(44.192.70.216) 您好!臺灣時間:2021/05/09 19:32
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:曹子杰
研究生(外文):Zih-Jay
論文名稱:赭麴毒素A和多摩酸單株抗體的製備及其在免疫化學的應用
論文名稱(外文):Production of monoclonal antibodies against Ochratoxin A and Domoic acid and their application in ELISA and Colloidal gold Immunostrip assay
指導教授:余豐益
指導教授(外文):Feng-Yih Yu
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:生物醫學科學學系碩士班
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:107
相關次數:
  • 被引用被引用:0
  • 點閱點閱:276
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
赭麴毒素A (Ochratoxin A, OTA)為一個廣泛污染飼料和食物的黴菌毒素,它被認為跟巴爾幹半島地區性的腎疾病和上泌尿道的癌症有關。 為了發展更快速且敏感的方法來檢測OTA,本實驗室用免疫OTA-KLH的BALB/c品種老鼠的脾臟細胞與NS-1骨髓瘤細胞融合,篩選得到一個穩定的融合瘤細胞株H9,用來生產抗OTA的單株抗體。 我們用OTA專一性的單株抗體建立了敏感性高的直接競爭型酵素連結免疫吸附分析法和以奈米金粒子為標記物的快速免疫層析試紙分析法。 在cd ELISA中,抑制50%的OTA-HRP與抗體結合所需OTA的濃度(IC50)為0.27 ng/mL。 OTA專一性的快速免疫層析試紙分析法偵測OTA的敏感性可低到5 ng/mL。 由檢測咖啡樣品中OTA含量的結果可看出免疫層析試紙分析法和ELISA的測試結果有很好的一致性,且結果可直接目測並在少於10分鐘內快速完成。 由本研究所建立的以OTA單株抗體為主的cdELISA和免疫層析試紙分析法可用來快速簡便的監控咖啡樣品中的OTA且樣品不需經過純化。

多摩酸(Domoic acid, DA)為一種具有神經刺激性的小分子毒素,主要是由矽藻Pseudonitzschia pungens所產生的二級代謝物,人類誤食被DA污染之貝類後,主要會造成短暫性記憶喪失型中毒,一般稱為Amnesic shellfish poisoning (ASP)症狀,高劑量中毒時甚至會導致死亡。 為了發展更快速且敏感的方法來檢測DA,我們也生產了DA的單株抗體9F1F11,是由免疫DA-KLH的BALB/c品種老鼠的脾臟細胞與NS-1骨髓瘤細胞融合,篩選得到穩定的融合瘤細胞株9F1F11所分泌。 我們用DA專一性的單株抗體建立了敏感性高的直接競爭型酵素連結免疫吸附分析法和以奈米金粒子為標記物的快速免疫層析試紙分析法。 在cd ELISA中,抑制50%的DA-HRP與抗體結合所需DA的濃度(IC50)為0.58 ng/mL。 DA專一性的快速免疫層析試紙分析法偵測DA的敏感性可低到5 ng/mL。 由檢測貽貝樣品中DA含量的結果可看出免疫層析試紙分析法和ELISA的測試結果有很好的一致性,且結果可直接目測並在少於10分鐘內快速完成。


Ochratoxin A (OTA) is a widespread mycotoxin contaminating feed and food, and is suspected of being the etiological agent of Balkan endemic nephropathy (BEN) and its associated urinary tract cancers. To develop more rapid and sensitive methods for detection of OTA, monoclonal antibodies (mAb) against OTA were produced from a stable hybridoma cell line, H9, generated by the fusion of P3/NS-1/1-AG4-1 myeloma cells with spleen cells isolated from BALB/c mouse immunized with OTA-keyhole limpet hemocyanin. The OTA-specific mAb H9 was used for developed a sensitive direct competitive enzyme-linked immunosorbent assay (cdELISA) and a rapid mAb based colloidal gold immunostrip assay. The concentration causing 50% inhibition (IC50) of binding of OTA–horseradish peroxidase with the antibodies by OTA in the cdELISA were found to be 0.27 ng/mL. The detection limit of OTA-specific immunostrip was 5 ng/mL for OTA. The results of immunostrip analysis of OTA in coffee samples were in good agreement with those obtained from cdELISA and the result can be observed directly by the nake eyes, and the detection time could be completed within 10 min. The OTA mAb-based cdELISA and immunostrip assay development in this study is suitable for the simple and rapid screening of OTA in coffee samples without sample cleanup.

Domoic acid (DA) is a naturally occurring neuroexcitatory toxin produced primarily by the marine diatom pseudonitzschia pungens. Human ingested DA contaminated shellfish leads to amnesic shellfish poisoning (ASP), which even cause death at high dose of DA. To develop more rapid and sensitive methods for detection of DA, monoclonal antibodies (mAb) against DA were also development, which was produced from a stable hybridoma cell line, 9F1F11, generated by the fusion of P3/NS-1/1-AG4-1 myeloma cells with spleen cells isolated from BALB/c mouse immunized with DA-keyhole limpet hemocyanin. The DA-specific mAb 9F1F11 were used for developed a sensitive cdELISA and a rapid mAb based colloidal gold immunostrip assay. The concentration causing 50% inhibition (IC50) of binding of DA–horseradish peroxidase with the antibodies by DA in the cdELISA were found to be 0.58 ng/mL. The detection limit of the DA-specific immunostrip was 5 ng/mL for DA. The results of immunostrip analysis of DA in blue mussel samples were in good agreement with those obtained from cdELISA and the result can be observed directly by the nake eyes, and the detection time could be completed within 10 min.



目錄
(Index)

中文摘要 ……………………………………… 2

英文摘要 ……………………………………… 4

序論 ……………………………………… 6

材料與方法 ……………………………………… 21

結果 ……………………………………… 37

討論 ……………………………………… 47

圖表 ……………………………………… 66

參考文獻 ……………………………………… 87

附錄 ……………………………………… 101


參考文獻
(References)

Abramson, D., Mills, J. T., and Sinha, R. N. (1990). Mycotoxin production in amber durum wheat stored at 15 and 19% moisture content. Food Addit Contam 7, 617-627.

Aizawa, M., (1987). Enzyme-linked immunosorbent assays using
oxygen- sensing electrode, Electrochem. Sens. Immunol. Anal. P.269.

Axberg, K., Jansson, G., and Hult, K. (1998). Ochratoxin A in rice cultivars after inoculation of Penicillium verrucosum. Nat Toxins 6, 73-84.

Barna-Vetro, I.; Solti, L.; Teren, J.; Gyongyosi, A.; Szabo, E.; Wolfling, (1996). A Sensitive ELISA Test for Determination of Ochratoxin A. J. Agric. Food Chem., 44, 4070- 4074. J. Agric. Food Chem., 44 (12), 4071 -4074

Bennett, J. W., and Klich, M. (2003). Mycotoxins. Clin Microbiol Rev 16, 497-516.

Berman, F. W., and Murray, T. F. (1997). Domoic acid neurotoxicity in cultured cerebellar granule neurons is mediated predominantly by NMDA receptors that are activated as a consequence of excitatory amino acid release. J Neurochem 69, 693-703.

Berry, C. L. (1988). The pathology of mycotoxins. J Pathol 154, 301-311.

Blesa, J., Berrada, H., Soriano, J. M., Molto, J. C., and Manes, J. (2004). Rapid determination of ochratoxin A in cereals and cereal products by liquid chromatography. J Chromatogr A 1046, 127-131.

Breitholtz-Emanuelsson, A., Olsen, M., Oskarsson, A., Palminger, I., and Hult, K. (1993). Ochratoxin A in cow''s milk and in human milk with corresponding human blood samples. J AOAC Int 76, 842-846.

Brown, M. H., Szczech, G. M., and Purmalis, B. P. (1976). Teratogenic and toxic effects of ochratoxin A in rats. Toxicol Appl Pharmacol 37, 331-338.

Chandler, J., Gurmin, T., and Robinson, N. (2000)"The Place of Gold in Rapid Tests," IVD Technology 6, no. 2: 37–49.

Chernozemsky, I. N., Stoyanov, I. S., Petkova-Bocharova, T. K., Nicolov, I. G., Draganov, I. V., Stoichev, II, Tanchev, Y., Naidenov, D., and Kalcheva, N. D. (1977). Geographic correlation between the occurrence of endemic nephropathy and urinary tract tumours in vratza district, Bulgaria. Int J Cancer 19, 1-11.

Cho, J. H., Paek, E. H., Cho, I. H., and Paek, S. H. (2005a). An enzyme immunoanalytical system based on sequential cross-flow chromatography. Anal Chem 77, 4091-4097.

Cho, Y. J., Lee, D. H., Kim, D. O., Min, W. K., Bong, K. T., Lee, G. G., and Seo, J. H. (2005b). Production of a monoclonal antibody against ochratoxin A and its application to immunochromatographic assay. J Agric Food Chem 53, 8447-8451.

Chu, F. S. (1991). Mycotoxins: food contamination, mechanism, carcinogenic potential and preventive measures. Mutat Res 259, 291-306.

Clayden, J., Knowles, F. E., and Baldwin, I. R. (2005). The synthesis of (-)-isodomoic acid C. J Am Chem Soc 127, 2412-2413.

Clarke, J. R.; Marquardt, R. R.; Oosterveld, A.; Frohlich, A.; Madrid, F. J.; Dawood, M. (1993). Development of a Quantitative and Sensitive Enzyme-Linked Immunosorbent Assay for Ochratoxin A Using Antibody from the Yolk of the Laying Hen. J. Agric. Food Chem., 41, 1784-1789.

Creppy, E. E., Kane, A., Dirheimer, G., Lafarge-Frayssinet, C., Mousset, S., and Frayssinet, C. (1985). Genotoxicity of ochratoxin A in mice: DNA single-strand break evaluation in spleen, liver and kidney. Toxicol Lett 28, 29-35.

Creppy, E. E., Kern, D., Steyn, P. S., Vleggaar, R., Roschenthaler, R., and Dirheimer, G. (1983). Comparative study of the effect of ochratoxin A analogues on yeast aminoacyl-tRNA synthetases and on the growth and protein synthesis of hepatoma cells. Toxicol Lett 19, 217-224.

Creppy, E. E., Lugnier, A. A., Fasiolo, F., Heller, K., Roschenthaler, R., and Dirheimer, G. (1979). In vitro inhibition of yeast phenylalanyl-tRNA synthetase by ochratoxin A. Chem Biol Interact 24, 257-261.

De Saeger, S., and Van Peteghem, C. (1999). Flow-through membrane-based enzyme immunoassay for rapid detection of ochratoxin A in wheat. J Food Prot 62, 65-69.

Dirheimer, G. (1996). Mechanistic approaches to ochratoxin toxicity. Food Addit Contam 13 Suppl, 45-48.

Doucette, T. A., Strain, S. M., Allen, G. V., Ryan, C. L., and Tasker, R. A. (2000). Comparative behavioural toxicity of domoic acid and kainic acid in neonatal rats. Neurotoxicol Teratol 22, 863-869.

Faulk, W.; Taylor, G. M. (1971). An immunocolloidal method for
electron microscope. Immunocytochemistry, 8, 1081-1083.

Fernandez, M. L., Cembella, A. D. (1995). Mammalian bioassay. In: Manual on Harmful Marine Microalgae, Hallegraeff, G. M., Anderson, D. M., Cembella, A. D. (Eds.), Intergovernmental Oceanographic Commission, Manuals and Guides 33: 213-228. UNESCO, Paris.

Fukal, L. (1990). A survey of cereals, cereal products, feedstuffs and porcine kidneys for ochratoxin A by radioimmunoassay. Food Addit Contam 7, 253-258.

Galtier, P., Alvinerie, M., and Charpenteau, J. L. (1981). The pharmacokinetic profiles of ochratoxin A in pigs, rabbits and chickens. Food Cosmet Toxicol 19, 735-738.

Garthwaite, I., Ross, K. M., Miles, C. O., Hansen, R. P., Foster, D., Wilkins, A. L., and Towers, N. R. (1998). Polyclonal antibodies to domoic acid, and their use in immunoassays for domoic acid in sea water and shellfish. Nat Toxins 6, 93-104.

Gilani, S. H., Bancroft, J., and Reily, M. (1978). Teratogenicity of ochratoxin A in chick embryos. Toxicol Appl Pharmacol 46, 543-546.

Gyongyosi-Horvath, A., Barna-Vetro, I., and Solti, L. (1996). A new monoclonal antibody detecting ochratoxin A at the picogram level. Lett Appl Microbiol 22, 103-105.

Hallen, I. P., Breitholtz-Emanuelsson, A., Hult, K., Olsen, M., and Oskarsson, A. (1998). Placental and lactational transfer of ochratoxin A in rats. Nat Toxins 6, 43-49.

Hamilton, P. B., Huff, W. E., Harris, J. R., and Wyatt, R. D. (1982). Natural occurrences of ochratoxicosis in poultry. Poult Sci 61, 1832-1841.

Hampson, D. R., Huang, X. P., Wells, J. W., Walter, J. A., and Wright, J. L. (1992). Interaction of domoic acid and several derivatives with kainic acid and AMPA binding sites in rat brain. Eur J Pharmacol 218, 1-8.

Hayes, A. W., Hood, R. D., and Lee, H. L. (1974). Teratogenic effects of ochratoxin A in mice. Teratology 9, 93-97.

Harwig, J., Kuiper-Goodman, T. and Scott, P.M. (1983). Microbial food
toxicants:ochratoxins. In: Handbook of Foodborne Diseases of Biological
Origin. Reichcigl, M. (ed.). CRC Press, Boca Raton, Florida, 193-238.

Holland, P. T., McNabb, P., Selwood, A. I., and Neil, T. (2003). Amnesic shellfish poisoning toxins in shellfish: estimation of uncertainty of measurement for a liquid chromatography/tandem mass spectrometry method. J AOAC Int 86, 1095-1100.

Horner, R.A., Garrison, D.L., Plumley, F.G., (1997). Harmful algal blooms and red tide problems on the United States west coast. Limnology and Oceanography 42, 1076–1088.

Inada, M., Nishikawa, M., Naito, K., Oishi, M., Kurata, S., and Imura, H. (1980). Triiodothyronine-binding immunoglobulin in a patient with Grave''s disease and its effect on metabolism and radioimmunoassay of triiodothyronine. Am J Med 68, 787-792.

Jin, Y., Jang, J. W., Lee, M. H., and Han, C. H. (2006). Development of ELISA and immunochromatographic assay for the detection of neomycin. Clin Chim Acta 364, 260-266.

John A. Walter, Michael Falk, and Jeffrey L.C. (1994). Wright Chemistry of the shellfish toxin domoic acid: characterization of related compounds
Can. J. Chem./Rev. can. chim. 72(2): 430-436

Jorgensen, K. (2005). Occurrence of ochratoxin A in commodities and processed food--a review of EU occurrence data. Food Addit Contam 22 Suppl 1, 26-30.

Kawamura, O., Sato, S., Kajii, H., Nagayama, S., Ohtani, K., Chiba, J., and Ueno, Y. (1989). A sensitive enzyme-linked immunosorbent assay of ochratoxin A based on monoclonal antibodies. Toxicon 27, 887-897.

Kawatsu, K., Hamano, Y., and Noguchi, T. (1999). Production and characterization of a monoclonal antibody against domoic acid and its application to enzyme immunoassay. Toxicon 37, 1579-1589.

Kawatsu, K., Hamano, Y., and Noguchi, T. (2000). Determination of domoic acid in Japanese mussels by enzyme immunoassay. J AOAC Int 83, 1384-1386.

Krogh, P., Hald, B., and Pedersen, E. J. (1973). Occurrence of ochratoxin A and citrinin in cereals associated with mycotoxic porcine nephropathy. Acta Pathol Microbiol Scand [B] Microbiol Immunol 81, 689-695.

Kuiper-Goodman, T., and Scott, P. M. (1989). Risk assessment of the mycotoxin ochratoxin A. Biomed Environ Sci 2, 179-248.

Kwak, B. Y.; Shon, D. H. (2000). Detection of Ochratoxin A in Agricultural Commodities Using Enzyme-Linked Immunosorbent Assay. Food Sci. Biotechnol. 9, 168-173.

Lawrence, J. F., Charbonneau, C. F., and Menard, C. (1991). Liquid chromatographic determination of domoic acid in mussels, using AOAC paralytic shellfish poison extraction procedure: collaborative study. J Assoc Off Anal Chem 74, 68-72.

Lea, T., Steien, K., and Stormer, F. C. (1989). Mechanism of ochratoxin A-induced immunosuppression. Mycopathologia 107, 153-159.

Lee, S. C., and Chu, F. S. (1984). Enzyme-linked immunosorbent assay of ochratoxin A in wheat. J Assoc Off Anal Chem 67, 45-49.

Lefebvre, K. A., Bargu, S., Kieckhefer, T., and Silver, M. W. (2002). From sanddabs to blue whales: the pervasiveness of domoic acid. Toxicon 40, 971-977.

Levi, C. P., Trenk, H. L., and Mohr, H. K. (1974). Study of the occurrence of ochratoxin A in green coffee beans. J Assoc Off Anal Chem 57, 866-870.

Levings, C. D., Stein, J. E., Stehr, C. M., and Samis, S. C. (2004). Introduction to the PICES practical workshop: objectives, overview of the study area, and projects conducted by the participants. Mar Environ Res 57, 3-18.

Li, S., Marquardt, R. R., Frohlich, A. A., Vitti, T. G., and Crow, G. (1997). Pharmacokinetics of ochratoxin A and its metabolites in rats. Toxicol Appl Pharmacol 145, 82-90.

Maaroufi, K., Achour, A., Hammami, M., el May, M., Betbeder, A. M., Ellouz, F., Creppy, E. E., and Bacha, H. (1995). Ochratoxin A in human blood in relation to nephropathy in Tunisia. Hum Exp Toxicol 14, 609-614.

Marquardt, R. R., and Frohlich, A. A. (1992). A review of recent advances in understanding ochratoxicosis. J Anim Sci 70, 3968-3988.

Martin, J.L., Haya, K., Burridge, L.E., Wildish, D.J., (1990). Nitzschia pseudodelicatissima - a source of domoic acid in the Bay of Fundy, eastern Canada. Marine Ecology Progress Series 67, 177–182.

Medina, A., Jimenez, M., Gimeno-Adelantado, J. V., Valle-Algarra, F. M., and Mateo, R. (2005). Determination of ochratoxin A in beer marketed in Spain by liquid chromatography with fluorescence detection using lead hydroxyacetate as a clean-up agent. J Chromatogr A 1083, 7-13.

Meisner, H., and Meisner, P. (1981). Ochratoxin A, an in vivo inhibitor of renal phosphoenolpyruvate carboxykinase. Arch Biochem Biophys 208, 146-153.

Monaci, L., and Palmisano, F. (2004). Determination of ochratoxin A in foods: state-of-the-art and analytical challenges. Anal Bioanal Chem 378, 96-103.

Morgan, M. R., McNerney, R., and Chan, H. W. (1983). Enzyme-linked immunosorbent assay of ochratoxin A in barley. J Assoc Off Anal Chem 66, 1481-1484.

Mos, L. (2001). Domoic acid: a fascinating marine toxin. Environ Toxicol Pharmacol 9, 79-85.

Muller, G., Burkert, B., Moller, U., Diller, R., Rohrmann, B., Rosner, H., and Kohler, H. (2004). Ochratoxin A and some of its derivatives modulate radical formation of porcine blood monocytes and granulocytes. Toxicology 199, 251-259.

Muller, G., Kielstein, P., Rosner, H., Berndt, A., Heller, M., and Kohler, H. (1999). Studies of the influence of ochratoxin A on immune and defence reactions in weaners. Mycoses 42, 495-505.

Newsome, H., Truelove, J., Hierlihy, L., and Collins, P. (1991). Determination of domoic acid in serum and urine by immunochemical analysis. Bull Environ Contam Toxicol 47, 329-334.
Omar, R. F., Hasinoff, B. B., Mejilla, F., and Rahimtula, A. D. (1990). Mechanism of ochratoxin A stimulated lipid peroxidation. Biochem Pharmacol 40, 1183-1191.

Osada, M., Marks, L. J., and Stewart, J. E. (1995). Determination of domoic acid by two different versions of a competitive enzyme-linked immunosorbent assay (ELISA). Bull Environ Contam Toxicol 54, 797-804.

Otteneder, H., and Majerus, P. (2001). Ochratoxin A (OTA) in coffee: nation-wide evaluation of data collected by German Food Control 1995-1999. Food Addit Contam 18, 431-435.

Paek, S. H., Lee, S. H., Cho, J. H., and Kim, Y. S. (2000). Development of rapid one-step immunochromatographic assay. Methods 22, 53-60.

Paepens, C., De Saeger, S., Sibanda, L., Barna-Vetro, I., Anselme, M., Larondelle, Y., and Van Peteghem, C. (2005). Evaluation of fumonisin contamination in cornflakes on the Belgian market by "flow-through" assay screening and LC-MS/MS analyses. J Agric Food Chem 53, 7337-7343.

Papachristou, A., and Markaki, P. (2004). Determination of ochratoxin A in virgin olive oils of Greek origin by immunoaffinity column clean-up and high-performance liquid chromatography. Food Addit Contam 21, 85-92.

Park, J. W., Kim, E. K., Shon, D. H., and Kim, Y. B. (2002). Natural co-occurrence of aflatoxin B1, fumonisin B1 and ochratoxin A in barley and corn foods from Korea. Food Addit Contam 19, 1073-1080.

Pestka, J. J. (1988). Enhanced surveillance of foodborne mycotoxins by immunochemical assay. J Assoc Off Anal Chem 71, 1075-1081.

Petkova-Bocharova, T., Chernozemsky, I. N., and Castegnaro, M. (1988). Ochratoxin A in human blood in relation to Balkan endemic nephropathy and urinary system tumours in Bulgaria. Food Addit Contam 5, 299-301.

Petkova-Bocharova, T., Stoichev, II, Chernozemsky, I. N., Castegnaro, M., and Pfohl-Leszkowicz, A. (1998). Formation of DNA adducts in tissues of mouse progeny through transplacental contamination and/or lactation after administration of a single dose of ochratoxin A to the pregnant mother. Environ Mol Mutagen 32, 155-162.

Petzinger, E., and Ziegler, K. (2000). Ochratoxin A from a toxicological perspective. J Vet Pharmacol Ther 23, 91-98.

Pfohl-Leszkowicz, A., Chakor, K., Creppy, E. E., and Dirheimer, G. (1991). DNA adduct formation in mice treated with ochratoxin A. IARC Sci Publ, 245-253.

Pfohl-Leszkowicz, A., Grosse, Y., Kane, A., Creppy, E. E., and Dirheimer, G. (1993). Differential DNA adduct formation and disappearance in three mouse tissues after treatment with the mycotoxin ochratoxin A. Mutat Res 289, 265-273.

Pittet, A., and Royer, D. (2002). Rapid, low cost thin-layer chromatographic screening method for the detection of ochratoxin A in green coffee at a control level of 10 microg/kg. J Agric Food Chem 50, 243-247.

Puntaric, D., Bosnir, J., Smit, Z., Skes, I., and Baklaic, Z. (2001). Ochratoxin A in corn and wheat: geographical association with endemic nephropathy. Croat Med J 42, 175-180.

Quilliam, M. A., Thomas, K., and Wright, J. L. (1998). Analysis of domoic acid in shellfish by thin-layer chromatography. Nat Toxins 6, 147-152.

Quilliam, M. A., and Wright, J. L. (1989). The amnesic shellfish poisoning mystery. Anal Chem 61, 1053A-1106A.

Rahimtula, A. D., Bereziat, J. C., Bussacchini-Griot, V., and Bartsch, H. (1988). Lipid peroxidation as a possible cause of ochratoxin A toxicity. Biochem Pharmacol 37, 4469-4477.

Ramos, A. J., Labernia, N., Marin, S., Sanchis, V., and Magan, N. (1998). Effect of water activity and temperature on growth and ochratoxin production by three strains of Aspergillus ochraceus on a barley extract medium and on barley grains. Int J Food Microbiol 44, 133-140.

Rousseau, D. M., Candlish, A. G., Slegers, G. A., Van Peteghem, C. H., Stimson, W. H., and Smith, J. E. (1987). Detection of ochratoxin A in porcine kidneys by a monoclonal antibody-based radioimmunoassay. Appl Environ Microbiol 53, 514-518.

Scholin, C. A., Gulland, F., Doucette, G. J., Benson, S., Busman, M., Chavez, F. P., Cordaro, J., DeLong, R., De Vogelaere, A., Harvey, J., et al. (2000). Mortality of sea lions along the central California coast linked to a toxic diatom bloom. Nature 403, 80-84.

Scott, P. M., Van Walbeek, W., Kennedy, B., and Anyeti, D. (1972). Mycotoxins (ochratoxin A, citrinin, and sterigmatocystin) and toxigenic fungi in grains and other agricultural products. J Agric Food Chem 20, 1103-1109.

Seegers, J. C., Bohmer, L. H., Kruger, M. C., Lottering, M. L., and de Kock, M. (1994). A comparative study of ochratoxin A-induced apoptosis in hamster kidney and HeLa cells. Toxicol Appl Pharmacol 129, 1-11.

Shim, W. B., Yang, Z. Y., Kim, J. Y., Choi, J. G., Je, J. H., Kang, S. J., Kolosova, A. Y., Eremin, S. A., and Chung, D. H. (2006). Immunochromatography using colloidal gold-antibody probe for the detection of atrazine in water samples. J Agric Food Chem 54, 9728-9734.

Shotwell, O. L., Hesseltine, C. W., and Goulden, M. L. (1969). Ochratoxin A: occurrence as natural contaminant of a corn sample. Appl Microbiol 17, 765-766.

Shreeve, B. J., Patterson, D. S., Pepin, G. A., Roberts, B. A., and Wrathall, A. E. (1977). Effect of feeding ochratoxin to pigs during early pregnancy. Br Vet J 133, 412-417.
Sibanda, L., De Saeger, S., Barna-Vetro, I., and Van Peteghem, C. (2002). Development of a solid-phase cleanup and portable rapid flow-through enzyme immunoassay for the detection of ochratoxin a in roasted coffee. J Agric Food Chem 50, 6964-6967.

Sibanda, L., De Saeger, S., Bauters, T. G., Nelis, H. J., and Van Peteghem, C. (2001). Development of a flow-through enzyme immunoassay and application in screening green coffee samples for ochratoxin A with confirmation by high-performance liquid chromatography. J Food Prot 64, 1597-1602.

Sierra Beltran, A., Palafox-Uribe, M., Grajales-Montiel, J., Cruz-Villacorta, A., and Ochoa, J. L. (1997). Sea bird mortality at Cabo San Lucas, Mexico: evidence that toxic diatom blooms are spreading. Toxicon 35, 447-453.

Smith, D. S., and Kitts, D. D. (1994). A competitive enzyme-linked immunoassay for domoic acid determination in human body fluids. Food Chem Toxicol 32, 1147-1154.

Smith, D. S.; Kitts, D. D. (1995). Enzyme immunoassay for the determination of domoic acid in mussel extracts. J. Agric. Food Chem., 43, 367-371.

Soleas, G. J., Yan, J., and Goldberg, D. M. (2001). Assay of ochratoxin A in wine and beer by high-pressure liquid chromatography photodiode array and gas chromatography mass selective detection. J Agric Food Chem 49, 2733-2740.

Solti, L., Salamon, F., Barna-Vetro, I., Gyongyosi, A., Szabo, E., and Wolfling, A. (1997). Ochratoxin A content of human sera determined by a sensitive ELISA. J Anal Toxicol 21, 44-48.

Staros, J. V., Wright, R. W., and Swingle, D. M. (1986). Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions. Anal Biochem 156, 220-222.

Stoev, S. D. (1998). The role of ochratoxin A as a possible cause of Balkan endemic nephropathy and its risk evaluation. Vet Hum Toxicol 40, 352-360.

Stoev, S. D., Goundasheva, D., Mirtcheva, T., and Mantle, P. G. (2000). Susceptibility to secondary bacterial infections in growing pigs as an early response in ochratoxicosis. Exp Toxicol Pathol 52, 287-296.

Studer-Rohr, I., Dietrich, D. R., Schlatter, J., and Schlatter, C. (1995). The occurrence of ochratoxin A in coffee. Food Chem Toxicol 33, 341-355.

Tangni, E. K., Ponchaut, S., Maudoux, M., Rozenberg, R., and Larondelle, Y. (2002). Ochratoxin A in domestic and imported beers in Belgium: occurrence and exposure assessment. Food Addit Contam 19, 1169-1179.

Thirumala-Devi, K., Mayo, M. A., Reddy, G., Reddy, S. V., Delfosse, P., and Reddy, D. V. (2000). Production of polyclonal antibodies against ochratoxin A and its detection in chilies by ELISA. J Agric Food Chem 48, 5079-5082.

Todd, E., Avery, G., Grant, G. A., Fenwick, J. C., Chiang, R., and Babiuk, T. (1993). An outbreak of severe paralytic shellfish poisoning in British Columbia. Can Commun Dis Rep 19, 99-102.

USFDA (1998). Fish and Fisheries Products Hazards and Controls Guide. 1st Ed., U.S. Food and Drug Administration, Washington, D.C.

Vale, P., Sampayo, M. A. M (2001). Domoic acid in Portuguese shellfish
and fish. Toxicon, 39, 893-904.

Valenta, H., and Goll, M. (1996). Determination of ochratoxin A in regional samples of cow''s milk from Germany. Food Addit Contam 13, 669-676.

Van der Merwe, K. J., Steyn, P. S., Fourie, L., Scott, D. B., and Theron, J. J. (1965). Ochratoxin A, a toxic metabolite produced by Aspergillus ochraceus Wilh. Nature 205, 1112-1113.

Van Dolah, F. M., Finley, E. L., Haynes, B. L., Doucette, G. J., Moeller, P. D., and Ramsdell, J. S. (1994). Development of rapid and sensitive high throughput pharmacologic assays for marine phycotoxins. Nat Toxins 2, 189-196.

Viani, R. (2002). Effect of processing on ochratoxin A (OTA) content of coffee. Adv Exp Med Biol 504, 189-193.

Wallace, D. C. (1997). Mitochondrial DNA in aging and disease. Sci Am 277, 40-47.

Villac, M.C., Roelke, D.L., Villareal, T.A., Fryxell, G.A., (1993). Comparison of two domoic acid-producing diatoms: a review. Hydrobiologia 269:270, 213–224.

Wang, S., Quan, Y., Lee, N., and Kennedy, I. R. (2006). Rapid determination of fumonisin B1 in food samples by enzyme-linked immunosorbent assay and colloidal gold immunoassay. J Agric Food Chem 54, 2491-2495.

Wei, Y. H., Lu, C. Y., Lin, T. N., and Wei, R. D. (1985). Effect of ochratoxin A on rat liver mitochondrial respiration and oxidative phosphorylation. Toxicology 36, 119-130.

Xiulan, S., Xiaolian, Z., Jian, T., Zhou, J., and Chu, F. S. (2005). Preparation of gold-labeled antibody probe and its use in immunochromatography assay for detection of aflatoxin B1. Int J Food Microbiol 99, 185-194.

Yu, F. Y., Chi, T. F., Liu, B. H., and Su, C. C. (2005). Development of a sensitive enzyme-linked immunosorbent assay for the determination of ochratoxin A. J Agric Food Chem 53, 6947-6953.

Yu, F. Y., Liu, B. H., Chou, H. N., and Chu, F. S. (2002). Development of a sensitive ELISA for the determination of microcystins in algae. J Agric Food Chem 50, 4176-4182.

Yu, F. Y., Liu, B. H., Wu, T. S., Chi, T. F., and Su, M. C. (2004). Development of a sensitive enzyme-linked immunosorbent assay for the determination of domoic Acid in shellfish. J Agric Food Chem 52, 5334-5339.

Zheng Z, Ku ST, Ng WS, Binder J. (2005). A New AgraStripTM Total Aflatoxin Lateral Flow Test Kit. Poster Presentation in Gordon Research Conferences in Mycotoxins & Phycotoxins. Waterville, ME, USA: Colby College, 19–24 June.

周宏農, (1999). 水產品藻源毒素檢測操作手冊, 行政院衛生署, 1p.






















QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關論文
 
系統版面圖檔 系統版面圖檔