臺灣博碩士論文加值系統

當二氧化鈦表面受波長低於385nm以下的紫外光照射時會產生強大的氧化能力。在有水和氧存在的條件下，二氧化鈦可藉由紫外線(UV)照射產生高氧化力的氫氧自由基和超氧自由基。這些自由基可攻擊細菌，造成細菌的死亡，因此可防止細菌的污染。但之前文獻所使用的方法，無法很有效評估光觸媒真實的殺菌情形，本實驗採用商業用二氧化鈦塗膜玻片，在紫外線照射下，評估光反應殺菌的效果。以腸球菌(E. faecium)、金黃葡萄球菌(S. aureus) 、大腸菌(E. coli)、 綠膿菌(P. aeruginosa) 、白色念珠菌(C. albicans) 為對象，將這幾種病原菌懸浮於磷酸緩衝生理食鹽水(PBS)中，然後塗佈於二氧化鈦塗膜玻片上，蓋上蓋玻片後，於照射60分鐘期間，細菌存活數的衰減接近一級動力學，我們發現本實驗是創新且提供了評估光觸媒殺菌的好方法。

Titanium dioxide( TiO2) photocatalysts generate free radicals when illuminated by lights having wavelength shorter than 385 nm. These radicals are able to attack bacteria, and may therefore be efficacious in reducing bacterial pollution. In this study, a highly sensitive method for the evaluation of the photoinduced disinfection of a commercial TiO2-contained coating under a ultraviolet(UV) light irradiation was developed. Suspension of Enterococcus faecium, Staphylococcus aureus, Pseudomonas aeruginosa, E.coli, or Candida albicans was put on a surface containing TiO2 and was overlaid with a coverglass. During the UV irradiation for up to 60 min the decay of microbial viability nearly followed the first order kinetics. In the present work, we demonstrate a novel method for the evaluation of bactericidal efficacy of photocatalysts.

ABSTRACT
中文摘要
TABLE OF CONTENTS
LIST OF FIGURES
LIST OF TABLESCHAPTER
I INTRODUCTION
II LITERATURE REVIEW
2.1 Introduction of Photocatalysis
2.2 Structure of Target Microorganisms
2.2.1 Bacteria
2.3 Mechanism of cell killing
2.3.1 The oxidation of coenzyme A (CoA)
2.3.2 The disruption of the cell wall and cell membrane

2.3.3. The damage of DNA and RNA
2.4 TiO2 fixed on glass
2.5 The application of photocatalyst
2.6 Visible light photocatalysis
2.7 TiO2 modification
2.8 Treatment of organic compounds in water using photocatalytic
oxidation
III MATERIALS AND METHODS
3.1 Titanium Dioxide Coating Slide
3.2 Bacteria and Culture Media
3.3 Microbial Culture
3.4 Preparation of Bacteria Suspension
3.5 Photo-inactivation of bacteria under fluorescent light
or ultraviolet (UV) light irradiation
3.6 Time Resolution Of Photoinduced Inactivation Of Bacteria
3.7.Linear Model-First Order Kinetics
3.8.The Integrated Form of a First-Order Kinetics Equation


IV RESULTS AND DISCUSSION

The bactericidal efficacy of TiO2 coating induced by ultraviolet
light
V CONCLUSIONS

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