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研究生:陳貞伃
研究生(外文):Chen,Jen-Yu
論文名稱:應用分子印模改善胜肽庫對氣味分子篩選之研究
論文名稱(外文):Research of Peptide Library for Improving Olfaction Screen by Molecular Imprinting
指導教授:高華生高華生引用關係
指導教授(外文):Gao,Heau-Shan
學位類別:碩士
校院名稱:輔仁大學
系所名稱:化學系
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:103
中文關鍵詞:胜肽庫分子印模感測器丁酸位置篩選法
外文關鍵詞:peptide librarymolecular imprintingsensorbutyric acidpositional scanningodorant
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  應用組合化學的概念製備胜肽庫,針對所有相同長度胜肽進行篩選,找出與目標氣味分子有特異性吸附的序列。考量胜肽構形影響,引入分子印模(molecular imprinting)的理念,試圖固定胜肽在吸附目標氣味分子狀態下的特殊活性構形。藉助生物感測器──多頻道氣相微天秤作為檢測工具,探討胜肽和目標氣味分子間吸附效能。
  實驗結果證明,胜肽與目標氣味分子間的印模效應將強弱吸收值差距拉大,並協助彰顯胜肽吸附特性,增加檢測靈敏度。此印模胜肽庫,應用在氣相生物感測器上,不需藉助高分子定型可有良好特異性表現,且有助提高篩選效率。
  This research is looking for the peptides which absorb the target odorant from combinatorial peptide library. The conformations of peptides are considered as one of the key factors for absorption effect. Therefore, molecular imprinting was introduced to enhance the conformations of peptides.
  The result showed the imprinted molecular holes would improve the absorption effect between butyric acid and peptides as well as differentiate well-absorbed samples from poor-absorbed ones. Besides, the imprinted peptides showed higher sensitivity than non-imprinted peptides. It was indicated that the imprinted peptides enhanced specificity and sensitivity. The imprinted peptide library can be used in gas-phase biosensor without polymer formalization.
第一章 緒論 1
第二章 研究背景 5
第一節 目標氣體分子──丁酸 6
第二節 胜肽庫的功用 8
第三節 石英晶體微天秤偵測概念 22
第四節 分子印模技術 26
第三章 研究方法 31
第一節 化學混合法暨固相合成法製備胜肽庫 32
第二節 幫丁酸分子印模 42
第三節 位置篩選法挑選候選胺基酸 46
第四節 印不印模有關係嗎? 47
第四章 材料和儀器 49
第五章 實驗結果 55
第一節 胜肽庫產重、產率 56
第二節 挑選規則及篩選結果與討論 60
第三節 印模相較非印模結果 69
第四節 長期穩定度測試 86
第六章 結論 89
第七章 參考文獻 93
第八章 附錄(原始數據) 101
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