臺灣博碩士論文加值系統

本實驗主要以一種新的低毒性高安定左旋維他命C複合物作可行性之應用分析，雖然文獻上提出使用硫代硫酸鈉可以有效的安定左旋維他命C，但是我們由實驗證明，硫代硫酸鈉與左旋維他命C複合後，卻會產生明顯的生物抑制性，故而加入無毒安全之天然蒙脫石，試圖使複合物毒性下降，使其具有高安定性低毒性之左旋維他命C複合物，以提升未來商業價值。由蒙脫石層間距變化發現到，左旋維他命C的添加將會影響到蒙脫石層間距的改變，而蒙脫石的遮蔽效應則會使左旋維他命C複合物成為安定且安全的材料，而在實驗中的複合物又以【MMT + 10 % LAA + 0.90 % Na2S2O3】 與 【MMT + 5 % LAA + 1.35 % Na2S2O3】毒性最低最為可行。

This study tended to construct new L-ascorbic acid (LAA) composites in low toxicity and high stability for feasible application. Although literature revealed that sodium thiosulphate could stabilize the activity of LAA, our finding showed that the significant toxicity was provoked by the introduction of sodium thiosulphate. Thus, a safe-and-non-toxic material montmorillonite (MMT) was thus used to attenuate this toxicity and to sustain the stability of composites with economic feasibility for practical use. As the proton released from LAA promoted the increases of interlayer spacing of clay layers in MMT, the formation of stable-and-non-toxic LAA composites was resulted as indicated from toxicity assessment (i.e., yeast model) and stability tests. The composites of 【MMT + 10 % LAA + 0.90 % Na2S2O3】 and 【MMT + 5 % LAA + 1.35 % Na2S2O3】were found to be feasible for further applications.

中文摘要 Ⅰ
英文摘要 Ⅱ
目錄 Ⅲ
圖索引 Ⅵ
表索引 ⅩⅢ
第一章 緒論 1
1-1前言 1
1-2 研究目的 4
第二章 理論基礎與文獻回顧 5
2-1 蒙脫石之晶體結構與應用 5
2-1-1蒙脫石的由來與製備 5
2-1-2 蒙脫石的理論構造及特性 7
2-1-3 蒙脫石之應用 13
2-2 左旋維他命C 15
2-2-1維他命C結構與特性 15
2-2-2維他命C的酸性反應 17
2-2-3維他命C的氧化 17
2-2-4維他命C的作用與功用 20
2-2-5維他命C於化妝品上的應用 23
2-3 硫代硫酸鈉特性與應用 24
2-4 毒性指標微生物―麵包酵母菌 25
2-4-1麵包酵母之特性與文獻回顧 25
2-4-2 微生物的生長模型 27
2-5 Probit model 之原理 28
2-6 合成成分毒性預估公式及運算法 32
第三章 實驗儀器與步驟 33
3-1 實驗儀器 33
3-2 實驗藥品 35
3-3 實驗方法及流程 36
3-3-1 硫代硫酸鈉對左旋維他命C安定度研究 36
3-3-2劑量-死亡率之毒理分析 37
3-3-3左旋維他命C/蒙脫石複合物之製備 39
3-3-4左旋維他命C/蒙脫石複合物之分析 41
3-3-4-1 XRD 分析 41
3-3-4-2 左旋維他命C含量於複合物之檢測 41
3-3-4-3 左旋維他命C/蒙脫石複合物毒性分析 42
第四章結果與討論 43
4-1 左旋維他命C安定度研究 43
4-1-1 左旋維他命C定量分析 43
4-1-2 L-ascorbic acid氧化度測定 46
4-1-3 添加不同Sodium thiosulfate莫耳比對L-ascorbic acid氧化度影響 47
4-2 劑量-死亡率毒性分析 53
4-3 左旋維他命C/蒙脫石複合物EDX分析結果 76
4-4左旋維他命C/蒙脫石複合物之XRD分析 78
4-5左旋維他命C含量於複合物之檢測 85
第五章 結論 91
參考文獻 93

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