臺灣博碩士論文加值系統

γ-次亞麻油酸為一高經濟價值的不飽和脂肪酸，主要來源是萃取自高等植物，而高等植物生產不飽和脂肪酸的速度較慢且不易品質管控，所以本研究利用油脂性微生物以饋料醱酵方式來探討大量生產γ-次亞麻油酸。
有機氮源被廣泛使用在醱酵系統中，但其缺點是無法分析與測量，故研究過程中必須利用大量的實驗結果，確定其適合的培養濃度與饋料濃度，造成研究成本提升。所以在本研究中利用數學模型進行研究，進而趨近氮源在系統內消耗之情況。期望透過此方法的建立，可以廣泛應用於需要決定氮源濃度隨時間變化的醱酵系統中，更進一步，以該數學模型做為演算出饋料策略的依據，用以提高生產效益。
利用數學模型作為擬定饋料策略的依據，本研究以得到最高總油脂產率為其目標，並固定葡萄糖濃度，進行演算饋料策略，可以得到初始濃度與饋料策略分別為：初始濃度為葡萄糖16.904克/升和玉米粉1.707克/升，饋料濃度為葡萄糖2.607克/升和玉米粉0.016克/升，培養時間18.51小時可得到總油脂為3.659克/升及γ-次亞麻油酸0.366克/升。

γ-linolenic acid(GLA) was a value-added polyunsaturated fatty acids(PUFA). its main source was extract form plants, but producing GLA by plants was slow and quality of GLA can’t be control. Therefore, this research was production by fermentation.

Organic nitrogen source was generally used for industrial fermentation, but it couldn’t measure and analysis easily. Therefore, we needed a lot of data to find out optimal concentration of culture and fed. This result makes high cost of research. In this research, we used mathematic model to investigate and estimate the concentration of organic nitrogen source. We hoped to establish this method can be generally used to find out the concentration of nitrogen source for fermentation. Future, the mathematic model can be used for drafting strategy of fed to increase cost-effective.

According model to drafting a strategy of fed and the object is get high yield and constant glucose concentration, we get a strategy which was initial concentration: glucose16.904g/L and corn steep solid 1.707g/L, fed concentration: glucose 2.607g/L and corn steep solid 0.016g/L, and producing total lipid 3.659g/L and GLA 0.366 g/L after 18.51 hrs.

第一章 緒論 11
1.1前言 11
1.2 研究目的 14
1.3 研究動機 15
1.4 多元不飽和脂肪酸與γ-次亞麻油酸 15
1.4.1 多元不飽和脂肪酸 15
1.4.2 不飽和脂肪酸的應用 18
1.4.3 γ-次亞麻油酸（γ-Linolenic acid，GLA） 19
1.5 利用微生物生產多元不飽和脂肪酸的優勢 22
1.5.1 多元不飽和脂肪酸之微生物條件 23
1.5.2 油質性真菌 Mucor rouxii 25
1.5.3 微生物產油機制 26
1.6 油脂性微生物之培養 29
1.7 微生物生長動力學之數學模型 32
1.8 論文之章節 33
第二章 研究方法與材料 34
2.1 研究材料 34
2.1.1 菌株來源 34
2.1.2 培養基 34
2.1.3 實驗設備 38
2.2研究方法 39
2.2.1 菌種保存 39
2.2.2 前培養條件 40
2.2.3 批次培養探討 40
2.2.4 饋料時間探討 41
2.2.5 饋料濃度探討 41
2.3 分析方法 42
2.3.1 菌體量(biomass)分析方法 42
2.3.2 葡萄糖分析方法 42
2.3.3 脂質萃取與定義方法 43
2.3.4 脂肪酸分析條件 46
2.4 微生物生長模型 47
2.4.1 模型的建立與定義 47
2.4.2 饋料模型的建立與定義 49
2.5狀態變數(state variable) 51
第三章 實驗結果與討論 53
3.1 Mucor rouxii 之脂肪酸分析結果 53
3.2 批次實驗 55
3.2.1不同碳氮濃度比培養基對微生物生長影響之探討 55
3.2.2 不同饋料時間對生長狀態的探討 60
3.3 不同饋料濃度對於生長狀態的影響 63
3.4生長動力學模型 66
3.4.1 參數估計 66
3.4.2 參與參數估之實驗數據 67
3.4.3 模型驗證 78
3.5 饋料批次策略 81
第四章 結論與未來展望 86
4.1 結論 86
4.2 未來展望 87
參考文獻 88

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沈建緯(2007)以批次培養探討Mucor rouxii 生產γ-次亞麻油酸之研究 成功大學化學工程系

陳德偉(2004)基因重組大腸桿菌醱酵系統模式建立與最適化 中正大學化學工程系

臧荣春,夏凤毅(2004) 微生物動力學模型 化學工業出版社 現代生物技術與醫藥科技出版中心