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論文名稱(外文):Application of MMP-9 Level in Pleural Fluid and Cholesterol Level in Blood as Markers for Congestive Heart Failure in Women
指導教授(外文):Jer-Yuh Liu
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白分解酵素-2 (MMP-2) 和 -9 的蛋白量表現及血中濃度。其收集
28 位急性心肌梗塞無心臟衰竭病人( Killip I)、27 位急性心肌
梗塞併心臟衰竭病人 (Killip II-III)(B 組) ,利用ELISA 及Zamograpgy等實驗方法,檢查血清中膠蛋白分解酵素-2 和MMP-9 的蛋白量表現及血中濃度,來探討心肌梗塞後併發心臟衰竭過程中,上列因子之濃度變化,藉以了解其可能扮演的角色。結果發現在急性心肌梗塞併心臟衰竭病人中MMP-9 有明顯增加(p<0.01) ,而MMP-2 在兩組卻無明顯增加。高MMP-9 血中濃度和發炎及心肌梗塞的範圍大小無關,而其真正機轉有待更進一步研究。其結論MMP-9 的蛋白量表現及血中濃度增加不僅為急性心肌梗塞後發生心臟衰竭的新指標,也提供此種疾病治療的可能病理機轉。
學生延續上述結果想觀察心臟衰竭病人血清中及胸水中MMP-2和MMP-9蛋白的表現,更進一步探討MMP-2和MMP-9蛋白與心臟衰竭病人性別的相關性。已有文獻報告證實了MMP-2與心臟衰竭發生的相關性,目前的研究已知在心臟衰竭病人的血清檢體中可發現MMP-2濃度;但是高MMP-9 血中濃度和發炎及心肌梗塞的範圍大小無關,而其真正機轉有待更進一步研究。本研究我們以Gelatin zymography的方法,來測定25個心臟衰竭病人MMP-2 和 MMP-9的濃度,其中包括25個胸水檢體和25個血清檢體。MMP-2及MMP-9在基底膜的破壞扮演著重要角色,且與癌症的侵襲及轉移最有關,實驗結果顯示心臟衰竭女性病人血清與其胸水比較可觀察有較高的MMP-9。

Myocardial infarction (MI) is the result, in mostly cases, of the destabilization and rupture of atherosclerotic lesions. The destruction of cardiac tissue resulting from myocardial ischemia could further result in heart failure. It has been suggested that plaque instability may be mediated by matrix metalloproteinase (MMP) family. Studies have identified increased MMP-2 and MMP-9 in human platelets, and acute myocardial infarction patients with elevated MMP-2 and MMP-9 levels. However, the alteration of MMP-2 and MMP-9 from post MI left ventricle remodeling to heart failure remains to be clarified. The purpose of this study is to investigate the serum concentrations and activities of MMP-2 and MMP-9 in the developing heart failure from post MI patients. Twenty eight patients with MI without heart failure (Killip FC I) (group A; compensated) and twenty seven MI patients with heart failure (Killip II-III) (group B; decompensated) were collected to evaluate the serum levels and activities of MMP-2 and MMP-9 by ELISA and Zymography, respectively. It was observed that the both serum levels and activities of MMP-9 significantly increased (P &lt; 0.01) in decompensated group compared to compensated group, but there was no significant difference of serum MMP-2 levels and activities between two groups. The highly elevated serum MMP-9 concentration of decompensated patients is not related with inflammatory or localized infarct area of myocardium and the real mechanisms remain to be revealed. We suggest that the increase of MMP-9 levels and activity may be used as a new marker to diagnose the development of heart failure in patients with post MI, and provide the therapeutic implications in the future.
Students extend the above-mentioned result wanting to observe the depleted patient of heart in the serum and behavior of MMP-2 and MMP-9 albumen in the pleural effusion, go still one step further to probe into MMP-2 , MMP-9 albumen and dependence of the depleted patient''s sex of heart. Have document report verify MMP-2 and heart depleted dependence that take place already, present research know depleted serum of patient examine body can find MMP-2 thickness in heart already; But the ranges of the thickness , inflammation and heart attack size has nothing to do in the high MMP-9 blood, and its real machine rotates and remains to go still one step further to study. This our method with Gelatin zymography of research, come to determine the depleted patient MMP-2 of 25 hearts and thickness of MMP-9, include 25 pleural effusion and examine body and 25 pieces of serum and examine the body. MMP-2 and destruction in the basis membrane of MMP-9 are playing an important role, and have something to do with the invasion and attack and transformation of the cancer most, the experimental result shows the depleted woman patient of heart serum and its pleural effusion can relatively observe higher MMP-9 .

貳、縮寫檢索表................ ....7
捌、參考文獻.................... 54

1.Abou-Raya., Naim, A. and Marzouk, S. Cardiac matrix
remodelling in congestive heart failure: the role of
matrix metalloproteinases. Clin.Invest. Med. 27: 93-
2.Altieri,P. , Brunelli, C., Garibald, S., Nicolino,
A.,Ubaldi, S, ,spallarossa, P., Olivotti, L., rossettin,
P., Barsotti, A . and Ghigliotti, G. Metalloproteinases
2 and 9 are increased in plasma of patients with heart
failure. Eur. J. Cllin. Invest. 33: 648-656, 2003.
3.Beatriz-Alvarez, M.D., Garmen-ruiz, M.D., Pilar-Chacon
M. D, Jose Alvarez, M.D., Carmen-Ruiz, M.D. Serum values
of metalloproteinase-2 and metalloproteinase-9 as
related to unstable plaque and inflammatory cellas in
patients with greater than 70% varotid artery stenosis.
J Vasc. Surg. 40: 469-475, 2004.
4.Caulfield, J . b. and Borg, T.K The collagen network of
the heart. Lab. Invest. 40: 364-372, 1979
5.Cheung, P.Y., Sawicki, G. and Wozniak, M. Matrix
metalloproteinase-2contributes to ischemia-reperfusion
injuryinrhw heart. Circulatio101: 1833-1839, 2000.
6.Etoh, T., Joffs, C. and Deschamps, A. M. Myocardial and
interstitial matrix metalloproteinase activity after
acute myocardial infarction in pig. Am. J. Physiol. Heart
Circ. Physiol. 281: H987-H994, 2001.
7.Fernandez-Patron, C., Martinez-Cuesta, M. A., Salas, E.,
Sawicki, G., Wozniak,M. and Rado, M.W. Differential
regulation of platelet aggregation by Matrix
metalloproteinases-9 and -2. Thromb. Haemost. 82: 1730-
1735, 1999.
8.Gaudron, P., Eilles, C., Kugler, I and Ertl, G.
Progressive left ventricular dysfunction and remodeling
after myocardial infarction: potential mechanisms and
early predictors. Circulation 87: 755-763, 1993.
9.Inkubo, Y., Hanada, H and Inshizaka, H. plasma levels of
Matrix metalloproteinase-9 and tissue inhibitor of
metalloproteinase-1are increased in the coronary
circulation in patients with acute coronary syndrome.
Am. Heart J. 141: 211-217, 2001.
10.Jugdutt, B. I. Prevention of Ventricular remodeling
post myocardial infarction: timing and duration of
therapy. Can. J. Cardiol. 9: 103-114, 1993. 11. Jugdutt,
B. I. Ventricular remodeling after infarction and the
extracellular cpllagen , Matrix : when is enough
enough? Circulation 108:1395-1043,2003.
11.Kaden, J. J., Dempfle, C.E. and Sueselbeck,T. Time-
dependent changes in the plasma concentration of matrix
metalloproteinase 9 after acute myocardial infarction.
Cardiology 99:140-144, 2003.
12.Kai, H., Ikeda, H., Yasukawa, H., Kai, M., Seki, Y. and
kuwahara, F. Peripheral blood levels of matrid
metalloprotinases-2and-9 are elevated in patients with
acute coronary syndromes. J. Am. Coll cardiol. 32: 368-
372, 1998.
13.Lu, L., Gunja-Smith, Z. and Woessner, J. F. Matrix
metalloproteinase and collagen ultrastructure in
moderate myocardial ischemia and reperfusion in vivo.
Am. J. Physiol. Heart Circ. Physiol. 279: H601-H609,
14.Nishikawa, N., Yamamoto, K. and Sakata, Y. Differential
activation of Matrix metalloproteinases in heart
failure with and without ventiicular dilation.
Cardiovasc Res. 57: 766-774, 2003.
15.Peterson, J. T., Li, H. and Dillon, L. Ecolution of
Matrix metalloproteinase and tissue inhibitor during
heart failure progress-sion in infracted rat.
Cardiovasc. Res. 46: 307-315, 2000.
16.Pfeffer, M. A. and Braunwald, E. Ventricular remodeling
after myocardial infarction: experimental observatiobns
and clinical implications. Circulation81:1161-1172,
17.Piorlo, J. S., Hutchins, G. M. and Moore, G. W. Infarct
expansion: pathologic analysis of 204 patients with a
single myocardial infarct. J. Am. Coll Cardiol. 7: 349-
18.Romanic, A. M., Burns-kurtis, C. I. and Gout, B. Matrix
metalloproteinase in cardiac myocytes follow myocardial
infarction in the rabbit . Life Sci. 68: 799-814, 2001.

19.Rosemberg, G. A., Navartil, M, Barone, F. and
Feuerstein, G. Proteolytic cascade enzymes increase in
focal cerebral ischemia in rat. J. cereb. Blood flow
Metab. 16: 360-366, 1996.
20.Sato, S., Ashraf, M. and Millard, R. W. Connective
tissue changes in earlyinchemia of porcine
myocardium:an ultrasuructural study. J. Mol. Cell
Cardial. 15: 261-275, 1983.
21.Schwartzkopff, B., Fassbach, M., Pelzer, B., Brehm, M.
and Strauer, B. E. Elevated serum markers of collagen
degradation in patients with mild to moderate dilated
cardiomyopathy . Eur. J. Heart Fail. 4: 439-445, 2002.

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1. 12、杜政榮,2005,「台灣濕地環境之永續管理」,生活科學學報,第9期,第93-114頁。
2. 28、夏禹九,黃良鑫,王立志,黃正良,1992,「不同森林植被之淨輻射能與日輻射能關係」, 林業試驗所研究報告季刊,第7卷,第4期,第371-374頁。
3. 30、徐森雄,陳蕙蕙,沈立文,陳六合,1999,「從微氣象資料推估奧古斯丁草地之蒸發量」,中華農業氣象,第6卷,第2期,第105-112頁。
4. 31、徐森雄,黃國禎,王少峰,1991,「從微氣象資料推測盤固牧草地之蒸發散量」,中華農學會報,第154期,第82-93頁。
5. 47、郭勝豐,程澄元,劉振宇,2006,「倒傳遞神經網路應用於嘉南灌區作物蒸發散量之推估」,農業工程學報,第52卷,第1期,第24-34頁。
6. 48、陳世偉,吳俊毅,高志明,張有義,2006,「高屏溪舊鐵橋人工濕地水質淨化功能探討:一個親水的自然系統」,中華民國環境保護學會學刊,第2卷,第29期,第14-30頁。
7. 57、陸象豫,劉瓊霦,黃良鑫,唐凱軍,2002,「坡地檳榔園對微氣候影響之研究」,臺灣林業科學,第17卷,第2期,第281-289頁。
8. 59、童慶斌,連宛渝,2000,「氣候變遷對灌溉需水量的影響」,中國農業工程學報,第46卷,第1期,第57-68頁。
9. 60、黃成輝,曾偉君,2006,「台灣沿海濕地以及紅樹林之遊憩效益」,觀光研究學報,第12卷,第1期,第43-66頁。
10. 62、黃振昌,宋易倫,2004,「不同計量期距的Penman-Monteith方程式日射-日照關係地域性參數之探討」,農業工程學報,第50卷,第4期,第77-92頁。
11. 64、葉信富,李振誥,陳忠偉,張格綸,2008,「評估蒸發皿係數以推估台灣南部地區蒸發散量之研究」,農業工程學報,第54卷,第3期,第27-35頁。
12. 65、蔡西銘,邱金火,林殷瑋,2001,「地理資訊系統推估區域蒸發散量之研究」,中原學報,第29卷,第3期,第219-229頁。