跳到主要內容

臺灣博碩士論文加值系統

(34.236.36.94) 您好!臺灣時間:2021/07/24 22:17
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:馮芳蘋
研究生(外文):Feng, Fang-Ping
論文名稱:利用細胞培養的方式觀察LED光處理後破骨細胞的反應
論文名稱(外文):LED Phototreatment on Osteoclast in vitro
指導教授:林清亮林清亮引用關係史中
指導教授(外文):Lin, Chin-LiangShis, Chung
學位類別:碩士
校院名稱:國防醫學院
系所名稱:生物及解剖學研究所
學門:生命科學學門
學類:生物訊息學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:52
中文關鍵詞:破骨細胞LED光
外文關鍵詞:osteoclast
相關次數:
  • 被引用被引用:2
  • 點閱點閱:193
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
中文摘要

根據先前學者們的研究,雷射光的照射在動物體的實驗中可以影響骨組織的代謝情形,然而,LED光源對骨組織的影響目前還不清楚,本篇研究的主要目的是利用體外培養的方式建立一套骨吸收的模式,觀察LED光處理之後對於破骨細胞的影響情形。破骨細胞培養在放有牛緻密骨骨片的24-well培養基中培養,將三至七天大的紐西蘭大白兔犧牲後,取出四肢的長骨,剪碎後分離出破骨細胞,培養在培養基中,在第三天時,將培養的破骨細胞分成對照組與給予630 nm波長的LED光源照射組,在第六天時,使用4%的paraformaldehyde將細胞固定三十分鐘,以TRAP staining kit染色,測量分析二個組別的破骨細胞數量、細胞形態與吸收小凹的數量、面積及移動能力。結果:在細胞數量上,與對照組相比有較多的情形,且具有意義。在細胞形態學的分析上,LED光照射組與對照組相比,明顯有較多的含核數,細胞也較大。LED光照組的細胞在外型上較不規則,這樣的情形說明了LED光照射增加了破骨細胞融合的能力。在破骨細胞活性的分析上,在吸收小凹數量的分析上,LED光照組有較多的數量且具有統計意義,這點顯示LED的照射可增加破骨細胞移動的能力。
結論,LED光處理之後,增進了破骨細胞的形成與活性的增加,包括了骨吸收、移動及融合的能力。
Abstract

According to previous studies, bone metabolism in vivo was affected with several of laser treatments. However the results with another light source (LED) were unclear. The aim of this study plan is to establish a bone resorption model in vitro to determine the effects of phototreament of LED on osteoclasts. Osteoclasts were plated on 24-well culture dish with bovine cortical bone slices. Four limbs bones of three to seven days old New Zealand white rabbits were collected after sacrified. Osteoclasts will be isolated from four limbs bony fragments and plated on culture dishes. On third day, these cultured osteoclasts were irradiated with high power light source - LED (630 nm) beam with two energy released modes (control and continues mode). On sixth day, osteoclasts were fixed with 4% paraformaldehyde for 30 mins then stained with TRAP staining kit. Analysis was determinated by measuring the numbers, cellular morphology of osteoclasts and the numbers and area of resorption pits and migration rate between the two groups. It was showed that LED treatment significantly increased in cell count of multinuclear osteoclasts. In cellular morphology analysis, LED group seemed to have more mean nucleus number and bigger size of the osteoclast than that of the control group. LED treatment group had more irregular morphological shape osteoclast than the control group. It seemed that LED treatment would increase osteoclast fusion ability. In osteoclast activity analysis, LED group significantly increased in resorption pits and area. LED treatment seemed to increase osteoclast migration rate.
In conclusion, LED phototreatment increased osteoclast formation and activity, including resorption, migration and fusion ability.
目錄
頁次
目錄……………………………………………………………………..Ι
中文摘要………………………………………………………………II
英文摘要………………………………………………………………V
第一章 緒言……………………………………………………………..1
 第一節 骨組織……………………………………………………..1
壹、骨組織結構…………………………………………….1
貳、骨組織形成與在重塑作用 (bone remodeling)……….2
第二節 破骨細胞…………………………………………………3
壹、破骨細胞……………………………………………..3
貳、破骨細胞功能…………………………………………..6
一、骨吸收……………………………………………6
二、移動與融合……………………………………7
第三節 光子生物學 (photobiology)……………………………8
 壹、雷射作用在皮膚組織…………………………………8
貳、雷射作用在骨組織……………………………………..9
参、雷射作用在細胞…………………………………….10
 一、成骨細胞……………………………………………10
 一、破骨細胞……………………………………………10
参、雷射使用的缺點……………………………………….12
第四節 LED光對生物體的刺激效應…………………………….12
壹、LED生物醫學的應用………………………………13
貳、LED在活體組織與細胞的應用…………………………13
第五節 研究目的……………………….…………………………...15
第二章 材料與方法……………………………………………………16
第一節 實驗材料…………………………………………………16
第二節 實驗方法…………………………………………………17
壹、破骨細胞的取得…………………………………………17
貳、實驗設計…………………………………………………18
第三章 結果……………………………………………………………….19
第一節 破骨細胞數量分析…………………………………..………19
第二節 破骨細胞活性分析……………………………….…..23
第四章 討論…………………………………………………………28
第五章 結論……………………………………………………………33
參考文獻………………………………………………………………..34
參考文獻
Abergel RP, Meeker CA, Lam TS, Dwyer RM, Lesavoy MA, Uitto J. Control of connective tissue metabolism by lasers: recent developments and future prospects. J Am Acad Dermatol, 11: 1142-1150, 1984.

Abergel RP, Lyons RF, Castel JC. Biostimulation of wound healing by lasers: experimental approaches in animal models and in fibroblast cultures. J Dermatol Surg Oncol, 13: 127-133, 1987.

Almeida LL, Rigau J, Zangaro RA, Guidugli NJ, Jaeger MMM. Comparison of the low gingival fibroblasts proliferation using different irradiance and same fluence. Laser Surg Med, 29: 179-184, 2001.

Aihara N, Yamaguchi M, Kasai K. Low-energy irradiation stimulates formulates formation of osteoclast-like cells via RANK expression in vitro. Lasers Med Sci, 2005.

Baron R, Neff L, Tran VP, Nefussi JR, Vignery A. Kinetic and cytochemical identification of osteoclast precursors and their differentiation into multinucleated osteoclasts. Am J Pathol 122:363-378, 1986.

Boyle WJ, Simonet W.S, Lacey DL. Osteoclast differentiation and activation. Nature, Vol 423, 2003.

Bruzzaniti A, Neff L, Sanjay A, Horne WC, Camilli PD, Baron R. Dynamin forms a Src kinase-sensitive complex with Cb1 and regulates podosomes and osteoclasr activity. Molecular Biology of the Cell, Vol. 16, 3301-3313, 2005.

Dekel S, Salama R, Edelstein S. The effect of vitamin D and its metabolites on fracture repair in chicks. Clin Sci, 65: 429-436, 1983.

Dierickx CC, Anderson RR. Visible light treatment of photoaging. Dermatol Ther, 18: 191-208, 2005.

Dortbudak O, Haas R, Mallath-Pokorny G. Biostimulation of bone marrow cells with a diode soft laser. Clin Oral Implants Res, 11: 540-545, 2000.

Frost HM. From wolff’s low to the Utah paradigm: insights about bone physiology and its clinical applications. Anatomi Rec, 262: 398-419, 2001.

Gordon SA, Surrey K. Red and far-red light action on oxidative phosphorylation. Radiat Res, 12: 325-339, 1960.

Heckman JD, Rayby JP, McCabe J, Frey JJ, Kilcoyne RF. Acceleration of tibial fracture-healing by non-invasive, low intensity pulsed ultrasound. J Bone Joint Surg, 76-A: 26-34, 1994.

Janckila AJ, Parthasarathy RN, Parthasarathy LK, Seelan RS, Hsueh YC, Rissanen J, Alatalo SL, Halleen JM, Yam LT. Properties and expression of human tartrate-resistant acid phosphatase isoform 5a by monocyte-derived cells. J Leukoc Biol. 2005 Feb;77(2):209-18. Epub 2004 Nov 12.

Kanehisa J, Yamanaka T, Doi S, Turksen K, Heersche JN, Aubin JE, Takeuchi H. A band of F-actin c obtaining podosomes is involved in bone resorption by osteoclasts. Bone 11:287-93, 1990.

Karu I. Molecular mechanism of therapeutic effects of low intensity laser radistion. Lasers Life Sci. 2:53-74, 1988.

Kato M, Shinizawa K, Yoshikawa S. Cytochrome oxidase is a possible photoreceptor in mitochondria. Photobiochem Photobiophys, 2: 263-269, 1981.

Kreisler M, Christoffers A B, Haitham AH, Willershausen B, Hoedt Bd. Low level 809nm diode laser induced in vitro stimulation of the proliferation of human gingival fibroblasts. Laser in Surg & Med, 30: 365-369, 2002.

Li S, Chien S, Branemark PI. Heat shock-induced necrosis and apoptosis in osteoblasts. J Ortho Research, 17:891-899, 1999.

Linder S, Aepfelbacher M. Podosomes: adhesion hot-spots of invasive cells. Trends Cell Biol. 13:376-385, 2003.

Lacey DL, Timms E, Tan HL, Kelley MJ, Dunstan CR, Burgess T, Elliott R, Colombero A, Elliott G, Scully S, Hsu H, Sullivan J, Hawkins N, Davy E, Capparelli C, Eli A, Qian YX, Kaufman S, Sarosi I, Shalhoub V, Senaldi G, Guo J, Delaney J, Boyle WJ. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell 93:165-176, 1998.

Friedmann H, Lubart R, Laulicht I. A possible explanation of laser-induced stimulation and damage of cell cultures. J Photochem Photobiol B: Biol, 11: 87-95, 1991.

Frimer A.A. Singlet oxygen. Physical Chemical Aspects 1, 1985.

Fujii H, Cody SH, Seydel U, Papadimitriou M, Wood DJ, Zheng MH. Recording of mitochondrial transmembrane potential and volume in cultured rat osteoclasts by confocal laser scanning microscopy. Histochemical, 29:571-581, 1997.

Lubart R, Malik Z, Rochkind S, Fisher T. A possible mechanism of low level laser-living cell interaction. Laser Therapy, 2:65-68, 1990.

Lubart R, Wollman Y, Friedmann H, Rochkind S, Laulicht I. Effect of visible and near-infrared laser on cell cultures. J Photochem Photobiol. B: Bio, 12: 305-310, 1992.

Luger EJ, Rochkind S, Wollman Y, Kogan G. Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats. Lasers Surg Med, 22: 97-102, 1998.

Marchisio PC, Cirillo D, Naldini L, Primavera MV, Teti A, Zambonin-Zallone A. Cell-substratum interaction of cultured avian osteoclasts is mediated by specific adhesion structures. J Cell Biol 99:1696-705, 1984.

Masiukiewicz US, Mitnick M, Gulanski BI, Insogna KL. Evidence that the IL-6/IL-6 soluble receptor cytokine system plays a role in the increased skeletal sensitivity to PTH in estrogen-deficient women. J Clin Endocrinol Metab, 87: 2892-2898, 2002.

Mester E, Mester AF, Mester A. The biomedical effects of laser application. Laser application. Lasers Surg Med, 5: 31-39, 1985.

Miyazaki T, Katagiri H, Kanegae Y, Takayangi H, Sawada Y, Yamamotm A, Pando M P, Asano T, Verma IM, Oda H, Nakamura K, Tanaka S. Reciprocal Role of ERK and NF- B pathways in survival and activation of osteoclasts. J cell Biol, 148: 333-342, 2000.

Nagasawa A, Kato K, Negishi A. Bone regeneration effect of low level lasers including argon laser. Laser Ther, 3: 59–62, 1991.

Ozawa Y, Shimizu N, Kariya G, Abiko Y. Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone. 22, 4:347-354, 1998.

Passarella S, Casamassima F, Molinari S, Pastroe D, Quagliariello E, Catalano IM, Cingolani A. Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in virto by He-Ne laser, FEBS Lett, 175: 95-99, 1984.

Pollak D, Floman Y, Simkin A, Avineser A, Freund HR. The effect of protein malnutrition and nutritional support on the mechanical properties of fracture healing in the injured rat. J Parenter Enter Nutr, 10: 564–567, 1986.

Qudri T, Miranda L, Tuner J, Gustaffsson A. The short-term effects of low-level lasers as adjunct therapy in the treatment of periodontal inflammation. J Clin Periodontol, 32: 714-719, 2005.

Quinn JM.W, Gillespie MT. Modulation of osteoclast formation. Biochemi Biophysi Res Communi 328:739-745, 2005.

Riggs BL, Melton LJⅢ. Involutional osteoporosis. J New England Med, 314: 1676-1686, 1986.

Sato M, Grasser W. Effects of bisphosphonates on isolated rat osteoclasts as examined by reflected light microscopy. J Bone Miner Res. 5:31-40, 1990.

Scott G, King JB. A prospective, double-blind trial of electrical capacitive coupling in the treatment of non-union of long bones. J Bone Joint Surg, 74-A: 920–929, 1992.

Sorensen MG, Henriksen K, Schaller S, Henriksen DB, Nielsen FC, Dziegiel MH, Karsdal MA. Characterization of osteoclasts derived from CD14+ monocytes isolated from peripheral blood. J Bone Miner Metab. 2007;25(1):36-45. Epub 2007 Jan 1.

Suda T, Udagawa N, Takahashi N. Cells of bone: osteoclast generation. In: Bilezikian JP, Raisz LG, Rodan GA (eds) Principle of Bone Biology. Academic press, San Diego, pp 87-102, 1996.

Takayanagi H. Mechanistic insight into osteoclast differentiation in osteoimmunology. J Mol Med, 83:170-179, 2005.

Trelles MA, Mayayo E. Bone fracture consolidates faster with low-power laser. Lasers Surg Med, 7: 36–45, 1987.

Vekshin NL, Mironov GP. Flavin-dependent oxygen uptake in mitochondria under illumination, Biophysics (Moscow), 27: 537-539, 1982.

Webb C, Dyson M, Lewis WH P. Stimulatory effect of 660nm low level laser energy on hypertrophic scar-derived fibroblasts: possible mechanisms for increase in cell counts. Lasers Surg Med, 22: 294-301, 1998.

Webb C, and Dyson M. The effect of 880nm low level laser energy on human fibroblast cell numbers: a possible role in hypertrophic wound healing. J Photochem Photobiol. B: Bio, 70: 39-44, 2003.

Weiss RA, McDaniel DH, Geronemus RG, Weiss MA. Clinical trial of a novel non-thermal LED array for reversal of photoaging: clinical, histologic, and surface profilometric results, Lasers Surg Med, 36: 85-91, 2005.

Whelan HT, Houle JM, Whelan NT. The NASA light-emitting diode medical program-progress in space flight and terrestrial application. Space Tech Appl Intl Forum, 552: 35-45, 2000.

Whelan HT, Smits RL, Buchmann EV. Effect of NASA light-emitting diode irradiation on wound healing. J Clin Laser Med Surg, 19: 304-314, 2001.

Whelan HT, Buchmann EV, Dhokalia A, Kane MP, Whelan NT, Wong-Riley MTT, Eells JT, Gound LJ, Hammamieh R, Das R, Jett M. Effect of NASA light-emitting diode irradiation on molecular changes for wound healing in diabetic mice. J Clin Laser Med Surg, 21: 67-74, 2003.

Yasuda H, Shima N, Nakagawa N, Yamaguchi K, Kinosaki M, Mochizuki S, Tomoyasu A, Yano K, Goto M, Murakami A, Tsuda E, Morinaga T, Higashio K, Udagawa N, Takahashi N, Suda T. Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogensis-inhibitory factor and is identical to TRANCE/RANKL. Proc Natl Acad Sci USA 95:3597-3602.

Yamada K. Biological effect of low power laser on clonalosteoblastic cells (MC3T3-E1) . Nippon Seikeigeka Gakkai Zasshi, 65: 787–799, 1991.

阮若屈,郭瑞雄。Effect of visible light on human foreskin fibroblast cells. 中原大學,1981。
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
1. 謝俊義。2000年。〈新制度主義的發展與展望〉。《中國行政》,68期,頁1-26。
2. 蔡增家。2001年。〈金大中金融改革與南韓政經體制的轉變〉。《問題與研究》,5期40卷,頁19-63。
3. 蔡增家。1998年。〈南韓經濟發展的政治經濟分析:1963~1997〉。《問題與研究》,11期37卷,頁29-48。
4. 蔡增家。1998年。〈論國際化對國內政治的影響:以日韓為例〉。《問題與研究》,4期37卷,頁20-35。
5. 蔡增家。2005年。〈九七金融風暴與南韓政經體制的轉變:超越發展國家論〉。《問題與研究》,4期44卷,頁75-100。
6. 鄭端耀。1997年。〈國際關係「新自由制度主義」理論之評析〉。《問題與研究》,第36卷第12期,頁1-22。
7. 顏慶章。2004年。〈WTO與國家發展〉。《今日海關》,32期,頁5-13。
8. 劉大和、蔡靜怡。2002年。〈WTO與APEC服務貿易議題〉。《亞太經濟合作評論》,10期,頁30-37。
9. 蔡宏明。1996年。〈開發中國家與GATT/WTO的關係-兼論我國爭取開發中國家支持之道〉。《今日經濟》,347卷,頁6-10。
10. 陳敦源。2001年。〈新制度論的範圍與方法:一個理性選擇觀點的方法論檢視〉。《行政暨政策學報》,3期,頁129-184。
11. 莊文忠。2003年。〈制度的研究:「新制度論」觀點的比較與「後現代制度論」的發展〉。《理論與政策》,16卷4期,頁15-44。
12. 徐仁輝。1999年。〈制度變遷與美國預算改革〉。《東吳經濟商學學報》,24期,頁89-114。
13. 胡婉玲。2001年。〈論歷史制度主義的制度變遷理論〉。《新世紀智庫論壇》,16期,頁86-95。
14. 林傳琦。2005年。〈韓國稻米政策簡介〉。《農政與農情》,159期396卷,頁67-72。
15. 胡至沛。2001年。〈新制度主義的檢視與反思〉。《中國行政評論》,第11卷第1期,頁145-162。