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研究生:蘇苑菱
研究生(外文):Su Yuan-Ling
論文名稱:八種藥用植物之抗氧化性研究
論文名稱(外文):Study on Antioxidative Properties of Eight Medicinal Herbs
指導教授:涂瑞澤涂瑞澤引用關係吳淑姿吳淑姿引用關係
指導教授(外文):J.R.TooS.T. Wu
學位類別:碩士
校院名稱:大葉大學
系所名稱:生物產業科技學系
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:119
中文關鍵詞:夜交藤檸檬香茅康復力刺葱羅勒楓香野薑咸豐草精油抗氧化
外文關鍵詞:Polygonum multiflorum ThunbCymbopogon citratesSymphytum officinale L.Zanthoxylum ailanthoids Sieb & zuccOcimum basilicumLiquidambar formosanaHedychium coronarium KoenigBidens pilosaessential oilantioxidant
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近年來許多研究致力於尋找具高抗氧化性且安全無毒害之天然物質,以減低生物體內之氧化性傷害。本研究選定八種不同植物:夜交藤(polygonum multiflorum Thunb)、檸檬香茅(Cymbopogon citrates)、康復力(Symphytum officinale L.)、刺葱(Zanthoxylum ailanthoids Sieb & zucc)、羅勒(Ocimum basilicum)、楓香(Liquidambar formosana)、野薑(Hedychium coronarium Koenig)及大花咸豐草(Bidens pilosa)作為研究材料,這些植物自古即常應用於藥用或食用,為具有多用途之中藥材。
本研究之目的為探討植物經水蒸氣蒸餾所得之精油或以50%乙醇為溶劑,進行熱迴流萃取之夜交藤、檸檬香茅、羅勒及康復力等四種地上全株植物萃取物與楓香、咸豐草、刺蔥及野薑等四種植物,不同部位分別萃取所得之萃取物的抗氧化能力。抗氧化成分與抗氧化活性試驗包括:總多酚類與類黃酮含量、對DPPH自由基的清除能力、亞鐵離子螯合能力、還原力、清除超氧陰離子能力、清除ABTS陽離子自由基能力及抑制微脂粒氧化作用能力等,並與BHA、EDTA、α-生育醇及沒食子酸之抗氧化性做比較。
實驗結果顯示,經水蒸氣蒸餾後可得精油,但其萃取率甚低,其中以楓香葉(0.06%)與野薑根(0.16%)較高。楓香葉與野薑根精油的總多酚含量分別為64.00與14.04 mg/g,其類黃酮含量分別為0.08與0.03 mg/g,在清除DPPH自由基試驗中,其清除率分別為15.06%與 5.59%;亞鐵離子螯合力試驗,其螯合力分別為46.88%與 16.38%。另外,以50%乙醇為溶劑進行熱迴流萃取,所得萃取物中以楓香葉之萃取率最高。在萃取物中,以夜交藤萃取物之總多酚含量(438.06 mg/g)為最高,其次為楓香莖,野薑根含量最低。類黃酮含量以野薑葉含量(16.42 mg/g)為最高。楓香葉對DPPH自由基之清除率(95.32%)為最佳;康復力之螯合亞鐵離子能力(99.46%)最佳。夜交藤、羅勒及咸豐草葉具有與α-生育醇相近之還原力。夜交藤、康復力、羅勒及檸檬香茅等四種植物於濃度小於0.1 mg/mL時,均比沒食子酸的超氧陰離子清除能力為佳,又以檸檬香茅和刺葱莖之超氧陰離子清除能力為最佳。夜交藤與羅勒於0.1 mg/mL時,與抗氧化劑BHA及α-生育醇之總抗氧化能力相近;夜交藤、康復力及檸檬香茅於10 mg/mL時,與α-生育醇之抑制微脂粒氧化作用能力相近,其抑制率為50%-60%。
綜合研究結果顯示,八種植物之萃取物皆具有抗氧化性,又以夜交藤與楓香莖之總多酚類化合物含量較多,且具有高的DPPH自由基清除能力、還原力、總抗氧化能力及抑制微脂粒氧化作用能力,可進行更深入的研究,將有助於機能性食品之開發,與後續更深入研究之基礎。
Recently, there is increasing interest in finding natural antioxidants from plants, which protect human body against free radical insult and ameliorate the progress of many chronic diseases. Essential oils and extracts from Eight plants with high antioxidativity were selected, including Polygonum multiflorum Thunb, Cymbopogon citrates, Symphytum officinale L., Zanthoxylum ailanthoids Sieb & zucc, Ocimum basilicum, Liquidambar formosana, Hedychium coronarium Koenig and Bidens pilosa. In this study, the antioxidative activities assays, including α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging effect, Fe2+ chelating power, reducing power, superoxide radical anion scavenging effect, trolox equivalent antioxidant capacity and the inhibition of Fe/ascorbate-induced lipid peroxidation in a liposome model system, were measured and compared with those of butylated hydroxyanisole (BHA), ethylene diamine tetracetic acid (EDTA), α–tocopherol and gallic acid.
The results showed that the yield of plant essential oil was lower than the solvent extract. All essential oils in eight plants had low flavonoids. The essential oils in leaves of Liquidambar formosana and the root of Hedychium coronarium Koenig had low DPPH radical scavenging effect and Fe2+ chelating power. The ethanol extracts of Polygonum multiflorum Thunb and leaves of Hedychium coronarium Koenig had the highest content of polyphenolic compounds and flavonoids, respectively. Polygonum multiflorum Thunb, Ocimum basilicum and the leaves of Bidens pilosa had the same reductivity as α-tocopherol. All these eight plants (concentration less than 0.1 mg/mL) had better scavenging ability of superoxide anion radical than that of the gallic acid. The Cymbopogon citrates and Zanthoxylum ailanthoids Sieb & zucc had the best scavenging ability of superoxide anion radical. Polygonum multiflorum Thunb and Ocimum basilicum had the same total antioxidant capacity as BHA and α-tocopherol. Polygonum multiflorum Thunb, Symphytum officinale L. and Cymbopogon citrates (concentration at 10 mg/mL) on the lipid peroxidation in a liposome model system hed a same ability as α-tocopherol.
目錄

封面內頁
簽名頁
授權書...........................................iii
中文摘要.........................................iv
英文摘要.........................................vi
誌謝............................................ viii
目錄............................................ix
圖目錄...........................................xiii
表目錄.......................................... xvi

1. 緒論......................................... 1
2. 文獻回顧......................................3
2.1精油簡介...................................3
2.1.1精油定義..............................3
2.1.2精油種類..............................3
2.1.3用途..................................6
2.2植物簡介...................................6
2.2.1楓香..................................6
2.2.2咸豐草................................8
2.2.3野薑.................................10
2.2.4刺蔥.................................12
2.2.5夜交藤...............................15
2.2.6康復力...............................15
2.2.7羅勒.................................18
2.2.8檸檬香茅..............................20
2.3萃取方式....................................20
2.3.1水蒸餾法...............................22
2.3.2水蒸氣蒸餾法...........................22
2.3.3水蒸氣蒸餾溶劑抽取法....................22
2.3.4有機溶劑抽取法.........................23
2.3.5冷吸法................................23
2.3.6壓榨法................................23
2.3.7超臨界流體萃取法.......................24
2.3.8熱迴流萃取............................24
2.4醫學之應用.................................24
2.5抗氧化劑...................................25
2.5.1抗氧化作用原理.........................25
2.5.2天然抗氧化物...........................30
2.5.3人工合成抗氧化劑.......................35
2.6自由基簡介.................................35
2.6.1 自由基與活性氧........................35
2.6.2 定義及反應形式........................38
2.6.3 種類.................................41
2.6.4 自由基對生物體分子之氧化傷害............44
2.7 抗氧化作用原理.............................46
3. 材料與方法.................................... 50
3.1植物材料...................................50
3.2試藥......................................50
3.3實驗設備...................................51
3.4實驗方法...................................51
3.4.1水蒸氣蒸餾............................52
3.4.2熱回流萃取............................52
3.4.3總多酚類化合物含量之測定................52
3.4.4類黃酮含量之測定.......................55
3.4.5清除α,α-diphenyl-β-picrylhydrazyl (DPPH) 自由基之測定..................................55
3.4.6 螯合亞鐵離子之測定....................55
3.4.7 還原力試驗...........................56
3.4.8 清除超氧陰離子之能力試驗...............56
3.4.9 清除ABTS陽離子自由基能力試驗...........56
3.4.10抑制微脂粒(Liposome)氧化作用之測定.....57
3.4.11抗氧化效力評估模式....................57
4. 結果與討論....................................59
4.1 植物精油與其萃取物之萃取率..................59
4.2 總多酚化合物含量...........................59
4.3 類黃酮含量................................ 61
4.4 清除α,α-diphenyl-β-picrylhydrazyl (DPPH) 自由基之效力...................................61
4.5 螯合亞鐵離子能力...........................71
4.6 還原力................................... 78
4.7 清除超氧陰離子之能力.......................85
4.8 清除ABTS陽離子自由基能力...................92
4.9 抑制微脂粒(liposome)氧化作用之效力..........99
5. 結論..........................................109
5.1 結論......................................109
5.2 未來展望...................................110
參考文獻..........................................111
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