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研究生:江幸芸
研究生(外文):Hsing Yun Chiang
論文名稱:異黃酮的genistein對四氯化碳誘發小鼠肝損傷之護肝效果
論文名稱(外文):Studies of genistein on hepatoprotective effect in CCl4-induced liver-damaged mice
指導教授:盧義發盧義發引用關係
學位類別:碩士
校院名稱:輔仁大學
系所名稱:營養科學系
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:135
中文關鍵詞:異黃酮四氯化碳肝損傷奈米化抗氧化
外文關鍵詞:genisteincarbon tetrachlorideliver-damagenanoparticleantioxidation
相關次數:
  • 被引用被引用:1
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  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
許多文獻指出四氯化碳 (carbon tetrachloride; CCl4) 會造成肝損傷。異黃酮中的 genistein 具有抗氧化的功能。因此想了解 genistein 是否能改善 CCl4 所誘發肝損傷造成的影響,並且探討 genistein 奈米化後是否提高其功能。實驗在 0 小時給予六週大的 C57BL/6J 雄性小鼠不同劑量 (5, 10, 20 及40 mg/kg BW) genistein 或奈米化 genistein (20, 40 mg/kg BW ),第 2 小時腹腔注射 1 mL/kg BW CCl4 誘發肝損傷, 24 小時後犧牲,取血液、肝臟進行生化分析。結果發現隨著 genistein 劑量的增加,可降低肝損傷小鼠血漿中的天門冬胺酸轉胺酶 (aspartate aminotransferase; AST) 、丙胺酸轉胺酶 (alanine aminotransferase ; ALT) 活性;在抗氧化酵素活性方面,可增加過氧化氫酶活性,及超氧岐化酶活性,並增加麩胱甘肽硫轉移酶的活性,尤其在高劑量下,可增加肝臟中總抗氧化能力及麩胱甘肽 (glutathione; GSH) 的含量,並提高 GSH/GSSG 的比值。與未奈米化的genistein 比較,給予奈米化 genistein 的小鼠有顯著降低 ALT 及 降低 AST 的趨勢,並可顯著增加肝臟的總抗氧化力與 GSH 含量及降低 TBARS (thiobarbituric acid reactive substances) 。以上結果,推測 genistein 可減緩四氯化碳造成小鼠的肝損傷;而奈米化 genistein 可以達到更佳的減緩肝損傷效果。
Many researches showed that CCl4 can cause acute or chronic liver-damage and also increase oxdative stress. The genistein of isoflavones is potentially antioxidative function. Therefore, the effects of genistein on hepatoprotective effect or its nanoparticle powder on reducing damage liver were investigated in this study. Following the oral supplementation of genistein (5, 10, 20 and 40 mg/kg BW) or its nanoparticle (20 or 40 mg/kg BW), mice were treated CCl4 (1 mL/kg) at the second hour by intra-peritoneal injection, and then sacrificed at 24th hours. Result showed that the genistein could lower activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in liver damaged mice with increase dose. In antioxidative enzymes, catalase, superoxide dismutase and glutathione S-transferase (GST) decrease to the level of control group in genistein treated mice. Trolox equivalent antioxidant capacity (TEAC), glutathione (GSH) and GSH/GSSG in liver increased with the genistein increase. Nanoparticle of genistein had a trend of lowered activities of AST and ALT, while higher TEAC and GSH, and lower TBARS were found in nano group. The results indicate that genistein were able to diminish the toxic of CCl4. Moreover, nanoparticle of genistein have a better effect than un-nano one.
目錄--------------------------------------------------------------------------------Ⅰ
表目錄-----------------------------------------------------------------------------Ⅳ
圖目錄-----------------------------------------------------------------------------Ⅴ附表目錄-------------------------------------------------------------------------Ⅵ
附圖目錄-------------------------------------------------------------------------Ⅵ
中文摘要--------------------------------------------------------------------------Ⅷ
英文摘要--------------------------------------------------------------------------Ⅸ
致謝--------------------------------------------------------------------------------Ⅹ
壹、緒言---------------------------------------------------------------------------1
貳、文獻回顧---------------------------------------------------------------------3
一、自由基與活性氧---------------------------------------------------------3
(一) 自由基的定義---------------------------------------------------------3
(二) 自由基的來源---------------------------------------------------------3
(三) 氧化壓力與疾病------------------------------------------------------4
二、生物體內抗氧化系統----------------------------------------------------5
(一) 生物體內解毒系統---------------------------------------------------5
1. 細胞色素 P450 系統-----------------------------------------------5
2. 麩胱甘肽硫轉移酶--------------------------------------------------6
(二) 抗氧化防禦系統------------------------------------------------------7
1. 酵素性抗氧化系統--------------------------------------------------8
2. 非酵素性抗氧化系統-----------------------------------------------9
三、大豆異黃酮素-----------------------------------------------------------12
(一) 大豆異黃酮---------------------------------------------------------12
(二) 異黃酮之結構-----------------------------------------------------12
(三) 異黃酮之抗氧化能力--------------------------------------------13
(四) 異黃酮素中之 genistein ----------------------------------------14
四、肝臟----------------------------------------------------------------------16
(一) 肝炎-----------------------------------------------------------------16
(二) 肝發炎指數--------------------------------------------------------16
五、四氯化碳----------------------------------------------------------------18
(一) 性狀與毒性--------------------------------------------------------18
(二) 對肝臟發炎指數相關酵素活性的影響-----------------------19
(三) 對抗氧化相關指標的影響--------------------------------------20
(四) 不同抗氧化物對四氯化碳肝損傷的保護作用--------------21
六、奈米----------------------------------------------------------------------22
(一) 奈米之介紹--------------------------------------------------------22
(二) 奈米科技與食品--------------------------------------------------23
(三) 奈米化 genistein--------------------------------------------------25
參、材料與方法-----------------------------------------------------------------26
一、實驗目的---------------------------------------------------------------26
二、實驗設計與架構------------------------------------------------------26
三、實驗材料---------------------------------------------------------------28
(一) 動物品種------------------------------------------------------------28
(二) 分析用試藥---------------------------------------------------------28
(三) 儀器設備------------------------------------------------------------31
四、實驗方法----------------------------------------------------------------32
(一) 實驗 I - 異黃酮對四氯化碳誘發肝損傷的保護作用------32
(二) 實驗 II - 奈米化與非奈米異黃酮對四氯化碳誘發肝損傷的保護作用之比較------------------------------------------------------42
(三) 統計方法------------------------------------------------------------46
肆、結果--------------------------------------------------------------------------47
實驗 I--------------------------------------------------------------------------47
一、對體重與肝臟重量的影響-----------------------------------------47
二、血漿生化值的影響--------------------------------------------------47
三、血漿及肝臟中總抗氧化力與脂質過氧化的影響--------------48
四、血漿及肝臟中抗氧化酵素活性影響------------------------------48
五、血漿及肝臟中 glutathione S-transferase (GST) 活性影響---49
六、肝臟中麩胱甘肽 (GSH) 及 genistein 含量的影響-----------49
實驗 Ⅱ------------------------------------------------------------------------50
一、對體重與肝臟重量的影響------------------------------------------50
二、血漿生化值的影響---------------------------------------------------50
三、血漿及肝臟中總抗氧化力與脂質過氧化的影響----------------50
四、血漿及肝臟中 glutathione S-transferase (GST) 活性影響----51
五、肝臟中麩胱甘肽 (GSH) 及 genistein 含量的影響------------51
伍、討論--------------------------------------------------------------------------53
陸、結論--------------------------------------------------------------------------64
柒、參考文獻--------------------------------------------------------------------84
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