臺灣博碩士論文加值系統

在藥物使用的範疇中，如果想要使藥品能夠完全的發揮出療效，藥物的控制與釋放一向是研究的重點；並且在過去的研究文獻中發現，藥物載體的粒徑越均一，則在應用於藥物的控制與釋放上就越有優勢，但在過去文獻中所提出的製作方式，均無法有效獲得均一粒徑的載體。本研究設計了三組流道形狀和利用翻模技術製作PDMS微流道晶片，用於生成均一粒徑乳化球，在研究中設計了十字型、T字型 (type 1和type 2)三種不同微流道晶片，希望可以生成均一粒徑的乳化球，並應用於生成均一粒徑光交聯微粒子的製備上。本研究成功利用所設計的PDMS微流道晶片設備，生成均一粒徑乳化球，並且應用在光交聯微粒子上，並且探討了分離相與連續相的流量改變與生成之乳化球大小之關係，粒徑分布大小在40 ~1000 µm之間。由實驗結果中發現，當固定分離相流量時，越大的連續相流量，將得以生成越小的乳化球；相對地，在固定連續相流量的情形下，當分離相的流量越小，所生成的乳化球尺寸也越小。

In this paper the manipulation of UV-polymerizable microparticles, using a microfluidic chip, for the encapsulation of gold nanoparticles is presented. Our strategy is based on hydrodynamic-focusing on the forming of a series of self-assembling sphere structures, the so-called water-in-oil (W/O) emulsions, in the cross-junction microchannel and T-junction microchannel. We have demonstrated that one can control the size of UV-polymerizable microparticles from 40 µm to 1000 µm in diameter (with a variation less than 10%) by altering the relative sheath/sample flow rate ratio. The microfluidic chip is capable of generating relatively uniform micro-droplets and has the advantages of active control of droplet diameter, simple and low cost process, and high throughput.

摘要 III
Abstract IV
誌謝 V
圖目錄 X
第一章 緒論 1
1-1 藥物控制釋放系統的必要性 3
1-2 藥物載體之光交聯水膠材料介紹 4
1-2-1 光交聯反應原理 6
1-2-2 製備光交聯水膠的方法 9
1-2-3 光交聯水膠的應用 10
1-3 文獻回顧 11
1-4 研究動機與目的 18
1-5 實驗架構 19
第二章 微流道晶片設計製作與數值模擬 20
2-1 微流道晶片設計 (十字型、T字型(type 1和type 2)) 20
2-2 PDMS微流道晶片製作方法 24
2-2-1 PMMA母模製作方法 (十字型晶片) 30
2-2-2 EPOXY母模製作方法 (T字型晶片(type 1和type 2)) 33
2-2-3 PDMS微流道晶片的製作 36
2-3 晶片接合技術 46
2-3-1 熱壓接合 46
2-3-2 氧電漿系統接合 46
2-4 數值模擬與分析 50
2-4-1 CFD-RC軟體介紹 50
2-4-1-1 CFD-GEOM部分 51
2-4-1-2 CFD-ACE(U)部份 52
2-4-1-3 CFD-VIEW部分 53
2-4-2 網格密度收斂性 54
2-4-2-1 十字型晶片 55
2-4-2-2 T字型晶片(type 1) 56
2-4-2-3 T字型晶片(type 2) 58
2-4-3 微流道晶片模擬參數設定 59
2-4-3-1 十字型晶片 59
2-4-3-2 T字型晶片(type 1 and type 2) 60
第三章 實驗與研究方法 61
3-1 實驗設備介紹 61
3-1-1 晶片製作設備 61
3-1-2 微量注射幫浦儀器 66
3-1-3 即時觀測系統 67
3-1-4 乳化球生成平台 68
3-1-5 紫外燈照射儀器 68
3-1-6 紫外光-可見光吸收光譜儀 69
3-1-7 黏滯係數量測系統 70
3-1-8 真空幫浦系統 71
3-2 實驗藥品介紹 72
3-2-1 光交聯乳化球實驗 72
3-2-2 金奈米粒子包覆實驗 72
3-3 實驗方法 74
3-3-1 十字型晶片生成光交聯微粒子實驗 75
3-3-2-1 T字型晶片(type 1) 76
3-3-2-2 T字型晶片(type 2) 77
3-3-3 光交聯微粒子包覆金奈米粒子 78
第四章 結果與討論 79
4-1 模擬分析結果 80
4-1-1 十字型晶片 80
4-1-2 T字型晶片(type 1) 81
4-1-3 T字型晶片(type 2) 83
4-2 流量與光交聯微粒子生成粒徑之關係 85
4-2-1 十字型晶片 85
4-2-2 T字型晶片(type 1) 91
4-2-3 T字型晶片(type 2) 97
4-3 金奈米粒子包覆實驗 103
第五章 結論與建議 105
5-1 結論 105
5-2 建議 107
參考文獻 108

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