跳到主要內容

臺灣博碩士論文加值系統

(44.211.26.178) 您好!臺灣時間:2024/06/16 01:21
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:蔡燕真
研究生(外文):Yan-Jhen
論文名稱:菊糖於STZ誘發之小白鼠糖尿病之影響
論文名稱(外文):Effects of Inulin in STZ-induced Diabetes mice
指導教授:徐成金徐成金引用關係
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:營養學研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:74
相關次數:
  • 被引用被引用:0
  • 點閱點閱:1415
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
糖尿病已為台灣地區最重要的慢性病之一,罹患率高居國人十大死因之一。糖尿病所引起的併發症為病患死亡的主要原因,糖尿病併發症的發生與糖尿病所導致的血糖過高(hyperglycemia)有密切相關。在體內過高的血糖濃度,細胞內的葡萄糖會經由多元醇(polyol pathway)來進行代謝,許多文獻指出此代謝路徑會導致糖尿病併發症-視網膜病變、神經病變以及心血管疾病之發生,再者糖尿病者常伴隨著脂質代謝異常,高血糖的氧化壓力是誘發血管病變的關鍵因子,因此良好的血糖控制對於糖尿病患減緩併發症的發生是很重要的。
本研究探討菊糖於streptozotocin (STZ)誘發小鼠糖尿病模式之影響。使用BALB/c 雄鼠經由尾靜脈注射STZ (200mg/kg BW)誘發糖尿病,菊醣是一種含有大量的果寡糖的天然物,而果寡糖具有膳食纖維特性,因此本實驗目的在探討菊糖添加入飼料中,對STZ誘發之糖尿病小鼠體內脂質及醣類代謝之影響。實驗共進行四週,分為四組(1)Control組 (2)Diabetes組 (3) Diabetes+inulin組 (4) Control+inulin 組,實驗期間給予每隻小鼠每日5克飲食攝取量並添加濃度11%的菊醣及自由飲水。
結果顯示,食用添加菊糖的糖尿病鼠,降低了血漿及肝臟中醛糖還原酶、乙二醛酶的活性與肝臟中山梨醇脫氫酶的活性,而血漿中的葡萄糖、三酸甘油脂、膽固醇及纖維網狀蛋白的量都有明顯下降,在抗氧化作用的部分,食用添加菊糖的糖尿病鼠,血漿及肝臟中丙二醛值有顯著下降,而麩胱酐肽氧化酶濃度則顯著上升。上述實驗結果顯示菊糖應具有預防或降低糖尿病併發症發生的效果。


Diabetes is one of the most important chronic diseases in Taiwan. The complications from diabetes mellitus are the main causes responsible for the death of patients. Hyperglycemia occurred in diabetes results in oxidative and glycative stress from polyol pathway. Many papers have indicated that both oxidation and glycation contribute to the development of diabetic neuropathy, diabetic nephropathy and cardiovascular disease. Thus, any agents with anti-oxidant and/or anti-glycative effect may retard the presence of diabetic complications.
Inulin is a natural compound with fiber-like property. In our present study, streptozotocin (STZ) was used to induce diabetes in Balb/c mice, and inulin was supplied. The purpose of this study was to investigate the effect of this compound on anti-oxidant protection and lipid metabolism in diabetic animal model. Results showed that the intake of inulin decreased the activity of aldose reductase, glyoxalase I and sorbitol dehydrogenase in the plasma and liver. Inulin intake also increased glutathione peroxidase activity, reduced lipid oxidation level and decreased the level of blood glucose, triglyceride, cholesterol and fibronectin in diabetic mice. These results suggested that inulin might provide alleviative effects against diabetic deterioration.


目 次

目次...........................................................................................................2
中文摘要....................................................................................................7
英文摘要....................................................................................................8
第一章 前言…………………………………………………………......10
第二章 文獻探討......................................................................................11
一、糖尿病........................................................................................11
二、高血糖與多元醇路徑之相關酵素……………………………13
三、高血糖與糖化中產物................................................................15
四、糖尿病併發症……....................................................................18
五、菊糖……………………............................................................19
六、膳食纖維………………………………………………………20
七、糖尿病動物實驗模型................................................................23
第三章 研究目的......................................................................................25
第四章 實驗材料與方法......................................................................... 26
一、實驗材料....................................................................................26
二、實驗方法....................................................................................29
三、分析方法....................................................................................30
四、統計分析....................................................................................40
第五章 實驗結果......................................................................................41
一、 飲食中添加菊糖對STZ誘發糖尿病小白鼠之
動物模式症狀之影響...........................................………......41
二、 飲食中添加菊糖對STZ誘發糖尿病小白鼠血糖
之影響.....................................................................................41
三、 飲食中添加菊糖對STZ誘發糖尿病小白鼠血漿
中脂質之影響.........................................................................41
四、 飲食中添加菊糖對STZ誘發糖尿病小白鼠血漿
及腎臟中脂質氧化作用之影響.............................................42
五、 飲食中添加菊糖對STZ誘發糖尿病小白鼠血漿
及肝臟中麩胱酐肽氧化酶活性之影響.................................42
六、 飲食中添加菊糖對STZ誘發糖尿病小白鼠
血漿及肝臟中醛酸還原酶之影響.........................................42
七、 飲食中添加菊糖對STZ誘發糖尿病小白鼠血漿
及肝臟中乙二醛酶活性之影響…………………………….42
八、 飲食中添加菊糖對STZ誘發糖尿病小白鼠肝臟
中山梨醇脫氫酶之影響.........................................................43
九、 飲食中添加菊糖對STZ誘發糖尿病小白鼠血漿
中纖維網狀蛋白之影響.........................................................43

第六章 討論.............................................................................................44
第七章 結論…………………………………………………………….48
第八章 參考文獻.....................................................................................62



1.Bingham.S.(1987) Definitions and intake of dietary fiber.Am.J.Clin.Nutr .45:1226-1231

2. Cummings.J.H.and Euglyst,H.N.(1991)Trends in food Science and Technology,April.99-103

3. Reberfroid ,M.(1993)Dietary fiber .inulin and oligofructose;a review comparing their physiological effect critical review in food.Sci and Nutr.33:103-148

4. Jenkins,D.J.A. Leeds,A.R,Newton,C and Cumming,J.H(1975)Effect of pectin gum and weat fiber on serum-cholesterol Lancet 1:1116-1117

5. Anderson ,J.W.and Akanji ,A.O.(1991)Dietary fiber- an overview. Diabetes Care. 14:1126-1131

6. Jensen ,C.D.Spiller,G.A.Gates ,J.E Miller ,A.F and Whittan ,J.H.(1993) The effect of acacia gum and a water soluble dietary fiber mixture on blood lipids in humans .J. Am.coll .Nutr.12:147-154

7. Ross, A.H.M.,Eastwood , M.A.,Brydon ,W.G,.Anderson ,J.R.and Anderson ,D.M.W.(1983) A study of the effects of dietary gum arabic in humans .Am.J.Clin .Nutr. 37:368-375

8. National Heart Lung and Blood Institutes ,National Institutes of Health (1985) Consensus Conference:Lowering blood cholesterol to prevent heart disease, J.Am.Med.Assoc. 253:2080-2086

9. Crouse ,J.R.,Gerson ,C.D.Oscarli ,L.M. and Liebers, C.S.(1968) Role of acetate in the reduction of plasma free fatty acids produced by ethanol in man .J.Lipid Res .9:509-513

10. Wolever ,T,M,S.,Brighenti ,E.,Royali ,D.,Jenkins .D.J.A(1989)Effect of rectal infusion of short chain fatty acids in human subjects .Am.J.Gastroenterol .84:1027-1032

11. Wolever ,T,M,S.,Spadafora ,P.and Eshuis ,H(1991)Interaction between colonic and propionate in humans .Am.J.Clin .Nutr.53:681-687

12. Scheeppach ,W., cummings J.H.Branch ,W.J. and Schrezenmeir,J.(1988a)Effect of gut-derived acetatue on oral glucose tolerance in man .Clin .Sci .75:355-361

13. Judson ,G.J.,Andreson ,E.and Luick ,J.R.(1968)The contribution of propionate to glucose synthesis in sheep given diets of different grain content. Br. J. Nutr. 2:69-75

14. Anderson, J . W .and Bridges, S. R.(1984) Short-chain fatty acid fermentation products of plant fiber affect glucose metabolism of isolated rat hepatocytes . Proc .Soc .Exp .Biol. Med. 177:372-376

15. Ross, A. H.M.,Eastwood, M. A., Brydon, W. G.,Anderson, D. M. W. (1983) A study of the effects of dietary gum arabic in humans. Am. J. Clin. Nutr. 37:368-375

16. Abraham, Z. D. and Mehta, T. (1988)Three- week psyllium-husk supplementation:effect on plassma cholesterol concentration, fecal steroid excretion, and carbohydrate absorption in men. Am. J. Clin. Nutr.47:67-74

17. Jensen, C. D,.Spiller, G. A.,Gates, J. E.,Miller, A. F. and Whittan, J.H.(1993)The effect of acacia gum and a water soluble dietary fiber mixture on blood lipids in humans. J.Am. Coll. Nutr. 12:147-154

18. Gambera, P.J.,Schnessman, B.O. and Davis, P. A.(1995)Use of the Food Guide Pyramind and USA Dietary Guidelines to improve dietary intake and reduce cardiovascular risk in active-duty Air Force members. J. Am.Diey.Assoc.95:1268-1273

19. Pick, M. E., Hawrysh, Z. J., Gee, M. L. and Hardin, R. T. (1996) Oat bran concentrate bread products improve long-term control of diabetes: A pilot study. J. Am. Diet. Assoc. 96: 1254-1261

20. Turley, S. D., Daggy, B. P. and Cietary, T. M. (1991) Cholesterol-lowering action of psyllium mucilliodin the hamster: sit and possible mechanism of action. Metabolism 40:1063-1073

21. Kritchevsky, D. nad Story, J. A. (1974) Binding of bile salts in vitro by non nutritive fiber. J. Nutr. 104:458-464

22. Thornton, R. and Heaton, W. (1981) Do colonic bacteria contribute to cholesterol gall-stone formation:effect of lactulose on bile. Br. Med. J. 282: 1018-1020

23. Lakesmanan, M. R., Nepokroeff, C. M., Nes, G. C., Dugan, R. E. and Porter, J. W. (1973) Stimulation by insulin of rat liver β-OH-β-methylglutery CoA reductase and cholesterol synthesizing activities. Biochem. Biophys. Res. Commun. 50: 704-710

24. Jenkins, D. J. A., Jenkins, A. L., Wolever, T. M. S., Vuksan, V. and Venken, A. (1995) Effect of reduced rate of carbohydrate absorption on carbohydrate and lipid metabolism. Eur. J. Clin. Nutr. 49(3): S68-S73
25. Cummings, J. H. (1981) Short chain fatty acid in the human colon. Gut 22: 763-779
26. Bridges, S. R., Anderson, J. W., Deakins, D. A., Dillon, D. W. and Wood, C. L. (1992) Oat bran increases serum acetate of hypercholesterolemic men. Am. J. Clin. Nutr. 56:455-459
27. Chen, W. J., Anderson, J. W. and Jennings, D. (1984) Propionate may mediate the hypocholesterolemic effects of certain soluble plant fibers in cholesterol-fed rats. Proc.Soc.Exp.Biol. Med.174: 21508-21516

28. Cheat, D. (1998) Animal model of type (insulin-dependent)diabetes
mellitus. J Pediatr Endocrinol Metab. 11: 11-19.

29. Saini, K. S., Thompson, C., Winterford, C. M., Waiker , N. I. &
Cameron , D. P. (1996) Streptozotocin at low dose induces apoptosis at high doses causes necrosis in a murine pancreatic bete cell line. Biochem Mol Biol Intern.39: 1229-1236.

30. Phares, C. K. (1980) Streptozotocin-induced diabetes in Syrian
hamsters : new model of diabetes mellitus. Experientia .36: 681-682.

31. Herr, R. R., Jahnke, H. K. & Argoudelis, A. D. (1967) The structure
of streptozotocin. J Am Chem Soc .89: 4809-4809.

32. Dakieten N, R. M., Nadkarni MV (1963) Studies on the diabetogenic
action of streptozotocin. Cancer Chemother.Rep. 29: 91-98.

33. Carter SK, B. L. a. F. M. (1971) Streptozotocin and metastatic
insulinoma. Ann.Intern.Med 74.

34. Wilson, G. L., Hartig, P. C., Patton, N. J. & LeDoux, S. P. (1988)
Mechanisms of nitrosourea-unduced β-cell damage .Activation of poly(ADP-ribose)synthetase and cellular distribution. Diabetes .37: 213-216.

35. Sato, Y., Hotta, N., Sakamoto, N., Matsupka , S., Ohishi, N. & Tagi,
K. (1979) Lipid peroxide level on plasma of diabetic patients. Biochem Med .21: 104-107.

36. Cooperstein, S.J., Watkins, D. & Jeanrenaud, B. (1980) Insulin
resistance in soleus muscle from obese Zucker rats. Biochem .J. 186: 525-534.

37. Junod, A., Lambert, E.A. & Renold, E.A. (1969) Diabetogenic action
of streptozotocin : Relationship of dose to metabolic response. J. Clin. Invest. 48: 2129-2139.

38. 郭錦松 & 蔡世澤 (1998) 糖尿病之診斷及分類的最新標準,臨床醫學42:1=247: 31-36。

39. 何菁菁。(2000) 糙薏仁對STZ所誘發之糖尿病大白鼠脂質與糖類代謝的影響。國立海洋大學 食品科學研究所碩士論文。

40. 蔡瓊滿。(2004) 大蒜水溶性含硫化合物於STZ誘發之糖尿病小鼠抗纖維化作用之影響。中山醫學大學營養科學研究所論文。

41. Kurup, S. & Bhonde, R. R. (2000) Combined effect of nicotinamide
and streptozotocin on diabetic status in partially pancreatectomized adult BALB/c mice. Horm Metab Res. 32: 330-334.

42.SAS Institiute Inc.(1990) SAS/STAT User,s Guide, version 6.SAS Institute, Cary, NC.

43.Ballard, D. J.,Mwlton, L. J.,3rd , Dwyer, M. S., Trautmann, J.C., Chu, C. P.,O''Fallom, W. M. & Palumbo, P. J. (1996)Risk factors for diabetic retinopathy:a population-based study in Rochester, Minnesota. Diabetes Care. 9:334-342

44. 郭清輝 (1998) 糖尿病慢性併發症. 臨床醫學 41:336-348

45. 曾慶孝 (2000) 糖尿病的大血管病變,中華民國內分泌暨糖尿病學會會訊 13:2

46. 陳國群編著 (1997) 最新糖尿病精要. 藝軒圖書出版社 第一版:118, 176

47. 呂榮國 (2002) 糖尿病神經病變. 當代醫學 29:819-824

48. 鄭文儀 & 莊立民(1999) 糖尿病神經病變. 當代醫學 26:51-52

49. Ohnishi, S. T. & Barr, J. K. (1978) A simplified method quantitating
protein using the biuret and phenol reagents. Analytical Biochemistry .86: 193
50. Ruoslahti, E., Hayman, E. G., Pierschbacher, M. & Engvall, E. (1982)
Fibronectin: purification, immunochemical properties, and biological activities. Methods Enzymol .82: 803-831.

51.Fraga, C.G.,B.E.Leibovitz,and A.L.Tapple,Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices:characterization and comparison with homogenates and microsomes .Free Radic Biol Med.1988.4(3):155-160

52.Lawrence,R.A.and R.F.Burk,Glutathion peroxidase activity in selenium-
deficientrat liver .Biochem Biophys Res Commun .1976.71(4):952-958

53.Animesh Chandra, Sanjay Srivastava, J.Mark Petrash, Aruni Bhatnagar,
Satish K. Srivastava(1997)Active site modification of aldose reductase by nitric
oxide donors .Biochimica et Biophysica Acta .1341:217-222

54.Franky Van Herreweghe, Jianqiang Mao, Frank W. R.Chaplen, Johan Grooten, Kris Gevaert, Joel Vandekerckhove,and Katia Vancompernolle(2001)Tumor necrosis factor-induced modulation of glyoxalase 1 activities through phosporylation by the PKA results in cell death and is accompanied by the formation of a specific methylglyoxal-derived AGE .Medical Sciences. vol.99:949-954

55.Gerlach, U. and Hiby, W.(1974)in Methods of Enzymatic Analysis(Bergmeyer, H.U.ed) 2nd ed .,Volume II, 569-573,Academic Press Inc.,NEW York, NY
56.Holcomb GN.Klemm La,Dulin WE(1974).The polyol pathway for glucose metabolism in tissues from normal , disbetic,and ketotic Chinese hamsters.Diabetologia .10 : 549-53
57. Greene DA,Lattimer SA,Sima AAF.Sorbitol(1987), phosphoinositides,and sodium-potas-sium-AT-Pase in the pathogenesis of diabetic complications.Nengl J Med .316 : 599-606

58. 郭素娥。(2002) 寡木糖對高膽固醇血症患者降血脂效果的研究。靜宜大學食品營養學系論文。

59. 陳瑋。(2005) 寡木糖對第二型糖尿病患者體內氧化及生理狀況之影響。靜宜大學食品營養學系論文。

60. 張雅涵。(2006) 寡木糖對第二型糖尿病患者血脂肪影響之研究。靜宜大學食品營養學系論文。

61. Takeuchi, M., Makita, Z.,Bucala, R.,Suzki, T.,Koike,&Kamda,Y (2000) Immunological evidence that non-carboxymethyllsine advanced glycation end-products are produced from short chain sugars and dicarbonyl compounds in vivo .Mol. Med. 6(2):114-125

62. Brownlee, M(2001) Biochemistry and molecular cell biology of diabetic complications. Nature .414(6865):813-820

63. Tahri, K.Grill, J. P. & Schneider, F.(1996) Bifidobacteria strain behavior toward cholesterol :coprecipitation with bile salts and assimilation.
Current Microbiology. 33(3):187-193

64. Roos, N. M. & Katan, M. B.(2000) Effects of probiotic bacteria on diarrhea,
lipid metabolism, and carcinogensis:a review of papers published between 1988 and 1998 . Am. J. Clin. Nutr. 71:405-411

65. Fiordalido, M. Kok, N., Desager, J. P., Goethals, F., Deboyser, D., Roberfroid, M. & Delzenne , N .(1995) Dietary oligofructose lowers triglycerides, phospholipids and cholesterol in serum and very low density lipoproteins of rat . Lipids .30:163-167

66. Bhatia, S., Shukla, R., Venkata, M. S., Kaur, G. J. & Madhava, P. K. (2003)
Antioxidant status ,lipid peroxidation and nitric oxide end products in patients of type 2 diabetes mellitus with nephropathy . Clin Biochem.36:557-562

67. Aydin, A., Ozata, M., Sahin, G. Isimer, A (2001) Oxidative stress and nitric oxide related parameters in type 2 diabetes mellitus:effects of glycemic control . Clin. Biochem. 34:65-70

68.Braaten, J. T.,Wood, P. J.,Scott, F. W.,Riedel, K. D.,Poste, L. M. and Collins, M.W.(1991)Oat gums lower glucose and insulin after an oral glucose load. Am. J. Clin. Nutr. 53:455-459

69.Torsdottir, I., Alpsten, M.,Anderson, H. and Einarsson, S(1989)Dietaty guar gum effects on postprandial glucose, insulin and hydroxyproline in humans .J. Nutr.119:1925-1931




QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top