跳到主要內容

臺灣博碩士論文加值系統

(3.236.84.188) 您好!臺灣時間:2021/07/30 02:56
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:陳文斌
研究生(外文):Wen-Bin Chen
論文名稱:水可溶導電蠶絲/聚苯胺之合成、結構鑑定及其在生物檢測器的應用
論文名稱(外文):Studies on Synthesis, Structure Characterization and Biosensor Application:Water-Soluble and Conducting Silk Fibroin / Polyaniline Composites
指導教授:華沐怡
指導教授(外文):Mu-Yi Hua
學位類別:碩士
校院名稱:長庚大學
系所名稱:化工與材料工程研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:80
中文關鍵詞:蠶絲蛋白聚苯胺水可溶生物檢測器
外文關鍵詞:silk fibrin、polyaniline、water-soluble、biosensor
相關次數:
  • 被引用被引用:1
  • 點閱點閱:263
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
本研究是在蠶絲蛋白中合成出摻雜態且水可溶的共軛導電高分子聚苯胺 ( polyaniline,PAn),並探討蠶絲蛋白/聚苯胺複合物之結構、物性和評估應用至過氧化氫檢測晶片的可行性。
從 FT-IR 和 UV-Vis near IR 中可以看出聚苯胺確實有在蠶絲水溶液中聚合出來;由溶解度、UV-Vis near IR、GPC 和 FE-SEM中可以知道所聚合出來的聚苯胺隨著蠶絲的含量越來越大,所聚合出的聚苯胺顆粒越來越小,甚至在蠶絲/聚苯胺重量比 10/1、15/1 和 20/1 三個條件下,可聚合出具水可溶的摻雜態 PAn;從接觸角可知當樣品經過乙醇處理後,隨著蠶絲含量的增加,蠶絲/聚苯胺複合物會越疏水;從導電度分析和熱重分析可以看出,隨著聚苯胺含量的增加,複合物之導電度和熱穩定性都逐之增加。在生醫檢測器的應用上,比較蠶絲蛋白/聚苯胺重量比15/1複合膜、摻雜態聚苯胺、未摻雜聚苯胺可知,蠶絲蛋白重量比15/1複合膜因為含有水可溶性佳的蠶絲蛋白,分子鏈在水溶液中較膨潤 (swelling),電子最容易通過,而且因為酵素是包埋在蠶絲蛋白/聚苯胺重量比15/1複合膜中,電子所需行經的路徑較短,因此效果會比未摻雜態 PAn 好,所以比摻雜態聚苯胺和未摻雜聚苯胺更適合用於固定化酵素的基材,以作為生醫檢測器材料。
This study was to synthesize water-soluble doped and conjugated conductive polyaniline (PAn) in silk fibron. The structure, physical properties and application as a biosensor of hydrogen peroxide of silk fibron / polyaniline composites were investigated. It was confirmed that polyaniline could be synthesized in silk fibron as measured by Fourier transform Infrared spectroscopy (FT-IR) and ultraviolet –visible-near IR spectrocopy (UV-Vis-near IR). The particle size of polyaniline decreased with the increasing content of silk fibron of the fibron / polyaniline composites. A water-soluble doped and conjugated silk fibron / polyaniline composites was obtained when the weight ratio of silk fibron / polyaniline were 10/1, 15/1, and 20/1 as measured by UV-Vis-near IR, gel permeation chromatography (GPC) and Filed Emittion scanning Electron Micrographs (FE-SEM). When the sample was treated with ethanol, the silk fibron / polyaniline composites became more hydrophibic with the increasing of the content of silk fibron. The conductivity and thermal stability of silk fibron / polyaniline composites increased with increasing the content of polyaniline as analyzed by conductivity and thermo- gravimetric analysis. As for the application of biosensor, the conductivity of three samples of silk fibron / polyaniline with weight ratio of 15/ 1, doped polyaniline and undoped polyaniline were compared. The much higher electron conductivity of silk fibron / polyaniline composite was obtained as compared with the other two samples. The reason was that it contained the good water-soluble silk fibron and the chain of silk fibron was more swelling in water so the electron passed through it was more easily. Moreover, the way of electron pass through was shorter when the enzyme was buried in the film of silk fibron / polyaniline with the weight ratio of 15/ 1, resulting in the better conductivity than that of undoped polyaniline. Therefore, it was concluded that the silk fibron / polyaniline composite with the weight ratio of 15/ 1 was more suitable for the applications as materials of biosensor.
指導教授推薦書...........................................................................................
口試委員會審定書.......................................................................................
授權書.......................................................................................................Ⅲ
誌謝...........................................................................................................Ⅳ
摘要 (中文)............................................................................................. Ⅴ
摘要 (英文)............................................................................................. Ⅵ
目錄...........................................................................................................Ⅷ
圖目錄................................................................................................... VIII
表目錄.................................................................................................. XVI
第一章 緒論...............................................................................................1
1-1 導電高分子簡介.................................................................................1
1-2 聚苯胺簡介.........................................................................................4
1-3 蠶絲.....................................................................................................4
1-4 研究目的.............................................................................................5
第二章 文獻回顧.......................................................................................6
2-1 聚苯胺.................................................................................................6
2-1-1 聚苯胺的合成................................................................................6
2-1-1-1 化學法.......................................................................................6
2-1-1-2 電化學法...................................................................................6
2-1-2 聚苯胺的電化學機構....................................................................6
2-1-3 聚苯胺的結構物性........................................................................8
2-1-4 聚苯胺的改質..............................................................................11
2-2 蠶絲的簡介.......................................................................................17
2-2-1 蠶絲的結構..................................................................................17
2-2-2 蠶絲的化學性質..........................................................................18
2-2-3 蠶絲的物理性質..........................................................................20
2-2-4 蠶絲的紅外線 (IR) 光譜...........................................................20
2-2-5 蠶絲的熱分析..............................................................................21
2-2-6 蠶絲水溶液的改質......................................................................22
2-3 酵素...................................................................................................24
2-3-1 酵素簡介......................................................................................24
2-3-2 酵素的固定化..............................................................................25
2-4 生物檢測器.......................................................................................26
2-4-1 生物檢測器..................................................................................26
2-4-2 聚苯胺在生醫檢測上的應用......................................................28
2-4-3 蠶絲在生醫檢測器上的應用......................................................33
第三章 實驗內容.....................................................................................38
3-1 藥品...................................................................................................38
3-2 實驗合成...........................................................................................39
3-2-1 蠶絲的精練..................................................................................39
3-2-2 蠶絲水溶液的製備......................................................................39
3-2-3 蠶絲和聚苯胺複合物的合成......................................................40
3-3 物性分析...........................................................................................40
3-3-1 物性分析之儀器設備..................................................................40
3-3-2 儀器操作與分析..........................................................................41
3-4 生物檢測器的製備及測量...............................................................43
第四章 實驗結果與討論.........................................................................45
4-1 溶解度 (solubility) ..........................................................................45
4-2 傅立葉紅外線光譜儀 (Fourier Transform Infrared Spectroscopy, FT-IR) 光譜分析..............................................................................46
4-3 紫外光/可見光/近紅外光譜儀 (Ultraviolet-visible near IR spectroscopy, UV-Vis near IR) 光譜分析.........................................49
4-4 凝膠滲透層析儀 (Gel Permeation Chromatography, GPC) 分析..52
4-5 場發射式掃描電子顯微鏡 (Filed Emittion Scanning Electron Micrographs, FE-SEM) 圖...............................................................56
4-6 接觸角分析 (Contact Angle)............................................................58
4-7 導電度測試 (Conductivity)..............................................................60
4-8 熱重分析儀 (Thermogravimetric Analyzer, TGA) 熱重分析.......62
4-9 複合材料在生物檢測器上的應用..................................................65
第五章 結論............................................................................................74
第六章 參考文獻....................................................................................76
1. 陳壽安,“導電高分子:新世代光電材料”,物理雙月刊 (2001)。
2. A. J. Heeger, J. Phys. Chem., B, 105, 8475 (2001)。
3. 林春蕙,華沐怡, “奈米磁性粒子鎳/聚苯胺複合物與鎳/聚乙烯醇複合物之合成、 結構鑑定及其在血栓溶解之應用”,長庚大學 (2004)。
4. 嚴勝裕,華沐怡, “奈米級聚苯胺/鐵磁礦複合物之合成、結構及物性研究 ”,長庚 大學 (2003)。
5. J. H. Huh, E. J. Oh, J. H. Cho, Synt. Met., 137, 965, (2003).
6. Cheremisinoff, P. Nicholas, “Handbook of polymer science and technology, v3”,New York : M. Dekker (1989)
7. 尹良瑩, “中國蠶業史”,華岡 (1980)。
8. F. L. Lu, F. Wudl, M. Nowak, A J. Hegger, J. Am. Chem. Soc., 108, 8311 (1986)
9. S. Stafstrom, J. L. Bredas, A. J. Epstein, H. S. Woo, D. B. Tanner, W. S. Huang, A. G. Macdiarmid, Phys. Rev. Lett., 59, 1464 (1987)
10. J. Tang, X. Jing, B. Wang, Synth. Met., 24, 31 (1988).
11. V. G. Kulkarni, L. D. Campbell, W. R. Mathew, Synth. Met. 30, 321 (1989)
12. R. Murugesan, E. Subraminian, Mat. Chem. Phys., 77, 860 (2002).
13. S. H. Kim, K. W. Oh, T. K. Kim, J. Appl. Polym. Sci., 96, 1035 (2005).
14. W. Pan, S. L. Yang, G. Li, J. M. Jiang, Eur. Polym. J., 41, 2127 (2005).
15. P. Ghosh, S. K. Siddhanta, A. Chakrabarti, Eur. Polym. J., 35, 803 (1999).
16. T. Hion, T. Namiki, N. Kuramoto, Synt. Met., 156, 1327 (2006)
17. R. Gangopadhyay, A. De, G. Ghosh, Symth. Met., 123, 21 (2001).
18. W. Liu, A. Anagnostopoulosa, F. F. Bruno, K. Senecal, J. Kumar, S. Tripathy, L. Samuelsonb, Synth. Met., 101, 738 (1999)
19. M. R. Nabid, R. Sedghi, P. R. Jamaat, N. Safari, A. A. Entazami, J. Appl. Polym. Sci., 102, 2929 (2006)
20. Y. H. Geng, J. Li, Z. C. Sun, X. B. Jing, X. H. Wang, F. S. Wang, Polymer, 40, 5723 (1999)
21. S. A. Chen, G. W. Hwang, J. Am. Chem. Soc. 117, 10055 (1995)
22. S. A. Chen, G. W. Hwang, Macro. 29, 3950 (1996)
23. 張之單,“野蠶學”,國光圖書 (1979)。
24. 陳運造,“認識家蠶”,台灣省立博物館 (1991)。
25. 林獻龍,“蠶絲的精練和改質加工”,染化雜誌 (1997)。
26. 邵建中、鄭今歡、劉今強,“蠶絲絲素表面結構與活性染料染色( 二 )”,紡織學報 (2002)。
27. H. Yoshomozu, T. Asakura, J. App. Polym. Sci., 40, 1745 (1990).
28. M. Tsukada, J. Seric. Sc. Jpn., 55, 126 (1986).
29. M. Tsukada, Y. Ishiguro, J. Seric. Sc. Jpn., 53, 121 (1984).
30. T. Arai, D. L. Wilson, N. Kasai, S. Hayasaka, M. Tsukada, J. Appl. Polym. Sci., 84, 1963 (2002).
31. Q. Peng, Q. Xu, H. Xu, M. Pang, J. Li, D. Sun, J. Appl. Polym. Sci., 98, 864, (2005).
32. L. Dai, J. Li, E. Yamada, J. Appl. Polym. Sci., 86, 2342 (2002).
33. H. Y. Kweon, I. C. Um, Y. H. Park, Polymer, 42, 6651 (2001).
34. C. H. Du, B. K. Zhu, J. Y. Chen, Y. Y. Xu, Polym. Int., 55, 377 (2006).
35. G.Yang, L. Zhang, X. Cao, Y. Liu, J. Mem. Sci., 210, 379 (2002).
36. E. Marsano, M. Canetti, G. Conio, P. Corsini, G. Freddi, J. Appl. Polym. Sci., 104, 2187, (2007).
37. K. E. Park, S. Y. Jung, S. J. Lee, B. M. Min, W. H. Park, Inter. J. Biological Macro., 38, 165, (2006).
38. H. J. Jin, J. Park, R. Valluzzi, P. Cebe, D. L. Kaplan, Biomacromolecules, 5, 711, (2004).
39. 劉英俊,汪金追,“酵素工程” (1987)。
40. 陳國城,“酵素工程學” (1989)。
41. Y. Q. Zhang, Bio. Adv., 16, 961 (1998).
42. Y. Q. Zhang, W. D. Shen, R. A. Gu, J. Zhu, R. Y. Xue, Anal. Chim. Acta, 369, 123 (1998).
43. J. Qian, Y. Liu, H. Liu, T. Yu, J. Deng, Anal. Bio., 236, 208 (1996).
44. 呂峰洲、林仁混,“基礎酵素學” (1991)。
45. G. R. M. Ntlatseng, M. Aoife, I. I. Emmanuel, Talanta, 64, 115 (2004).
46. W. J. Cho, H. J. Huang, Anal. Chem., 70, 3946 (1998).
47. P. Andrea, S. Miroslav, S. Silvia, M. Stainslav, Talanta, 54, 763 (2001).
48. S. Hu, J. Luo, D. Cui, Ana. Sci., 15, 585 (1999).
49. J. J. Langer, M. Filipiak, J. Kecinska, J. Jasnoaska, J. Wlodarczak, B. Buladowski, Surface Sc., 573, 140 (2004).
50. X. Pan, J. Kan, L. Yuan, Sensors and Actuators B, Chemical, 102, 325 (2004).
51. A. A. Karyakin, O. A. Bobrova, L. V. Lukachova, E. E. Karyakina, Sensors and Actuators B, Chemical, 33, 34 (1996).
52. K. Grennan, A. J. Killard, M. R. Smyth, Electroanalysis, 17, 1360 (2005).
53. D. D. Borole, U. R. Kapadi, P. P. Mahulikar, D. G. Hundiwale, Polym. Adv. Tech., 15, 306 (2004).
54. H. Xue, Z. Shen, H. Zheng, J. Appl. Elect., 32, 1265 (2002).
55. Y. Q. Zhang, Bio. Adv., 16, 961 (1998).
56. H. Liu, J. Qian, Y. Liu, T. Yu, J. Deng, Biocatalysis and Biotran., 39, 303 (1996).
57. Y. Liu, X. Zhang, H. Liu, T. Yu, J. Deng, j. Biochem., 46, 131 (1996).
58. Y. Liu, J. Qian, X. Fu, H. Liu, J. Deng, Enz. Micro. Tech., 21, 154 (1997).
59. Y. Liu, J. Qian, H. Liu, X. Zhang J. Deng, J. Appl. Polym. Sci., 61, 641 (1996).
60. H. Liu, Z. Zhang, X. Zhang, D. Qi, Electro. Acta, 42, 349 (1997).
61. Y. Liu, H. Liu, J. Qian, J. Deng, T. Yu, Anal. Chim. Acta. 316, 65 (1995).
62. Y. Liu, J. Qian, X. Fu, H. Liu, J. Deng, T. Yu, Enz. Mico. Tech., 21, 154 (1997).
63. H. Liu, Z. Zhang, X. Zhang, D. Qi, Y. Liu, T. Yu, J. Deng, Electro. Acta, 42, 349 (1997).
64. H. Liu, J. Qian, Y. Liu, T. Yu, J. Deng, Bioelectro. Bioenerg., 39, 303, (1996).
65. Y. Liu, H. Liu, J. Qian, J. Deng, T. Yu, Anal. Chim. Acta, 316, 65 (1995).
66. J. Qian, Y. Liu, H. Liu, T. Yu, J. Deng, J. Electroanal. Chem. Interfacial Electrochem., 397, 157 (1995).
67. Y. Liu, J. Qian, H. Liu, X. Zhang, J. Deng, J. Appl. Polym. Sci., 61, 641 (1996).
68. J. Qian, Y. Liu, H. Liu, T. Yu, J. Deng, Anal. Bio., 236, 208 (1996).
69. Y. Q. Zhang, W. D. Shen, R. A. Gu, J. Zhu, R. Y. Xue, Anal. Chem. Acta, 369, 123 (1998).
70. Y. F. Tu, Z. Q. Fu, H. Y. Chen, Sensors and Actuators B, Chemical, 80, 101 (2001).
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top