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研究生:趙偉如
研究生(外文):CHAO WEI JU
論文名稱:苦茶油對內毒素血症大白鼠器官損傷的效應
論文名稱(外文):Effects of Camellia Seed Oil on Organ Injury in Endotoxemic Rats
指導教授:謝建正謝建正引用關係施明智施明智引用關係
指導教授(外文):Chien-Cheng Hsieh Ph.D.Ming-Chih Shih Ph.D.
學位類別:碩士
校院名稱:中國文化大學
系所名稱:生活應用科學研究所
學門:民生學門
學類:生活應用科學學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:77
中文關鍵詞:內毒素血症脂多醣苦茶油
外文關鍵詞:endotoxmialipopolysaccharidecamellia seed oil
相關次數:
  • 被引用被引用:2
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  • 下載下載:115
  • 收藏至我的研究室書目清單書目收藏:3
摘要
近年研究證實細菌內毒素脂多醣(lipopolysaccharide,LPS)刺激免疫細胞產生活性氧分子(reactive oxygen species,ROS)及細胞激素,ROS及細胞激素會造成敗血症個體多重器官衰竭。苦茶油是具有高清除活性氧能力的食用油,然而並無研究探討苦茶油對LPS誘發器官損傷的效果,因此本研究計畫探討之。我們使用LPS注射誘發內毒素血症動物模式進行研究,SD大白鼠(270-300克)接受腹腔注射LPS(5mg/kg BW),然後接受皮下補充苦茶油(8ml/kg BW),LPS注射後12小時,記錄存活率並測量血清生化值。我們也測量血漿、肝、腎檢體和丙二醛(malondialdehyde,MDA)。最後,我們也測抗氧化酵素活性,包括過氧化酶(catalase)、麩胱甘肽過氧化酶(glutathione peroxidase,GPx)和超氧岐化酶(superoxide dismutase,SOD),結果顯示苦茶油補充提高內毒素血症大白鼠存活率(100% v.s. 69.2%),苦茶油也能減輕內毒素血症大白鼠器官損傷現象。而玉米油補充(對照組)並不會降低死亡率及生化值。至於抗氧化酵素活性,LPS顯著降低GPx、血漿SOD活性,但對catalase活性無影響,而玉米油或苦茶油補充對於接受生理食鹽水或LPS注射大白鼠的三種抗氧化酵素活性均無影響。總之,苦茶油可降低內毒素血症大白鼠器官損傷及死亡率,但無法減少LPS誘發的脂質過氧化作用,苦茶油亦無法維持接受LPS注射大白鼠的抗氧化酵素活性,因此苦茶油減輕內毒素反應的詳細機制仍需進一步研究探討。
Abstract

Recent studies had demonstrated bacterial endotoxin lipopolysaccharide(LPS)stimulate reactive oxygen species(ROS)and cytokines productions of immune cells. ROS and cytokines multiple organ failure in septic individuals. Camlellia oil is one of edible oils with high ROS-scavenging ability. However, there is no study to investigate the effects of camellia seed oil on LPS-induced organ injuries and mortality. The aims of present study were to investigate this issue. In our studies, Sprague-Dawley(SD, 275-300g)rats received intraperitoncal LPS(5mg/kg BW)-injection, and then received camellia seed oil (8ml/kg BW)injection subcutaneously. At 12 hour-post LPS injection, survival rate, serum biochemistry of rats were determined. We also measured malondialdehyde(MDA)levels of plasma, liver and kidney samples. Finally, we measured the activities of antioxidative enzymes, including catalase(CAT), Glutathione peroxidase(GPx)and superoxide dismutase(SOD)of plasma, liver, and kidney samples. Our results had shown that camellia seed oil supplementation improve survival rate of endotoxemic rats(100% v.s. 69.2%). Camellia seed oil also attenuated organ injuries of LPS-treated rats. However, corn oil supplementation (control group)did not attenuate mortality and elevated blood chemistry levels in LPS-treated rats. In antioxidative enzymes activities, LPS significantly reduced the activities of glutathione peroxidase and plasma SOD, but not catalase. Corn oil or camellia seed oil supplementation had no effect on three antioxidative enzymes activities in saline or LPS-treated rats. In conclusion, camellia seed oil attenuated organ injuries and mortality rate in endotoxemic rats, but didn’t reduced LPS-induced peroxidation. Camellia seed oil also didn’t maintained the antioxidative enzymes activities in LPS-treated rats. Therefore, the mechanisms involving the protective effects of camellia seed oil need further investigations.
目錄

壹、前言(包括研究背景、動機、目的) 1
貳、文獻探討 3
一、苦茶油的介紹 3
(一)油茶的品種 3
(二)苦茶油成份分析 3
二、苦茶油生理功能的相關研究 5
(一)抗氧化能力 5
(二)抗菌的功能 6
(三)保護器官的功能 6
(四)預防冠心病 7
三、內毒素血症的致病機轉 9
參、材料與方法 15
一、實驗架構 15
二、實驗材料 17
(一)實驗動物 17
(二)實驗藥品 17
(三)實驗套組 18
(四)實驗儀器 19
三、實驗方法 20
(一)實驗組施打脂多醣及經皮下補充油脂的方式 20
(二)對照組施打生理食鹽水及經皮下補充油脂的方式 20
(三)血液樣品的收集 21
(四)器官樣品的收集 21
(五)測肝腎功能生化指標 21
(六)脂質過氧化物的測量 22
(七)評估抗氧化酵素活性 23
(八)統計分析 26
肆、結果與討論 27
一、結果 27
(一)存活率 27
(二)生化參數 28
(四)抗氧化酵素活性變化量 33
二、討論 40
(一)利用脂多醣誘發大白鼠內毒素血症與存活率評估 40
(二)皮下補充苦茶油對於內毒素血症大白鼠肝腎損傷的影響 41
(三)皮下補充苦茶油對內毒素血症大白鼠脂質過氧化的情形 42
(四)苦茶油對內毒素血症大白鼠體內的抗氧化酵素系統之作用 43
伍、結論 48
陸、參考文獻 66

圖目錄

圖一、大白鼠血清丙胺酸轉氨酶濃度…………………………………………..49
圖二、大白鼠血清總膽紅素濃度………………………………………………..50
圖三、大白鼠血清血液尿素濃度………………………………………………..51
圖四、大白鼠血清肌酸酐濃度…………………………………………………..52
圖五、大白鼠血漿丙二醛含量…………………………………………………..53
圖六、大白鼠肝臟組織丙二醛含量……………………………………………..54
圖七、大白鼠腎臟組織丙二醛含量…………………………………………..…55
圖八、大白鼠肝臟組織過氧化酶活性………………………………………..…56
圖九、大白鼠腎臟組織過氧化酶活性…………………………………………..57
圖十、大白鼠血漿超氧岐化酶活性………………………………………..……58
圖十一、大白鼠肝臟組織超氧岐化酶活性……………………………..………59
圖十二、大白鼠腎臟組織超氧岐化酶活性…………………………………..…60
圖十三、大白鼠血漿麩胱甘肽過氧化酶活性………………………………..…61
圖十四、大白鼠肝臟麩胱甘肽過氧化酶活性……………………..……………62
圖十五、大白鼠腎臟麩胱甘肽過氧化酶活性……………………………..……63
圖十六、大白鼠血清中尿酸的含量………………………………………..……64
王振瀾、尹華文、劉文玉(1994)茶油之穩定性探討及生育酚與固醇類成份之分析。林業試驗所研究報告季刊,9:73-86。

王振瀾、林玉含(1990)優良品種油茶之油脂成分提煉及性質分析。林業試驗所研究報告季刊,5:11-15。

王鼎耀(2006)兒茶素補充對敗血症大白鼠多重器官損傷之效應。中國文化大學生活應用科學研究所碩士論文。

古國隆(1996)苦茶油抗菌因素之研究。行政院國家科學委員會專題研究計畫成果報告。

古國隆(1998)苦茶油抗氧化物質之研究-I高溫焙炒生成之苦茶油抗氧化物質。行政院國家科學委員會專題研究計畫成果報告。

行政院衛生署統計室(2005),台灣地區統計結果摘要。

邱年永、張光雄(1987)原色台灣藥用植物圖鑑(一)。台北:南天書局,第98頁。

胡晋豪(2006)補充兒茶素對內毒素血症大白鼠多重器官損傷之效應。中國文化大學生物科技研究所碩士論文。

唐茂云、張文敏、胡曼玲、黃憶明、周光宇、章滿(1994)食用油脂對血脂、脂質過氧化、膜磷脂脂肪酸及血小板功能的影響。營養學報,16:239-246。

張兵、周韞珍(1995)茶油、豆油對大鼠體內活性氧及抗氧化酶活性影響的研究。營養學報,17:199-203。

賈玉巧、楊東偉、趙曉紅(2005)茶油遺傳毒性的評價。北京聯合大學學報,19:66-68。

陳宇輝、陳榮洲(2001)比較茶油與沙拉油抗實驗性胃潰瘍的作用。中西整合醫學雜誌,3:31-37。

陳梅芳、顧景范、孫明堂、楊惠彬、張樞泉、劉玉軍(1996)茶油延緩動脈粥樣硬化形成及其機理的探討。營養學報,18:13-19。

顏國欽(2004)苦茶油(Camellia oleifera Abel.)機能性成分及其應用之研究。行政院農業委員會專題研究計畫期末成果報告。

Alexander HR, Doherty GM, Buresh CM, Venzon DJ, Norton JA(1991)A recombinant human receptor antagonist to interleukin 1 improves survival after lethal endotoxemia in mice. J. Exp. Med., 173, 1029-1032.
Aktan F.(2004)iNOS-mediated nitric oxide production and its regulation. Life Sciences,75,639-653.

Akihisa T, Tokuda H, Ukiya M, Suzuki T, Enjo F, Koike K, Nikaido T, Nishino H.(2004)3-Epicabraleahydroxylactone and other triterpenoids from camellia oil and their inhibitory effects on Epstein-barr virus activation. Chem. Pharm. Bull.,52, 153-156.
Ana Márquez Martín , Rocío de la Puerta Vázquez , Angeles Fernández-Arche , Valentina Ruiz-Gutiérrez.(2006) Supressive effect of maslinic acid from pomace olive oil on oxidative stress and cytokine production in stimulated murine macrophages. Free radic. Res., 40, 295-302.
Balasubramantan KA, Manohar M, Mathan VI.(1988)An unidentified inhibitor of lipid peroxidation in intestinal mucosa. Biochim Biophy Acta., 962, 51-58.
Berry EM, Eisenberg S, Friedlander Y, Harats D, Kaufmann NA, Norman Y, Stein Y. (1992)Effects of diets rich in monounsaturated fatty acids on plasma lipoproteins-the Jerusalem Nutrition Study. II. Monounsaturated fatty acids vs carbonhydrates. Am J. Clin. Nutr. 56, 394-403.
Beutler B, Milsark IW, Cerami AC. (1985)Passive immunization against cachectin /tumor necrosis factor protects mice from lethal effect of endotoxin. Science, 229, 869-871.
Bone RC, Fisher CJ , Clemmer TP, Slotman GJ, Metz CA, Balk RA.(1989)Sepsis syndrome:a valid clinical entity. methylpredisolone severe sepsis study group. Crit Care Med, 17, 389-393.
Bonanome A, Pagnan A, Biffanti S, Opportuno A, Sorgato F, Dorella M, Maiorino M, Ursini F. (1992)Effect of dietary monounsaturated and polyunsaturated fatty acids on the susceptibility of plasma low density lipoproteins to oxidative modification. Arterioscler. Thromb. 12, 529-533.
Budiyanto A, Ahmed NU, Wu A, Bito T, Nikaido O, Osawa T, Ueda M, Ichihashi M.(2000)Protective effect of topically applied olive oil against photocarcinogenesis following UVB exposure of mice. Carcinogenesis, 21, 2085-2090.
Crespo E, Macias M, Pozo D, Escames G, Martin M, Vives F, Guerrero JM, Acuna-Castroviejo D.(1999)Melatonin inhibits expression of the inducible NO synthase II in liver and lung and prevents endotoxemia in lipopolysaccharide induced multiple organ dysfunction syndrome in rats. FASEB J , 13, 1537-1546.
Deshpande R, Khalili H, Pergolizzi RG, Michael SD, Chang MD.(1997)Estradiol down-regulates LPS-induced cytokine production and NFkB activation in murine macrophages. Am J Reprod Immunol., 38,46-54.
Dinarello CA, Cannon JG.(1993)Cytokine measurements in septic shock. Ann. Intern. Med, 119, 853-854.
Glatzle J, Beckert S, Kasparek MS, Mueller MH, Mayer P, Meile T, Konigsrainer A, Steurer W. (2007)Olive oil is more potent than fish oil to reduce septic pulmonary dysfunctions in rats. Langenbecks Arch Surg, 392, 323-329.
Helen FG, Michael JD, Nigel RW.(1996)Xanthine oxidase activity and free radical generation in patients with sepsis syndrome. Crit Care Med, 24,1649-1653.
Hsu DZ, Li YH, Chu PY, Chien SP, Chuang YC and Liu MY.(2006)Attenuation of endotoxin-induced oxidative stress and multiple organ injury by 3,4-methylenedioxyphenol in rats. Shock , 25, 300-305.
Hsu DZ, Liu MY.(2004)Effects of sesame oil on oxidative stress after the onset of sepsis in rats. Shock , 22, 582-585.
Hsu DZ, Lin MY.(2002)Sesame oil attenuates multiple organ failure and increases survival rate during endotoxemia in rats. Crit Care Med., 30, 859-862.
Hsu DZ, Lin MY.(2004)Sesame oil protects against lipopolysaccharide-stimulated oxidative stress in rats. Crit Care Med., 32, 227-231.
Hsu DZ, Su SB, Chien SP, Chiang PJ, Li YH, Lo YJ, Liu MY.(2005)Effect of sesame oil on oxidative-stress-associated renal injury in endotoxemic rats:involvement of nitric oxide and proinflammatory cytokines. Shock, 24, 276-280.
Hsu HY, Wen MH.(2002)Lipopolysaccharide-mediated reactive oxygen species and signal transduction in regulation of interleukin-1 gene expression. J. Biol. Chem., 277,22131-22139.
Iszard MB, Liu J, Klaassen CD.(1995)Effect of several metallothionein inducers on oxidative stress defense mechanisms in rats. Toxicology, 104, 25-33.
Katan MB, Zock PL, Mensink RP.(1995)Dietary oils, serum lipoproteins, and coronary heart disease. Am. J. Clin. Nutr. 61, 1368s-1373s.
Kelly A. M., Suresh E. V. K., Stewart J. W., Jens R. C., Apurba D., Unil A. D.(2005)Lipopolysaccharide Sequestrants: Structural Correlates of Activity and Toxicity in Novel Acylhomospermines. J. Med. Chem., 48, 2589 -2599.
Lee C. P., Yen G. C.(2006)Antioxidant activity and bioactive compounds of tea seed (Camellia oleifera Abel.)oil. J. Agric .Food Chem., 54, 779-784.
Llesuy S, Evelson P, Gonzalez-Flecha B, peralta J, Carreras MC, Poderoso JJ, Boveris A.(1994)Oxidative stress in muscle and liver of rats with septic syndrome. Free Radic Biol Med., 16, 445-451.

Liaw WJ, Tzao C, Wu JY, Chen SJ, Wang JH, Wu CC.(2003)Inhibition by terbutaline of nitric oxide and superoxide anion levels of endotoxin-induced organs injury in the anesthetized rat. Shock. 19, 281-288.

Liu ZZ, Cui S, Billiar TR, Dorko K, Halfter W, Geller DA, Michalopoulos G, Beger HG, Albina J, Nussler AK.(1996)Effects of hepatocellular mitogens on cytokine-induced nitric oxide synthesis in human hepatocytes. J Leukocyte Biol., 60, 382-388.
Manna C, Galletti P, Cucciolla V, Montedoro G, Zappia V.(1999)Olive oil hydroxytyrosol protects human erythrocytes against oxidative damages. J. Nutr. Biochem, 10, 159-165.

Mata P, Varela O, Alonso R, Lahoz C, Badimon L.(1997)Monounsaturated and polyunsaturated n-6 fatty acid-enriched diets modify LDL oxidation and decrease human coronary smooth muscle cell DNA synthesis. Arterioscler. Thromb. Vasc. Biol. 17, 2088-2095.

Marchant A, Bruyns C, Vandenabeele P, Ducarme M, Gerard C, Delvaux A, Groote D, Abramowicz D, Velu T, Goldman M.(1994)Interleukin-10 controls interferon-gamma and tumor Necrosis factor production during experimental endotoxemia. Eur. J. Immunol. 24, 1167-1171.

Marshall BJ. (1983) Unidentified curved bacilli in gastric epithelium in active chronic gastritis. Lancet, 1, 1273-1275.

Marshall BJ., Warren JR. (1984) Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet, 1, 1311-1315.

Mayer K, Gokorsch S, Fegbeutel C, Hattar K, Rosseau S, Walmrath D, Seeger W.(2003)Parenter nutrition with fish oil modulates cytokine response in patients with sepsis. Am J Respir Crit Care Med., 167, 1321-1328.
McKelvey TG, Willwarth ME, Granger DN.(1988)Mechanisms of conversion of xanthine dehydrogenase to xanthine oxidase in ischemic rat liver and kidney. Am J Physiol, 254, G753-G760.

Milane SL, Patricia P, Rachel NG, Alexandre TG, Hugo CC, Patricia TB, Vera LG. (2005)Mechanism of increased survival after lipopolysaccharide-induced endotoxic shock in mice consuming olive oil-enriched diet. Shock, 23, 173-178.

Miura D, Kida Y, Nojima H.(2007)Camellia oil and its distillate fractions effectively inhibit the spontaneous metastasis of mouse melanoma BL6 cells. FEBS Letters, 581, 2541-2548.

Moriya S, Yokoyama H, Fukuda M, Okamura Y, Kamegaya Y, Mizukami T, Ohgo H, Ishii H.(2000)Glutathione depletion enhances the formation of superoxide anion released into hepatic sinusoids after lipopolysaccharide challenge. Alcohol Clin Exp Res, 24, 59-63s.

Pfeffer K, Matsuyama T, Kundig TM, Wakeham A, Kishihara K, Shahinlan A, Wiegmann K, Ohashi PS, Kronke M, Mak TW. (1993)Mice defficient for the 55 Kd tumor necrosis factor receptor are resistant to endotoxic shock. Cell, 73, 457-467.

Philip CC.(2004)N-3 Fatty Acids, inflammation, and immunity- relevance to postsurgical and critically ill patients. Lipid, 39, 1147-1161.

Poggetti RS, Morre FA, Morre EE.(1992)Liver injury is a reversible neutrophil mediated event following gut ischemia. Arch Surg, 127, 175-179.

Poggetti RS, Morre FA, Morre EE.(1992)Simultaneous liver and lung injury following gut ischemia is mediated by xanthine oxidase. J Trauma. 32, 723-728.

Presanti EL, Nugent KM.(2004)Modulation of pulmonary clearance of bacteria by antioxidants. Infect. immun.,48, 57-61.

Reaven P, Parthasarathi S, Grasse BJ, Miller E, Almazan F, Mattson FH, Khoo JC, Steinberg D, Witztum JL.(1991)Feasibility of using an oleate-rich diet to reduce the susceptibility of low-density lipoprotein to oxidative medication in humans. Am. J. Clin. Nutr. 54, 701-706.

Schmeling DJ, Caty MG, Oldham KT.(1989)Evidence for neutrophil related acute lung injury after intestinal ischemia and reperfusion. Surgery, 106, 195-202.

Susana C, Ana MC.(2002)Fighting the stranger-antioxidant protection against endotoxin toxicity. Toxicology, 180, 45-63.

Salvemini D, Cuzzocrea S.(2002)Oxidative stress in septic shock and disseminated intravascular coagulation. Free Radic. Biol. Med., 33, 1173-1185.
Spolarics Z, Wu JX.(1997)Role of glutathione and catalase in H2O2 detoxification in LPS-activated hepatic endothelial and Kupffer cells. Am J Physiol Gastrointest Liver Physiol, 273, 1304-1311.

Takeyama N, Shoji Y, Ohashi K, Tanaka T.(1996)Role of reactive oxygen intermediates in lipopolysaccharide-mediated hepatic injury in the rat. J Surg Res, 60, 258-262.

Taylor DE, Piantadosi CA.(1995)Oxidative metabolism in sepsis and sepsis syndrome. J Crit Care, 10, 122-135.

Traccy KJ, Fong Y, Hesse DG, Manogue KR, Lee AT, Kuo GC, Lowry SF, Cerami AC. (1987)Anti-cachectin/TNF monoclonal antibodies prevent septic shock during lethal bacteraemia, Nature, 330, 662-664.

Trager K, DeBacker D, Radermacher P.(2003)Metabolic alterations in sepsis and vasoactive drug-related metabolic effects. Curr Opin Crit Care.9, 271-278.

Wang A, Seki T, Yuan D, Saso Y, Hosoya O, Chono S, Morimoto K.(2004)Effect of camellia oil on the permeation of flurbiprofen and diclofenac sodium through rat and pig skin. Biol. Pharm. Bull. 27, 1476-1479.

Wheeler DS, Catravas JD, Odoms K, Denenberg A, Malhotra V, Wong HR.(2004)Epigallocatechin-3-gallate, a green tea-derived polyphenol, inhibits IL-1β-dependent proinflammatory signal transduction in cultured respiratory epithelial cells. J. Nutr. 134,1039-1044.

Yasumoto SS, Katsuta M, Okuyama Y, Takahashi Y, Ide T.(2001)Effect of sesame seeds rich in sesamin and sesamolin on fatty acid oxidation in rat liver. J. Agric. Food Chem. 49, 2647-2651.

Yokozawa T, Rhyu DY, Chen CP.(2003)Protective effect of acanthopanax radix extract against endotoxemia induced by lipopolysaccharide. Phytother Res. 17, 353-357.

Zimmerman JJ.(1995)Defining the role of oxygen radicals in the pathogenesis of sepsis. Crit Care Med. 23, 616-617.
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