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研究生:張嘉斐
研究生(外文):Chia-fei
論文名稱:Leptin、 Ghrelin 、Glutathione以及oxidation在於第二型糖尿病人與正常人兩者血液間之比較
論文名稱(外文):Comparison between type 2 diabetes and normal persons on leptin, ghrelin, glutathione and oxidation
指導教授:殷梅津殷梅津引用關係
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:營養學研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:37
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因此本實驗收集了40位第二型糖尿患者及15位正常人血液樣本,利用商業檢驗套組來分析,以比較第二型糖尿病人與正常人兩者血液間之Leptin、Ghrelin、 麩胱苷肽(Glutathione; GSH)濃度,以及oxidation情形。
本實驗結果為:在第二型糖尿病女性病人其Leptin濃度比男性病人高亦比正常女性Leptin濃度高,都有統計學上的顯著差異(P<0.05);在Ghrelin濃度則是男性糖尿病人Ghrelin濃度較正常人高,女性糖尿病人Ghrelin濃度比正常人較低,不論男女糖尿病人與正常人相比皆無統計學上的顯著差異(P<0.05);男性糖尿病人GSH濃度比正常人高,並且兩者相比有統計學上的顯著差異(P<0.05);另外糖尿病人不論男女其MDA值及HbA1c濃度皆比正常人高,並且有統計學上的顯著差異(P<0.05)。顯示出糖尿病人確實比正常人有較高的氧化及醣化傷害壓力存在著,因此在減緩糖尿病相關心血管併發症的發生是值得注意的。

Diabetes is one of the major chronic diseases in Taiwan. The impact of leptin and ghrelin upon diabetic progression has been paid attention. This study included 40 type II diabetic patients and 15 healthy controls. Blood levels of leptin, ghrelin, GSH and lipid oxidation were analyzed. Results showed that diabetic female had significantly higher leptin than diabetic male (P&lt;0.05), diabetic male had significantly higher ghrelin than diabetic female (P&lt;0.05). Diabetic patients has greater level of GSH than healthy control (P&lt;0.05). Both oxidation level, determined as MDA, and HbAlc level were significantly greater in diabetic patients (P&lt;0.05). These results indicated that the influence of leptin, ghrelin and HbAlc in diabetic patients needs to be paid more attention.

目錄………………………………………………………………………1
中文摘要…………………………………………………………………3
英文摘要…………………………………………………………………4
第一章、文獻回顧………………………………………………………5
一、糖尿病……………………………………………………..5
二、Leptin與糖尿病…………………………………………...9
三、Ghrelin與糖尿病………………………………………...10
四、麩胱苷肽與糖尿病………………………………………11
第二章、研究目……………………………………………………..13
第三章、材料與方法..................................14
一、實驗材料與樣本....................................14
(一)實驗樣本
(二)化學試藥
(三)分析檢驗套組
(四)儀器設備
二、實驗方法………………………………………………..…16
(一)實驗樣本
(二)實驗分析方法
三、統計分析………………………………………………….19
第四章、結果………………………………………………..21
一、第二型糖尿病病患相關檢驗……………………………21
二、正常人與糖尿病人血液中Leptin的濃度………………21
三、正常人與糖尿病人血液中Ghrelin的濃度..................21
四、正常人與糖尿病人血液中麩胱苷肽GSH的濃度...........22
五、正常人與糖尿病人血液中丙二醛MDA的濃度..............22
表一、實驗組第二型糖尿病病患相關特徵……………………23
圖一、正常人與糖尿病人血液中Leptin的濃度………………24
圖二、正常人與糖尿病人血液中Ghrelin的濃度……………25
圖三、正常人與糖尿病人血液中麩胱苷肽GSH的濃度……26
圖四、正常人與糖尿病人血液中丙二醛MDA的濃度………27
第五章、討論……………………………………………………28
一、Leptin、Ghrelin與糖尿病………………………………28
二、GSH、MDA與糖尿病…………………………………..30
第六章、結論………………………………………………………32
第七章、參考文獻……………………………………………………33


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