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研究生:林月惠
研究生(外文):Yueh-Hui Lin
論文名稱:以表現pucF43-GFP果蠅來篩選和傷口癒合相關的基因
論文名稱(外文):Screening of genes related to wound healing by pucF43-GFP- expressing fly
指導教授:徐瑞洲
指導教授(外文):Jui- Chou Hsu
學位類別:碩士
校院名稱:國立清華大學
系所名稱:分子醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:48
中文關鍵詞:傷口癒合果蠅
外文關鍵詞:puckeredwound healinghepechinoidGFPfly
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傷口癒合的能力對於生物體的生長很重要,目前已知JNK訊息傳導路徑會在傷口癒合中被活化,puckered (puc)是JNK訊息傳導路徑下游基因,會表現在傷口周圍。之前研究建立了包含有puc基因上游1.6 kb DNA片段的轉殖基因果蠅pucF43-pGP,發現此果蠅傷口附近的細胞質有pucF43-GFP的表現。經過transcription Element Search System (TESS)的預測,我們知道這段1.6 kb的序列中包含有AP-1轉錄因子結合位置,所以我們把puc基因上游0.7 kb (pucF3)和0.7 kb ~ 1.6 kb (pucF4)的DNA片段分別插入細胞核表現GFP的載體中,建立出兩種轉殖基因果蠅pucF3-pHS和pucF4-pHS,發現這兩種果蠅的GFP表現亮度、分布、時間和pucF43-pGP果蠅沒有明顯差異。我們利用pucF43-pGP果蠅來篩選哪些基因和傷口癒合相關。發現hep1會增加GFP的表現,並減緩傷口癒合速度。ed突變基因沒有明顯或是一致地改變GFP表現,所以我們推測ed不會影響傷口癒合中JNK訊息傳導。
The ability of wound healing for organisms is important. The downstream of JNK signaling pathway, puckered, was activated in wound healing process and expressed around the wound. We make a transgenic fly pucF43-pGP including the upstream 1.6 kb non-coding DNA sequences of puc and expressing pucF43-GFP in cytoplasm. This DNA fragment showed many AP-1 transcriptional factor binding sites by TESS. We inserted the upstream 0.7 kb (pucF3) and 0.7-1.6 kb (pucF4) non-coding DNA sequences of puc into pH-Stinger vector which expressing nuclear GFP to generate pucF3-pHS and pucF4-pHS transgenic flies. These two transgenic flies had no difference in GFP expressing pattern, GFP intensity and the speed of wound healing with pucF43-pGP fly significantly. We took pucF43-pGP transgenic fly to screen genes related to wound healing. hep1 increased the GFP intensity, and slowed down the speed of wound healing. ed mutant didn’t affected GFP expressing pattern or GFP intensity, so that we can not prove that ed related to wound healing.
中文摘要……………………………………………..1
英文摘要……………………………………………..2
研究背景……………………………………………..3
材料與方法…………………………………………..7
實驗結果……………………………………………..10
討論…………………………………………………..15
參考文獻……………………………………………..19
圖表附錄……………………………………………..22
參考資料

王天成 (2006) 果蠅傷口癒合過程中puckered表現之研究 國立清華大學 分子醫學研究所碩士論文
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