跳到主要內容

臺灣博碩士論文加值系統

(35.172.136.29) 您好!臺灣時間:2021/07/26 21:21
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:曾詩涵
研究生(外文):Shr-Han Tzeng
論文名稱:探討陰道鞭毛蟲ADP核糖基化因子與貼附的關係
論文名稱(外文):The role of ADP-ribosylation factor on fibronectin mediated adherence in Trichomonas vaginalis
指導教授:鄧致剛鄧致剛引用關係
指導教授(外文):Petrus Tang
學位類別:碩士
校院名稱:長庚大學
系所名稱:基礎醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:87
中文關鍵詞:腺嘌呤核苷二磷酸核醣化因子陰道鞭毛蟲
外文關鍵詞:ADP-ribosylation factor (Arf)Trichomonas vaginalis
相關次數:
  • 被引用被引用:0
  • 點閱點閱:69
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
在真核細胞中,當蛋白質由其合成的地方形成時,會藉由細胞內的運輸系統把具有不同功能的蛋白質送到特定的胞器中。而ADP核糖化因子(ADP-ribosylation factors, Arfs)屬於高度保守性的小GTP水解酶家族,它調控並影響生物膜的形成與細胞內物質的傳送。
根據陰道鞭毛蟲表現序列標幟資料庫中的資訊顯示:當蟲體貼附於纖維連接蛋白(fibronectin)時,tv_arf1a mRNA的表達量遠高於其它tv_arf異構物;而陰道鞭毛蟲貼附於纖維連接蛋白並形成阿米巴形體,主要可模擬人類陰道感染蟲體的狀況。然而至目前為止,關於ADP核糖化因子在陰道鞭毛蟲中所扮演的角色仍有許多未知存在。
我們在陰道鞭毛蟲的體內找到一個ADP核糖化因子,這個tv_arf1a的序列與人類的arf具有高度同源性,它含有181個胺基酸,並與哺乳動物的第一類型arf具極高的相似性,同時也包含一個Arf功能區塊(domain),這個區塊可當作Arf家族的辨識序列;利用北方墨點法得知tv_arf1a mRNA長度約為630個鹼基,而依同步定量聚合酶連鎖反應的結果中獲知,在滋養體以及阿米巴型體時,tv_arf1a的表達量都高於其它的tv_arf異構物;同時我們也成功獲得一個多株抗體可以偵測到陰道鞭毛蟲體內的約21 kDa大小的Tv_Arf1a蛋白質。
When proteins are synthesized within eukaryotic cells, they will be delivered to the specific organelles according to their different functions by the intracellular protein transport systems. ADP-ribosylation factors (Arfs) belong to a highly conserved family of small GTPases that are responsible for membrane dynamics and trafficking.
The Trichomonas vaginalis Expressed Sequence Tag (EST) database revealed that tv_arf1a mRNA is expressed at a higher level than other tv_arf isoforms in the firbonectin-mediated cytoadherence EST library. Fibronectin-mediated adherence is essential for the parasite to establish infection in the human vagina. However, relatively little is known about the role of Arf in the human parasite, T. vaginalis.
We have isolated the first arf gene from T. vaginalis. The sequence of the tv_arf1a is homologus to human arf genes. The gene encodes a putative protein of 181 amino acids with high similarity to the mammalian class I Arf proteins and contained the Arf domain that designated it as an Arf family member. Northern analysis identified a single tv_arf1a mRNA of approximately 630 bases in the trophozoite form of the parasite. Real-time PCR showed that tv_arf1a was expressed at a higher level than other tv_arf isoforms in both the trophozoite and amoeboid forms. A polyclonal antibody recognized the Tv_Arf1a with a predicted molecular mass of 21 kDa in T. vaginalis.
 目 錄

§ 指導教授推薦書
§ 口試委員會審定書
§ 授權書───────────────────────III
§ 誌謝────────────────────────V
§ 中文摘要──────────────────────VI
§ 英文摘要──────────────────────VII
§ 目錄────────────────────────VIII

§ 第一章 研究背景資料─────────────────1
1.1陰道鞭毛蟲的簡介──────────────────1
1. 1. 2 陰道鞭毛蟲的外部型態與超顯微構造────────2
1. 1. 3 陰道鞭毛蟲的生活史與生理特性──────────3
1. 1. 4 傳染途徑與流行病學───────────────4
1. 2 綜觀腺嘌呤核苷二磷酸核醣化因子───────────5
1. 2. 1 Arf蛋白質家族的成員───────────────7
1. 2. 2 Arfs的循環︰在活化態與非活化態間的循環機制與位置轉移─8
1. 2. 3 Arfs在細胞中所扮演的角色────────────9
1. 2. 4 Arfs協助COPI囊泡的形成─────────────10
1. 2. 5 囊泡在高基氏體與內質網間的運輸─────────11
1. 3 研究動機──────────────────────12

§ 第二章 實驗材料與方法─────────────────14
2.1 細胞培養──────────────────────14
2.2 試劑與藥品─────────────────────14
2.3 緩衝液與溶液────────────────────14
2.4 DNA片段電泳分析──────────────────15
2.5 陰道鞭毛蟲Genomic DNA的萃取────────────15
2.6 寡聚核苷酸引子的合成────────────────16
2.7 聚合酶連鎖反應(Polymerase Chain Reaction, PCR) ──17
2.8 陰道鞭毛蟲總量RNA的萃取────────────────18
2.9 甲醛膠體電泳(Formaldehyde Gel Electrophoresis) ──18
2.10 探針的製備───────────────────────19
2.11 北方墨點法(Northern Blotting) ───────────19
2.12 反轉錄聚合酶連鎖反應(Reverse Transcription PCR, RT-PCR)─20
2.13 同步定量聚合酶連鎖反應(Real-time PCR) ────────20
2.14 TA Cloning與轉形作用(Transformation) ───────21
2.15 質體DNA的微量萃取────────────────────22
2.16 核酸限制酶切割(Restriction Enzyme Cutting) ─────22
2.17 接合作用(Ligation) ──────────────────22
2.18 誘導作用(Induction) ─────────────────23
2.19 以尿素處理不溶性蛋白質─────────────────23
2.20 以8M尿素平衡後的鎳親合性管柱純化不溶性蛋白質──────24
2.21 SDS–聚丙烯醯胺蛋白質膠體電泳
(SDS-Polyacrylamide Gel Electrophoresis, SDS-PAGE)──24
2.22 銀染(Silver Stain) ───────────────────25
2.23 西方轉漬法(Western Blotting) ──────────────26
2.24 蛋白質的分解(Protein Digestion)和MALDI-TOF MS ─────26

§ 第三章 實驗結果───────────────────28
3.1 陰道鞭毛蟲tv_arf1a基因序列的取得──────────28
3.2陰道鞭毛蟲Tv_Arf1a的序列分析─────────────29
3.3利用北方墨點法偵測tv_arf1a mRNA的表達────────32
3.4利用同步定量聚合酶連鎖反應偵測tv_arf異構物表達差異──32
3.5 Tv_Arf1a的基因選殖與重組蛋白質的純化─────────34
3.6 利用雷射脫附游離飛行時間式質譜儀(MALDI-TOF mass spectrometry)鑑定Tv_Arf1a重組蛋白質────────────────────36
3.7 利用Tv_Arf1a多株抗體偵測陰道鞭毛蟲體內Tv_Arf1a蛋白質───37

§ 第四章 討論與結論──────────────────38

§ 參考文獻──────────────────────43

§ 圖表────────────────────────52
圖1:陰道鞭毛蟲的型態─────────────────52
圖2:陰道鞭毛蟲的生活史────────────────53
圖3:GEF/GEP和GAP在Arf的活化態與非活化態間所扮演的角色────54
圖4:Arf和Arf-like蛋白質受核苷酸的結合而改變構型,證明Arf家族的interswitch toggle機制───────────────────────────55
圖5:磷酸酯解酶D在COPI囊泡的形成上,提供一個回饋的調控機制───56
圖6:COPI囊泡產生的過程────────────────────57
圖7:Arf1調控的位置與作用蛋白質(effectors) ─────────58
圖8:陰道鞭毛蟲Tv_Arf1a開放讀框的PCR產物───────────59
圖9: tv_arf1a的cDNA序列全長以及胺基酸序列──────────60
圖10:Tv_Arf1a的荳蔻酸化轉錄後修飾預測────────────61
圖11:Tv_Arf1a在陰道鞭毛蟲體內分布位置的預測─────────62
圖12:Tv_Arf1a與Homo sapiens及Mus musculus Arfs胺基酸序列相似度比較─63
圖13:Tv_Arf1a與Homo sapiens和Mus musculus Arfs進行多重序列(multi-alignment)比對────────────────────────64
圖14:Arfs的3D結構預測────────────────────65
圖15:利用樹狀圖來表示Tv_Arf1a與其它陰道鞭毛蟲體內Arf異構物 的胺基酸序列相似度──────────────────────66
圖16:藉由北方墨點法確認tv_arf1a mRNA的表現─────────67
圖17:利用同步定量聚合酶連鎖反應偵測單一Arf在不同時間點表達量差異─68
圖18:比較陰道鞭毛蟲被培養在正常環境與含有纖維連接蛋白下,不同時間點Arf異構物的表達量差異───────────────────────────69
圖19:重組蛋白質His-tagged Tv_Arf1a的表達──────────70
圖20:His-tagged Tv_Arf1a重組蛋白質被送入大腸桿菌大量表達並透過西方轉漬法進行偵測─────────────────────────────71
圖21:利用鎳親合性管柱純化Histidine-tagged Tv_Arf1a重組蛋白質──72
圖22:使用飛行式質譜儀(MALDI-TOF mass spectra)分析純化出的His-tagged Tv_ARF1a重組蛋白質─────────────────────────73
圖23:利用Tv_Arf1a的多株抗體偵測陰道鞭毛蟲體內Tv_Arf1a蛋白質 ───74
1.Lyons EJ, Carlton JM. Mind the gap: bridging the divide between clinical and molecular studies of the trichomonads. Trends Parasitol. 2004; 20:204-207.
2.Rein, M. F. 1990. Clinical manifestations of urogenital trichomoniasis in women, p. 225–234. In B. M. Honigberg (ed.), Trichomonads parasitic in humans. Springer-Verlag, New York, N.Y.
3.Sorvillo F, Smith L, Kerndt P, Ash L. Trichomonas vaginalis, HIV, and African-Americans. Emerg. Infect. Dis. 2001; 7:927-932.
4.Upcroft P, Upcroft JA. Drug targets and mechanisms of resistance in the anaerobic protozoa. Clin. Microbiol. Rev. 2001; 14:150-164.
5.Honigberg BM, King VM. Structure of Trichomonas vaginalis Donne. J. Parasitol. 1964; 50:345-364.
6.Arroyo R, González-Robles A, Martínez-Palomo A, Alderete JF. Signalling of Trichomonas vaginalis for amoeboid transformation and adhesion synthesis follows cytoadherence. Mol. Microbiol. 1993; 7:299-309.
7.Heath JP. Behaviour and pathogenicity of Trichomonas vaginalis in epithelial cell cultures: a study by light and scanning electron microscopy. Br. J. Vener. Dis. 1981; 57:106-117.
8.Müller M. Hydrogenosomes of trichomonad flagellates. Acta Univ. Carol. Biol. 1987; 30:249-260.
9.Müller M. The hydrogenosome. J. Gen. Microbiol. 1993; 139:2879-2889.
10.Brugerolle G. Etude de la cryptopleuromitose et de la morphogenese de division chez Trichomonas vaginalis et chez pleusiers genres de trichomonadines primitives. Protistologica. 1975; 11:457-468.
11.Benchimol M, Almeida JC, de Souza W. Fine structure and cytochemistry of the hydrogenosome of Tritrichomonas foetus. J. Protozool. 1983; 30:422-425.
12.Benchimol M, Johnson PJ, de Souza W. Morphogenesis of the hydrogenosome: an ultrastructural study. Biol. Cell. 1996; 87:197-205.
13.Honigberg BM, Volkmann D, Entzeroth R, Scholtyseck E. A freeze-fracture electron microscope study of Trichomonas vaginalis Donne and Tritrichomonas foetus (Riedmuller). J. Protozool. 1984; 31:116-131.
14.Johnson PJ, Lahti CJ, Bradley PJ. Biogenesis of the hydrogenosome in the anaerobic protist Trichomonas vaginalis. J. Parasitol. 1993; 79:664-670.
15.Attardi G, Schatz G. Biogenesis of mitochondria. Annu. Rev. Cell. Biol. 1988; 4:289-333.
16.Heine P, McGregor JA. Trichomonas vaginalis: a reemerging pathogen. Clin. Obstet. Gynecol. 1993; 36:137-144.
17.Honigberg BM, Brugerolle G. 1990. Structure, p. 5–35. In B. M. Honigberg. (ed.), Trichomonads parasitic in humans. Springer-Verlag, New York, N.Y.
18.Brugerolle G. Etude de la cryptopleuromitose et de la morphogenese de division chez Trichomonas vaginalis et chez pleusiers genres de trichomonadines primitives. Protistologica. 1975; 11:457-468.
19.Heine P, McGregor JA. Trichomonas vaginalis: a reemerging pathogen. Clin. Obstet. Gynecol. 1993; 36:137-144.
20.Huggins GR, Preti G. Vaginal odours and secretions. Clin. Obstet. Gynecol. 1981; 24:355-376.
21.Coombs GH, Westrop GD, Suchan P, Puzova G, Hirt RP, Embley TM, Mottram JC, Muller S. The amitochondriate eukaryote Trichomonas vaginalis contains a divergent thioredoxin-linked peroxiredoxin antioxidant system. J. Biol. Chem. 2004; 279:5249-5256.
22.al-Salihi FL, Curran JP, Wang J. Neonatal Trichomonas vaginalis: report of three cases and review of the literature. Pediatrics. 1974; 53:196-200.
23.Marchetti E, Fedi B, Tanini R, Pelini G. Statistical observations on the incidence of Trichomonas vaginalis in the female population of Terni from 1974 to 1978. Boll. Soc. Ital. Biol. Sper. 1979; 15:1295-1299.
24.Spiegel CA. 1990. Microflora associated with Trichomonas vaginalis and vaccination against vaginal trichomoniasis, p. 213–224. In B. M. Honigberg (ed.), Trichomonads parasitic in humans. Springer-Verlag, New York, N.Y.
25.Sorvillo F, Smith L, Kerndt P, Ash L. Trichomonas vaginals, HIV, and African-Americans. Emerg. Infect. Dis. 2001; 7:927-932.
26.Cameron DW, Padian NS. Sexual transmission of HIV and the epidemiology of other sexually transmitted diseases. AIDS. 1990; 4:99-103.
27.Laga M, Manoka A, Kivuvu M, Malele B, Tuliza M, Nzila N, Goeman J, Behets F, Batter V, Alary M, et al. Non-ulcerative sexually transmitted diseases as risk factors for HIV-1 transmission in women: results from a cohort study. AIDS. 1993; 7:95-102.
28.Donaldson JG. Arfs, phosphoinositides and membrane traffic. Biochem. Soc. Trans. 2005; 33:1276-1278.
29.Randazzo PA, Weiss O, Kahn RA. Preparation of recombinant ADP-ribosylation factor. Methods. Enzymol. 1992; 219:362-369.
30.Sewell JL, Kahn RA. Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins. Proc. Natl. Acad. Sci. U S A. 1988; 85:4620-4624.
31.Takai Y, Sasaki T, Matozaki T. Small GTP-binding proteins. Physiol. Rev. 2001; 81:153-208.
32.Fischer R, Wei Y, Anagli J, Berchtold MW. Calmodulin binds to and inhibits GTP binding of the ras-like GTPase Kir/Gem. J. Biol. Chem. 1996; 271:25067-25070.
33.Kahn RA, Cherfils J, Elias M, Lovering RC, Munro S, Schurmann A. Nomenclature for the human Arf family of GTP-binding proteins: ARF, ARL, and SAR proteins. J. Cell. Biol. 2006; 172:645-650.
34.Volpicelli-Daley LA, Li Y, Zhang CJ, Kahn RA. Isoform-selective effects of the depletion of ADP-ribosylation factors 1-5 on membrane traffic. Mol. Biol. Cell. 2005; 16:4495-508.
35.D'Souza-Schorey C, Chavrier P. ARF proteins: roles in membrane traffic and beyond. Nat. Rev. Mol. Cell. Biol. 2006; 7:347-358.
36.Logsdon J, Kahn RA, in: R.A. Kahn (Ed.), Arf Family GTPases,
Kluwer Academic Publishers, Boston, MA, 2003, pp. 1 –21.
37.Peyroche A, Paris S, Jackson CL. Nucleotide exchange on ARF mediated by yeast Gea1 protein. Nature. 1996; 384:479-481.
38.Chardin P, Paris S, Antonny B, Robineau S, Beraud-Dufour S, Jackson CL, Chabre M. A human exchange factor for ARF contains Sec7- and pleckstrin-homology domains. Nature. 1996; 384:481-484.
39.Jackson CL, Casanova JE. Turning on ARF: the Sec7 family of guanine-nucleotide-exchange factors. Trends. Cell. Biol. 2000; 10:60-67.
40.Poon PP, Wang X, Rotman M, Huber I, Cukierman E, Cassel D, Singer RA, Johnston GC. Saccharomyces cerevisiae Gcs1 is an ADP-ribosylation factor GTPase-activating protein. Proc. Natl. Acad. Sci. U S A. 1996; 93:10074-10077.
41.Randazzo PA, Kahn RA. GTP hydrolysis by ADP-ribosylation factor is dependent on both an ADP-ribosylation factor GTPaseactivating protein and acid phospholipids. J. Biol. Chem. 1994; 269:10758-10763.
42.Makler V, Cukierman E, Rotman M, Admon A, Cassel D. ADP-ribosylation factor-directed GTPase-activating protein purification and partial characterization. J. Biol. Chem. 1995; 270:5232-5237.
43.Burd CG, Strochlic TI, Gangi Setty SR. Arf-like GTPases: not so Arf-like after all. Trends. Cell. Biol. 2004; 14:687-694.
44.Pasqualato S, Renault L, Cherfils J. Arf, Arl, Arp and Sar proteins: a family of GTP-binding proteins with a structural device for 'front-back' communication. EMBO Rep. 2002; 3:1035-1041.
45.Cavenagh MM, Whitney JA, Carroll K, Zhang C, Boman AL, Rosenwald AG, Mellman I, Kahn RA. Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane. J. Biol. Chem. 1996; 271:21767-21774.
46.Balch WE, Kahn RA, Schwaninger R. ADP-ribosylation factor is required for vesicular trafficking between the endoplasmic reticulum and the cis-Golgi compartment. J. Biol. Chem. 1992; 267:13053-13061.
47.Liang JO, Kornfeld S. Comparative activity of ADP-ribosylation factor family members in the early steps of coated vesicle formation on rat liver Golgi membranes. J. Biol. Chem. 1997; 272:4141-4148.
48.Sturm NR, Van Valkenburgh H, Kahn RA, Campbell DA. Characterization of a GTP-binding protein in the ADP-ribosylation factor subfamily from Leishmania tarentolae. Biochim. Biophys. Acta. 1998; 1442:347-352.
49.Brown HA, Gutowski S, Moomaw CR, Slaughter C, Sternweis PC. ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase D activity. Cell. 1993; 75:1137-1144.
50.Cockcroft S, Thomas GM, Fensome A, Geny B, Cunningham E, Gout I, Hiles I, Totty NF, Truong O, Hsuan JJ. Phospholipase D: a downstream effector of ARF in granulocytes. Science. 1994; 263:523-526.
51.Honda A, Nogami M, Yokozeki T, Yamazaki M, Nakamura H, Watanabe H, Kawamoto K, Nakayama K, Morris AJ, Frohman MA, Kanaho Y. Phosphatidylinositol 4-phosphate 5-kinase alpha is a downstream effector of the small G protein ARF6 in membrane ruffle formation. Cell. 1999; 99:521-532.
52.Price HP, Goulding D, Smith DF. ARL1 has an essential role in Trypanosoma brucei. Biochem. Soc. Trans. 2005; 33:643-645.
53.Tamkun JW, Kahn RA, Kissinger M, Brizuela BJ, Rulka C, Scott MP, Kennison JA. The arflike gene encodes an essential GTP-binding protein in Drosophila. Proc. Natl. Acad. Sci. U S A. 1991; 88:3120-3124.
54.Morgan GW, Allen CL, Jeffries TR, Hollinshead M, Field MC. Developmental and morphological regulation of clathrin-mediated endocytosis in Trypanosoma brucei. J. Cell. Sci. 2001; 114:2605-2615.
55.Lee FJ, Huang CF, Yu WL, Buu LM, Lin CY, Huang MC, Moss J, Vaughan M. Characterization of an ADP-ribosylation factor like 1 protein in Saccharomyces cerevisiae. J. Biol. Chem. 1997; 272:30998-31005.
56.Rudolph R, Lilie H. In vitro folding of inclusion body proteins. FASEB J. 1996; 10:49-56.
57.Fahnert B, Lilie H, Neubauer P. Inclusion bodies: formation and utilisation. Adv. Biochem. Eng. Biotechnol. 2004; 89:93-142.
58.Yanagida M, Miura Y, Yagasaki K, Taoka M, Isobe T, Takahashi N. Matrix assisted laser desorption/ionization-time of flight-mass spectrometry analysis of proteins detected by anti-phosphotyrosine antibody on two-dimensional-gels of fibrolast cell lysates after tumor necrosis factor-alpha stimulation. Electrophoresis. 2000; 21:1890-1898.
59.Sturm NR, Van Valkenburgh H, Kahn RA, Campbell DA. Characterization of a GTP-binding protein in the ADP-ribosylation factor subfamily from Leishmania tarentolae. Biochim. Biophys. Acta. 1998; 1442:347-352.
60.Cuvillier A, Redon F, Antoine JC, Chardin P, DeVos T, Merlin G. LdARL-3A, a Leishmania promastigote-specific ADP-ribosylation factor-like protein, is essential for flagellum integrity. J. Cell. Sci. 2000; 113:2065-2074.
61.Lee FJ, Moss J, Vaughan M. Human and Giardia ADP-ribosylation factors (ARFs) complement ARF function in Saccharomyces cerevisiae. J. Biol. Chem. 1992; 267:24441-24445.
62.Liendo A, Stedman TT, Ngo HM, Chaturvedi S, Hoppe HC, Joiner KA. Toxoplasma gondii ADP-ribosylation factor 1 mediates enhanced release of constitutively secreted dense granule proteins. J. Biol. Chem. 2001; 276:18272-18281.
63.de Sá-Freire A, Nepomuceno-Silva JL, da Paixão JC, de Mendonça SM, de Melo LD, Lopes UG. TcArf1: a Trypanosoma cruzi ADP-ribosylation factor. Parasitol. Res. 2003; 91:166-170.
64.Honigberg BM, Brugerolle G. in: Honigberg, B. M. (Ed.), Trichomonads Parasitic in Humans, Springer-Verlag, New York 1990, pp. 5–35.
65.Peterson KM, Alderete JF. Iron uptake and increased intracellular enzyme activity follow host lactoferrin binding by Trichomonas vaginalis receptors. J. Exp. Med. 1984; 160:398-410.
66.Lehker MW, Chang TH, Dailey DC, Alderete JF. Specific erythrocyte binding is an additional nutrient acquisition system for Trichomonas vaginalis. J. Exp. Med. 1990; 171:2165-2170.
67.Petrin D, Delgaty K, Bhatt R, Garber G. Clinical and microbiological aspects of Trichomonas vaginalis. Clin. Microbiol. Rev. 1998; 11:300-317.
68.Moss J, Vaughan M. Activation of toxin ADP-ribosyltransferases by eukaryotic ADP-ribosylation factors. Mol. Cell. Biochem. 1999; 193:153-157.
69.Michael GR, Kun B, Nicholas TK, Sidney Y. Phospholipase D as an effector for ADP-ribosylation factor in the regulation of vesicular traffic. Chem. Phys. Lipids. 1999; 98:141-152.
70.Spang A. ARF1 regulatory factors and COPI vesicle formation. Curr. Opin. Cell. Biol. 2002; 14:423-427.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關論文
 
1. 天涼無心。〈現代人的「眾生相」電影《神隱少女》的線索與思考〉。《國文天地》。(2002年第17卷12期)。頁78-84。
2. 方振寧。〈動畫 人類意識擴張的媒體—日本動畫藝術的崛起〉。《藝術家》。(2003年第337期)。頁332-347。
3. 吳知賢。〈電視媒體中兩性關係的探討〉。《國教之友》。(1999年第51卷第1期)。頁3-11。
4. 吳知賢。〈電視節目中兩性知識及性內容的探討〉。《視聽教育雙月刊》。(1998年第36卷第六期)。頁23-32。
5. 沈秀貞。〈幻境與人境的相映-談宮崎駿新作《神隱少女》2002年柏林影展首獎「金熊獎」得獎作品〉。《人本教育札記》。(1999年第153期)。頁101-105。
6. 傅伯寧。〈宮崎駿談卡通〉。《人本教育札記》。(2002年第152期)。頁26。
7. 曾素秋。〈性別角色的轉變與新兩性關係〉。《高市文教》。(1997年第59期)。頁4-8。
8. 湯禎兆。〈《神隱少女》的大千世界〉。《電影欣賞》。(2001年20:3=111)。頁68-69。
9. 劉森雨。〈「魔法公主」裡的生態和女性自覺〉。《張老師月刊》。(1997年第237期)。頁35-39。
10. 鄭彩仁。〈繁複精緻的百寶箱-電影神隱少女魅力無法擋〉。《明道文藝》。(2002年第318期)。頁15-20。
11. 藍祖蔚。〈走。我們和宮崎駿一起旅行去!〉。《人本教育札記》。(2002年第152期)。頁16-19。
12. 顏忠賢。〈一個薛西佛斯式的行動—宮崎駿作品的「飛行器」分析〉。《影響電影雜誌》。(1993年第40期)。頁137-143。