臺灣博碩士論文加值系統

本研究係利用微弧氧化(micro-arc oxidation, MAO)處理，將鈦基材上分別披覆上一層氧化鈦鍍層與鈣磷鍍層，並在表面上產生不同大小的孔洞。目的是為了探討不同孔洞大小的表面結構對細胞活性之影響。X-ray繞射儀與EDS鑑定披覆鍍層的相組成及成分，FE-SEM觀察經MAO處理後的表面形態與厚度。結果發現鍍層分為氧化鈦鍍層與鈣磷鍍層兩種，在電壓300 V時，其孔洞大小約為0.1 μm∼1 μm之間；而在電壓400 V時，則約為0.5 μm∼2 μm之間。厚度隨著反應時間增加而增加，一分鐘增加約1 μm∼1.5 μm。親水性方面，經MAO處理後的試片，皆有非常良好的親水性。細胞相容性方面，將人類骨肉瘤細胞分別培養於基材上，探討其生物反應。利用細胞增生測試(Methylthiazoletetrazolium assay, MTT assay)細胞培養於試片1, 3, 5與10天的增生能力，細胞在B41(鈣磷鍍層，400 V, 1 min)的增生能力最好，生長最為迅速。並測試細胞分生能力(alkaline phosphate activity assay, ALP assay)，B41試片相對於其他試片有較好的分生能力。FE-SEM觀察細胞培養0.5, 1, 3與12小時的細胞形態，發現細胞在B41試片基材的適應能力最好，表示B41試片有較好的細胞親和性。上述結果顯示孔洞大小的確會影響細胞的形態與反應，孔洞較大的表面確實有較好的細胞反應。

Ca-P (calcium phosphate) ceramics are bioactive materials. We used micro-arc oxidation (MAO) to manufacture TiO2 and Ca-P porous coatings on titanium substrates. The aim of this study was to investigate the properties of two coatings on the cell response. We used three instruments to investigate the coatings, including X-ray diffraction, FE-SEM and EDS. The results showed that we fabricated two different films with TiO2 and Ca-P. The Ca-P films were deposited about 1.7∼5.3 μm. The porous scale of Ca-P films were 0.1 μm∼1 μm at 300 V and 0.5 μm∼2 μm at 400 V. By sessile drop technique, all specimens showed hydrophilic property. In addition, coatings were evaluated with HOS (human osteosarcoma) which incubated on samples. Results in the cell proliferation, the B41 (Ca-P film, 400 V, 1 min) exhibited the best biocompatibility. Sample B41 exhibited more ALP activity than other samples. In the cell morphology, osteoblasts had the faster rate accreted on B41. In conclusion, it was showed that the big porous structure affects cell behaviors.

中文摘要 I
Abstract II
目 錄 IV
表 目 錄 VII
圖 目 錄 VIII
第一章、緒論 1
1-1、前言 1
1-2、研究動機 1
1-3、骨科生醫材料之分類 2
1-3-1、生物活性材料 3
1-3-2、生物可吸收材料 3
1-3-3、生物惰性材料 3
1-3-4、生物可忍受材料 4
1-4、披覆技術簡介 4
1-4-1、電漿熔射 4
1-4-2、離子濺鍍 4
1-4-3、生物礦仿化法 4
1-4-4、電化學沉積法 5
1-4-5、陽極化處理 5
1-5、研究目的 5
第二章、理論基礎 6
2-1、鈦與鈦合金基本性質 6
2-2、氧化鈦基本性質 6
2-3、磷酸鈣基本性質 8
2-4、陽極化處理與其應用 8
2-4-1、微弧氧化技術 9
第三章、實驗流程與研究方法 10
3-1、研究方法 10
3-1-1、實驗流程 10
3-1-2、實驗材料 10
3-2、試片前處理 10
3-3、微弧氧化陽極處理裝置 11
3-4、基本性質測試 11
3-5、接觸角測試 11
3-6、體外實驗 11
3-6-1、滅菌處理 12
3-6-2、細胞培養 12
3-6-3、細胞增生活性測試 12
3-6-4、細胞分生活性測試 13
3-6-5、細胞表面形態觀察 14
3-6-6、統計分析 15
第四章、結果與討論 16
4-1、基本性質探討 16
4-1-1、XRD分析 16
4-1-2、表面形態分析 17
4-1-3、橫截面分析 18
4-1-4、接觸角分析 19
4-2、體外實驗 19
4-2-1、細胞增生實驗分析 19
4-2-2、細胞分生實驗分析 20
4-2-3、細胞表面形態分析 21
第五章、結論 23
參考文獻 24

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