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研究生:梁玉菁
研究生(外文):Linng Yu-Jing
論文名稱:月桃種子之抗氧化及降血脂作用
論文名稱(外文):Studies on Invitro Antioxidative and Invivo Hypolipidemic Activities of the Alpinia zerumbet Seed
指導教授:游銅錫林麗雲林麗雲引用關係
指導教授(外文):Yu Tung-HisLin Li-Yun
學位類別:碩士
校院名稱:大葉大學
系所名稱:生物產業科技學系
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:109
中文關鍵詞:月桃種子精油萜烯類化合物倉鼠抗氧化降血脂
外文關鍵詞:Alpinia zerumbetessential oilterpeneshamsterantioxidantserum lipid lowering effect
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本研究以月桃種子為材料,經清洗、風乾、攪碎後以水蒸氣蒸餾萃取方式萃取精油,再以氣相層析儀及氣相層析質譜儀鑑定其揮發性成分,並探討月桃種子之水及乙醇萃取物之抗氧化性。最後,以動物試驗探討月桃種子之降血脂功效。研究結果如下:月桃種子的精油收率為0.51 %;精油中以萜烯類化合物為主要的成份,而其萜烯類化合物中又以單萜烯類化合物(monoterpenes)為其主要成份,其含量約佔總揮發性成分的77.58%。月桃種子精油經矽膠管柱區分,在正戊烷區分溶洗部分,主要的組成以單萜烯碳氫化合物為主,其含量約佔總揮發性成分的75.66%。乙醚溶洗部分中則以單萜烯含氧化物為主,倍半萜烯含氧化物為次主要組成,分別佔總揮發性成分的27.10%及24.53 %。另外,月桃種子精油經矽膠管柱區分後可發現,在正戊烷溶洗部分比未區分精油新鑑定到2種化合物,而乙醚溶洗部分則為新鑑定到7種化合物。
  在抗氧化活性分析中,DPPH自由基捕捉之能力以乙醇萃取物之能力較佳,濃度10 mg/mL時達到91.68 %;螯合亞鐵離子之能力以水萃取物之能力較佳,濃度100 mg/mL時達到52.46 %;在抑制油脂之自氧化能力上則以乙醇萃取物較水萃取物較佳,於濃度20 mg/mL時達到68.64%。在芸香苷(rutin)及槲皮酮(quercetin)含量分析結果,以乙醇萃取物之含量最高,每100公克含量分別達6.06mg及46.29 mg。另外在總酚部份結果,以乙醇萃取物之含量最高,每毫升達2033 mg。
  月桃種子之降血脂功效,以雄性倉鼠為實驗對象,探討添加0.01%、0.05%及0.1%月桃種子精油及添加1%、3%及5 %月桃種子粉末飼料餵食倉鼠8週,犧牲後分析其血液及肝臟中脂質型態,結果顯示餵食月桃種子精油組與粉末組皆可降低血清及肝臟中膽固(p<0.05),其中精油組以餵食0.05%濃度之精油效果最佳,粉末組則以餵食5%濃度之粉末效果最佳。兩組間相比則以餵食5%粉末降血脂的效果最好。可使血液及肝臟中三酸甘油脂、膽固醇、低密度脂蛋白膽固醇含量恢復至空白組之水平。
In this study, essential oil from Alpinia zerumbet seed were obtained using steam distillation and identified using GC and GC-MS.The antioxidant activities of the water and enthanol extracts of A. zerumbet seed were also studied. Finally, serum lipid lowering effect of A. zerumbet seed was conduct using animal test.
The results are as follows: the yields of essential oil of A. zerumbet seed is around were 0.51% . The major volatile compounds in the essential oil of A. zerumbet are found to be terpenes, especially the monoterpenes (around 77.58%). After fractionation using silica gel column, the major volatile compounds in pentane fraction of A. zerumbet seed essential oil are found to monoterpenes(around 75.66 %),in whereas the major volatile compounds found in the ether fraction of A. zerumbet seed essential oil are monoterpenes and oxygenated sequiterpenes(27.10 % and 24.53% respectively).
The ethanol extract of A. zerumbet seed was found to have stronger DPPH free radical scavenging capability (91.68% in 10 mg/mL dosage) than that of water extract. The water extract of A. zerumbet seed was found to have stronger ferric ion chelating effect capability (52.46% 100 mg/mL dosage ) than that of ethanol extract.The ethanol extract of A. zerumbet seed was found to have better linoleic acid oxidation inhibition capability(68.64% 20 mg/mL dosage ) than that of water extract.The ethanol extract of A. zerumbet seed has higher rutin and quercetion contents(6.06 mg and 46.29 mg per 100 gram, respectively)than that of water extract. The ethanol extracts of A. zerumbet seed has higher content of total polyphenol (2033 mg per mL) than that of water extract.
In the essential oil and powder feeding study of A. zerumbet using the male hamsters, the male hamsters were fed with a controlled feed or a controlled feed plus 0.01 ,0.05, or 0.1% A. zerumbet seed essential oil or plus 1,3 and 5 % A. zerumbetseed powder. Both of the essential and the seed power of A. zerumbet were found to -viilower the levels of triglyceride and cholesterol, low density lipoprotein effect in the liver and serum of the tested hamsters.
封面內頁
簽名頁
授權書iii
中文摘要iv
英文摘要vi
誌謝viii
目錄ix
圖目錄xi
表目錄xiii
1. 前言1
2. 文獻回顧3
2.1 月桃之簡介3
2.2 月桃之生理活性及機能性成分3
2.3 月桃之抗氧化能力6
2.4 月桃精油成分分析及化學結構的鑑定7
2.5 高脂飲食7
2.5.1 高脂飲食對血脂之影響8
2.5.2 高脂飲食與動脈粥狀硬化(Atherosclerosis)之探討8
2.5.2.1 動脈粥狀硬化(Atherosclerosis)之致病機轉 (pathogenesis)8
3. 月桃種子之一般組成、抗氧化性及芸香苷及槲皮酮之分析12
3.1 摘要12
3.2 前言13
3.3 材料與方法13
3.3.1 實驗材料13
3.3.2 實驗藥品14
3.3.3 實驗設備14
3.4 實驗方法15
3.4.1 一般成分分析15
3.4.2 抗氧化能力試驗15
3.4.3 芸香苷及槲皮酮17
3.5 結果與討論20
3.5.1 月桃種子一般性成分20
3.5.2 月桃種子水萃取物及乙醇萃取物之收率、萃取物總酚及芸香苷及槲皮酮含量20
3.5.3 月桃種子之水及乙醇萃取物之抗氧化性質24
3.6 結論28
4. 月桃種子之揮發性成分分析鑑定30
4.1 摘要30
4.2 前言30
4.3 材料與方法31
4.3.1 實驗材料31
4.3.2 實驗藥品31
4.3.3 實驗設備31
4.4 實驗方法32
4.4.1 精油中組成的定量及定性分析32
4.4.1.2 儀器設備32
4.5 結果與討論34
4.6 結論42
5. 月桃種子降血脂之研究43
5.1 摘要43
5.2 前言43
5.3 材料與方法44
5.3.1 實驗材料44
5.3.2 實驗樣品46
5.3.3 實驗設備47
5.4 實驗方法47
5.4.1 生化分析項目48
5.5 結果與討論53
5.5.1 生長參數54
5.5.2 倉鼠血清及肝臟中脂質之分析59
5.6 結論77
6. 結論78
參考文獻80
附錄89

圖目錄
圖2.1 月桃果實4
圖2.2 月桃種子4
圖3.1 月桃種子水及乙醇萃取物清除DPPH自由基之能力25
圖3.2 月桃種子水及乙醇萃取物對亞鐵離子之螯合能力27
圖3.3 月桃種子水及乙醇萃取物之抗氧化能力29
圖5.1 月桃種子降血脂動物試驗之流程45
圖5.2 餵予月桃種子飲食之倉鼠於誘導及治療期間體重之變化(1)56
圖5.3 餵予月桃種子飲食之倉鼠於誘導及治療期間體重之變化(2) 57
圖5.4 餵予月桃種子飲食對倉鼠相對肝臟重量之影響58
圖5.5 餵予月桃種子飲食對倉鼠血清中三酸甘油酯濃度之影響60
圖5.6 餵予月桃種子飲食對倉鼠血清中總膽固醇濃度之影響62
圖5.7 餵予月桃種子飲食對倉鼠血清中低密度脂蛋白膽固醇濃度之影響65
圖5.8 餵予月桃種子飲食對倉鼠血清中高密度脂蛋白膽固醇濃度之影響67
圖5.9 餵予月桃種子飲食對倉鼠血清中低密度脂蛋白膽固醇/高密度脂蛋白膽固醇比例之影響69
圖5.10餵予月桃種子飲食對倉鼠肝臟中三酸甘油酯之影響70
圖5.11 餵予月桃種子飲食對倉鼠肝臟中總膽固醇之影響72
圖5.12 餵予月桃種子飲食對倉鼠肝臟中磷脂質之影響74

表目錄
表 3.1 月桃種子中芸香苷與槲皮酮分析之HPLC沖提程式19
表3.2 月桃種子之ㄧ般組成分分析21
表3.3 月桃種子水萃物及乙醇萃取物之收率22
表3.4 月桃種子之水及乙醇萃取物中總酚、芸香苷及槲皮酮含量23
表4.1 月桃種子精油之揮發性化合物百分組成之比較36
表5.1 月桃種子粉及精油實驗飼料組成49
表5.2 餵予月桃種子於不同誘導期及治療期間各組倉鼠每日每隻攝食量之變化55
表5.3 餵予月桃種子飲食對倉鼠肝臟中游離脂肪酸之影響76
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