臺灣博碩士論文加值系統

氧化壓力與發炎反應是影響致粥狀病變最重要的因素與促進者，由於影響血管內皮黏附分子的表現，在動脈粥狀硬化形成和慢性發炎中扮演重要角色。而前發炎細胞素-腫瘤壞死因子(tumor necrosis factor-alpha; TNF-α)則可以經由產生活性氧族群(reactive oxygen species; ROS)以引起動脈硬化過程中細胞黏附分子ICAM-1與 VCAM-1的產生。本研究主要探討葉綠素相關化合物包括：葉綠素a+b混合物、葉綠素a、葉綠素b、銅鈉葉綠素對TNF-α誘導人類主動脈內皮細胞(human aortic endothelial cells；HAEC)發炎反應的影響。結果顯示葉綠素a+b混合物及葉綠素a、葉綠素b和銅鈉葉綠素在10μM濃度以下對HAEC不具細胞毒性；各10μM葉綠素a+b混合物、葉綠素a、葉綠素b和銅鈉葉綠素顯著降低U937與HAEC的黏附活性，其抑制率分別為92.0±1%、49.5±2.2%、82.4±2.1% 與47.4±3.3%；並且葉綠素a+b混合物、葉綠素a、葉綠素b、銅鈉葉綠素與Aspirin顯著抑制黏附因子VCAM-1和ICAM-1a±1.3% 和30±2.1%、32.6±1.3%、51.3±3.2%、13.5±2.3%、28±3.3%。綜合研究結果證實葉綠素相關化合物可抑制前發炎因子TNF-α作用，顯著抑制黏附因子VCAM-1和ICAM-1的表現，尤其是Chl a與Chl b有助於減輕發炎反應。

Oxidative stress and inflammation are thought to be an important contributor and promoter of atherogenesis. While altered expression of cell adhesion molecules by the arterial endothelium plays a major role during atherogenesis and chronic inflammation. The pro-inflammatory cytokine TNF-α (tumor necrosis factor-alpha) could induce ICAM-1 (intercellular adhesion molecule-1) and VCAM-1 (vascular cell adhesion molecule-1) in atherogenesis through ROS activation. The aim of this study was to discuss the effect of chorophyll-related compounds (chlorophyll a+b, chlorophyll a, chlorophyll b and chorophyllin) on inflammatory response in TNF-α treated human aortic endothelial cells(HAEC). At 10μM chlorophyll a+b, chlorophyll a, chlorophyll b, and chorophyllin had no toxicity to HAEC. Chlorophyll a+b, chlorophyll a, chlorophyll b and chorophyllin inhibited the endothelial cell-leukocyte adhesion percentage up to 92.0±1%, 49.5±2.2%, 82.4±2.1% and 47.4±3.3%, respectively. At the same concentration of chlorophyll a+b, chlorophyll a, chlorophyll b, chorophyllin and Aspirin also significantly inhibited the expression of cell adhesion molecules, VCAM-1 and ICAM-1 by 46.6±3.2%, 50±2.2%, 51±2.5%, 30.2±2.1%, 44.1±1.3% and 30±2.1%, 32.6±1.3%, 51.3±3.2%, 13.5±2.3% , 28±3.3%, respectively. To conclusion, chorophyll-related compounds significantly inhibit TNF-αinduced the expression of cell adhesion molecules, VCAM-1 and ICAM-1. It may slow down inflammatory response, particularly in Chl a and Chl b treated groups.

目錄

中文摘要 ----------------------------------------------------- 1
英文摘要 ------------------------------------------------------3
第一章 緒論----------------------------------------------------4
第二章 文獻回顧
一、葉綠素及其衍生物之生物功能
(一)葉綠素及其衍生物之結構----------------------------------------6
(二)葉綠素來源及合成路徑------------------------------------------9
(三)葉綠素相關化合物各論-----------------------------------------11
二、動脈粥狀硬化之簡介
(一)動脈硬化的致病機轉-------------------------------------------13
(二)動脈硬化與腫瘤壞死因子的相關性---------------------------------20
(三)動脈硬化與細胞表面黏附分子之相關性------------------------------20
(四)血管內皮細胞之重要性------------------------------------------24
(五)動脈硬化與氧化壓力之相關性-------------------------------------24
第三章 研究目的與架構----------------------------------------------31
第四章 實驗材料與方法
一、無菌操作基本技術-------------------------------------------33
二、HAEC(human aortic endothelial cells)的培養------------------35
三、材料-------------------------------------------------------36
四、實驗方法----------------------------------------------------38
第五章 實驗結果---------------------------------------------------41
第六章 討論-------------------------------------------------------52
第七章 結論-------------------------------------------------------57
參考文獻----------------------------------------------------------58

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