臺灣博碩士論文加值系統

摘要
本研究係利用化學共沉法，合成出具有超順磁特性的奈米磁性粒子。因被介面活性劑包覆著，使奈米磁性粒子可在水溶液中均勻分佈。並進一步將還原型孔雀石綠(LMG)之抗體與奈米磁性粒子結合，形成一具有生物探針(bioprobe)之磁性生物標記(magnetic bio-marker)。最後，再利用本實驗室所研發之混頻交流磁導率(mixing frequency ac magnetic susceptibility)免疫檢測系統，探討檢測還原型孔雀石綠(LMG)之特性。

目錄
第一章 還原型孔雀石綠介紹...........................................................3
第二章 混頻交流磁導率(mixed-frequency ac magnetic
susceptibility)免疫檢測法...................................................7
第三章 混頻交流磁導率(mixed-frequency ac magnetic
susceptibility)免疫量測法檢測還原型孔雀石綠(LMG)
3.1檢測試劑製作
3.1.1 磁性奈米粒子製程方法與特性量測..................9
3.1.2 奈米磁性粒子與抗體鍵結之方法....................14
3.2 檢測流程及結果………………………………….…17
第四章 結論......................................................................................21
參考文獻.............................................................................................22
誌謝.....................................................................................................24

參考文獻
1. http://www.nchu.edu.tw/~material/nano/nanoinformation38.htm
2. Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein”, Appl. Phys. Lett.
3. http://www.miltenyibiotec.com/en/NN_27_MACS_Cell_Separation_Reagents.aspx
4. Ballanti (1985) Immunology III. p.198
5. http://juang.bst.ntu.edu.tw/
6. http://www.sdix.com/tsd/, http://www.accessexcellence.org/AB/GG/Antibody.html
7. P. M. Colman and W. R Tulip. From G..J.V.H. Nossal, 1993, Sci. Am 269(3):22.
8. http://www.uccs.edu/~rmelamed/MicroFall2002/Chapter%2017/Antibodies.html
9. http://www.bio.davidson.edu/COURSES/genomics/method/SDSPAGE/SDSPAGE.html
10. http://juang.bst.ntu.edu.tw/ECX/Pur3.htm#3.4.2
11. R. A. Goldsby, T. J. Kindt, B. A. Osborne, J. Kuby, FREEMAN,Immunology, New York
12. Determination of malachite green and leucomalachite green in carp muscle by liquid chromatography with visible and fluorescence detection. K. Mitrowaska, A. Posyniak, J Zmudzki, Journal of Chromatography A, 1089 187-192 (2005)
13. J. Bauer, J. Chromatography B: Biomedical Sciences and Applications, 722, 55(1999).
14. P.J. Fisher, M.J. Springett, A.B. Dietz, P.A. Bulur, and S. Vuk-Pavlovic, J. Immunological Methods, 262, 95(2002).
15. R. Kotitz, W. Weitschies, L. Trahms, W. Brewer, and W. Semmler, J. Magn. Magn. Mater., 194, 62(1999).
16. W.Q. Jiang, H.C. Yang, S.Y. Yang, H.E. Horng, J.C. Hung, Y.C. Chen, and Chin-Yih Hong, J. Magn. Magn. Mater., 283, 210 (2004).
17. H. E. Horng, S. Y. Yang, Y. W. Huang, W. Q. Jiang, C.-Y. Hong, and H.C. Yang, IEEE Trans. Appl. Supercond., 15, 668(2005).
18. H.E. Horng, Chin-Yih Hong, S.Y. Yang, H.C. Yang, S.H. Liao, C.M. Liu, and C.C. Wu, J. Korean Phys. Soc., 48, 999(2006)
19. H.C. Yang, S.Y. Yang, S.H. Liao, G.L. Fang, W.H. Huang, C.H. Liu, H.E. Horng, and Chin-Yih Hong, J. Appl. Phys., 99, 124701 (2006)
20. K. Enpuku, T. Minotani, T. Gima, Y. Kuroki, Y. Itoh, M. Yamashita, Y. Katakura, and S. Kuhara, Jpn. J. Appl. Phys., 38, L1102(1999)
21. Chin-Yih Hong, C.C. Wu, Y.C. Chiu, S.Y. Yang, H.E. Horng, and H.C. Yang, Appl. Phys. Lett., 88, 212512 (2006)
22. 物理雙月刊（廿八卷四期）2006年8月695
23. APPLIED PHYSICS LETTERS 90,074105(2007)
24. http://www.gabc-bio.com/pdt/mn/mn_types.php
25. 蘇志杰 , 「奈米粉體粒徑檢測方法簡介」，奈米機械技術專輯，機械工業雜誌，225期，第131-139頁。
26. T.Rheinländer , R. Kötitz, W. Weitschies,W. Semmler, Journal of Magnetism and Magnetic Materials 219 (2000) 219-228
27. http://global.horiba.com/analy_e/lb-550/measurement.htm
28. SeungKyun Lee, W. R. Myers, H. L. Grossman, H.-M. Cho, Y. R. Chemla, and John Clarke, Appl. Phy. Lett., 81, 3094(2002).
29. Q.A. Pankhurst, J. Connolly, S.K. Jones, and J. Dobson, J. Phys. D: Appl. Phys. 36, R167–R181 (2003).
30. S.Y. Yang, J.S. Sun, C.H. Liu, Chin-Yih Hong, H.C. Yang, and H.E. Horng, submitted to Adv. Mater. (2006).