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研究生:郭亭坊
研究生(外文):Ting-Fang Kuo
論文名稱:利用RNA干擾技術抑制人類乳突瘤病毒引起的子宮頸癌細胞生長
論文名稱(外文):The feasibility of using HPV-RNAi on the inhibition of carcinogenesis in cervical cancer cells
指導教授:鄭恩加
指導教授(外文):Ann-Joy Cheng
學位類別:碩士
校院名稱:長庚大學
系所名稱:生化與生醫工程研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:英文
論文頁數:70
中文關鍵詞:干擾人類乳突瘤病毒子宮頸癌基因療法
外文關鍵詞:RNAisiRNAHPVcevrical cancer
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子宮頸癌是全球婦女第二常見的癌症,在發展中國家如台灣,更是排名婦女癌症的首位,其發生原因與人類乳突瘤病毒(HPV)之感染有密切的關係。HPV所攜帶的致癌基因E6和E7能破壞體內抑癌蛋白p53和pRb的表現並導致細胞癌化。考量到子宮頸在生殖上的重要地位以及傳統癌症治療方法所引發的後遺症,本研究希望能利用RNA干擾技術(RNAi)來專一性地抑制HPV致癌基因E6、E7之表現,同時達到抑制癌細胞生長的效果。
研究方法是採用有HPV感染的細胞株CaSki (HPV­16) 及HeLa (HPV­18),分別針對其E6、E7的序列設計互補的siRNA (16E6/16E7;18E6/18E7共4種),經轉染到細胞株之後在細胞和分子層次確認siRNA的抑制效果。結果觀察到經siRNA轉染的細胞其E6、E7 mRNA表現量有受到抑制,而後更發現細胞的生長明顯停滯甚至邁向凋亡,至於轉染vector或是沒有HPV感染的細胞株C33A (HPV negative control) 則幾乎不受影響,進一步的動物實驗也發現有施打siRNA的腫瘤在生長速度以及體積上都明顯比較小。由以上結果可看出這些siRNA的確能專一性地抑制E6、E7表現並促進腫瘤細胞凋亡,也對未來RNAi的應用提供更多學理上的基礎。
Cervical cancer is the second most common malignancy among women worldwide. Previous infections with high-risk types of human papillomaviruses (HPVs) are a necessary cause of cervical cancer and HPV viral genes E6 and E7 play critical roles for induction of carcinogenesis. E6 protein can target p53 for degradation through the ubiquitin pathway, and E7 protein can inactivate retinoblastoma (pRb).
Giving thought to the importance of cervix and the severe side effects resulting from traditional cancer therapies, this study wants to achieve targeted inhibition of oncogenes in tumor cells based on RNA interference (RNAi).
To this aim, we constructed 4 kinds of siRNAs which are sequence-complementally to each target gene (16E6/16E7; 18E6/18E7). , After transfected these siRNAs into HPV infected cell lines CaSki (HPV-16) and HeLa (HPV-18), we observed downregulation of E6/E7 mRNA and, moreover, obvious decrease in cell growth and increased apoptosis. The vector control or HPV negative cell line C33A were not influenced in comparison. We further proceeded with animal experiments to confirm these siRNAs effects in vivo and found that intra-tumor injection of siRNAs could reduce tumor growth in nude mice. (n=7)
These results suggest that siRNAs indeed have specific inhibition on E6/E7 and accelerate tumor cell death.
摘要……………………………………………………………………...vi

Abstract…………………………………………………………………vii

Content…………………………………………………………………viii

Table list…………………………………………………………………ix

Figure list…………………………………………………………...........x

Background and significance…………………………………………….1

Research design and specific aim……………………………………….

Materials and methods…………………………………………………..

Results and discussion…………………………………………………..

Future works…………………………………………………………….

Reference:……………………………………………………………….

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1. Zhi-Ming Zheng and Carl C. Baker. “Papillomavirus genome structure, expression, and post-transcriptional regulation”, Front Biosci, Author manuscript; available in PMC 2006 May 30
2. Franco EL, Schlecht NF, and Saslow D. “The epidemiology of cervical cancer”, Cancer J, 9(5):348-59, 2003
3. Peter JF Snijders, Renske DM Steenbergen, Danielle AM Heideman and Chris JLM Meijer. “HPV-mediated cervical cancer carcinogenesis: concepts and clinical implication”, J Pathol, 208: 152-164, 2006
4. Karl Munger, PeterM. Howley. Human papillomavirus immortalization and transformation functions. Virus Research 89, 213-228, 2002
5. Woon-Won Jung, Taehoon Chun, Donggeun Sul, Kwang Woo Hwang, Hyung-Sik Kang, Duck Joo Lee, and In-Kwon Han. Strategies Against Human Papillomavirus Infection and Cervical Cancer. The Journal of Microbiology, p.255-266, 2004
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