跳到主要內容

臺灣博碩士論文加值系統

(3.236.84.188) 您好!臺灣時間:2021/07/30 02:41
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:邱后妍
研究生(外文):Hou-Yan Chiou
論文名稱:猪隻表皮生長因子、轉形生長因子α及其嵌合體基因之選殖與表現
論文名稱(外文):Cloning and expression of pig epidermal growth factor, transforming growth factor α and its chimera gene
指導教授:李德南李德南引用關係
指導教授(外文):Der-Nan Lee
學位類別:碩士
校院名稱:國立宜蘭大學
系所名稱:生物技術研究所碩士班
學門:農業科學學門
學類:畜牧學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:85
中文關鍵詞:表皮生長因子嗜甲醇酵母菌轉形生長因子α
外文關鍵詞:epidermal growth factormethotrophic yeasttransforming growth factor α
相關次數:
  • 被引用被引用:0
  • 點閱點閱:150
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
表皮生長因子(Epidermal growth factor, EGF)與轉形生長因子α(Transforming growth factorα, TGFα) 可藉由EGF受體之活化而成為一刺激原,有助於幼小動物建立胃腸黏膜細胞之屏障,而EGF與TGFα與受體間內化程度之不同,故本研究以N結構域和C結構互組產生嵌合體,用以比較EGF、TGFα及其嵌合體之生理活性。經RT-PCR反應選殖出豬EGF、TGFα及ETα嵌合體分子之基因,利用Pichia pastoris菌株配合pPIC9K表現系統生產重組蛋白,其次進行重組蛋白之定性與定量,並以BALB/c 3T3細胞比較其促細胞生長活性。當以發酵槽培養經甲醇誘發表現之重組蛋白,並將發酵液上清液經蛋白質電泳分析,pEGF、pTGFα與pETα分別於6、5.7與5.7 kDa處發現蛋白質的色帶,再以西方墨漬法,更進一步證實其分別可受pEGF、pTGFα與pTGFα抗體辨識,而其重組蛋白之產量約在甲醇誘發後60小時達最高,其pEGF、pTGFα與pETα產量分別約達320、118與27 mg/L。而此三種重組蛋白皆具有促細胞生長之活性,其活性大小順序分別為pTGFα、pETα與pEGF。結果證實本研究室所開發之pEGF、pTGFα及其嵌合體皆有促進細胞生長之活性,而且其證實C結構域為提高細胞生長活性之所需。
Epidermal growth factor (EGF) and transforming growth factorα(TGFα) which can bind to the EGF receptor and activate receptor to become a potent mitogen, may help to establish the gastrointestinal mucosal barrier in neonatal animal. There is difference in the processing of intracellular internalization after the formation of ligand-receptor complexes between EGF and TGFα. The aim of this study was to
construct a pETα chimera which exchanging the N domain and C domain of pEGF and pTGFαand then compare its physiological activities among pEGF, pTGFα and its chimera pETα. The experiment was cloned the functional domain of pEGF, pTGFα and its chimera pETα genes by RT-PCR and subsequently transformed into Pichia pastoris to express recombinant proteins. SDS-PAGE electrophoresis, western blot, ELISA and cell proliferation assay were applied to examine the characters of these recombinant proteins. When the recombinant proteins expression in Pichia pastoris was induced by methanol, the secretary recombinant proteins in the supernatants was isolated. After SDS-PAGE electrophoresis, the extra band appeared at 6, 5.7 and 5.7 kDa which corresponds to the standard growth factors and immunoreactive to anti-pEGF, anti-pTGFαand anti-pTGFαantibody. The yields of recombinant proteins were the highest levels at 60 hrs after methanol induction, the expression level of pEGF、pTGFα and pETα in the cultured supernatant was 320, 118 and 27 mg/L, respectively. Three recombinant proteins all had the mitogenic activities to enhance the proliferation of 3T3 cells, and the order of stimulating activity was pTGFα、pETαand pEGF. The results suggest that all of the pEGF, pTGFαand pETαcould promote cell proliferation, and the C domain of the growth factor may contribute to enhance cell
proliferation.
誌謝................................................................i
中文摘要............................................................ii
英文摘要...........................................................iii
目錄...............................................................iv
圖目錄...............................................................x
表目錄.............................................................xii
縮寫與全名對照表...................................................xiii
壹、前言.............................................................1
貳、文獻檢討..........................................................2
參、材料與方法.......................................................23
肆、結果............................................................44
伍、討論............................................................68
陸、結論與未來展望....................................................73
柒、參考文獻.........................................................74
洪玉祥,余秉樺,張永鍾,陳博彥,李德南。甲醇指數進料策略對於重組Pichia pastoris生產豬表皮細胞生長因子之影響。宜蘭大學生物資源學刊(已接受)(2006)。

袁宇,張倩,黃培勇,王翼飛,周慶瑞,甘人寶,李載平。hEGF和hTGF-αN結構域與C結構域的功能差異。生物化學與生物物理學報,519-22 (1999)。

蘇遠志。微生物系統。教育部顧問室[生物技術科技教育改進計畫]後基因體時代之生物技術,139-157 (2003)。

葉萍,李燕,谷淑燕。MTS 和MTT法檢測小鼠NK及特異性细胞毒性T细胞活性的比較。中華實驗和臨床病毒學誌,85-86 (1998)。

唐元家,余柏松。巴斯德畢赤酵母表達系統。國外醫藥抗生素分冊,246-51 (2002)。

Abdullah, N. A., Torres, B. A., Basu, M. & Johnson, H. M.
Differential effects of epidermal growth factor, transforming growth factor-alpha, and vaccinia virus growth factor in the positive regulation of IFN-gamma production. J. Immunol. 143, 113-7 (1989).

Abud, H. E., Watson, N. & Heath, J. K. Growth of intestinal epithelium in organ culture is dependent on EGF signalling. Exp. Cell. Res. 303, 252-62 (2005).

Ainscough, S. L., Barnard, Z., Upton, Z. & Harkin, D. G. Vitronectin supports migratory responses of corneal epithelial cells to substrate bound IGF-I and HGF, and facilitates serum-free cultivation. Exp. Eye. Res. 83, 1505-14 (2006).

Alroy, I. & Yarden, Y. The ErbB signaling network in embryogenesis and oncogenesis: signal diversification through combinatorial ligand-receptor interactions. FEBS Lett. 410, 83-6 (1997).

Ancha, H. R., Ancha, H. B., Tedesco, D. S., Ward, A. R. & Harty, R. F. Inhibition of epidermal growth factor receptor activation enhances in vivo histamine -stimulated gastric acid secretion in the rat. Dig. Dis. Sci. 51, 274-81 (2006).

Arima, T., Tada, S., Makita, Y., Kurono, M. & Umeda, M. Effect of Clostridium perfringens-derived wound healing substance as compared with epidermal growth factor on the growth and morphological transformation of BALB/3T3 A31-1-1 cells. Mutat. Res. 341, 217-24 (1995).

Bamba, T., Tsujikawa, T. & Hosoda, S. Effect of epidermal growth factor by different routes of administration on the small intestinal mucosa of rats fed elemental diet. Gastroenterol. Jpn. 28, 511-7 (1993).

Bastaki, S. M., Chandranath, S. I. & Singh, J. Comparison of the antisecretory and antiulcer activity of epidermal growth factor, urogastrone and transforming growth factor alpha and its derivative in rodents in vivo. Mol. Cell. Biochem. 236, 83-94 (2002).

Berg, K., Zhai, L., Chen, M., Kharazmi, A. & Owen, T. C. The use of a water -soluble formazan complex to quantitate the cell number and mitochondrial function of Leishmania major promastigotes. Parasitol. Res. 80, 235-9 (1994).

Bramanti, V., Campisi, A., Tomassoni, D., Costa, A., Fisichella, A., Mazzone, V., Denaro, L., Avitabile, M., Amenta, F. & Avola, R. Astroglial-conditioned media and growth factors modulate proliferation and differentiation of astrocytes in primary culture. Neurochem. Res. 32, 49-56 (2007).

Buret, A., Olson, M. E., Gall, D. G. & Hardin, J. A. Effects of orally administered epidermal growth factor on enteropathogenic Escherichia coli infection in rabbits. Infect. Immun. 66, 4917-23 (1998).

Calnan, D. P., Fagbemi, A., Berlanga-Acosta, J., Marchbank, T., Sizer, T., Lakhoo, K., Edward, A. D. & Playford, R. J. Potency and stability of C terminal truncated human epidermal growth factor. Gut 47, 622-7 (2000).

Campion, S. R., Geck, M. K. & Niyogi, S. K. Cumulative effect of double-site mutations of human epidermal growth factor on receptor binding. J. Biol. Chem. 268, 1742-8 (1993).

Cereghino, J. L. & Cregg, J. M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24, 45-66 (2000).

Chailler, P. & Briere, N. Mitogenic effects of EGF/TGF alpha and immunolocalization of cognate receptors in human fetal kidneys. Biofactors 7, 323-35 (1998).

Ciana, P., Ghisletti, S., Mussi, P., Eberini, I., Vegoto, E. & Maggi, A. Estrogen receptor alpha, a molecular switch converting transforming growth factor-alpha-mediated proliferation into differentiation in neuroblastoma cells. J. Biol. Chem. 278, 31737-44 (2003).

Clare, J., Scorer, C., Buckholz, R. & Romanos, M. Expression of EGF and HIV envelope glycoprotein. Methods Mol. Biol. 103, 209-25 (1998).

Clare, J. J., Romanos, M. A., Rayment, F. B., Rowedder, J. E., Smith, M. A., Payne, M. M., Sreekrishna, K. & Henwood, C. A. Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies. Gene 105, 205-12 (1991).

Clare, J. J., Rayment, F. B., Ballantine, S. P., Sreekrishna, K. & Romanos, M. A. High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Biotechnology (N. Y.) 9, 455-60 (1991).

Cohen, S. The stimulation of epidermal proliferation by a specific protein (EGF). Dev. Biol. 12, 394-407 (1965).

Conde, R., Cueva, R., Pablo, G., Polaina, J. & Larriba, G. A search for hyperglycosylation signals in yeast glycoproteins. J. Biol. Chem. 279, 43789-98 (2004).

Cory, A. H., Owen, T. C., Barltrop, J. A. & Cory, J. G. Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cancer Commun. 3, 207-12 (1991).

Cos, O., Ramon, R., Montesinos, J. L. & Valero, F. A simple model-based control for Pichia pastoris allows a more efficient heterologous protein production bioprocess. Biotechnol. Bioeng. 95, 145-54 (2006).

Cregg, J. M., J. L. Cereghino, J. Shi & Higgins., D. R. Recombinant protein expression in Pichia pastoris. Mol. Biotechnol. 16, 23-52 (2000).

Daly, R. & Hearn, M. T. Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. J. Mol. Recognit. 18, 119-138 (2005).

Davies, P. F. Cell-cell contact and growth regulation of pinocytosis in 3T3 cells. J. Supramol. Struct. 13, 211-7 (1980).

Dignass, A. U. & Sturm, A. Peptide growth factors in the intestine. Eur. J. Gastroenterol. Hepatol. 13, 763-70 (2001).

Ellis, S. B., Brust, P. F., Koutz, A. F., Waters, A. F., Harpold, M. M. & Gingeras, T. R. Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris. Mol. Cell. Biol. 5, 1111-21 (1985).

Fahnestock, S. R. & Bedzyk, L. A. Production of synthetic spider dragline silk protein in Pichia pastoris. Appl. Microbiol.
Biotechnol. 47, 33-9 (1997).

Garcia, R., Franklin, R. A. & McCubrey, J. A. Cell death of MCF-7 human breast cancer cells induced by EGFR activation in the absence of other growth factors. Cell Cycle 5, 1840-6 (2006).

Gembitsky, D. S., Bozso, Z., O' Flaharty, M., Otvos, F., Murphy, R. F. & Lovas, S. A specific binding site for a fragment of the B-loop of epidermal growth factor and related peptides. Peptides 23, 97-102 (2002).

Gieni, R. S., Li, Y. & HayGlass, K. T. Comparison of [3H]thymidine incorporation with MTT- and MTS-based bioassays for human and murine IL-2 and IL-4 analysis. Tetrazolium assays provide markedly enhanced sensitivity. J. Immunol. Methods 187, 85-93 (1995).

Groenen, L. C., Nice, E. C. & Burgess, A. W. Structure-function relationships for the EGF/TGF-alpha family of mitogens. Growth Factors 11, 235-57 (1994).

Gustafsson, C., Govindarajan, S. & Minshull, J. Codon bias and heterologous protein expression. Trends Biotechnol. 22, 346-53 (2004).
Hardin, J. A., Chung, B., O'Loughlin E, V. & Gall, D. G. The effect of epidermal growth factor on brush border surface area and function in the distal remnant following resection in the rabbit. Gut 44, 26-32 (1999).

Hedemann, M. S., Hojsgaard, S. & Jensen, B. B. Small intestinal morphology and activity of intestinal peptidases in piglets around weaning. J. Anim. Physiol. Anim. Nutr. (Berl) 87, 32-41 (2003).

Hong, F., Meinander, N. Q. & Jonsson, L. J. Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris. Biotechnol. Bioeng. 79, 438-49 (2002).

Hsieh, P. & Robbins, P. W. Regulation of asparagine-linked oligosaccharide processing. Oligosaccharide processing in Aedes albopictus mosquito cells. J. Biol. Chem. 259, 2375-82 (1984).

Imanishi, J., Kamiyama, K., Iguchi, I., Kita, M., Sotozono, C. & Kinoshita, S. Growth factors: importance in wound healing and maintenance of transparency of the cornea. Prog. Retin. Eye Res. 19, 113-29 (2000).

Jaeger, L. A., Lamar, C. H., Cline, T. R. & Cardona, C. J. Effect of orally administered epidermal growth factor on the jejunal mucosa of weaned pigs. Am. J. Vet. Res. 51, 471-4 (1990).

Jahic, M., Gustavsson, M., Jansen, A. K., Martinelle, M. & Enfors, S. O. Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes. J. Biotechnol. 102, 45-53 (2003).

James, P. S., Smith, M. W., Tivey, D. R. & Wilson, T. J. Epidermal growth factor selectively increases maltase and sucrase activities in neonatal piglet intestine. J. Physiol. 393, 583-94 (1987).

Kim, S. H. & Akaike, T. Epidermal growth factor signaling for matrix-dependent cell proliferation and differentiation in primary cultured hepatocytes. Tissue Eng. 13, 601-9 (2007).

Kramer, R. H., Lenferink, A. E., van Bueren-Koornneef, I. L., van der Meer, A., van de Poll, M. L. & van Zoelen, E. J. Identification of the high affinity binding site of transforming growth factor-alpha (TGF-alpha) for the chicken epidermal growth factor (EGF) receptor using EGF/TGF-alpha chimeras. J. Biol. Chem. 269, 8708-11 (1994).

Kumar, V., Bustin, S. A. & McKay, I. A. Transforming growth factor alpha. Cell Biol. Int. 19, 373-88 (1995).

Lee, D. N., Kuo, T. Y., Chen, M. C., Tang, T. Y., Liu, F. H. & Weng, C. F. Expression of porcine epidermal growth factor in Pichia pastoris and its biology activity in early-weaned piglets. Life Sci. 78, 649-54 (2006).

Lenferink, A. E., Kramer, R. H., van Vugt, M. J., Konigswieser, M., Di Fiore, P. P., van Zoelen, E. J. & van de Poll, M. L. Superagonistic behaviour of epidermal growth factor/transforming growth factor-alpha chimaeras: correlation with receptor routing after ligand-induced internalization. Biochem. J. 327, 859-65 (1997).

Lenferink, A. E., van Zoelen, E. J., van Vugt, M. J., Grothe, S., van Rotterdam, W., van De Poll, M. L. & O'Connor-McCourt, M. D. Superagonistic activation of ErbB-1 by EGF-related growth factors with enhanced association and dissociation rate constants. J. Biol. Chem. 275, 26748-53 (2000).

Lenferink, A. E., De Roos, A. D., Van Vugt, M. J., Van de Poll, M. L. & Van Zoelen, E. J. The linear C-terminal regions of epidermal growth factor (EGF) and transforming growth factor-alpha bind to different epitopes on the human EGF receptor. Biochem. J. 336 (Pt 1), 147-51 (1998).

Li, Z., Xiong, F., Lin, Q., d'Anjou, M., Daugulis, A. J., Yang, D. S. & Hew, C. L. Low-temperature increases the yield of biologically active herring antifreeze protein in Pichia pastoris. Protein Expr. Purif. 21, 438-45 (2001).

Lopez, M., Koehler, A. & Nowak, G. High-level secretion of dipetarudin, a chimeric thrombin inhibitor, by Pichia pastoris. Protein Expr. Purif. 52, 8-13 (2007).

Lu, H. S., Chai, J. J., Li, M., Huang, B. R., He, C. H. & Bi, R. C. Crystal structure of human epidermal growth factor and its dimerization. J. Biol. Chem. 276, 34913-7 (2001).

Macauley-Patrick S., Fazenda, M. L., McNeil, B. & Harvey, L. M. Heterologous protein production using the Pichia pastoris expression system. Yeast 22, 249-270 (2005).

Marchbank, T., Boulton, R., Hansen, H. & Playford, R. J. Human transforming growth factor alpha (TGF-alpha) is digested to a smaller (1-43), less biologically active, form in acidic gastric juice. Gut 51, 787-92 (2002).

McGrew, J. T., Leiske, D., Dell, B., Klinke, R., Krasts, D., Wee, S. F., Abbott, N., Armitage, R. & Harrington, K. Expression of trimeric CD40 ligand in Pichia pastoris: use of a rapid method to detect high-level expressing transformants. Gene 187, 193-200 (1997).

Mehta, D. I., Horvath, K., Chanasongcram, S., Hill, I. D. & Panigrahi, P. Epidermal growth factor up-regulates sodium-glucose cotransport in enterocyte models in the presence of cholera toxin. J. Parenter. Enteral. Nutr. 21, 185-91 (1997).

Milani, S. & Calabro, A. Role of growth factors and their receptors in gastric ulcer healing. Microsc. Res. Tech. 53, 360-71 (2001).

Montaner, B., Asbert, M. & Perez-Tomas, R. Immunolocalization of transforming growth factor-alpha and epidermal growth factor receptor in the rat gastroduodenal area. Dig. Dis. Sci. 44, 1408-16 (1999).

Moon, H., Kim, S. W., Lee, J., Rhee, S. K., Choi, E. S., Kang, H. A., Kim, I. H. & Hong, S. I. Independent exponential feeding of glycerol and methanol for fed-batch culture of recombinant Hansenula polymorpha DL-1. Appl. Biochem. Biotechnol. 111, 65-80 (2003).

Murasugi, A. & Tohma-Aiba, Y. Production of native recombinant human midkine in the yeast, Pichia pastoris. Protein Expr. Purif. 27, 244-52 (2003).

Murphy, M. S. Growth factors and the gastrointestinal tract. Nutrition 14, 771-4 (1998).

Nestor, J. J., Jr., Newman, S. R., DeLustro, B., Todaro, G. J. & Schreiber, A. B. A synthetic fragment of rat transforming growth factor alpha with receptor binding and antigenic properties. Biochem. Biophys. Res. Commun. 129, 226-32 (1985).

Nice, E. C., Domagala, T., Fabri, L., Nerriea, M., Walker, F., Jorissen, R. N., Burgess, A. W., Cui, D. F. and Zhang, Y. S. Rapid microscale enzymic semisynthesis of epidermal growth factor (EGF) analogues. Growth Factors 20, 71-80 (2002).

Nylander-Koski, O., Mustonen, H., Puolakkainen, P., Kiviluoto, T. & Kivilaakso, E. Epidermal growth factor enhances intracellular pH regulation via calcium signaling in acid-exposed primary cultured rabbit gastric epithelial cells. Dig. Dis. Sci. 51, 1322-30 (2006).

Ouyang, J. Y., L., Long, Q. X., Wang, X. Z., Xing, K. & Wei, P. H. High level secretion expression of porcine somatoropin gene in Pichia pastoris and N-glycosylation analysis of products. Sheng Wu Gong Cheng Xue Bao 17, 520-5 (2001).

Pabbruwe, M. B., Stewart, K. & Chaudhuri, J. B. A comparison of colorimetric and DNA quantification assays for the assessment of meniscal fibrochondrocyte proliferation in microcarrier culture. Biotechnol. Lett. 27, 1451-5 (2005).

Pascall, J. C., Jones, D. S., Doel, S. M., Clements, J. M., Hunter, M., Fallon, T., Edwards, M. & Brown, K. D. Cloning and characterization of a gene encoding pig epidermal growth factor. J. Mol. Endocrinol. 6, 63-70 (1991).

Patel, M. I., Tuckerman, R. & Dong, Q. A pitfall of the 3-(4,5-dimethylthiazol-2-yl) -5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2 H-tetrazolium (MTS) assay due to evaporation in wells on the edge of a 96 well plate. Biotechnol. Lett. 27, 805-8 (2005).
Peng, Y. C. & Acheson, N. H. Production of active polyomavirus large T antigen in yeast Pichia pastoris. Virus Res. 49, 41-7 (1997).

Playford, R. J., Boulton, R., Ghatei, M. A., Bloom, S. R., Wright, N. A. & Goodlad, R. A. Comparison of the effects of transforming growth factor alpha and epidermal growth factor on gastrointestinal proliferation and hormone release. Digestion 57, 362-7 (1996).
Romanos, M. Advances in the use of Pichia pastoris for high-level gene expression. Current Opinion Biotech. 6, 527-33 (1995).

Scheiman, J. M., Meise, K. S., Greenson, J. K. & Coffey, R. J. Transforming growth factor-alpha (TGF-alpha) levels in human proximal gastrointestinal epithelium. Effect of mucosal injury and acid inhibition. Dig. Dis. Sci. 42, 333-41 (1997).

Scorer, C. A., Buckholz, R. G., Clare, J. J. & Romanos, M. A. The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene 136, 111-9 (1993).

Scorer, C. A., Clare, J. J., McCombie, W. R., Romanos, M. A. & Sreekrishna, K. Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. Biotechnology (N. Y.) 12, 181-4 (1994).

Sharp, P. M., Tuohy, T. M. & Mosurski, K. R. Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes. Nucleic Acids Res. 14, 5125-43 (1986).

Shen, W. H. & Xu, R. J. Stability of epidermal growth factor in the gastrointestinal lumen of suckling and weaned pigs. Life Sci. 59, 197-208 (1996).

Shioya, S. Optimization and control in fed-batch bioreactors. Adv. Biochem. Eng. Biotechnol. 46, 111-142 (1992).

Smith, G. E., Summers, M. D. & Fraser, M. J. Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol. Cell. Biol. 3, 2156-65 (1983).

Solari, V., Shima, H. & Puri, P. Increased expression of EGFR and TGF-alpha in segmental renal dysplasia in duplex kidney. Pediatr. Surg. Int. 20, 243-7 (2004).

Steinkamp, M. & Reinshagen, M. Transforming growth factor-alpha in the regulation of enterocytic proliferation: part of a puzzle. Eur. J. Gastroenterol. Hepatol. 13, 1285-7 (2001).
Tamariz, E., Hernandez-Quintero, M., Sanchez-Guzman, E., Arguello, C. & Castro-Munozledo, F. RCE1 corneal epithelial cell line: its variability on phenotype expression and differential response to growth factors. Arch. Med. Res. 38, 176-84 (2007).

Thiry, M. & Cingolani, D. Optimizing scale-up fermentation processes. Trends Biotechnol. 20, 103-5 (2002).

Trinh, L. B., Phue, J. N. & Shiloach, J. Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris. Biotechnol. Bioeng. 82, 438-44 (2003).

Turner, J. R., Liu, L., Fligiel, S. E., Jaszewski, R. & Majumdar, A. P. Aging alters gastric mucosal responses to epidermal growth factor and transforming growth factor-alpha. Am. J. Physiol. Gastrointest. Liver Physiol. 278, G805-10 (2000).

Uribe, J. M. & Barrett, K. E. Nonmitogenic actions of growth factors: an integrated view of their role in intestinal physiology and pathophysiology. Gastroenterology 112, 255-68 (1997).

Van de Poll, M. L., Lenferink, A. E., van Vugt, M. J., Jacobs, J. J., Janssen, J. W., Joldersma, M. & van Zoelen, E. J. A single amino acid exchange, Arg-45 to Ala, generates an epidermal growth factor (EGF) mutant with high affinity for the chicken EGF receptor. J. Biol. Chem. 270, 22337-43 (1995).

Van der Klei, I. J., Bystrykh, L. V. & Harder, W. Alcohol oxidase from Hansenula polymorpha CBS 4732. Methods Enzymol. 188, 420-7 (1990).

Van Zoelen, E. J., Stortelers, C., Lenferink, A. E. & Van de Poll, M. L. The EGF domain: requirements for binding to receptors of the ErbB family. Vitam. Horm. 59, 99-131 (2000).

Vaughan, T. J., James, P. S., Pascall, J. C. & Brown, K. D. Molecular cloning and tissue distribution of pig transforming growth factor alpha. Biochem. J. 296 (Pt 3), 837-42 (1993).

Vaughan, T. J., Pascall, J. C., James, P. S. & Brown, K. D. Expression of epidermal growth factor and its mRNA in pig kidney, pancreas and other tissues. Biochem. J. 279 (Pt 1), 315-8 (1991).

Vorauer, K., Steindl, F., Jungbauer, A., Hahn, R. & Katinger, H. Cytokine activity assay by means of proliferation measured in plane convex microtiter wells. J. Biochem. Biophys. Methods 32, 85-96 (1996).

Wagner, C. L., Forsythe, D. W. & Wagner, M. T. The effect of recombinant TGFalpha, human milk, and human milk macrophage media on gut epithelial proliferation is decreased in the presence of a neutralizing TGFalpha antibody. Biol. Neonate 74, 363-71 (1998).

Wang, L., Lucey, M. R., Fras, A. M., Wilson, E. J. & Del Valle, J. Epidermal growth factor and transforming growth factor-alpha directly inhibit parietal cell function through a similar mechanism. J. Pharmacol. Exp. Ther. 265, 308-13 (1993).

Wang, L., Wilson, E. J., Osburn, J. & DelValle, J. Epidermal growth factor inhibits carbachol-stimulated canine parietal cell function via protein kinase C. Gastroenterology 110, 469-77 (1996).

Werneburg, N. W., Yoon, J. H., Higuchi, H. & Gores, G. J. Bile acids activate EGF receptor via a TGF-alpha-dependent mechanism in human cholangiocyte cell lines. Am. J. Physiol. Gastrointest. Liver Physiol. 285, G31-6 (2003).

Wingens, M., Walma, T., van Ingen, H., Stortelers, C., van Leeuwen, J. E., van Zoelen, E. J. & Vuister, G. W. Structural analysis of an epidermal growth factor/transforming growth factor-alpha chimera with unique ErbB binding specificity. J. Biol. Chem. 278, 39114-23 (2003).

Wojciechowski, K., Trzeciak, L., Konturek, S. J. & Ostrowski, J. Inhibition of acid secretory response and induction of ornithine decarboxylase and its mRNA by TGF alpha and EGF in isolated rat gastric glands. Regul. Pept. 56, 1-8 (1995).
Wolfgang, C. L., Lin, C., Meng, Q., Karinch, A. M., Vary, T. C. & Pan, M. Epidermal growth factor activation of intestinal glutamine transport is mediated by mitogen-activated protein kinases. J. Gastrointest. Surg. 7, 149-56 (2003).

Yanai, R., Yamada, N., Inui, M. & Nishida, T. Correlation of proliferative and anti-apoptotic effects of HGF, insulin, IGF-1, IGF-2, and EGF in SV40-transformed human corneal epithelial cells. Exp. Eye Res. 83, 76-83 (2006).

Zhang, W., Hywood Potter, K. J., Plantz, B. A., Schlegel, V. L., Smith, L. A. & Meagher, M. M. Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement. J. Ind. Microbiol. Biotechnol. 30, 210-5 (2003).

Zhu, A., Monahan, C., Wang, Z. K. & Goldstein, J. Expression, purification, and characterization of recombinant alpha-N-acetylgalactosaminidase produced in the yeast Pichia pastoris. Protein Expr. Purif. 8, 456-62 (1996).
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關論文
 
1. 探討幹細胞生長因子合併其他生長因子對毛髮生長與黑色素生成之效用
2. 表皮生長因子、轉型生長因子-α_和表皮生長因子受器於唾腺黏液表皮樣癌和腺樣囊狀癌之表現
3. 由神經生長因子誘導之細胞內訊號路徑活化的化學基因體學分析
4. 探討胸腺基質淋巴生成素於第一型乙型轉型生長因子誘導腎與肺纖維母細胞纖維化的訊息路徑
5. 應用胞質內精子顯微注射膜受損精子產製基因轉殖豬胚之可行性
6. 研發猴頭菌發酵米漿及其抗氧化活性和免疫調節之研究
7. 建立即時定量PCR與螢光原位雜交法評估不同pH稀釋保存液對X、Y精子比率之影響
8. 體外生產豬胚之性別鑑定
9. 飼料添加丁酸鈉及羥丁胺酸對離乳仔豬生長性能、腸道發育及免疫反應之影響
10. 慢性肝病變患者血清中類胰島素生長因子-1、類胰島素生長因子結合蛋白-3與生長激素之檢測及其可能臨床應用研究
11. 第二型骨頭成型蛋白透過促進第一型乙型轉型生長因子接受器的代謝及抑制合成,拮抗乙型轉型生長因子誘導的腎細胞纖維化
12. 苯芘對纖維母細胞生長因子及血管內皮細胞生長因子所誘導之血管新生的抑制作用
13. 肝細胞生長因子基因重組蛋白之產製及其單源抗體製備
14. 羊乳生長因子之分離與鑑定
15. 表皮生長因子受器在高糖及第ㄧ型乙型生長因子誘導NRK-49F細胞生長與膠原蛋白產生之角色