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研究生:黃彥能
研究生(外文):Huang Yen Neng
論文名稱:白藜蘆醇對綠膿桿菌之抑制效果研究
論文名稱(外文):Study on Inhibitory Effect of Resveratrol on Growth of Pseudomonas aeruginosa
指導教授:張耀南張耀南引用關係
指導教授(外文):Chang Yaw Nan
學位類別:碩士
校院名稱:大葉大學
系所名稱:生物產業科技學系
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:59
中文關鍵詞:白藜蘆醇綠膿桿菌抑菌活性濃度
外文關鍵詞:resveratrolPseudomonas aeruginosaantimicrobial activityconcentration
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本研究將市售50%與98%純度之白藜蘆醇 ( resveratrol, 3,5,4’-trihydroxystilbene ) 分別溶於含有不同濃度DMSO ( Dimethyl sulfoxide ) 之 10% 乙醇溶液中,再進行其對綠膿桿菌( Pseudomonas aeruginosa ) 之抑菌效果探討。研究結果顯示,50% 與 98% 純度白藜蘆醇對綠膿桿菌具有相似之抑菌效果,一般而言,98% 純度白藜蘆醇的抑菌效果大於 50% 純度者,但實際上其差異性不顯著,在第 4 小時培養時間時,其抑菌效果達到最佳狀態,但與白藜蘆醇的添加濃度與純度似乎無任何關係;白藜蘆醇的抑菌效果隨即降低,在第 8~12 小時培養時間時,其抑菌效果為最差,而後其抑菌效果逐漸增加而至穩定狀態。另外,DMSO 添加濃度亦影響白藜蘆醇的抑菌效果,其抑菌效果在 0.8% DMSO 添加濃度下達到最佳狀態,但隨其添加濃度增加而有少許下降趨勢。最後,若以 1.0 mg/ml 白藜蘆醇添加於含有 0.8% DMSO 之 10% 乙醇溶液中,白藜蘆醇對綠膿桿菌的抑菌效果可能達到最佳狀態。
The antimicrobial effect of commercial 50% and 98% purity resveratrol ( 3,4,5’-trihydroxystilbene ) in 10% ethanol solution with different concentration ( 0.1~3.2% ) DMSO ( Dimethyl sulfoxide ) on the growth of Pseudomonas aeruginosa was investigated in this study. The antimicrobial activities of both 50% and 98% purity resveratrol against P. aeruginosa were similar. In general, the antimicrobial activity of 98% purity resveratrol was better than that of 50% purity one, but there was no significant difference between 50% and 98% purity ones. The optimal antimicrobial activity of resveratrol against the growth of P. aeruginosa was at the 4th hr of cultivation at 35oC and 150 rpm, while the inhibition of P. aeruginosa growth seemed not clearly vary with according to the addition concentration and purity of resveratrol. After 4 hr cultivation, the antimicrobial activity of resveratrol decreased as increasing the culture time and then became much low during 8~12 hr cultivation. After that, the activity gradually increased with culture time and then became unchangeable. In addition, the antimicrobial activity of resveratrol increased as increasing the addition concentration of DMSO when its concentration was less than 0.8%, while the activity less decreased when DMSO concentration was higher than 0.8%. For the best antimicrobial activity of resveratrol against the growth of P. aeruginosa, its optimal addition concentration may be 1.0 mg/ml in 10% ethanol solution with 0.8% DMSO in this study.
目錄

封面內頁
簽名頁
授權書 iii
中文摘要 iv
英文摘要 v
誌謝 vi
目錄 vii
圖目錄 ix
表目錄 xi

1. 前言 1
2. 文獻回顧 3
2.1 綠膿桿菌 3
2.2 綠膿桿菌感染方式與症狀 4
2.3 綠膿桿菌的致病機制 6
2.4 白藜蘆醇之特性 9
2.4.1 白藜蘆醇之抑菌性 18
2.4.2 白藜蘆醇之抗氧化性 18
2.4.3 白藜蘆醇之抗發炎力 19
2.4.4 白藜蘆醇之抗癌力 19
3. 材料與方法 20
3.1 實驗材料及方法 20
3.1.1 實驗材料 20
3.1.2 儀器設備 21
3.2 菌種培養方法 21
3.3 實驗方法 23
3.4 抑菌率之計算 24
4. 結果與討論 25
4.1 乙醇之抑菌性試驗 25
4.2 DMSO 界面活性劑之抑菌試驗 25
4.3 50% 白藜蘆醇之抑菌試驗 25
4.4 98% 白藜蘆醇之抑菌試驗 34
5. 結論 43
參考文獻 44

圖目錄

圖 2.1 藥用植物圖鑑-虎杖 11
圖 2.2 反式白藜蘆醇及其衍生物 17
圖 3.1 綠膿桿菌之生長曲線 22
圖 4.1 不同濃度乙醇(10%、50%、70%)對綠膿桿菌的生長曲線 26
圖 4.2 不同濃度 DMSO 對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 27
圖 4.3 50% 白藜蘆醇溶於含有 0.1% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 29
圖 4.4 50% 白藜蘆醇溶於含有 0.2% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 30
圖 4.5 50% 白藜蘆醇溶於含有 0.4% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 31
圖 4.6 50% 白藜蘆醇溶於含有 0.8% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 32
圖 4.7 50% 白藜蘆醇溶於含有 1.6% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 33
圖 4.8 50% 白藜蘆醇溶於含有 3.2% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 35
圖 4.9 98% 白藜蘆醇溶於含有 0.1% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 36
圖 4.10 98% 白藜蘆醇溶於含有 0.2% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 37
圖 4.11 98% 白藜蘆醇溶於含有 0.4% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 38
圖 4.12 98% 白藜蘆醇溶於含有 0.8% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 40
圖 4.13 98% 白藜蘆醇溶於含有 1.6% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 41
圖 4.14 98% 白藜蘆醇溶於含有 3.2% DMSO 之 10% 乙醇溶液中對綠膿桿菌之影響(a)生長曲線(b)抑菌活性 42

表目錄

表 2.1 綠膿桿菌致病相關因子與機制 7
表 2.2 反式白藜蘆醇之主要生物活性性質 10
表 2.3 不同農業資源中反式白藜蘆醇含量 12
表 2.4 不同紅酒商品反式白藜蘆醇含量 13
表 2.5 不同白酒商品反式白藜蘆醇含量 15
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