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研究生:劉雅婷
研究生(外文):Ya-Ting Liu
論文名稱:類雙黃酮對三種癌細胞株之作用
論文名稱(外文):Effect of biflavanoids on three cancer cells
指導教授:林榮芳林榮芳引用關係林玉美林玉美引用關係
指導教授(外文):Rong-Fong LinYuh-Meei Lin
學位類別:碩士
校院名稱:中臺科技大學
系所名稱:醫學生物科技研究所
學門:醫藥衛生學門
學類:醫學技術及檢驗學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:70
中文關鍵詞:hinokiflavonerobustaflavoneamentoflavoneTE671DBTRG細胞凋亡A549
外文關鍵詞:hinokiflavonerobustaflavoneapoptosisA549TE671DBTRGamentoflavone
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中草藥的研究是近年的熱門主題。早期研究指出,類黃酮(flavanoids)可抑制癌細胞生長或造成細胞凋亡(apoptosis),但類雙黃酮(biflavanoids)對癌細胞之影響則少有研究報導。本論文利用三種類雙黃酮 hinokiflavone、robustaflavone和amentoflavone分別處理人類細胞株橫紋肌肉瘤(rhabdomyosarcoma)細胞TE671、惡性腦瘤(brain glioblastoma)細胞DBTRG 及非小細胞肺癌(lung adenocarcinoma)細胞A549。結果顯示,hinokiflavone對TE671和DBTRG的IC50 (50% inhibiting concentration)分別約為5.8 μM和40.3 μM,而A549細胞之IC50則大於74.4 μM;robustaflavone和amentoflavone對TE671、DBTRG及A549的IC50 皆大於74.4 μM。由於hinokiflavone對三株細胞之抑制較為顯著,故後續實驗以hinokiflavone探討其抑制細胞生長之作用機制。RT-PCR的分析發現,hinokiflavone可抑制三株細胞brca2 mRNA之表現。DNA fragmentation 分析發現三種細胞株都無DNA 斷裂現象。Western 分析顯示,TE671、DBTRG和A549之p53和caspase 9表現量有明顯增加。此外,三種細胞活化型的caspases 3有表現,推測hinokiflavone可能會造成三株細胞的細胞凋亡。
Flavanoids have been reported to inhibit proliferation or result in apoptosis of cancer cells. However, effects of biflavanoids on cancer cells have not yet been described. In this study, three kinds of biflavanoids hinokiflavone, robustaflavone, and amentoflavone were used to treat human cancer cell lines TE671 (rhabdomyosarcoma), DBTRG (brain glioblastoma), and A549 (lung adenocarcinoma). The IC50 of hinokiflavone on TE671 and DBTRG were 5.8 μM and 40.3 μM, respectively, but that on A549 was more than 74.4 μM. On the other hand, the IC50 of robustaflavone and amentoflavone on TE671, DBTRG, and A549 were all more than 74.4 μM. The effect of hinokiflavone on growth inhibition of cancer cells was most significant. Therefore, the studies of the mechanism of action on inhibiting cancer cells by hinokiflavone was conducted. RT-PCR analysis revealed that the expression of brca2 mRNA was inhibited in the three cell lines. No DNA fragmentation was found in the three cell lines. The protein levels of p53 and caspase 9 increased in hinokiflavone-treated TE671, DBTRG and A549. The active form of caspase 3 was detected. Therefore, the inhibition of hinokiflavone aganist cancer cells possibly is through apoptosis.
中文摘要 ……………………………………………………………………Ⅰ
英文摘要 …………………………………………………………………… Ⅱ
目錄 ………………………………………………………………………… Ⅲ
序論 ………………………………………………………………………… 1
材料與方法 ………………………………………………………………… 13
結果 ………………………………………………………………………… 19
討論 ………………………………………………………………………… 21
結論 ………………………………………………………………………… 25
參考文獻 …………………………………………………………………… 26
圖表 ………………………………………………………………………… 38
附錄一……………………………………………………………………… 61
附錄二 ……………………………………………………………………… 64
黃旭輝。黃修偉。1992。橫紋肌肉瘤。國防醫學14:337-341。

陳冠助。1997。腦瘤的生化特性及其分類。國防醫學24:365-368。

劉敏英。1997。腦瘤之成因與統計結果。國防醫學24:363-364。

朱燕華。1998。類黃酮之介紹。食品工業月刊30:1-5。

陳育民。2002。肺癌新抗癌藥物之藥理機轉。臨床醫學50:223-230。

陳晉興。2003。認識肺癌 系列一:病因及分期。健康世界225:79-82。

陳發清。2005。木蠟色素及其藥效研究(虞兆中先生九秩壽慶文集)。

季匡華。2006。癌症治療新領域。聲洋防癌之聲114:2-6。

張季平。2006。談細胞壞死與細胞凋亡的關係。當代醫學33:86-92。

Atsuko, S., K. Matsunaga, Y. Mimaki, Y. Sashida, and Y. Ohizumi. 1999. Properties of amentoflavone, a potent caffeine-like Ca2+ releaser in skeletal muscle sarcoplasmic reticulum. Eur. J. Pharmacol. 372:97-102.

Banerjee, T., A. van der Vliet, and V. A. Ziboh. 2002. Down regulation of COX-2 and iNOS by amentoflavone and quercetin in A549 human adenocarcinoma cell line. Prostagl. Leukot. Essent. Fat. Acids 66:485-492.

Beneke, R., C. Geisen, B. Zevnik, T. Bauch, W. U. Muller, J. H. Kupper, and T. Moroy. 2000. DNA excision repair and DNA damage-induced apoptosis are linked to poly (ADP-ribosyl) ation but have different requirements for p53. Mol. Cell Biol. 20:6695-6703.

Bryan, C. B. and M. E. Peter. 2003. The TNF receptor 1: a split personality complex. Cell 144:181-190.

Byrd, J. C., C. Shinn, J. K. Waselenko, E. J. Fuchs, T. A. Lehman, P. L. Nguyen, I. W. Flinn, L. F. Diehl, E. Sausville, and M. R. Grever. 1998. Flavopiridol induces apoptosis in chronic lymphocytic leukemia cells via activation of caspase-3 without evidence of bcl-2 modulation or dependence on functional
p53. Blood 10:3804-3816.

Chen, F. C. 1948a. Acta Chim. Taiwanica 1:57.

Chen, F. C. 1948b. Acta Chim. Taiwanica 1:63.

Chen, F. C. 1950. J. Taiwan Pharm. Assoc. 2:20.

Chen, F. C., Y. M. Lin, and C. M. Liang. 1974. Biflavonyls from drupes of Rhus succedanea. Phytochemistry. 12:276-277.

Chen, F. C. and Y. M. Lin. 1976. Rhusflavanone, a new biflavanone from the seeds of wax-tree. J. Chem. Soc., Perkin Trans. I:98-101.

Chen, Y. C., S. C. Shen, W. R. Lee, F. L. Hsu, H. Y. Lin, C. H. Ko, and S. W. Tseng. 2002. Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production. Biochem. Pharmacol. 64:1713-1724.

Chen, J. J., C. Y. Duh, and J. F. Chen. 2005. New cytotoxic biflavonoids from Selaginella delicatula. Planta Med. 71:659-665.

Chung, L. Y., T. C. Cheung, S. K. Kong, K. P. Fung, Y. M. Choy, Z. Y. Chang, and T. T. Kwok. 2001. Induction of apoptosis by green tea catechins in human prostate cancer DU145 cells. Life Sci. 68:1207-1214.

Cohen, G. M., X. M. Sun, R. T. Snowden, D. Dinsdale, and D. N. Skilleter. 1992. Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation. Biochem. J. 286:331-334.

Cohen, L. H., E. Pieterman, R. E. W. van Leeuwen, J. Du, P. N. Aminou, A. Rob, P. M. Valentijn, M. Overhand, G. A. van der Marel, and J. H. van Boom. 1999. Inhibition of human smooth muscle cell proliferation in culture by farnesyl pyrophosphate analogues, inhibitors of in vitro protein: farnesyl transferase. Biochem. Pharmacol. 57:365-373.

Darja, K., A. Mittelman, R. Serpico, E. Sinigaglia, A. A. Sinha, C. Natale, R. Santacroce, M. G. D. Corcia, A. Lucchese, L. Dini, P. Pani, S. Santacroce, S. R. Bucci, and E. Farber. 2002. Cell death:apoptosis versus necrosis (review). Internat. J. Oncol. 21:165-170.

Daumas-Duport C., B. Scheithauer, J. O. Fallon, and P. Kelly. 1988. Grading of astrocytomas. A simple and reproducible method. Cancer 62:2152-2165.

Ding, H. F, G. McGill, S. Rowan, C. Schmaltz, A. Shimamura, and D. E. Fisher. 1998. Oncogene-dependent regulation of caspase activation by p53 protein in a cell-free system. J. Biol. Chem. 273:28378-28383.

Ding, H. F, G. McGill, S. Rowan, C. Schmaltz, A. Shimamura, and D. E. Fisher. 2000. Essential role for caspase-8 in transcription-independent apoptosis triggered by p53. J. Biol. Chem. 275:38905-38911.

Elliott, M. J. R., C. Kandaswami, and T. C. Theoharides. 2000. The effects of plant flavonoids on mammalian cells:implications for inflammation, heart disease, and cancer. Pharmacol. Rev. 52:673-751.

Ellis, H. M. and H. R. Horvitz. 1986. Genetic control of programmed cell death in the nematode C. elegans. Cell 44:817-29.

Elmore, L. W., C. W. Rehder, X. Di, P. A. McChesney, C. K. Jackson-Cook, D. A. Gewirtz, and S. E. Holt. 2002. Adriamycin-induced senescence in breast tumor cells involves functional p53 and telomere dysfunction. J. Biol. Chem. 277:35509-35515.

Furukawa. 1932. Studies on the constituents of Ginkgo leaves. Sci. Pap. Inst. Phys. Chem. Res. 19:27-38.

George, B. C., L. Fix, D. P. Jones, M. T. Moslen, P. Nicotera, F. A. Oberhammer, and R. Buttyan. 1994. Apoptosis:molecular control point in toxicity. Toxicol. Appl. Pharmacol. 128:169-181.

Gerald, M. C. 1997. Caspases: the executioners of apoptosis. Biochem. J. 326:1-16.

Gloria, L. S., H. Chai, M. P. Gupta, N. R. Farnsworth, G. A. Cordell, J. M. Pezzuto, C. W. W. Beecher, and A. D. Kinghorn. 1995. Cytotoxic biflavonoids from Selaginella willdenowii. Phytochemistry 40:129-134.

Gross, A., M. M. James, and J. K. Stanley. 1999. Bcl-2 family members and the mitrochondria in apoptosis. Genes Develop. 13:1899-1911.

Grynberg, N. F., M. G. Carvalho, J. R. Velandia, M. C. Oliveira, I. C. Moreira, R. Braz-Filho, and A. Echevarria. 2002. DNA topoisomerase inhibitors : biflavonoids from Ouratea species. Braz. J. Med. Biol. Res. 35:819-822.

He, Z., W. Y. Ma, T. Hashimoto, A. M. Bode, C. S. Yang, and Z. Dong. 2003. Induction of apoptosis by caffeine is mediated by the p53, Bax, and caspase 3 pathways. Cancer Res. 63:4396-4401.

Huang, K. C. 1999a. Anticancer herbs. In:Pharmacol. Chinese herbs, 2 nd ed. pp. 469-472 (New York).

Huang, K. C. 1999b. Anticancer herbs. In:Pharmacol. Chinese herbs, 2 nd ed. pp. 458-459 (New York).

Huang, K. C. 1999c. Anticancer herbs. In:Pharmacol. Chinese herbs, 2 nd ed. pp. 467-468 (New York).

Huang, Y. L., C. F. Tseng, L. K. Yang, and C. H. Tsai. 2005. Rhabdomyosarcoma of the adult nasopharynx : a case report. J. Inter. Med. Taiwan 16:146-150.

Hunakova, L., J. Sedlak, M. Klobusicka, J. Duraj, and B. Chorvath. 1994. Tyrosine kinase inhibitor-induced differentiation of K-562 cells: Alterations of
cell cycle and cell surface phenotype. Cancer Lett. 81:81–87.
Hussein, M. A., D. Chen, B. Cherney, K. Bhatia, V. Notario, C. Girit, G. Steint, E. Slattery, R. G. Roeder, and M. E. Smulson. 1987. Cloning and expression of cDNA for human poly (ADP-ribose) polymerase. Proc. Natl. Acad. Sci. 84:1224-1228.

Inna, L., A. Golks, and P. H. Krammer. 2005a. Death receptor signaling. J. Cell Sci. 118:265-267.

Inna, L., A. Golks, and P. H. Krammer. 2005b. Caspases : pharmacological manipulation of cell death. J. Clin. Invest. 115:2665-2672.

Kang, S. S., J. Y. Lee, Y. K. Choi, S. S. Song, J. S. Kim, S. J. Jeon, Y. N. Han, K. H. Son, and B. H. Han. 2005. Neuroprotective effects of naturally occurring biflavonoids. Bioorg. Med. Chem. Lett. 15:3588-3591.

Kerr, J. F., A. H. Wyllie, and A. R. Currie. 1972. Apoptosis:a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer 26:239-257.

Kim, W. J., D. I. Beardsley, A. W. Adamson, and K. D. Brown. 2005. Themonofunctional alkylating agent N-methyl-N′-nitro-N-nitrosoguanidine triggers apoptosis through p53-dependent and -independent pathways. Toxicol. Appl. Pharmacol. 202:84-98.

Kiyotsugu, Y. and M. Yoshio. 2004. Role of BRCA1 and BRCA2 as regulators of DNA repair, transcription, and cell cycle in response to DNA damage. Cancer Sci. 95:866-871.

Klaus, S. O., H. Walczak, W. Droge, and P. H. Krammer. 1994. Cell nucleus and DNA fragmentation are not required for apoptosis. J. Cell Biol. 127:15-20.

Konoshima, T., M. Takasaki, M. Kozuka, Y. M. Lin, F. C. Chen, H. Tokuda, and T. Matsumoto. 1988. Studies on inhibitors of skin tumor promotion (IV): inhibitory effects of flavonoids on Epstein-Barr Virus activation (1). Shoyakugaku Zasshi 42:343-346.

Lazebnik, Y. A., S. H. Kaufmann, S. Desnoyers, G. G. Poirier, and W. C. Earnshaw. 1994. Cleavage of poly(ADP-ribose)polymerase by a proteinase with properties like ICE. Nature 371:346-347.

Lee, S. J., J. H. Choi, K. H. Son, H. W. Chang, S. S. Kang, and H. P. Kim. 1995. Suppression of mouse lymphocyte proliferation in vitro by naturally-occurring biflavonoids. Life Sci. 57:551-558.

Lee, H. S., W. K. Oh, B. Y. Kim, S. C. Ahn, D. O. Kang, D. I. Shin, J. Kim, T. I. Mheen, and J. S. Ahn. 1996. Inhibition of phospholipase C gamma 1 activity by Selaginella tamariscina. Planta Med. 62:293-296.

Lee, H., A. H. Trainer, L. S. Friedman, F. C. Thistlethwaite, M. J. Evans, B. A. Ponder, and A. R. Venkitaraman. 1999. Mitotic checkpoint inactivation fosters
transformation in cells lacking the breast cancer susceptibility gene, Brca2. Mol. Cell 4:1-10.

Lin, Y. M., and Chen F. C. 1974. Robustaflavone from the Seed-Kernels of
Rhus Succedanea. Phytochemistry. 13:1617-1619.

Lin, Y. M., F. C. Chen, and K. H. Lee. 1989. Hinokiflavone, a cytotoxic principle from Rhus succedanea and the cytotoxicity of the related biflavonoids. Planta Med. 55:166-168.

Lin, Y. M., H. Anderson, M. T. Flavin, and Y. H. S. Pai. 1997a. In vitro anti-HIV activity of biflavonoids isolated from Rhus succedanea and Garcinia multiflora. J. Nat. Prod. 60:884-888.

Lin, Y. M., D.E. Zembower, M. T. Flavin, R. M. Schure, H. M. Anderson, B. E. Korba, and F. C. Chen. 1997b. Robustaflavone, a naturally occurring biflavanoid, is a potent non-nucleoside inhibitor of Hepatitis B Virus replication
in vitro. Biorg. Med. Chem. Lett. 7:2325-2328.

Lin, Y. M., M. T. Flavin, R. Schure, F. C. Chen, R. Sidwell, D. L. Barnard, J. H. Huffman, and E. R. Kern. 1999. Antiviral activities of biflavonoids. Planta Med. 65:120-125.

Lin, L. C., Y. C. Kuo, and C. J. Chou. 2000. Cytotoxic biflavonoids from Selaginella delicatula. J. Nat. Prod. 63:627-630.

Lin, Y. M., M. T. Flavin, C. S. Cassidy, A. Mar, and F. C. Chen. 2001. Biflavonoids as novel antituberculosis agents. Biorg. Med. Chem. Lett. 11:2101-2104.

Lopez-Lazaro, M. 2002. Flavonoids as anticancer agents : structure-activity relationship study. Curr. Med. Chem.-Anti-Cancer Agents 2:691-714.

Ma, S. C., P. P. H. But, V. E. C. Ooi, Y. H. He, S. H. S. Lee, S. F. Lee, and R. C. Lin. 2001. Antiviral amentoflavone from Selaginella sinensis. Biol. Pharm. Bull. 24:311-312.

Manthey, J. A. and G. Najla. 2002. Antiproliferative activities of citrus flavonoids against six human cancer cell lines. J. Agric. Food Chem. 50:5837-5843.

Michael, O. H. 2000. The biochemistry of apoptosis. Nature 407:770-776.

Nicholson, D. W., A. Ali, N. A. Thornberry, J. P. Vaillancourt, C. K. Ding, M. Gallant, Y. Gareau, P. R. Griffin, M. Labelle, Y. A. Lazebnik, N. A. Munday, S. A. Raju, M. E. Smulson, T. T. Yamin, V. L. Yu, and D. K. Miller. 1995. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376:37-43.

Pagliacci, M. C., F. Spinozzi, G. Migliorati, G. Fumi, M. Smacchia, F. Grignani, C. Riccardi, and I. Nicoletti. 1993. Genistein inhibits tumour cell growth in vitro but enhances mitochondrial reduction of tetrazolium salts: A further pitfall in the use of the MTT assay for evaluating cell growth and survival. Eur. J. Cancer
29A:1573-1577.

Pan, X., N. Tan, G. Zeng, Y. Zhang, and R. Jia. 2005.Amentoflavone and its derivatives as novel natural inhibitors of human Cathepsin B. Bioorg. Med. Chem. 13:5819-5825.

Park, J. B. 1999. Flavonoids are potential inhibitors of glucose uptake in U937 cells. Biochem. Biophys. Res. Commun. 260:568-574.

Porta1, C., H. S. Reda, N. Mohamed, P. Mathieu, M. G. Tovey1, E. Lucile, A. Sandra, and K. C. A. Mounira . 2005. Interferons α and γ induce p53-dependent and p53-independent apoptosis, respectively. Oncogene 24:605-615.

Pritchard, D. M., A. J. Watson, C. S. Potten, A. L. Jackman, and J. A. Hickman. 1997. Inhibition by uridine but not thymidine of p53 dependent intestinal apoptosis initiated by 5-fluorouracil:evidence for the involvement of RNA perturbation. Proc. Natl. Acad. Sci. USA 94:1795-1799.

Reed, I. C., S. Tanaka, and M. Cuddy. 1992. Cell cycle analysis of p26-BCL-2 protein levels in proliferating lymphoma and leukemia cell lines. Cancer Res.
52:2802–2805.

Robert, J. N., E. van Nood, D. E. C. van Hoorn, P. G. Boelens, K. van Norren, and P. A. M. van Leeuwen. 2001. Flavonoids : a review of probable mechanisms of action and potential applications. Am. J. Clin. Nutr. 74:418-25.

Sagar, S. M., D. Yance, and R. K. Wong. 2006. Natural health products that inhibit angiogenesis: a potential source for investigational new agents to treat cancer-Part 2. Curr. Oncol. 13:99-107.

Schuler, M., B. W. Ella, J. C. Goldstein, P. Fitzgerald, and D. R. Green. 2000. p53 induces apoptosis by caspase activation through mitochondrial cytochrome
c release. J. Biol. Chem. 275:7337-7342.

Sharad, K. 1995. ICE-like protease in apoptosis. Trends Biochem. Sci. 20:198-202.

Shigekazu, N. 2000. Apoptotic DNA fragmentation. Exp. Cell Res. 256:12-18.

Shimizu T. and Y. Pommier. 1997. Camptothecin-induced apoptosis in p53-null human leukemia HL60 cells and their isolated nuclei: effects of the protease inhibitors Z-VAD-fmk and dichloroisocoumarin suggest an involvement of both
caspases and serine proteases. Leukemia 11:1238-1244.

Solange, D. and J. C. Martinou. 2000. Mitochondria as the central control point of apoptosis. Trends Cell Biol. 10:369-377.

Sun, C. M., W. J. Syu, Y. T. Huang, C. C. Chen, and J. C. Ou. 1997. Selective cytotoxicity of ginkgetin from Selaginella moellendorffii. J. Nat. Prod. 60:382-384.

Traganos, F., B. Ardelt, M. Halko, S. Bruno, and Z. Darzynkiewicz. 1992. Effects of genistein on the growth and cell cycle progression of normal human lymphocytes and human leukemic MOLT-4 and HL-60 cells. Cancer Res.
52:6200-6208.

Von, M. L. L., J. L. Weemhoff, E. Bedir, I. A. Khan, J. S. Harmatz, P. Goldman, and D. J. Greenblatt. 2004. Inhibition of human cytochromes P450 by components of Ginkgo biloba. J. Pharm. Pharmacol. 56:1039-1044.

Wall, M., M. C. Wani, F. Fullas, J. B. Oswald, D. M. Brown, T. Santisuk, V. Reutrakul, A. T. McPhail, N. R. Farnsworth, and J. M. Pezzuto. 1994. Plant antitumor agents. 31. The calycopterones, a new class of biflavonoids with novel cytotoxicity in a diverse panel of human tumor cell lines. J. Med. Chem. 37:1465-1470.

Welcsh, P. L., K. N. Owens, and M.C. King. 2000. Insights into the functions of BRCA1 and BRCA2. Trends Genet. 16:69-74.

Woo, E. R., J. Y. Lee, I. J. Cho, S. G. Kim, and K. W. Kang. 2005. Amentoflavone inhibits the induction of nitric oxide synthase by inhibiting NF-κB activation in macrophages. Pharmacol. Res. 51:539-546.
Yoshida, M., M. Yamamoto, and T. Nikaido. 1992. Quercetin arrests human leukemic T-cells in late G1-phase of the cell cycle. Cancer Res. 52:6676–6681.

Yuan, J., S. Shaham, S. Ledoux, H. M. Ellis, and H. R. Horvitz. 1993. The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme. Cell 75:641-652.

Zembower, D. E., Y. M. Lin, M. T. Flavin, F. C. Chen, and B. E. Korba. 1998. Robustaflavone, a potential non-nucleoside anti-hepatitis B agent. Antiviral Res. 39:81-88.

Zeng, G. Z., X. L. Pan, N. H. Tan, J. Xiong, and Y. M. Zhang. 2006. Natural biflavones as novel inhibitors of cathepsin B and K. Bioorg. Med. Chem. 41:
1247-1252.

Zhiwei, H., W. Y. Ma, T. Hashimoto, A. M. Bode, C. S. Yang, and Z. Dong. 2003. Induction of apoptosis by caffeine is mediated by the p53, Bax, and caspase 3
pathways. Cancer Res. 63:4396-4401.
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