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研究生:林君衡
研究生(外文):Chun-Heng Lin
論文名稱:利用抑制性扣減雜交法選出與茄子耐淹水有關基因
論文名稱(外文):Selection resist of flooding stress gene in eggplant by suppression subtractive hybridzation
指導教授:羅筱鳳
指導教授(外文):Hsiao-Feng Lo
學位類別:碩士
校院名稱:中國文化大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2006
畢業學年度:95
語文別:中文
論文頁數:69
中文關鍵詞:茄子淹水逆境抑制性扣減雜交即時聚合酶連鎖反應
外文關鍵詞:eggplantflooding stresssuppressing subtractive hybridizationreal time-PCR
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  • 下載下載:27
  • 收藏至我的研究室書目清單書目收藏:2
本研究利用抑制性扣減雜交法,篩選茄子根部在不同淹水時間具有差異性表現的基因,而了解茄子在淹水逆境下表現之功能性基因。以茄子EG117為試驗材料,無淹水處理做為控制組,淹水15 min、30 min、60 min、3 hr、6 hr、12 hr、24 hr、48 hr與72 hr做為實驗組,抽取各淹水時間其根部之RNA,做反轉錄,得到雙股cDNA,以substrative hybridization kit 之分析技術將淹水15 min~60 min及3 ~12 hr與無淹水之cDNA分別做扣減雜交,篩選出茄子根部淹水15~60 min、3~12 hr與未淹水具有差異性表現與茄子耐淹水有關之基因:新陳代謝相關的有9個;逆境相關的有8個;基因調控相關的有10個;發育相關的有1個;以及10個未知功能的基因。Real Time-PCR分析結果,則發現ABA 8'-hydroxylase mRNA、HSR201 protein mRNA、alcohol dehydrodenase mRNA、G/HBF-1 mRNA和MAP kinase-1 gene在茄子淹水3~12 hr有差異表現。
Genes in eggplant root after different flooding duration where selected by suppressing subtractive hybridization to analyze the functional genes and their displaying under flooding stress. The roots of EG117 were subjected to ten flooding durations, including 0, 15, 30 minutes and 1, 3, 6, 12, 24, 48 and 72 hours. Flooding-tolerance relative genes screened out included 9 genes correlated with metabolism, 8 genes related to stress, 10 genes related to gene intuction, 1 genes related to development, ten genes of unknown functions. Real time-PCR analysis showed the mRNA of ABA 8'-hydroxylase, HSR201 protein, alcohol dehydrodenase, G/HBF-1 mRNA and MAP kinase-1 displayed differently after 3~12 hr flooding.
目錄………………………………………………………….….……Ⅰ
表目錄……………………………………………………….….……Ⅱ
圖目錄………………………………………………………..………Ⅲ
縮寫表…………………………………………………….….……....Ⅴ
中文摘要……………………………………………….….....……...Ⅵ
英文摘要……………………………………………………..……...Ⅶ
壹、前言………………………………………………..…….........…1
貳、前人研究………………………………………..………..…...…2
參、材料與方法…………………………………….…………..........7
肆、結果……………………………………………………...….….15
伍、討論…………………………………………………….........…20
陸、結論…………………………………………….…..…......……27
柒、參考文獻……………………………………………..……..….56
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