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研究生:林佳蓉
研究生(外文):Chia jung Lin
論文名稱:探討陰道鞭毛蟲ADF(actin-depolymerizingfactor)/Cofilin在纖維連接蛋白黏附作用之調控機轉
論文名稱(外文):Regulation of Fibronectin-Mediated Adherence through ADF (actin-depolymerizing factor)/ Cofilinin Trichomonas vaginalis
指導教授:鄧致剛鄧致剛引用關係
指導教授(外文):Petrus Tang
學位類別:碩士
校院名稱:長庚大學
系所名稱:基礎醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:93
中文關鍵詞:陰道鞭毛蟲ADF (actin-depolymerizing factor)/ Cofilin纖維連接蛋白黏附作用
外文關鍵詞:Trichomonas vaginalisADF (actin-depolymerizing factor)/
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陰道鞭毛蟲症是一種非病毒性由性接觸所傳染的疾病。當陰道鞭毛蟲寄生在陰道時,陰道鞭毛蟲會藉由黏附作用吸附在陰道上皮細胞,引起細胞病變。纖維連接蛋白為組成陰道壁細胞外基質的重要成份,故可將陰道鞭毛蟲培養於含纖維連接蛋白的環境以模擬陰道鞭毛蟲對陰道表皮細胞的黏附作用。研究結果發現,培養液的pH值和細胞密度會影響陰道鞭毛蟲對纖維連接蛋白的黏附作用。在許多物種中,ADF (actin-depolymerizing factor)/Cofilin已被證實為調控細胞骨架的主要因子,我們在陰道鞭毛蟲表達序列標籤資料庫中發現兩個序列(Tv-ADF-1和Tv-ADF-2)具ADF/Cofilin相似序列,經the Institute for Genomic Research Microbial Database (TIGR)陰道鞭毛蟲基因資料庫比對,發現具有ADF基因特有的signature及domain的open reading frame;以SWISS MODEL模擬三級結構,發現此ORF具有肌動蛋白結合位。利用Real-Time PCR作基因表達定量分析,發現在阿米巴狀的陰道鞭毛蟲中,ADF表現增加;而其他調控細胞骨架相關基因actin-related protein (arp) 2/3 complex、gelsolin、fimbrin和coronin表現增加,formin、kinesin、capping protein和14-3-3 protein A isoform表現下降。
Trichomoniasis is a nonviral sexually transmitted disease. Trichomonas vaginalis cytoadheres to vaginal epithelial cells (VEC) and causes contact-dependent cytotoxicity which leads to erosion of the epithelium. The morphology of T. vaginalis transformed from ellipsoid to an amoeboid form when attached to the vaginal epithelial cells. Previous studies showed that fibronectin genes, the major extracellular matrix component of VEC were overexpressed in T. vaginalis attached cells. We investigated the effects of cell density and pH on cytoadherence of T. vaginalis culture on fibronectin (FN). We showed that pH 7 is the optimum pH for cytoadherence. ADF/Cofilin is a key regulator of cytoskeleton in many organisms. Two Trichomonas ADF/Cofilin homologues were identified and characterized in the present study. The putative proteins contain classical signature /domain of ADF and an actin binding site. Tv ADF was up-regulated in fibronectin-induced amoeboid form at pH 7. Cytoskeletal genes such as actin-related protein (arp) 2/3 complex, gelsolin, fimbrin and coronin were also up-regulated but formin, capping protein, kinesin, profilin and 14-3-3 protein A isoform not.
指導教授推薦書…………………………………………………………
口試委員會審定書………………………………………………………
電子檔案上網授權書…………………………………………………iii
著作授權書……………………………………………………………iv
誌謝……………………………………………………………………v
中文摘要………………………………………………………………vi
英文摘要……………………………………………………………vii
目錄………………………………………………………………viii
圖表目錄…………………………………………………………xi
第一章 緒論……………………………………………………………1
第一節 前言……………………………………………………………1
第二節 陰道鞭毛蟲……………………………………………………1
2.1 外部形態…………………………………………………………1
2.2 超微結構…………………………………………………………2
2.3 生理特性…………………………………………………………3
2.4 生活史與傳染途徑………………………………………………3
2.5 診斷與治療………………………………………………………4
2.6 黏附作用…………………………………………………………4
第三節 纖維連接蛋白 (Fibronectin, FN) ……………………………5
第四節 Cofilin/ADF (actin-depolymerizing factor) …………6
4.1 簡介………………………………………………………………6
4.2 ADF/Cofilin的調控機制………………………………………7
4.3 ADF/Cofilin在生物中扮演的角色……………………………9
第二章、材料與方法……………………………………………………12
第一節 細胞培養………………………………………………………12
第二節 化學試劑………………………………………………………12
第三節 緩衝液和溶液…………………………………………………12
第四節 蟲體的計算與觀察……………………………………………12
第五節 核酸引子………………………………………………………13
第六節 聚合酶鏈反應 (Polymerase chain reaction, PCR)……………13
第七節 連鎖聚合酶反應產物純化……………………………………13
第八節 核酸洋菜膠電泳分析…………………………………………13
第九節 DNA的萃取與純化...…………………………………………14
9.1 陰道鞭毛蟲基因體DNA的萃取……………………………………14
9.2 大腸桿菌質體DNA的萃取…………………………………………14
第十節 陰道鞭毛蟲RNA的萃取…………………………………………15
第十一節 北方墨點法……………………………………………………15
11.1探針製作………………………………………………………………15
11.2甲醛洋菜膠電泳分析法 (Formamide gel electrophoresis)…15
11.3 RNA轉印至轉漬膜………………………………………………………15
11.4 北方雜交法………………………………………………………………16
第十二節 重組蛋白質的篩選與純化……………………………………………16
第十三節 西方墨點法……………………………………………………………16
13.1陰道鞭毛蟲蛋白質的萃取…………………………………………………16
13.2重組蛋白質…………………………………………………………………17
13.3蛋白質定量…………………………………………………………………17
13.4 聚丙醯胺凝膠 (Sodium dodecyl sulphate-polyacrylamide gel)配
製……………………………………………………………………………………17
13.5 胺凝膠電泳…………………………………………………………………17
13.6 蛋白質轉漬(Electrotransfer)..……………………………………18
13.7 抗體反應及偵測……………………………………………………………18
第十四節 細胞黏著試驗 (Cytoadherence assay)…………………………18
第十五節 DNAase處理……………………………………………………………18
第十六節 Reverse transcriptase PCR(cDNA synthesis)……………19
第十七節 Real Time PCR……………………………………19
第十八節 銀染色 (Silver stain)…………………………19
第十九節 質譜 (Mass spectrometry , MS)測定………20
19.1 In gel digestion……………………………………20
19.2 基質輔助雷射脫附游離/飛行時間質普分析儀分析……20
第二十節 抗體的製備……………………………………………21
第三章、結果.....................................22
第四章、討論.....................................30
第五章、結論.....................................39
參考文獻........................................40
附錄…………………………………………………………………52
圖……………………………………………………………………52
表……………………………………………………………………89
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