中草藥的研究是近年的熱門主題。早期研究指出，類黃酮(flavanoids)可抑制癌細胞生長或造成細胞凋亡(apoptosis)，但類雙黃酮(biflavanoids)對癌細胞之影響則少有研究報導。本論文利用三種類雙黃酮 hinokiflavone、robustaflavone和amentoflavone分別處理人類細胞株橫紋肌肉瘤(rhabdomyosarcoma)細胞TE671、惡性腦瘤(brain glioblastoma)細胞DBTRG 及非小細胞肺癌(lung adenocarcinoma)細胞A549。結果顯示，hinokiflavone對TE671和DBTRG的IC50 (50% inhibiting concentration)分別約為5.8 μM和40.3 μM，而A549細胞之IC50則大於74.4 μM；robustaflavone和amentoflavone對TE671、DBTRG及A549的IC50 皆大於74.4 μM。由於hinokiflavone對三株細胞之抑制較為顯著，故後續實驗以hinokiflavone探討其抑制細胞生長之作用機制。RT-PCR的分析發現，hinokiflavone可抑制三株細胞brca2 mRNA之表現。DNA fragmentation 分析發現三種細胞株都無DNA 斷裂現象。Western 分析顯示，TE671、DBTRG和A549之p53和caspase 9表現量有明顯增加。此外，三種細胞活化型的caspases 3有表現，推測hinokiflavone可能會造成三株細胞的細胞凋亡。

Flavanoids have been reported to inhibit proliferation or result in apoptosis of cancer cells. However, effects of biflavanoids on cancer cells have not yet been described. In this study, three kinds of biflavanoids hinokiflavone, robustaflavone, and amentoflavone were used to treat human cancer cell lines TE671 (rhabdomyosarcoma), DBTRG (brain glioblastoma), and A549 (lung adenocarcinoma). The IC50 of hinokiflavone on TE671 and DBTRG were 5.8 μM and 40.3 μM, respectively, but that on A549 was more than 74.4 μM. On the other hand, the IC50 of robustaflavone and amentoflavone on TE671, DBTRG, and A549 were all more than 74.4 μM. The effect of hinokiflavone on growth inhibition of cancer cells was most significant. Therefore, the studies of the mechanism of action on inhibiting cancer cells by hinokiflavone was conducted. RT-PCR analysis revealed that the expression of brca2 mRNA was inhibited in the three cell lines. No DNA fragmentation was found in the three cell lines. The protein levels of p53 and caspase 9 increased in hinokiflavone-treated TE671, DBTRG and A549. The active form of caspase 3 was detected. Therefore, the inhibition of hinokiflavone aganist cancer cells possibly is through apoptosis.

中文摘要 ……………………………………………………………………Ⅰ
英文摘要 …………………………………………………………………… Ⅱ
目錄 ………………………………………………………………………… Ⅲ
序論 ………………………………………………………………………… 1
材料與方法 ………………………………………………………………… 13
結果 ………………………………………………………………………… 19
討論 ………………………………………………………………………… 21
結論 ………………………………………………………………………… 25
參考文獻 …………………………………………………………………… 26
圖表 ………………………………………………………………………… 38
附錄一……………………………………………………………………… 61
附錄二 ……………………………………………………………………… 64

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