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研究生:甘鎮豪
研究生(外文):Chen-Hao
論文名稱:香蕉皮正己烷萃取物之類胡蘿蔔素組成及其生物活性
論文名稱(外文):Carotenoids and Biological activities from n-hexane extracted of banana peel
指導教授:王進崑王進崑引用關係陳肅霖陳肅霖引用關係
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:營養學研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:90
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香蕉為是全世界消費量最大的水果。香蕉有促進消化、抗憂鬱、抗氧化、抗癌等功能,香蕉經成熟後果皮的顏色會轉黃,可能含有多量的類胡蘿蔔素。因此本研究除測定香蕉皮中類胡蘿蔔素的種類及含量,並測定其生物活性。本研究取用台灣產北蕉的果皮及果肉,分別取低極性正己烷(n-hexane)萃取物、中極性乙酸乙酯(ethylacetate)(EA) 萃取物、高極性水(water) 萃取物進行生物活性之探討。結果顯示,香蕉皮的抗氧化性明顯優於香蕉肉,而香蕉皮低級性萃取物具有最佳之抗氧化性與清除自由基的能力。 香蕉皮低極性萃取物中含有α-carotene、ß-carotene、lutein等類胡蘿蔔素,其中又以lutein的含量最多(0.52mg/g dry wt.),以藍光(430±30nm)照射 ARPE-19細胞,造成細胞損傷之模式顯示,香蕉皮之低極性萃取物具有明顯保護細胞並使細胞增生的作用。 此外,香蕉皮低極性萃取物能夠有效抑制KB及ARPE-19細胞cyclooxygenase 2之表現,並且可促進KB細胞發生凋亡的現象。

Banana is greatly consumed in the world. Banana shows digestive promotion, anti-depression, anti- oxidation, and anticancer, Mature banana peel contains rich carotenoids, This study was to determine the carotenoids of the banana peel, and also its biological activities.
Taiwan Pei Chial was used in this study Low-polar (n-hexane),semi-polar (ethylacetate) and high-polar (water) extracts were obtained for discussing the biological activities. Results showed that banana peel had better antioxidation than that of pulp. Low-polar (n-hexane) extract of banana peel showed perfect antioxidation and scavenging efficiency for free radicals. Low-polar extract of banana peel included α-carotene ß-carotene and lutein, and the levels of leutin were very high. In blue light exposure MTT assay test, blue light (430nm±30) could destroy ARPE-19 cell (human retinal pigment epithelial cell line). Low-polar extract of banana peel extract could restore the damage of ARPE-19 cell.
In addition, Low-polar extract of banana peel inhibited the expression of COX-2 of KB cell and ARPE-19 cell. Low-polar extract of banana peel also induced KB cell apoptosis.


目錄
頁次
表次.............................................Ⅰ
圖次....................................................Ⅱ
附錄....................................................Ⅳ
中文摘要................................................Ⅴ
英文摘要................................................Ⅵ
壹、前言.................................................1
貳、文獻整理.............................................2
一、香蕉簡介............................................2
(一)、香蕉的歷史.......................................2
(二)、香蕉的分類.......................................2
(三)、香蕉的組成份.....................................4
(四)、香蕉的生理功能...................................7
二、類胡蘿蔔素..........................................9
(一)、類胡蘿蔔素的簡介.................................9
(二)、類胡蘿蔔素的特性.................................12
(三)、類胡蘿蔔素的應用.................................12
(四)、香蕉中的類胡蘿蔔素...............................16
三、發炎反應...........................................16
四、細胞週期...........................................20
五、細胞凋亡...........................................23
參、研究目的.............................................25
肆、試驗設計.............................................26
伍、材料與方法.............................................27
一、試驗材料............................................27
(一)、香蕉.............................................27
(二)、香蕉樣品處理流程.................................27
(三)、香蕉皮與香蕉肉不同極性萃取物製備.................28
二、試驗用藥............................................29
三、試驗儀器............................................32
四、試驗方法............................................35
(一)、香蕉清除自由基能力測定...........................35
1.DPPH自由基能力清除..................................35
2.超氧陰離子之清除作用................................35
(二)、香蕉皮類胡蘿蔔素含量測定.........................36
(三)、細胞培養.........................................36
1.PBS製備.............................................36
2.DMEM培養液的製備....................................37
3.DMEM-F12培養液的製備................................37
4.冷凍細胞活化........................................37
5.細胞株培養..........................................38
6.細胞冷凍保存........................................38
7.細胞計數............................................38
8.細胞存活試驗 (MTT assay)............................39
(四)、眼球上皮細胞(ARPE-19)藍光照射試驗................40
(五)、細胞凋亡試驗.....................................40
(六)、第二型環氧化酶(COX-2)活性測定....................42
1.細胞內蛋白質萃取....................................42
2.西方墨點法 (Western blotting assay).................43
五、統計分析.............................................45
陸、結果與討論...........................................46
一、抗氧化性............................................46
1.DPPH自由基能力清除..................................46
2.超氧陰離子之清除作用................................46
二、香蕉皮類胡蘿蔔素化合物之分析........................48
三、對眼球上皮細胞(ARPE-19)存活率之影響.................53
四、對人類口腔癌細胞(KB cell)存活率之影響...............57
五、細胞凋亡試驗........................................57
六、對人類眼球上皮細胞中COX-2表現影響...................61
七、對人類口腔癌細胞中COX-2表現影響.....................61
柒、結論.................................................66
捌、參考文獻.............................................67
玖、附錄.................................................78




王宇、于孝軍、李劍紅、崔衛國:多巴胺及其受體的發展。黑龍江畜牧獸醫專刊。9:67-68 (2003)
台灣地區食品營養份資料庫(行政院衛生署食品衛生處)http://www.doh.gov.tw/newdoh/90-org/org-3/database/4-1.html (2004)
台灣香蕉研究所 http://www.banana.org.tw/download.asp
江伯源:台灣水果的風雲代表。農業世界雜誌。177:79-83 (1998)
李世凔:南國嬌客香蕉。農業世界雜誌。224:34-39(2002)
吳秋燁:蜂膠不同溶劑萃取物消化前後組成及生物活性之評估。中山醫學大學營養科學研究所 碩士論文(2003)。
許振耀:著色劑。食品添加物使用法。113-115 (1979)。
陳一郎:利用化學電極偵測多巴胺及五基色胺酸之研究。國立中興大學化學系研究碩士論文。台中市。(1997)
陳炳輝:類胡蘿蔔素的特性與應用。科學發展月刊。28(8):599-604 (2000)
陳惠英、顏國欽:類胡蘿蔔素之生理功能。食品工業。26(11):19-31 (1994)
國家網路藥典 http://www.webhospital.org.tw/medicine/ (2005)
張鈺驩:基礎食品化學。藝軒圖書出版社。192-194,236-239 (1990)
蔣世超:香蕉的生化特性營養價值與加工產品。農業世界雜誌。188:66-70 (1999)。
顏麗姝:胡蘿蔔素分析方法之簡介。食品工業。29(9):33-43(1997)
鄧澄欣:台灣香蕉栽培種特性介紹。農業世界雜誌。224:25-33 (2002)
龍雲、周旭光、馮建章、童沈陽:食品中多巴胺測定方法研究。北京大學學報。30:425-428 (1994)
Alanko J., Riutta A., Vapaatalo H. (1992). Effect of catecholamines on eicosanoid synthesis with special reference to prostanoid/leukotriene ratio. Free Radical. Biol. Med. 13, 677-688.
Allen DR., Wallis GL., McCay PB. (1994). Catechol adrenergic agents enhance hydroxyl radical generation in xanthine oxidase systems containing ferritin : Implications for ischemia/reperfusion. Arch Biochem. Biophys. 315 : 235-245.
Axelrod J., Weinshilboum R. (1972). Catecholamines. N Engl. J. Med. 287 : 237-242.
Badria FA. (2002). Melatonin, serotonin, and tryptamine in some egyptian food and medicinal plants. J Med Food. Fall;5(3):153-7.
Bailey CA., Chen BH. (1998). Carotene and xanthophylls change during growth and processing of turf bermudagrass. Poultry Sci 67:1644.
Bauernfeind JC. (1972). Carotenoid vitamin A precursors and analogs in foods and feeds. J Agric Food Chem.43:2376-2878.
Bauernfeind JC. (1981). Carotenoids as colorants and vitamin A precursors. Food Sci Technol 883.
Barry H., John G. (1998). Free radicals in biology and medicine. Oxford Univery Press.
Burton GW., Ingold KU. (1984). β-carotene:an unsual typeof liquid Antioxidant . Sci 224:569-573.
Botting KJ., Young MM., Pearson AE., Harris PJ., Ferguson LR. (1999). Antimutagens in food plants eaten by Polynesians: micronutrients, phytochemicals and protection against bacterial mutagenicity of the heterocyclic amine 2-amino-3-methylimidazo[4,5-f]quinoline. Food Chem Toxicol 37(2-3), 95-103.
Bros W., Heller W., Michel C., Saran M. (1990). Flavonoids as antioxidants : determination of radical-scavenging efficiencies. Methods Enzymol. 186, 343-355.
Cadet JL., Brannock C. (1998). Free radicals and the pathobiology of brain dopamine systems. Neurochem. Int 32, 117-131.
Campbell NA., Reece JB. (2002). The cell cycle. In: Biology (Campbell, N. A.and Reece, J. B. eds.), pp: 226. Benjamin Cummings, San Francisco.
Carnevale J., Cole ER., Crank G. (1979). Fluorescent light catalyzed autoxidation of b-carotene.J Agric Food Chem 27(2), 462-463.
Chen BH., Chen. (1992). Studies on the stability of carotenoids in garland chrysanthemum as affected by microwave and conventional heating.J Food Proc 55, 296-300.
Chen BH., Chen CS., Hwang LS. (1993). Effect of different drying methods on the formation of carotenoid artifacts in turf Bermuda grass.J Chinese Agric Chem Soc31(5), 642-652.
Chen BH., Chen TM., Chien JT. (1994). Kinetic model for studying isomerization of a and b-carotene during heating and illumination.J Agric Food Chem 42, 2391-2397
Chen BH., Han LH. (1990). Effect of different cooking methods on the yield of carotenoids in water convolvulus (Ipomoea aquatica)).J Agric Food Proc 53(12), 1076-1078
Cheng HC., Chien H., Liao CH., Yang YY., Huang SY. ( 2007). Carotenoids suppress proliferating cell nuclear antigen and cyclin D(1) expression in oral carcinogenic models J Nutr Biochem. Mar 16.
Chou HE., Breene WM. (1972). Oxidative decoloration of β-carotene in low-moisture model system.J Food Sci 37, 66-68.
Cooper J., Bloom F., Roth R. (1996). The Biochemcial Basis of Neurosychopharmacology. New York : Oxford Univ Press. pp. 54
Davey MW., Keulemans J., Swennen R. (2006). Methods for the efficient quantification of fruit provitamin A contents.J Chromatogr A. Dec 15;1136 (2), 176-84.
Davies BH. (1976). Carotenoids,Ch.19,In”Chemistry and Bilchemistry of Plant pigments..Vol 2”,T.W.Goodwin(Ed.),Academic Press,New York.
De Mejia EG., Prisecaru VI. (2005). Lectins as bioactive plant proteins: a potential in cancer treatment. Critical Reviews in Food Science & Nutrition 45(6), 425-45.
Edge R., McGarvey DJ., Truscott TG. (1997). The carotenoids as anti-oxidants-a review.J Photochem Photobiol B. 41(3), 189-200.
Englberger L., Marks GC., Fitzgerald MH. (2004). Factors to consider in Micronesian food-based interventions: a case study of preventing vitamin A deficiency Public Health Nutrition. 7(3), 423-31.
Feldman JM., Lee EM. (1985). Serotonin content of foods: effect on urinary excretion of 5-hydroxyindoleacetic acid. Am J Clin Nutr.;42(4), 639-43.
Garcia-Moreno C., Nogales-Alarcon A., Gomez-cerro A., Marine-Font A. (1980). Spectrofluorometric determination and thin layer chromatographic identification of serotonin in foods. J Assoc Off Anal Chem.; 63(1), 19-21.
Godoy HT., Roderiguez-Amaya DB. (1989). Carotenoid composition of commercial mangoes form Brazil. Lebensm-Wiss Technol 22(3), 100-103.
Goel RK., Gupta S., Shankar R., Sanyal AK. (1986). Anti-ulcerogenic effect of banana powder (Musa sapientum var. paradisiaca) and its effect on mucosal resistance. Journal of Ethnopharmacology. 18(1), 33-44.
Goodwin TW. (1986). Metabolism,nutrition,and function of carotenoids. Ann Rev Nutr 6, 273-297.
Gorge BK. (1965). The pharmacological Basis of Therapeutics, 3 rd ed. The Macmillan Company. New York. Pp. 427.
Grace A., Bunney B. : Electtrophysiologyical properties of midbrain dopamine neurons. In : F Bloom and D Kupfer Eds.
Green, DR., Reed, JC. (1998). Mitochondria and apoptosis. Science 281,1309-1312.
Gregory GK., Chen T., Philip T. (1986). Quantitative analysis of lutein esters in marigold flowers by high performance liquid chromatography. J Food Sci 51(4), 1093.
Gupta PK. (2000). Signal transduction in the nervous system : The 2000 Nobel Prize for physiology or medicine [J]. Current Science. 79 : 9-11.
Handelman GJ. (2001). The evolving role of carotenoids in human biochemistry. Nutr 17, 818-822.
Haralampu SG., Karel M. (1983). Kinetic models for moisture dependence of ascorbic acid and b-carotene degradation in dehydration in dehydrate sweet potato.J Food Sci 48, 1872-1873.
Helander A., Some M. (2000). Dietary serotonin and alcohol combined may provoke adverse physiological symptoms due to 5-hydroxytryptophol. Life Sci. Jul 7;67(7), 799-806.
Helander A., Wikstrom T., Lowenmo C., Jacobsson G., Beck O. (1992). Urinary excretion of 5-hydroxyindole-3-acetic acid and 5-hydroxytryptophol after oral loading with serotonin. Life Sci:50(17), 1207-13.
Henry LK., Catignani GL., Schwartz SJ.(1998). Oxidative degradation kinetics of lycopene, lutein, and 9-cis and all trans β-carotene. J. AOAC. 75, 823-829.
Hetts SW. (1998). To die or not to die: an overview of apoptosis and its role in disease. JAMA 279, 300-307.
Hills BA., Kirwood CA. (1989). Surfactant approach to the gastric mucosal barrier: protection of rats by banana even when acidified. [Journal Article] Gastroenterology. 97(2), 294-303.
Junghans A., Sies, H., Stahl W. (2001). Macular pigments lutein and zeaxanthin as blue light filters stddied in liposome. Arch. Biochem. Biophys. 391, 160-164.
Kanazawa K., Sakakibara H. (2000). High content of dopamine, a strong antioxidant, in Cavendish banana. J. Agric. Food Chem. 48, 844-848.
Kellim JA., Decker JM. (2001). Use of dopamine in acute renal failure : A metaanalysis. Crit Care Med. 29(8), 1526-1531.
Kelly RB. (1993). Storage and release of neurotransmitters Cell. 10, 43-53.
King MW. (2003). Tyrosine-Derived Neurotransmitters. Indiana State University. http://web.indstate.edu/thcme/mwking/aminoacidderivatives.html .
Kotake-Nara E., Kushiro M., Zhang H.,Sugawara T., Miyashita K., Nagao A. (2001). Carotenoids affects proliferation of human prostate cancer cells.J Nutr 131, 3303-3306.
Koshte VL., Aalbers M., Calkhoven PG., Aalberse RC. (1992). The potent IgG4-inducing antigen in banana is a mannose-binding lectin, BanLec-I. International Archives of Allergy & Immunology. 97(1), 17-24.
Koyama Y., Hosomi M., Miyata A., Hashimoto H., Reames SA. (1988). Supplementary and revised assignment of the peaks of the 7,9-,9,9-13,13-,9,13-di-cis and 9,9,13-tri-cis isomers of β-carotene in high-performances liquid chromatography using a column of calcium hydroxide.J Chromatoger 439, 417-422.
Kuchel O., Buu NT., Serri O. ( 1982). Sulfoconjugation of catecholamines, nutrition, and hypertension. Hypertension. 4(5 Pt 2), III93-8.
Landrim, JT., Bone RA. (2001). Lutein, zeathanthin, and the macular pigment. Archi. Biochemi. Biophys. 385, 28-40.
Lee WG., Ammerman GR. (1974). Carotene stereoisomerization in sweet potatos as affect by rotating and still retort and still retort canning processes,J Food Sci 39, 1188-1190.
Lee SY., Choi KY., Kim MK., Kim KM., Lee JH. Meng KH., Lee WC. (2005). The relationship between intake of vegetables and fruits and colorectal adenoma-carcinoma sequence. Korean J Gastroenterol. 45(1), 23-33.
Liaaen-Jensen S.(1971). Isolation, reactions In ”carotenoids”. Ed. Isler, O. pp. 61-188.
Liebler DC., McClure TD.(1996). Antioxidant reaction of β-carotene:identification of carotenoid-radical adducts.Chem Res Toxicol 9, 8-11.
Liu J., Mori A. (1993). Monoamine metabolism provides an antioxidant defense in the brain against oxidant and free radical induced damage. Arch. Biochem. Biophys. 302, 118-127.
Lukiw WJ., Mukherjee PK., Cui JG., Bazan NG. (2006). A2E selectively induces cox-2 in ARPE-19 and human neural cells. Curr Eye Res. 31(3), 259-63.
Luo Y., Umegaki H., Wang X., Abe R., Roth GS. (1998). Dopamine inducesapotosis through an oxidation-involved SAPK/JNK activation pathway. J. Biol. Chem. 273, 3756-3764.
Lohsoonthorn P., Danvivat D. (1995). Colorectal cancer risk factors: a case-control study in Bangkok. Asia-Pacific Journal of Public Health. 8(2), 118-22.
MacNaughton WK., Wallace JL . (1989). A role for dopamine as an endogenous protective factor in the rat stomach. Gastroenterology. 96, 972-980.
Mezey E. (1998). Dopamine produced by stomach may act as a pacracrine/autocrine hormone in the rat [J] . Neuroendocrinology. 67(5), 336.
Miller NJ.,Sampson J., Candeias LP., Bramley PM., Rice-Evans CA. (1996). Antioxidant activities of carotene and xanthophylls. FEBS Lett 384, 240-242.
Miura T., Muraoka S., Ogiso T. (1996). Lipid per oxidation inhibited by monoamines. Res. Commun. Mo.l Pathol. Pharmacol. 93, 57-67.
Nagaraja P., Srinivasa KC., Murthy KS., Rangappa NM. (1998). Made gowda spctrophotometric methods for the determination of certain catecholamine detervatives in pharmaceutical preparations. Talanta, 39-44.
O’Neil CA., Schwartz SJ. (1992). Chromatographic analysis of cis/trans carotenoid isomers. J Chromatogr 624, 235-252.
Ottaway PB. (1993). The technology of vitamins in food.Blackie Academic and professional,Chapman and hall.Cornewall,England, 21-185.
Pannangpetch P., Vuttivirojana A., Kularbkaew C., Tesana S., Kongyingyoes B., Kukongviriyapan V. (2001). The antiulcerative effect of Thai Musa species in rats. Phytotherapy Research. 15(5), 407-10.
Paolini M., Abdel-Rahman SZ., Sapone A., Pedulli GF., Perocco P., Cantelli-Forti G., Legator MS. (2003). ß-carotene:a cancer chemopreventive agent or a co-carcinogen?. Mutat Res 543, 195-200.
Pesek CA.,Warthesen JJ. (1988). Characterization of the photodegration of b-carotene in aqueous model system. J Food Sci 53(5), 1517-1520.
Pesek CA., Warthesen JJ., Taoukis PS. (1990). A kinetic model for equilibration of isomeric ß-carotene in food materials. J Agric Food Chem 38, 41-45.
Philip T., Chen TS. (1988). Separation and quantitative analysis of some carotenoid fatty acid esters of fruits by liquid chromatography. J Chromatogr. 1;435(1), 113-26.
Posadas I., Terencio MC., Guillen I., Ferrandiz ML. (2000). Coregulation between cyclogenase-2 and inducible nitric oxide synthase expression in the time-course of murine inflammation. Naunyn-Schmiedeberg''s Arch Pharmacol 361, 98-106.
Rafi MM., Shafaie Y.( 2007). Dietary lutein modulates inducible nitric oxide synthase (iNOS) gene and protein expression in mouse macrophage cells (RAW 264.7). Mol Nutr Food Res. 51(3), 333-40.
Ralph RM., McCarthy MJ., Kissinger PT. (1976). Identification of salsolinol as a major dopamine metabolite in the banana. J. Agric. Food Chem. 24, 198-191.
Rapp PR., Amaral DG. (1992). Individual difference in the cognitive and neurobiological consequence of normal aging . Trends Neurosci. 15, 340-345.
Ray A., Henke PG., Sullivan R.M.(1987). The central amygdale and immobilixation stress-induce gastric pathology in rats : Neurotensin and dopamine. Brain Res. 409(2), 398.
Ribaya-Mercado JD., Garmyn M., Gilchrest BA., Russell RM. (1995). Skin lycopene is destroyed preferentially over beta-carotene during ultraviolet irradiation in humans. J Nutr. 125(7), 1854-9.
Robak, Jadwiga and Gryglewski RJ. (1998). Flavonoids are scavengers of superoxide anions. Biochem. Pharmacol. 37(5), 837-841.
Rodriguez-Amaya DB. (1989). Critical review of provitamin A determination in plant foods. J Micronutrient Anal 5, 191-225.
Saguy I., Goldman M., Karel M. (1985). Prediction of ß-carotene decoloration in model system under static and dyamic conditions of reduced oxygen environment. J Food Sci 50, 526-530.
Saleh MH., Tan B. (1991). Separation and identification of cis/trans carotenoid isomers. J Agric Food Sci 39, 1438.
Sellers WR., Fisher DE. (1999). Apoptosis and cancer drug targeting. J Clin Invest 104, 1655-1661.
Shah MA., Schwartz GK. (2001). Cell cycle-mediated drug resistance: an emerging concept in cancer therapy. Clin. Cancer Res. 7, 2168-2181.
Sharma SK., Maguer ML. (1996). Kinetics of lycopene degration in tomatopulp solids under different processing and storage conditions. Food ResInt 29(3), 309-315.
Smith ML. Fornace AJ., Jr. (1996). Mammalian DNA damage-inducible genes associated with growth arrest and apoptosis. Mutat. Res. 340, 109-124.
Smith WL., DeWit DL., Garavito RM. (2000). Cyclooxygenase: structural, cellular, and molecular biology. Annu Rev Biochem 69, 145-182.
Sparrow JR., Zhou J, Ben-Shabat S., Vollmer H., Itagaki Y., Nakanishi K.,( 2002). Involvement of oxidative mechanisms in blue-light-induced damage to A2E-laden RPE., Invest Ophthalmol Vis Sci.43(4), 1222-7.
Stamatakis K., Francisco JS.(2004) Protein modification by cyclopentenone prostaglandin addition: biological actions and therapeutic implications. Gene Ther Mol Biol Vol 8, 241-258
Subagio A., Morita N., Sawada S. (1996). Carotenoids and their fatty-acid esters in banana peel. J Nutr Sci Vitaminol 42(6), 553-66.
Sun J., Chu YF., Wu X., Liu RH. (2002). Antioxidant and antiproliferative activities of common fruits. [Journal Article] Journal of Agricultural & Food Chemistry. 50(25), 7449-54.
Takashashi Y., Sasaki S., Takashashi M., Okubo S., Hayashi M., Tsugane SA. (2003). population-based dietary intervention trial in a high-risk area for stomach cancer and stroke: changesin intakes and related biomarkers. Prev Med 37, 432-441.
Taylor A., Hobbs M. (2001). Assessment of nutritional influences on risk for cataract. Nutr 17(10), 845-857.
Tee ES. (1992). Carotenoids and retinoids in human nutrition. Crit Rev Food Sci Nutr 31(1/2), 103-163.
Tee Es., Lim CL. (1991). Carotenoid composition and content of Malaysian vegetables and fruits by AOAC and HPLC methods. Food Chem 41, 309-339.
Telford WG., King LE., Fraker PJ. ( 1994). Rapid quantitation of apoptosis in pure and heterogeneous cell populations using flow cytometry. Journal of Immunological Method. 172, 1-16.
Tetsuka T., Baier LD., Morrison AR. (1996). Antioxidants inhibit interleukin-1-induced cyclooxygenase and nitric oxide synthase expression in rat mesangial. J Biol Chem 271, 11689-11693.
Tsukida K, Saiki K, Takii T, Koyama Y. (1982). Separation and determination of cis/trans-β-carotene by high-performance liquid chromatography. J Chromatography.J Chromatogr 245, 359-364.
Udenfriend S., Lovenberg W., Sjoerdsma A. (1959). Physiologically active amines in common fruits and vegetables. Arch. Biochem. Biophys. 85 , 487-490.
Velasco M., Luchsinger A. (1998). Dopamine : Pharmacologic and therapeutic aspects. Am. J . Ther . 5(1), 37-43.
Viktorsson K., De Petris L. Lewensohn R. (2005). The role of p53 in treatmentresponses of lung cancer. Biochem. Biophys. Res. Commun. 331, 868-880.
Volkow ND., Wang G., Gur RC. (1998). Association between decline in brain dopamine activity with age and cognitive and motor impairment in healthy individual. Am. J. Psychiatry. 155, 344-349.
Williams AW., Boileau TW., Zhou JR., Clinton SK., Erdman Jr JW.(2000). Beta-carotene modulates human prostate cancer cell growth and may undergo intracellular metabolism to retinol. J Nutr 130, 728-732.
Willis MS., Wians Jr FH. (2003). The role of nutrition in preventing prostate cancer: a review of the proposed mechanism of action various dietary substances. Clin Chim Acta 330, 57-83.
William, RB., Michael WP. (1994). Antioxidand nutrients and protection from free radicals. Nutr Toxicol, 19-48.
Yeh CC., Hsieh LL., Tang R., Chang-Chieh CR., Sung FC. (2005). Vegetable/fruit, smoking, glutathione S-transferase polymorphisms and risk for colorectal cancer in Taiwan.World J Gastroenterol. 14;11(10), 1473-80.
Zechmeister L. (1994). Cis-trans isomerization and stereo chemistry of carotenoids and diphenyl-polyenes.Chem Rev34, 267-322.


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