臺灣博碩士論文加值系統

國軍食勤人員油煙中多環芳香烴的暴露與體內氧化壓力之關係

軍隊為集體生活之團體，食勤人員每餐需準備相當可觀之伙食，因而食勤作業人員可能暴露於大量的油煙中，而油煙中含有大量的有毒物質，其中包含大量之多環芳香烴(Polycyclic aromatic hydrocarbons, 簡稱PAHs)，其會增加體內的自由基，而使人體氧化壓力上升，造成DNA的氧化性傷害。
本研究目的乃利用縱斷性研究了解五日油煙PAHs暴露後的生物標記1-OHP的變化量及油煙暴露後造成體內氧化性壓力指標8-OHdG的變化，且進一步了解油煙PAHs暴露與氧化壓力之關係。研究選取北、中、南部各一個大型營區中所有食勤作業人員77員及單位人數配對選取之一般士兵為對照組76員，收取第一日工作前與第五日工作後之尿液分析1-OHP與8-OHdG，及利用健康問卷、每日暴露問卷及收尿前24小時問卷了解基本人口學資料、平時工作狀況及追蹤期間相關干擾因子或食勤作業的暴露狀況。
結果顯示，食勤作業人員尿中1-OHP濃度明顯上升(0.305上升至0.638 µg/g-creatinine），且在GEE模式中，控制吸菸等因素下，食勤作業人員上升量顯著比對照組高；食勤人員尿中8-OHdG濃度則出現下降趨勢(8.58下降至7.71 µg/g-creatinine)，而在GEE模式中，控制吸菸等因素下，食勤人員與對照組下降趨勢並無顯著差異；尿中1-OHP濃度與8-OHdG濃度亦無明顯相關，且進一步利用GEE模式分析發現，1-OHP的變化並不會影響到8-OHdG的變化。
因此，1-OHP可當作食勤人員暴露油煙PAHs後的良好生物標記，反之，尿中8-OHdG並未能即時反應出食勤人員的氧化壓力狀況，因此，有待爾後研究能進一步發現更良好的氧化壓力指標。而食勤人員會暴露於大量油煙PAHs中，應強化其防護觀念之認知與執行，以確保食勤人員之健康。

The military cooks had heavy burden of cooking work, they may expose to cook oil fumes in military kitchens. Cook oil fumes (COF) constituted a complex mixture of toxicants, including polycyclic aromatic hydrocarbons(PAHs) which may induce oxidative DNA damage. The purpose of this study was to study the relationships between COF exposure and the oxidative DNA damage.
We carried out a longitudinal study to explore the relationships. Exposure assessment based on a biomarker of PAHs exposure, urinary 1-hydroxypyrene（1-OHP）. Urinary 8-hydroxydeoxyguanosine (8-OHdG) was used as a biomarker of oxidative DNA damage. Study subjects were recruited from 3 military bases of the north, middle and southern part in Taiwan. The exposed group included 77 male military cooks exposed to COF. The control group consisted of 76 office soldiers not exposured to COF during the study period.
We distributed a self-administrated questionnaire to the subjects, which inquire about personal characteristrics, such as age, education, smoking habits, histories of disease, consumption of broiled, grilled and barbecued food,and use of personal protective equipment. Workers were asked to collect a pre-shift of the first weekday and a post-shift of the fifth workday urine samples.
The results showed that urinary 1-OHP of military cooks increased significantly (0.305→0.638 µg/g-creatinine) after 5 days exposure to COF. Multivariate GEE alalysis indicated military cooks has statistically significant elevated levels of 1-OHP than that of control group. On the other hand, urinary 8-OHdG of military cooks decreased (8.58→7.71 µg/g-creatinine) from pre-shift of the first day of workweek to post-shift of the last day of workweek. Multivariate GEE alalysis showed military cooks had no significant decreasing levels of 8-OHdG compared with control group. Spearman correlation analysises showed urinary 1-OHP was not correlated with urinary 8-OHdG. Multivariate GEE alalysis indicated that the change of urinary 1-OHP with time didn’t affected the change of urinary 8-OHdG.
Our results demonstrated that urinary 1-OHP could be a promising biomark of PAHs exposure to COF, whereas urinary 8-OHdG was not associated with 1-OHP to reflect oxidative DNA damage in military cooks.

內文目錄
表目錄 III
圖目錄 V
附錄目錄 VI
縮寫表 VII
中文摘要 VIII
Abstract X
第一章 緒論 1
第一節 研究背景 1
第二節 研究動機及重要性 3
第三節 研究目的 5
第二章 文獻探討 6
第一節 多環芳香烴簡介 6
第二節 油煙的健康危害 12
第三節 多環芳香烴暴露的生物標記 19
第四節 多環芳香烴造成氧化壓力 23
第五節 氧化壓力指標-8-hydroxydeoxyguanosine 26
第三章 研究方法與步驟 30
第一節 研究設計與架構 30
第二節 研究假設 32
第三節 研究對象 33
第四節 研究工具 34
第五節 實驗品質管制 39
第六節 資料收集方法與步驟 42
第七節 資料處理與統計方法 45
第四章 研究結果 48
第一節 橫斷性研究對象基本資料描述性檢定 48
第二節 尿液中1-OHP濃度之分析 50
第三節 尿液中8-OHdG濃度之分析 58
第四節 邏輯式迴歸分析影響尿中1-OHP相關因子 63
第五節 邏輯式迴歸分析影響尿中8-OHdG相關因子 65
第六節 線性迴歸分析影響尿中1-OHP與8-OHdG濃度的相關因子 67
第七節 縱斷性分析尿中1-OHP與8-OHdG的變化 71
第五章 討論 78
第一節 研究對象選取與實驗品質管制 79
第二節 尿中1-OHP濃度變化 80
第三節 尿中8-OHdG濃度變化 87
第四節 尿中1-OHP與8-OHdG相關性探討 91
第六章 結論 95
第七章 建議 96
參考文獻 97

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