(3.235.41.241) 您好!臺灣時間:2021/04/11 20:21
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:洪秉良
研究生(外文):Ping-Liang Hung
論文名稱:系統化分析Halobacteriumsp.NRC-1的熱休克蛋白基因
論文名稱(外文):Systematic analysis of Halobacterium sp. NRC-1 heat shock protein genes
指導教授:吳韋訥
指導教授(外文):Wailap Victor Ng
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:醫學生物技術研究所
學門:醫藥衛生學門
學類:醫學技術及檢驗學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:97
中文關鍵詞:熱休克蛋白熱休克反應北方墨點雜交實驗保留性調控序列古生菌
外文關鍵詞:heat shock proteinHalobacterium sp. NRC-1heat shock responseNorthern blot hybridizationconserved sequencevng0208h
相關次數:
  • 被引用被引用:0
  • 點閱點閱:92
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:5
  • 收藏至我的研究室書目清單書目收藏:0
熱休克反應 (heat shock reponse)是細胞為了適應環境改變以促使細胞存活而產生的高度保留性機制。此調控機制在原核生物 (Bacteria)和真核生物 (Eukaryotes)中已有相當多的了解,但在古生菌界 (Archaea)中還不太清楚。在這篇研究中,我們利用Halobacterium sp. NRC-1當作一個系統化模型研究古生菌的熱休克系統。
我們�鬖X電腦分析的方法和北方墨點雜交 (Northern blot hybridization)實驗方式來分析並尋找NRC-1中的熱休克蛋白基因。
我們從NRC-1中已註解為熱休克蛋白基因的上游區域 (upstream sequence)分析出某些具有高度保留性調控序列。利用GCG findpatterns程式尋找NRC-1基因體尋找出具此高度保留性調控序列的基因。我們獲得了104個基因,其中包含已知功能的基因26個、未知功能的conserved hypothetical proteins有20個與hypothetical proteins有58個。
我們利用北方墨點雜交實驗證實hsp1和cctA兩個基因在細胞培養於溫度升高時表現會增加,相反的當細胞培養於相同條件下時lon基因的表現量卻下降約2.5倍。有趣的是三個電腦預測可能新的熱休克蛋白基因vng0208h、vng0906h、vng1297c於升溫培養時其基因的表現程度卻都明顯下降。
這個結果表示了在所有已被註解為熱休克蛋白基因之間並沒有共通的高度保留性的上游序列,並且當溫度上升時這些熱休克蛋白基因表現模式是多變的,這說明了在古生菌界中的熱休克反應機制是一個複雜的系統。因此尚須利用額外的分析來研究調控的機制和此系統的組成。
Heat shock response is a highly conserved mechanism to promote the survival of cells upon environmental change. The regulatory mechanism has been well studied in bacteria and eukaryotes but not in the domain archaea. In this study, Halobacterium sp. NRC-1 is served as a model system to better understand the heat shock system in archaea.
We combined computation analyses and Northern blot hybridization assays to analyze and discover heat shock protein genes in this species. The upstream sequences of the annotated NRC-1 heat shock protein genes were analyzed for conserved motif sequences. Using the GCG findpatterns program to search NRC-1 genome for a putative upstream heat shock regulator sequence, we found 104 HSP candidate genes. Among these are twenty-six genes of known function, twenty conserved hypothetical protein genes, and fifty-eight hypothetical protein genes.
We verified the up regulation of hsp1 and cctA upon culture temperature increases by Northern blot hybridization. On the contrary, the expression of lon decreased by approximately 2.5 times in the same conditions. Interestingly the expression of 3 computation predicted heat shock responsive genes vng0208h, vng0906h, vng1297c were obviously decreased when the cells were cultured at a higher temperature.
The observations of the absence of highly conserved upstream sequence(s) among all of the annotated hsp genes and the variable expression patterns upon temperature shifts suggested heat shock response is a complex system in the domain archaea. Thus the regulatory mechanism and the system components have yet to be discovered by additional analyses.
中文摘要--------------------------------------------------1
英文摘要--------------------------------------------------2
第一章、導論----------------------------------------------3
1.1 Halobacterium sp. NRC-1------------------------------3
1.2 熱休克反應機制---------------------------------------4
1.3 原核生物細菌的熱休克反應機制和調控-------------------5
1.4 真核生物的熱休克反應機制和調控-----------------------6
1.5 古生物的熱休克反應機制-------------------------------9
1.6 研究動機和目的--------------------------------------11

第二章、研究材料與實驗方法-------------------------------12
第一節: 電腦研究分析方法---------------------------------12
2.1.1 尋找Halobacterium sp. NRC-1中被定義的熱休克蛋白----12
2.1.2 確認NRC-1中熱休克蛋白的轉譯起始位置----------------12
2.1.3 比對同源性基因的轉錄調控序列-----------------------13
2.1.4 利用高度保留性的轉錄調控序列尋找新的熱休克蛋白-----14
第二節: 實驗材料與研究方法-------------------------------15
2.2.1: 菌株的培養與操作----------------------------------15
2.2.2: Halobacterium sp. NRC-1 mRNA 的萃取與定量---------17
2.2.3: Halobacterium sp. NRC-1 genomic DNA的萃取與定量---18
2.2.4: 修改質體pNBpA與熱休克蛋白基因的cloning------------20
2.2.5: 北方墨點雜交 (Northern blot hybridization)分析----29
第三章、實驗結果-----------------------------------------36
3.1: NRC-1的熱休克蛋白轉譯起始位置的確認-----------------36
3.2: 搜尋NRC-1熱休克蛋白基因表達的調控序列---------------38
3.3: 預測NRC-1中可能新的熱休克蛋白基因-------------------43
3.4: 質體pNBpA的建構-------------------------------------45
3.5: 改變培養溫度對熱休克蛋白基因表達的影響--------------45
第四章、討論---------------------------------------------51
參考文獻-------------------------------------------------57
表-------------------------------------------------------66
圖-------------------------------------------------------74
Annemieke A. Michels, Bart Kanon, Olivier Bensaude, and Harm H. Kampinga. (1999). Heat Shock Protein (Hsp) 40 Mutants Inhibit Hsp70 in Mammalian Cells. The journal of biological chemistry. 274, 36757–63

Anthony L. Fink. (1999). Chaperone-Mediated Protein Folding. Physiological reviews. 79, 425-449

Axel Mogk and Bernd Bukau. (2004). Molecular Chaperones: Structure of a protein disaggregase. Current Biology. 14, 78–80

Baliga NS, Bonneau R, Facciotti MT, Pan M, Glusman G, Deutsch EW, Shannon P, Chiu Y, Weng RS, Gan RR, Hung P, Date SV, Marcotte E, Hood L, Ng WV. (2004). Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea. Genome Res. 14, 2221-2234

Boy-Marcotte, E., Perrot, M., Bussereau, F., Boucherie, H., and Jacquet, M. (1998). Msn2p and Msn4p control a large number of genes induced at the diauxic transition which are repressed by cyclic AMP in Saccharomyces cerevisiae. J Bacteriol. 180, 1044-1052

Bult CJ, White O, Olsen GJ, Zhou L, Fleischmann RD, Sutton GG, Blake JA, FitzGerald LM, Clayton RA, Gocayne JD, Kerlavage AR, Dougherty BA, Tomb JF, Adams MD, Reich CI, Overbeek R, Kirkness EF, Weinstock KG, Merrick JM, Glodek A, Scott JL, Geoghagen NS, Venter JC. (1996). Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science. 273, 1058-1073

Deppenmeier U, Johann A, Hartsch T, Merkl R, Schmitz RA, Martinez-Arias R, Henne A, Wiezer A, Baumer S, Jacobi C, Bruggemann H, Lienard T, Christmann A, Bomeke M, Steckel S, Bhattacharyya A, Lykidis A, Overbeek R, Klenk HP, Gunsalus RP, Fritz HJ, Gottschalk G. (2002). The genome of Methanosarcina mazei: evidence for lateral gene transfer between bacteria and archaea. J Mol Microbiol Biotechnol. 4, 453-461

Dong Young Kim, Dong Ryoung Kim, Sung Chul Ha, Neratur K. Lokanath, Chang Jun Lee, Hye-Yeon Hwang, and Kyeong Kyu Kim. (2003). Crystal Structure of the protease domain of a Heat-shock Protein HtrA from Thermotoga maritime. The journal of biological chemistry. 278, 6543-6551

Dorothea K. Thompson and Clarles J. Daniles. (1998). Heat shock inducibility of an archaea TATA-like promoter controlled by adjacent sequence element. Molecular.Microbilogy. 27, 541-551

Emmanuelle Boy-Marcotte, Gilles Lagniel, Michel Perrot, Franc�吶ise Bussereau, Anne Boudsocq, Michel Jacquet1 and Jean Labarre. (1999). The heat shock response in yeast: differential regulations and contributions of the Msn2p/Msn4p and Hsf1p regulons. Molecular Microbiology. 33, 274-283

Estruch, F. (2000). Stress-controlled transcription factors, stress-induced genes and stress tolerance in budding yeast. FEMS Microbiol Rev. 24, 469-486.

F. C. Neidhardt, R. A. VanBogelen, and V. Vaughn. (1984). The genetics and regulation of heat-shock proteins. Ann. Rev. Genet. 18, 295-329.

Fitz-Gibbon ST, Ladner H, Kim UJ, Stetter KO, Simon MI, Miller JH. (2002). Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum. Proc Natl Acad Sci U S A. 99, 984-989.

Florence Arsene , Toshifumi Tomoyasu , Bernd Bukau. (2000). The heat shock response of Escherichia coli. International Journal of Food Microbiology. 55, 3–9

Futterer O, Angelov A, Liesegang H, Gottschalk G, Schleper C, Schepers B, Dock C, Antranikian G, Liebl W. (2004). Genome sequence of Picrophilus torridus and its implications for life around pH 0. Proc Natl Acad Sci U S A. 101, 9091-9096

Galagan JE, Nusbaum C, Roy A, Endrizzi MG, Macdonald P, FitzHugh W, Calvo S, Engels R, Smirnov S, Atnoor D, Brown A, Allen N, Naylor J, Stange-Thomann N, DeArellano K, Johnson R, Linton L, McEwan P, McKernan K, Talamas J, Tirrell A, Ye W, Zimmer A, Barber RD, Cann I, Graham DE, Grahame DA, Guss AM, Hedderich R, Ingram-Smith C, Kuettner HC, Krzycki JA, Leigh JA, Li W, Liu J, Mukhopadhyay B, Reeve JN, Smith K, Springer TA, Umayam LA, White O, White RH, Conway de Macario E, Ferry JG, Jarrell KF, Jing H, Macario AJ, Paulsen I, Pritchett M, Sowers KR, Swanson RV, Zinder SH, Lander E, Metcalf WW, Birren B. (2002). The genome of M. acetivorans reveals extensive metabolic and physiological diversity. Genome Res. 12, 532-542

Gross, C.A. (1996). Function and regulation of the heat shock proteins. In: Neidhardt, F.C. (Ed.), Escherichia coli and Salmonella, ASM Press. 1382–1399.

Gudrun Vierke, Afra Engelmann, Carina Hebbeln, and Michael Thomm. (2003). A novel archaeal transcriptional regulator of heat shock response. The journal of biological chemistry. 278, 18–26.

Hendrickson EL, Kaul R, Zhou Y, Bovee D, Chapman P, Chung J, Conway de Macario E, Dodsworth JA, Gillett W, Graham DE, Hackett M, Haydock AK, Kang A, Land ML, Levy R, Lie TJ, Major TA, Moore BC, Porat I, Palmeiri A, Rouse G, Saenphimmachak C, Soll D, Van Dien S, Wang T, Whitman WB, Xia Q, Zhang Y, Larimer FW, Olson MV, Leigh JA. (2004). Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis. J Bacteriol. 186, 6956-6969

Himmelreich,R., Plagens,H., Reniner,B. and Herrmann,R.(1997). Comparative analysis of the genomes of the bacteria Mycoplasma pneumoniae and Mycoplasma genitalium. Nucleic Acids Res. 25, 701-712

Hochstrasser M. (1996). Ubiquitin-dependent protein degradation. Annu Rev Genet. 30, 405-39.

Huynen,M.A and Bork,P.(1998). Measuring genome evolution. Proc. Natl Acad. Sci. USA. 95, 5849-5856

John A Leigh. (1999). Transcriptional regulation in Archaea. Current Opinion in Microbiology. 2, 131-134

Julie A Maupin-Furlow, Malgorzata A Gil, Matthew A Humbard, Phillip Aaron Kirkland, Wei Li, Christopher J Reuter and Amy J Wright. (2005). Archaeal proteasomes and other regulatory proteases. Current Opinion in Microbiology. 8, 720–728

Kalman,S., Mitchell,W., Marathe,R., Lammel,C., Fan,J., Hymann,R.W., Olinger,L., Grimwood,J., Davis,R.W. and Stephens,R.S.(1999). Comparative genomes of Chlamydia pneumoniae and C. trachomatis. Nature Genet. 21, 385-389

Kawarabayasi Y, Hino Y, Horikawa H, Jin-no K, Takahashi M, Sekine M, Baba S, Ankai A, Kosugi H, Hosoyama A, Fukui S, Nagai Y, Nishijima K, Otsuka R, Nakazawa H, Takamiya M, Kato Y, Yoshizawa T, Tanaka T, Kudoh Y, Yamazaki J, Kushida N, Oguchi A, Aoki K, Masuda S, Yanagii M, Nishimura M, Yamagishi A, Oshima T, Kikuchi H. (2001). Complete genome sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain7. DNA Res. 8, 123-140.

Kawarabayasi Y, Hino Y, Horikawa H, Yamazaki S, Haikawa Y, Jin-no K, Takahashi M, Sekine M, Baba S, Ankai A, Kosugi H, Hosoyama A, Fukui S, Nagai Y, Nishijima K, Nakazawa H, Takamiya M, Masuda S, Funahashi T, Tanaka T, Kudoh Y, Yamazaki J, Kushida N, Oguchi A, Kikuchi H, et al. (1999). Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1. DNA Res. 6, 83-101

Kawarabayasi Y, Sawada M, Horikawa H, Haikawa Y, Hino Y, Yamamoto S, Sekine M, Baba S, Kosugi H, Hosoyama A, Nagai Y, Sakai M, Ogura K, Otsuka R, Nakazawa H, Takamiya M, Ohfuku Y, Funahashi T, Tanaka T, Kudoh Y, Yamazaki J, Kushida N, Oguchi A, Aoki K, Kikuchi H. (1998). Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3. DNA Res. 5, 55-76.


Kawashima T, Amano N, Koike H, Makino S, Higuchi S, Kawashima-Ohya Y, Watanabe K, Yamazaki M, Kanehori K, Kawamoto T, Nunoshiba T, Yamamoto Y, Aramaki H, Makino K, Suzuki M. (2000). Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium. Proc Natl Acad Sci U S A. 97, 14257-14262

Keith R. Shockley, Donald E. Ward, Swapnil R. Chhabra, Shannon B. Conners, Clemente I. Montero, and Robert M. Kelly. (2003). Heat Shock Response by the Hyperthermophilic Archaeon Pyrococcus furiosus. Applied and environmental microbiology. 69, 2365–2371.

Klenk HP, Clayton RA, Tomb JF, White O, Nelson KE, Ketchum KA, Dodson RJ, Gwinn M, Hickey EK, Peterson JD, Richardson DL, Kerlavage AR, Graham DE, Kyrpides NC, Fleischmann RD, Quackenbush J, Lee NH, Sutton GG, Gill S, Kirkness EF, Dougherty BA, McKenney K, Adams MD, Loftus B, Peterson S, Reich CI, McNeil LK, Badger JH, Glodek A, Zhou L, Overbeek R, Gocayne JD, Weidman JF, McDonald L, Utterback T, Cotton MD, Spriggs T, Artiach P, Kaine BP, Sykes SM, Sadow PW, D'Andrea KP, Bowman C, Fujii C, Garland SA, Mason TM, Olsen GJ, Fraser CM, Smith HO, Woese CR, Venter JC. (1997). The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus. Nature. 27; 364-370

Lecompte O, Ripp R, Puzos-Barbe V, Duprat S, Heilig R, Dietrich J, Thierry JC, Poch O. (2001). Genome evolution at the genus level: comparison of three complete genomes of hyperthermophilic archaea. Genome Res. 11, 981-993.

Liu T, Lu B, Lee I, Ondrovicova G, Kutejova E, Suzuki CK. (2004). DNA and RNA binding by the mitochondrial lon protease is regulated by nucleotide and protein substrate. J Biol Chem. 279, 13902-13910.

Maeder DL, Weiss RB, Dunn DM, Cherry JL, Gonzalez JM, DiRuggiero J, Robb FT. (1999). Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences. Genetics. 152, 1299-1305.

Marchler, G., Schu» ller, C., Adam, G., and Ruis, H. (1993). A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. EMBO J. 12, 1997-2003

Mayer MP, Bukau B. (1998). Hsp70 chaperone systems: diversity of cellular functions and mechanism of action. Biol Chem. 379, 261-8

M. Patricia Herna´ ndez, Ahmed Chadli, and David O. Toft. (2002). HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor. The journal of biological chemistry. 277, 11873–81.

M. S. Gelfand, E. V. Koonin, A. A. Mironov. (2000). Prediction of transcription regulatory site in Archaea by comparative genomic approach. Nucleic acid research. 28, 695-705

P.A. Lund1, A.T. Large and G. Kapatai. (2003). The chaperonins: perspectives from the Archaea. Biochemical Society Transactions. 31, 681-685

P. Darrell Neufer and Ivor J. Benjamin. (1996). Differential expression of B-crystallin and Hsp27 in skeletal muscle during continuous contractile activity. The journal of biological chemistry. 271, 24089–24095

Pongpan Laksanalamai A Frank T. Robb. (2004). Small heat shock proteins from extremophiles: a review. Extremophiles. 8, 1–11

Ran Rosen and Eliora Z. Ron. (2002). Proteome analysis in the study of the bacterial heat-shock response. Mass Spectrometry Reviews. 21, 244– 265

Ritossa, F. (1962). A new puffing pattern induced by temperature shock and DNP in Dorsophila. Experientia. 18, 571-573

Ross Overbeek, Michael Fonstein, Mark D’Souza, Gordon D. Pusch, and Natalia Maltsev. (1999). The use of gene clusters to infer functional coupling. Proc. Natl. Acad. Sci. 96, 2896-2901.

Ruepp A, Graml W, Santos-Martinez ML, Koretke KK, Volker C, Mewes HW, Frishman D, Stocker S, Lupas AN, Baumeister W. (2000). The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum. Nature. 407, 508-513

Schirmer EC, Glover JR, Singer MA, Lindquist S. (1996). HSP100/Clp proteins: a common mechanism explains diverse functions. Trends Biochem Sci. 21, 289-96.

She Q, Singh RK, Confalonieri F, Zivanovic Y, Allard G, Awayez MJ, Chan-Weiher CC, Clausen IG, Curtis BA, De Moors A, Erauso G, Fletcher C, Gordon PM, Heikamp-de Jong I, Jeffries AC, Kozera CJ, Medina N, Peng X, Thi-Ngoc HP, Redder P, Schenk ME, Theriault C, Tolstrup N, Charlebois RL, Doolittle WF, Duguet M, Gaasterland T, Garrett RA, Ragan MA, Sensen CW, Van der Oost J. (2001). The complete genome of the crenarchaeon Sulfolobus solfataricus P2. Proc Natl Acad Sci U S A. 98, 7835-7840

Slesarev AI, Mezhevaya KV, Makarova KS, Polushin NN, Shcherbinina OV, Shakhova VV, Belova GI, Aravind L, Natale DA, Rogozin IB, Tatusov RL, Wolf YI, Stetter KO, Malykh AG, Koonin EV, Kozyavkin SA. (2002). The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens. Proc Natl Acad Sci U S A. 99, 4644-4649

Smith DR, Doucette-Stamm LA, Deloughery C, Lee H, Dubois J, Aldredge T, Bashirzadeh R, Blakely D, Cook R, Gilbert K, Harrison D, Hoang L, Keagle P, Lumm W, Pothier B, Qiu D, Spadafora R, Vicaire R, Wang Y, Wierzbowski J, Gibson R, Jiwani N, Caruso A, Bush D, Reeve JN, et al. (1997). Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics. J Bacteriol. 179, 7135-7155

Sorger, P.K. (1991). Heat shock factor and the heat shock response. Cell. 65, 363-366


Tatusov,R.L., Mushegian,A.R., Bork,P., Brown,N.P., Hayes, W.S., Borodovsky,M., Rudd,K.E. and Koonin,E.V. (1996). Metabolism and evolution of Haemophilus influenzae deduced from a whole-genome comparison with Escherichia coli. Curr. Biol. 6, 279-291

Teresa A. Phillips, Rujth A. Vanbogelen, Frederick C. Neidhardt. (1984). lon Gene Product of Escherichia coli is a Heat-Shock Protein. Journal of bacteriology. 159, 283-287

Teresa Caldas, Emmanuelle Binet, Philippe Bouloc, Annie Costai, Jean Desgresi, and Gilbert Richarme. (2000). The ftsj/rrmj heat shock protein of escherichia coli is a 23 s ribosomal RNA methyltransferase. The journal of biological chemistry. 275, 16414-16419

Thomas Dandekar, Berend Snel, Martijn Huynen and Peer Bork. (1998). Conservation of gene order: a fingerprint of proteins that physically interact. Trends Biochem. Sci. 23, 324-328

Toshiaki Fukui, Tomohiro Eguchi, Haruyuki Atomi, and Tadayuki Imanaka. (2002). A membrane-bound archaeal lon protease displays ATP-independent proteolytic activity towards unfolded proteins and ATP-dependent activity for folded proteins. Journal of bacteriology. 184, 3689–3698

Wailap Victor Ng, Sean P. Kennedy, Gregory G. Mahairas, Brian Berquist, Min Pan, Hem Dutt Shukla, Stephen R. Lasky, Nitin S. Baliga, Vesteinn Thorsson, Jennifer Sbrogna, Steven Swartzell, Douglas Weir, John Hall, Timothy A. Dahl, Russell Welti, Young Ah Goo, Brent Leithauser, Kim Keller, Randy Cruz, Michael J. Danson, David W. Hough, Deborah G. Maddocks, Peter E. Jablonski, Mark P. Krebs, Christine M. Angevine, Heather Dale, Thomas A. Isenbarger, Ronald F. Peck, Mechthild Pohlschroder, John L. Spudich, Kwang-Hwan Jung, Maqsudul Alam, Tracey Freitas, Shaobin Hou, Charles J. Daniels, Patrick P. Dennis, Arina D. Omer, Holger Ebhardt, Todd M. Lowe, Ping Liang, Monica Riley, Leroy Hood, and Shiladitya DasSarma. (2000). Genome sequence of Halobacterium species NRC-1. PNAS. 97, 12176-12181


Waters E, Hohn MJ, Ahel I, Graham DE, Adams MD, Barnstead M, Beeson KY, Bibbs L, Bolanos R, Keller M, Kretz K, Lin X, Mathur E, Ni J, Podar M, Richardson T, Sutton GG, Simon M, Soll D, Stetter KO, Short JM, Noordewier M. (2003). The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism. Proc Natl Acad Sci U S A. 100, 12984-12988.
Wiederrecht. G., Seto. D., and Parker, C.S. (1988). Isolation of the gene encoding the S. cerevisiae heat shock transcription factor. Cell. 54, 841-853.

Wu, C. (1995). Heat shock transcription factors: structure and regulation. Annu Rev Cell Dev Biol 11, 441-469.

Yen-Ping Kuo, Dorothea K. Thompson, Andrew ST. Jean, Robert L. Charlebois, and Charles J. Daniels. (1997). Characterization of two heat shock genes from Haloferax volcanii: a model system for transcription regulation in the Archaea. Journal of Bacteriology. 179, 6318-6324
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
1. 盧智芳(民93)。科技人才教育滿意度大調查─科技教育的3大危機。Cheers(快樂工作人雜誌),49,84-90。
2. 劉淑慧、林怡青(民91)。國三學生選擇甄選入學學校之抉擇歷程與其影響因素。中華輔導學報,11,71-124。
3. 劉明秋(民78)。生計決策理論與實徵研究之分析。臺南師院學報,22,109-133。
4. 楊淑珍(民87)。生涯阻力檢核表的編製。測驗年刊,45(2),149-172。
5. 張紹勳(民87)。技職院校資管科學生生涯發展、自我統整、工作價值觀、升學就業意願及其相關因素之研究。人力資源學報,10,1-16。
6. 孫仲山(民83)。以「發展」觀點詮釋「職業」概念:舒伯的生涯發展理論評述。技術及職業教育雙月刊,20,25-29。
7. 孫仲山、劉金泉、田福連(民90)。高職學生的職業成熟度。教育研究資訊,9(2),44-57。
8. 陳麗娟(民77)。職業取向的縱貫研究及其相關因素之探討。輔導學報,11,217-263。
9. 黃惠惠(民83)。五專畢業生的初始生涯抉擇與發展。諮商與輔導,107,28-31。
10. 陳麗如(民84)。大學生生涯發展阻隔因素量表: 一個診斷與介入處理的工具。測驗年刊,42,217-226。
11. 康自立(民81)。職業發展理論的探討。職業教育學院學報,1,1-31。
12. 袁志晃(民83)。父母影響中學生職業價值觀念和生涯發展之探討。國立彰化師範大學輔導學報,17,1-31。
13. 林蔚芳(民79)。青少年生涯發展問題與輔導。諮商與輔導,53,33-36。
14. 林清文(民83)。大學生生涯發展問卷編製及大學生生涯決定狀態調查研究。輔導學報,17,175-255
15. 林清文(民82)。生涯決策歷程與生涯不確定概念及其評量之研究。國立僑生大學先修班學報,1,1-23。
 
系統版面圖檔 系統版面圖檔