concussion 72
6. References 74
Wang Bo Che, Chen Qi Zhen. 1994. Ganoderma lucidum, the medical mushroom introduction commonly. P7-10, food industry
Institute for Development Studies, Hsinchu.

Wang Bo Che. 1990. Ganoderma lucidum of medical fungi serial reports (1 ). Food industry 22( 1) : 23- 33.

Shin-Shin Lee. 2003. With | A - ( 1 -3)-D-glucan gathers saacharides and trains promoting immune functions for the index Ganoderma lucidum ferment filtrate. Scientific and technological master's thesis of research institute of food of university of Taiwan.

Sun LuXi , Lu TingZhang. 2001. Setting-up of the antitumor many simple and easy analytical methods of saccharides of Ganoderma lucidum. Personal communication.

Wang XinYi. 2004. Composition analysis of the fermented liquid of deep layer of Antrodia Camphorata. Shih Chien the food nutrition research institute of the university Master's thesis.

Wan-Chan Lee. 2003. The Antrodia Camphorata mycelium the research that is turned into with the active composition of physiology. University of Tunghai Master's thesis of research institute of chemical equation.

I-Hong Lee. 2003. Cultivation of the Antrodia Camphorata mycelium and Polysaccharides body resist the B-mode hepatitis virus' active assessment . Medical thesis for the Doctorate of research institute of institute's China's pharmacy of China.

Chen Si Wei. 2004. With train the influence that the fermented liquid of Antrodia Camphorata produced time grows to the cancer cell.
Scientific and technological master's thesis of research institute of food of university of Taiwan.


Shin-Chang Chang. 2003. Antrodia Camphorata of mycelium assuare liver function assess and hot water exact the body Hydrolysis the influence of the activation on the host metal albumen . Scientific master of research institute of resurgence university food Thesis.

I-Shin Chang. 2002. Ox's Antrodia Camphorata and molecule and living beings appraise systematic research. Agriculture chemistry of university of Taiwan is ground Study carefully master's thesis.

Xiang –Ling Wang. 2004. Regard beans plant as hosts and produce
the Ganoderma lucidum and set aside the ferment liquid and set
aside the influence on allergic response of the ferment result.
Taiwan eats department's master's thesis greatly.

Zhou Yu Xin. 2002. The fingerprint atlas of traditional Chinese
medicine studies technology. Publishing house of chemical
industry.Beijing.

Hui-Ling Chaug, Guei-Rung chao, chin, chu chen, Jeng-Leun Mau. (2001). Non-Volatile taste components of Agaricus blaze, Anthodia camphorate and Cordyceps militaris mycelia. Food chemistry, 74, 203~207.


Yang, J. -H , Lin, H. ----C., & Mau, J. -L. (2001). Non-Volatile taste components of several commercial mushrooms. Food chemistry, 72, 465~471.

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