跳到主要內容

臺灣博碩士論文加值系統

(44.192.79.149) 您好!臺灣時間:2023/06/02 23:39
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:王琇嵐
研究生(外文):Shiu-Lan Wang
論文名稱:純化及分析Phostensin生化特性,一個由KIAA1949所編碼並把一型蛋白質磷酸水解酶標的到F型肌動蛋白的蛋白質
論文名稱(外文):Purification and biochemical characterization of Phostensin, a novel protein phosphatase 1 F-actin cytoskeleton targeting subunit encoded by KIAA1949
指導教授:黃憲斌
指導教授(外文):Hsien-bin Huang
學位類別:碩士
校院名稱:國立中正大學
系所名稱:分子生物研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:105
外文關鍵詞:actin dynamicactin capping proteinF-actinprotein phosphatase 1phostensin
相關次數:
  • 被引用被引用:2
  • 點閱點閱:733
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
一型蛋白質磷酸水解酶(PP1)調節蛋白可以與PP1相結合並把PP1標的到細胞各處。所以PP1可以調控細胞內釵h重要的生物途徑,包括蛋白質合成、醣類代謝、神經傳導和肌肉收縮等。Phostensin (把PP1標的到F-型肌動蛋白細胞骨架的次單元蛋白)是本實驗室所發現的一個新發現的PP1調節蛋白。在本實驗中,本人著重在製備並探討phostensin之生化特性。採用E. coli 蛋白表達系統過量表達thioredoxin-phostensin重組蛋白,並藉由Ni-Sepharose管柱層析法及Blue Sepharose管柱層析法純化之,使純度達98%以上。Phostensin重組蛋白可結合F-型肌動蛋白但不具有促使F-型肌動蛋白成束之活性。Phostensin可減緩受gelsolin結合在barbed end之F-型肌動蛋白之聚合作用,由此可知,phostensin是一個肌動蛋白絲pointed end帽輒J白。除此之外,phostensin可增加gelsolin結合在barbed end之F-型肌動蛋白達穩定態時之臨界濃度。我們的研究指出phostensin可藉由影響肌動蛋白絲之pointed end之聚合及解聚合作用而調節肌動蛋白動力平衡。
Protein phosphatases 1(PP1)controls key biological pathways, including protein synthesis, carbohydrate metabolism, neuron transmission and muscle contraction, via association with a variety of regulatory subunits that target the enzyme to various cellular locations. Phostensin (protein phosphatase 1 F-actin cytoskeleton targeting subunit) is a novel PP1 regulatory protein and is identified by our Lab. In this present, I prepared and characterized the biochemical functions of phostensin. The recombinant thioredoxin-phostensin was over-expressed in E. coli system and purified to more than 98% homogeneity by Ni-Sepharose Chromatography and Blue-Sepharose Chromatography. The recombinant phostensin was bound to F-actin but without actin bundling activity. Phostensin reduced the extent of polymerization when the barbed ends were capped by gelsolin, suggesting that it is a pointed end-capping protein of filaments. In addition, phostensin increased the critical concentration of pointed ends at the steady state when the barbed ends were capped by gelsolin. Our result suggested that phostensin can modulate actin dynamics through affecting the addition and depolymerization of filament pointed ends.
中文摘要‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥i
English abstract‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥ii
目錄‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥iii
圖目錄‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥viii
第一章 緒論‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥1
1-1.1蛋白質激酶與蛋白質磷酸水解酶‥‥‥‥‥‥‥‥‥‥‥‥‥1
1-1.2 發現Protein Phosphatase之緣由‥‥‥‥‥‥‥‥‥‥‥‥‥2
1-2 絲胺酸/酥胺酸蛋白質磷酸水解酶之分類‥‥‥‥‥‥‥‥‥‥3
1-3.1 一型蛋白質磷酸水解酶之分類‥‥‥‥‥‥‥‥‥‥‥‥‥‥4
1-3.2一型蛋白質磷酸水解酶之調控次單位‥‥‥‥‥‥‥‥‥‥‥5
1-4.1 肌動蛋白(Actin)之特性與結構‥‥‥‥‥‥‥‥‥‥‥‥‥9
1-4.2肌動蛋白聚合的動力學(The dynamics of actin assembly)‥‥10
1-4.3 F-型肌動蛋白(+)端增長作用大於(-)端‥‥‥‥‥‥‥‥12
1-4.4 肌動蛋白結合蛋白(Actin binding protein)之分類‥‥‥‥‥13
1-4.4a G-型肌動蛋白結合蛋白(G-actin binding proteins)‥‥‥‥‥13
1-4.4b 帽輒J白(Capping proteins)‥‥‥‥‥‥‥‥‥‥‥‥‥14
1-4.4c 分裂蛋白(Severing porteins)‥‥‥‥‥‥‥‥‥‥‥‥‥15
1-4.4d 肌動蛋白交叉結合蛋白(Actin cross-link protein)‥‥‥‥‥15
1-4.4e細胞膜連結肌動蛋白結合蛋白(Membrane associated actin binding proteins)‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥16
1-5 Phostensin‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥17
第二章 實驗材料與方法‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥20
2-1 實驗材料‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥20
2-1.1 化學藥品‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥20
2-1.2 緩衝溶液之配製‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥20
2-1.3 Nickel- Sepharose column的製備‥‥‥‥‥‥‥‥‥‥‥‥‥23
2-1.4 Blue-Sepharose column的製備‥‥‥‥‥‥‥‥‥‥‥‥‥‥24
2-1.5 12.5% SDS-PAGE的製備‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥24
2-1.6 【32P】phosphorylase a製備‥‥‥‥‥‥‥‥‥‥‥‥‥‥25
2-1.7 G-actin stock製備‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥25
2-1.8 F-actin stock製備‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥26
2-1.9 Pyrene G-actin stock製備‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥26
2-1.10 Pyrene F-actin stock製備‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥26
2-2 實驗方法‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥27
2-2.1 質體pET-32a-phostensin之構築‥‥‥‥‥‥‥‥‥‥‥‥‥27
2-2.2 DNA片段的純化‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥27
2-2.3 接合作用‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥29
2-2.4 勝任細胞 (Competent Cell)之製備‥‥‥‥‥‥‥‥‥‥‥29
2-2.5 轉形作用 (Transformation) ‥‥‥‥‥‥‥‥‥‥‥‥‥‥29
2-2.6 質體DNA 之純化‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥30
2-2.7 重組蛋白之表達‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥30
2-2.8 重組蛋白之純化‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥31
2-2.8a 以Nickel-Sepharose Column純化具有His-Tag之重組蛋白‥31
2-2.8b 以Blue-Sepharose column純化重組蛋白‥‥‥‥‥‥‥‥‥32
2-2.9 透析 (Dialysis) ‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥32
2-2.10 蛋白質磷酸水解酶活性分析(protein phosphatase activity assay)‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥32
2-2.11 F-型肌動蛋白共同沉澱法(actin filaments cosedimentation assay)‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥33
2-2.11a F-型肌動蛋白結合作用分析‥‥‥‥‥‥‥‥‥‥‥‥‥‥33
2-2.11b EGTA處理之F-型肌動蛋白結合作用分析‥‥‥‥‥‥‥33
2-2.11c F-型肌動蛋白束狀活性分析‥‥‥‥‥‥‥‥‥‥‥‥‥‥34
2-2.12 西方印漬法(western blot)‥‥‥‥‥‥‥‥‥‥‥‥‥‥34
2-2.13 螢光標記法(fluorescence assays)‥‥‥‥‥‥‥‥‥‥‥35
2-2.13a F-型肌動蛋白之延長作用(elongation of F-actin)‥‥‥‥35
2-2.13b F-型肌動蛋白之解聚合作用(depolymerization of F-actin)‥36
2-2.13c F-型肌動蛋白(-)端之延長作用‥‥‥‥‥‥‥‥‥‥‥36
2-2.13d F-型肌動蛋白(-)端之解聚合作用‥‥‥‥‥‥‥‥‥‥36
2-2.13e穩定態F-型肌動蛋白(-)端之Cc變化(critical concentration of steady state at pointed end of F-actin)‥‥‥‥‥‥‥‥‥‥‥‥37
第三章 實驗結果‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥38
3-1重組蛋白之製備‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥38
3-1.1 thioredoxin-phostensin重組蛋白之表達‥‥‥‥‥‥‥‥‥‥38
3-1.2 thioredoxin-phostensin重組蛋白之純化‥‥‥‥‥‥‥‥‥‥39
3-1.3 thioredoxin-phostensin 1-129重組蛋白之表達‥‥‥‥‥‥‥40
3-1.4 thioredoxin-phostensin 1-129重組蛋白之純化‥‥‥‥‥‥‥41
3-2 蛋白質磷酸水解酶活性分析法‥‥‥‥‥‥‥‥‥‥‥‥‥‥43
3-3 F-型肌動蛋白共同沉澱法‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥43
3-3.1 證明phostensin可與F-型肌動蛋白交互作用‥‥‥‥‥‥‥44
3-4 F-型肌動蛋白成束活性分析‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥45
3-5 螢光標記法‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥46
3-5.1 F-型肌動蛋白之延長作用‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥46
3-5.2 F-型肌動蛋白之解聚合作用‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥46
3-5.3 F-型肌動蛋白(-)端之延長作用‥‥‥‥‥‥‥‥‥‥‥‥47
3-5.4 F-型肌動蛋白(-)端之解聚合作用‥‥‥‥‥‥‥‥‥‥‥48
3-5.5穩定態F-型肌動蛋白(-)端之Cc變化‥‥‥‥‥‥‥‥‥48
3-5.6 Phostensin 1-129對F-型肌動蛋白(-)端延長作用之影響‥‥49
第四章 討論‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥50
第五章 參考文獻‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥58
附圖‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥71
附錄‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥‥92
1.Handks, S. K., and Hunter, T.(1995)Protein kinase 6. The eukaryotic protein kinase superfamily: kinase(catalytic)domain structure and classification. FASEB J. 9, 576-596
2.Blume-Jensen, P. and Hunter, T.(2001)Oncogenic kinase signaling. Nature 411, 355-365
3.Taylos, S. S., Radzio-Andzelm, E., and Hunter, T.(1995)How do protein kinases discriminate between serine/threonine and tyrosine? Structural insights from the receptor protein-tyrosine kinase. FASEB J. 9, 1255-1266
4.Dhanasekaran N(1998)Cell signaling: an overview. Oncogene 17, 1329-1330
5.Cohen, P.(1989)The structure and regulation of protein phosphatases. Annu. Rev. Biochem. 58, 453-508
6.Shenolinkar, S., and Ingebritsen, T. S.(1984)Protein(serine and threonine)phosphate phosphatases. Methods In Enzymology 107, 102-129
7.Honkanen, R. E., and Golden, T.(2002)Regulators of serine/threonine protein phosphatases at the dawn of a clinical era? Curr. Med. Chem. 9, 2055-2075
8.Shenolinkar, S., and Nairn, A. C.(1991)Protein phosphatases: Recent Progress. In Greengard, P. and Robinson, G.. A.(eds.), Advances in Second Messengers and Phosphoprotein Research. Raven Press, New York, 23, 1-121
9.Griffith, J. P., Kim, J. L., Kim, E. E., Sintchak, M. D., Tomson, J. A. Fitzgibbon, M. J., Fleming, M. A., Caron, P. R., Hsiao, K., and Navia, M. A.(1995)X-ray structure of calcineurin inhibited by the immunophilin-immunosuppressant FKBP12-FK506 complex. Cell 82(3), 507-522
10.Bordelon, J. R., Smith Y., Nairn, A. C., Colbran, R. J., Greengard, P., and Muly, E. C.(2005)Differential localization of protein phosphatase-1? β, and γ1 isoforms in primate prefornal cortex. Cereb. Cortex. Mar 9
11. Lesage, Bart., Beullens, M., Ceulemans, H., Himpens, B., and Bollen, M.(2005)Determinants of the nucleolar targeting of protein phosphatase-1. FEBS Letters 579, 5626-5630
12.Faux, M. C., and Scott, J. D.(1996)More on target with protein phosphorylation: conferring specificity by location. Trends In Biochemical Sciences 21(8)312-315
13.Cohen, P.(2002)Protein phosphatase 1-targeted in many directions. J. Cell. Sci. 115, 241-256
14.Dent, P., Lavoinne, A., Nakieliny, S., Caudwell, F. B., Watt, P., and Choen, P.(1990)The molecular mechanism by which insulin stimulates glycogen synthesis in mammalian skeletal muscle. Nature 348(6299), 302-308
15.Hagiwara, M., Alberts, A., Brindle, P., Meinkoth, J., Feramisco, J., Deng, T., Karin, M., Shenolikar, S., and Montminy, M.(1992)Transcriptional attenuation following cAMP induction requires PP-1-mediated dephosphorylation of CREB. Cell 70, 105-113
16.Facchini, A., Borzı, R. M., and Flamigni, F.(2005)Induction of ornithine decarboxylase in T/C-28a2 chondrocytes by lysophosphatidic acid: Signaling pathway and inhibition of cell proliferation. FEBS Letters 579, 2919-2925
17.Alphonse, G., Cayla, X., Guergnon, J., Dessauge, F., Hospital, V., Rebollo, M. P., Fleischer, A., and Rebollo, A.(2003)Serine/threonine protein phosphatases PP1 and PP2A are key players in apoptosis. Biochimie. 85, 721-726
18.Meijer, L., Pondaven, P., Tung, H. Y. L., Cohen, P., and Wallace, R. W.(1986)Protein phosphorylation and oocyte maturation. II. Inhibition of starfish oocyte maturation by intracellular microinjection of protein phosphatases -1 and -2A and alkaline phosphatase. Exp. Cell Res. 163 489-499
19.Davis, H. P. and Squire, L.(1984)R. Protein synthesis and memory: a review. Psychol. Bull. 96, 518–559
20.Carr, A., Sutliff, R., Weber, C., Allen, P., Greengard, P., Lanerolle, P., Kranias, E., and Paul, R. (2001) Is myosin phosphatase regulated in vivo by inhibitor-1? Evidence from inhibitor-1 knockout mice. J. Physio. L. 534, 357-366
21.Surmeier, D. J., Bargas, J., Hemmings, H. C. J., Nairn, A. C., and Greengard, P.(1995)Modulation of calcium currents by a D1 dopaminergic protein kinase/phosphatase cascade in rat neostriatal neurons. Neuron 14(2), 385-397
22.Greengard, P., Allen, P. B., and Nairn, A. C.(1999)Beyond the dopamine receptor: the DARPP-32/protein phosphatase 1 cascade. Neuron 23(3), 435-447
23.Foulkes, J., and Maller, J. L.(1982)In vivo actions of proein phosphatase inhibitor-2 in Xenopus oocyte. FEBS Letter 150(1), 155-160
24.Huchon, D., Ozon, R., and Demaille, J. G.(1981)Protein phosphatase-1 is involved en Xenopus oocyte maturation. Nature 294(5839), 358-359
25.Cyert, M. S., and Thorner, J.(1989)Putting it on and taking it off: phosphoprotein phosphatase involvement in cell cycle regulation. Cell 57(6), 891-893
26.Kwon, Y. G., Lee, S. Y., Choi, Y., Greengard, P., and Nairn, A. C.(1997)Cell cycle-dependent phosphorylation of mammalian protein phosphatase inhibitor-1 by cdc2 kinase. Proc. Natl. Acad. Sci. USA 94(6), 2168-2173
27.Brandt, H., Lee, E. Y., and Killilea, S. D.(1975)A protein inhibitor of rabbit liver phosphorylase phosphatase. Biochemical And Biophysical Research Communications 63(4), 950-956
28.Aitken, A., Biham, T., and Cohen, P.(1982)Complete primary structure of protein phosphatase inhibitor-1 from rabbit skeletal muscle. E. J. B. 126(2), 235-246
29.Hemmings, H. C., Jr, Greengrad, P., Tung, H. Y., and Cohen, P.(1984)DARPP-32, a dopamine-regulated neuronal phosphoprotein, is a potent inhibitor of protein phosphatase –1. Nature 310, 503-508
30.Nimmo, G. A., and Cohen, P.(1978)The regulation of glycogen metabolism. Purification and characterization of protein phosphatase inhibitor-1 from rabbit skeletal muscle. Eur. J. Biochem. 87(2), 341-351
31.Vandenheede, J. R., Yang, S. D., Merlevede, W., Jurgensen, S., and Chock, P. B.(1985)Kinase FA-mediated regulation of rabbit skeletal muscle protein phosphatase. Reversible phosphorylation of the modulator subunit. J. Biol. Chem. 260, 10512-10516
32.Egloff, M. P., Johnson, D. F., Moorhead, G., Cohen, P. T. W., Cohen, P., and Barford, D.(1997)Structural basis for the recognition of regulatory subunits by the catalytic subunit of protein phosphatase 1. EMBO J. 16, 1876-1887
33.Zhao, S., Lee, E. Y. C.(1997)A protein phosphatase-1-binding motif identified by the panning of a random peptide display library J. Biol. Chem. 272, 28368-28372
34.Kwon, Y. G., Huang, H. B., Desdouits, F., Girault, J. A. Greengrad, P., and Nairn, A. C.(1997)Characterisation of the interaction between DARPP-32 and protein phosphatase 1 (PP-1): DARPP-32 peptides antagonize the interaction of PP-1 with binding proteins. Proc. Natl. Acad. Sci. USA 94, 3536-3541
35.Connor, J. H., Quan, H. N., Ramasawamy, N. T., Zhang, L., Barik, S., Zheng, J., Cannon, J. F., Lee, E. Y. C, and Shenolikar, S.(1998)Inhibitor-1 interaction domain that mediates the inhibition of protein phosphatase 1. J. Biol. Chem. 273, 27716-27724
36.Munro, S., Ceulemans, H., Bollen, M., Diplexcito, J., and Cohen, P. T. W.(2005)A novel glycogen-targeting subunit of protein phosphatase 1 that is regulated by insulin and shows differential tissue distribution in humans and rodents. FEBS Journal 272, 1478-1489
37.Vivo, M., Calogero, R. A., Sansone, F., Calabro, V., Parisi, L., Borrelli, L., Saviozzi, S., and Mantia, G. La.(2001)The human tumor suppressor arf interacts with spinophilin/neurabin II, a type 1 protein-phosphatase-binding protein. J. Biol. Chem. 276, 14161-14169
38.Satoh, A., Nakanishi, H., Obasishi, H., Wada, M., Takahashi, K., Satoh, K. Nishioka, H., Matsuura, Y., Mizoguchi, A., and Takai, Y.(1998)Neurabin-II/spinophilin. An actin filament-binding protein with one pdz domain localized at cadherin-based cell-cell adhesion sites. J. Biol. Chem. 273, 3470-3475
39.Allen, P. B., Ouimet, C. C., and Grengard, P.(1997)Spinophilin, a novel protein phosphatase 1 binding protein localized to dendritic spines. Proc. Natl. Acad. Sci USA 94, 9956-9961
40.Nakanish, H., Obaishi, H., Satoh, A., Wada, M. Mandai, K., Satoh, K., Nishioka, H. Matsuura, Y. Mizoguchi, A., and Takai, Y.(1997)Neurabin: a novel neural tissue-specific actin filament-binding protein involved in neurite formation. J. Cell Biol. 139, 951-961
41.Hervé, J. C., and Sarrouilhe, D.(2006)Protein phosphatase modulation of the intercellular junctional communication: importance in cardiac myocytes. Prog. Biophys. Mol. Biol. 90, 225-248
42.Terry-Lorenzo, R. T., Elliot, E., Weiser, D. C., Prickett, T. D. Brautigan, D. L., Nairn, A. C., and Greengard, P.(2002)Neurabins Recruit Protein Phosphatase-1 and Inhibitor-2 to the Actin Cytoskeleton. J. Biol. Chem. 277, 46535-46543
43.Yan, Z., Hsieh-Wilson, L., Feng, J., Tomizawa, K., Allen, P. B., Fiengerg, A. A., Nairn, A. C., and Greengard, P.(1999)Protein phosphatase 1 modulation of neostriatal AMPA channels: regulation by DARPP-32 and spinophilin. Nat. Neurosci. 2, 13-17
44.Dos remedios,C. G., Chhabra,D., Kekic, M., Dedova, I. V., Tsubakihara, M., Berry, D. A., and Nosworthy, N. J.(2003)Actin Binding Proteins: Regulation of Cytoskeletal Microfilaments. Physiol. Rev. 83, 433-473
45.Kinoslan, H. J., Selden, L.A., Estes J. E., and Gershman, L. C.(1993)Nucleotide binding to actin. Cation dependence of nucleotide dissociation and exchange. J Biol Chem 268, 8683–8691
46.Mermall, V., Post, P.L., and Mooseker, M. S.(1998)Unconventional myosin in cell movement, membrane traffic, and signal transduction. Science 279, 527-533
47.Vale, R. D., and Milligan, R. A.(2000)The way things move: looking under the hood of molecular motor proteins. Science 288, 88-95
48.De, L. A., Cruz, E. M., and Pollard, T. D.(1995)Nucleotide-free actin: stabilization by sucrose and nucleotide binding kinetics. Biochemistry 34: 5452–5461
49.Strzelecka-Golaszwaska, H.(2001)Divalent cations, nucleotides and actin structure. Res. Prob. Cell Differ. 32, 23–42
50.Schutt, C. E., Myslik, J.C., Rozycki, M. D., Gooneskere, N., and Lindverg, U.(1993)The structure of crystalline profilin-beta-actin. Nature 365, 810– 816
51.Cooper, J. A., Giardini, P. A., Soo, F. S., and Theriot, J. A.(2000)Secrets of actin-based motility revealed by a bacterial pathogen. Nature Rev. Mol. Cell Biol. 1, 110-119
52.Kasai, M., Nakano, E., and Oosawa, F.(1965)Polymerization of actin free from nucleotides and divalent cations. Biochim. Biophys. Acta. 94, 494–503
53.Wegner, A., and Isenberg, G.(1983)Twelve fold difference between the critical concentrations of the two ends of actin filaments in physiological salt conditions. Proc. Natl. Acad. Sci. USA 80, 4922–4925
54.Pollard, T. D.(1986)Rate constants for the reactions of ATP- and ADPactin with the ends of actin filaments. J Cell Biol 103, 2747–2754
55.Wegner, A.(1976)Head to tail polymerization of actin. J. Mol. Biol. 108, 139–150
56.Pollard, T. D., and Cooper, J. A.(1986)Actin and actin-binding proteins: a critical evaluation of mechanisms and function. Annu. Rev. Biochem. 55, 987–1035
57.Safer, D., and Nachmeias, V. T.(1994)β Thymosins as actin binding peptides. Bioessays 16, 473–479
58.Didry, D., Carlier, M.F., and Pantaloni, D.(1998)Synergy between actin depolymerizing factor cofilin and profilin in increasing actin filament turnover. J. Biol. Chem. 273, 25602–25611
59.Gremm, D., and Wegner, A.(2000)Gelsolin as a calcium-regulated actin filament-capping protein. Eur. J. Biochem. 267, 4339–4345
60.Sung, L. A., Fan, Y., and Lin C. C.(1996)Gene assignment, expression, and homology of human tropomodulin. Genomics 34, 92–96
61.Pollard, T. D.(1999)Introduction. In: Guidebook to the Cytoskeletal and Motor Proteins, edited by Kreis T and Vale R. Oxford, UK: Oxford Univ. Press, 3–11.
62.Selden, L. A., Kinoslan, H. J., Newman, J., Lincoln, B., Hurwitz, C., Gershman, L. C., and Estes, J.E.(1998)Severing of F-actin by the amino-terminal half of gelsolin suggests internal cooperativity in gelsolin. Biophys. J. 75, 3092–3090
63.Blanchoin, L., Pollard, T. D., and Hitchock-Degregori, S. E.(2001)Inhibition of the Arp2/3 complex-nucleated actin polymerization and branch formation by tropomyosin. Curr. Biol. 11, 1300–1304
64.Mangeat, P. H., and Burridge, K.(1987)Actin-membrane interaction in fibroblasts: what proteins are involved in this association? J. Cell Biol. 99, 95-103
65.Terrak, M., Kerff, F., Langsetmo, K., Tao, T., and Dominguez, R.(2004)Structure basis of protein phosphatase 1 regulation. Nature 429(17), 780-784
66.Campos, M., Fadden, P., Alm, G., Qian, Z., and Haystead, T. A. J. (1996) Identification of protein phosphatase-1-binding proteins by microcystin-biotin affinity chromatography. J. Biol. Chem. 271, 28478-28484.
67.Tu, J., Song, W., and Carlson, M. (1996) Protein phosphatase type 1 interacts with proteins required for meiosis and other cellular processes in Saccharomyces cerevisiae. Mol. Cell. Biol. 16, 4199-4206.
68.Allen, P. B., Quimet, C. C., and Greengard, P. (1997) Spinophilin, a novel protein phosphatase 1 binding protein localized to dendritic spines. Proc. Natl. Acad. Sci. USA 94, 9956-9961.
69.Allen, P.B., Kown, Y. G., Nairn, A. C., and Greengard, P. (1998) Isolation and characterization of PNUTS, a putative protein phosphatase 1 nuclear targeting subunit. J. Biol. Chem. 273, 4089-4095.
70.Moorhead, G. B. G., MacKintosh, C., Morrice, N., and Cohen P (1995) Purification of the hepatic glycogen-associated form of protein phosphatase- 1 by microcystin-Sepharose affinity chromatography. FEBS Lett. 362, 101-105.
71.Tran, H. T., Ulke, A., Morrice, N., Johannes, C. J., and Moorhead, G. B. G. (2004) Proteomic Characterization of Protein Phosphatase Complexes of the Mammalian Nucleus Mol. Cell. Proteomics 3, 257-265.
72.Nagase, T., Kikuno, R., and Ohara, O. (2001) Predication of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins. DNA Res. 8, 319-327.
73.Shigenari, A., Ando, A., Renard, C., Chardon, P., Shiina, T., Kulski, J. K., Yasue, H., and Inoko, H. (2004) Nucleotide sequencing analysis of the swine 433-kb genomic segment located between the non-classical and classical SLA class I gene clusters. Immunogenetics 55, 695-705.
74.Golovanov, A. P., Hautbergue, G. M., Wilson, S. A., and Lian, Lu-Yun.(2004)A simple method for improving protein solubility and long-term stability. J. Am. Chem. Soc. 126, 8933-8939
75.Tsumoto, K., Umetsu, M., Kumagai, I., Ejima, Daisue., Philo, J. S., and Arakawa, T.(2004)Role of arginine in protein refolding, solubilization, and purification. Biotechnol. Prog. 20, 1301-1308
76.Fowler, V. M., Greenfiekd, N. J., and Moyer, J.(2003)Tropomodulin contants two actin filament pointed end-capping domains. J. Biol. Chem. 278, 40000-40009
77.Janmey, P. A., and Matsudairag, P. T.(1988)Functional Comparison of Villin and Gelsolin. J. Biol. Chem. 263, 16738-16743
78.Barron-Casella, E. A., Torres, M. A., Scherer, S. W., Heng, H. H. Q., Tsui, L. C., and Casella, J. F.(1995)Sequence analysis and chromosomal localization of human Cap Z. J. Biol. Chem. 270, 21472-21479
79.Hug, C., Miller, T. M., Torres, M. A., Casella, J. F., and Cooper, J. A.(1992)Identification and characterization of an actin-binding site of CapZ. J. Cell Biol. 116, 923-931
80.Zhai, L., Zhao, P., Panebra, A., Guerrerio, A. L., and Khurana, S.(2001)Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton. J. Biol. Chem. 276, 36163-36167
81.Wagner, L. M., Fowler, V. M., and Takemoto, D. J.(2002)The interaction and phosphorylation of tropomodulin by protein kinase C?in N/N 1003A lens epithelial cells. Mol. Vis. 8, 394-406
82.Gallis, B., Edelman, A. M., Casnellie, J.E., and Krebs, E.G.(1983)Epidermal growth factor stimulates tyrosine phosphorylation of the myosin regulatory light chain from smooth muscle. J. Bio.l Chem. 258, 13089-13093.
83.Werth, D. K., Niedel, J.E., and Pastan, I.(1983)Vinculin, a cytoskeletal substrate of protein kinase C. J. Biol. Chem.258, 11423-11426.
84.Bretscher, A.(1989)Rapid phosphorylation and reorganization of ezrin and spectrin accompany morphological changes induced in A- 431 cells by epidermal growth factor. J. Cell Biol. 108, 921- 930.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top