臺灣博碩士論文加值系統

近年來許多研究指出許多中草藥由於具有生理活性物質，在臨床治療上具有相當的成效。本研究的目的是探討利用何首烏單方及複方對於倉鼠之降血脂及體內抗氧化能力的功效。本研究是以檢測倉鼠生長情形、糞便中脂肪代謝、血液生化值分析、脂肪肝檢測、肝臟脂質含量分析、體內抗氧化能力等方法以評估何首烏單方及複方的生理效用。結果顯示倉鼠經10週何首烏藥劑的餵食，何首烏複方對於倉鼠隻日增重以及飼料利用率沒有影響，然而中藥單方組的生長速率則有降低的情形。且對血膽固醇及血脂質均有顯著的降低（p＜0.05）。而肝臟中膽固醇及三酸甘油酯含量皆有顯著的降低（p＜0.05），但對於肝臟中脂肪堆積所誘發的肝臟傷害則無顯著改善效果。何首烏單方與複方降血脂的機轉可能是降低肝臟釋放VLDL以及增加周圍組織清除LDL-C，以達到降血膽固醇濃度的效果，並且可以減少肝臟脂肪的屯積，唯對於脂肪肝的改善效果並不顯著。經過10週的餵食，何首烏單複方對倉鼠的體內抗氧化能力有顯著的增加，包括肝臟及腎臟的麩胱甘過氧化（GSH-Px）及超氧歧化（SOD）活性均有顯著增加的效果，且對於倉鼠組織及血清中的丙二醛含量皆有顯著降低。根據這些發現，可以推論何首烏複方比何首烏單方對血脂異常的病患更有降低血脂肪的效果。

It is reported that Chinese herbs, with many functional ingredients, can be used effectively in clinical therapy. The purpose of this in vivo study was to investigate the effect of Polygonum mulitiflorum Thunb. on blood lipid profile and antioxidative status in hamster. The single and complex formula of Polygonum mulitiflorum Thunb. were used in this study. After 10 weeks of feeding periods, growth rate, fecal content, plasma total cholesterol and triglyceride concentrations, liver function and antioxidative status were measured in these animals. The results of this study showed that feed intake and daily gain did not affected by the feeding of complex formula, however, growth rate was impaired in single formula groups. Both formulas significantly decreased plasma total cholesterol and triglyceride concentrations in hamster after 10 weeks feeding (p<0.05). However, liver injury due to fat accumulation did not affected by these formulas. The mechanism(s) of blood cholesterol lowering action by complex formula may be mediated by decreasing VLDL secretion from the liver, or increasing LDL clearance from peripheral circulation. After 10 weeks, the antioxidative capacity was increased significantly by feeding both formulas to these animals. In single and complex formula fed groups, the activity of glutathione peroxidase and superoxide dismutase was increased significantly in liver and kidney, and hence a significant reduction of Malondialdehyde concentration in these organs and blood. According to these findings, it was concluded that the complex formula of Polygonum mulitiflorum Thunb. is better than single formula as a blood lipid lowering agent for those dyslipidemic patients.

目 錄
頁 次
中文摘要………………………………………………………………....I
英文摘要…………………………………………………………….…..II
目錄……………………………………………………………………..IV
表目錄…………………………………………….……………………VII
圖目錄………………………………………….…………..…………...IX
第一章、前言……………………………………………………………1
第二章、文獻回顧………………………………………………………2
第一節、國人血脂現況與脂蛋白概述………………………………2
第二節、血脂異常脂分類與定義……………………………………6
第三節、降血脂中藥…………………………………………………7
（一）降血脂之相關中藥……………………………………..……..7
（二）中藥降血脂的原理機制…………………………………..…..9
第四節、本實驗中藥相關研究………………………………..…….11
（一）何首烏……………………………………………………..….11
（二）黃精………………………………………………………..…15
（三）陳皮…………………………………………………………..17
第五節、自由基簡介…………………………………………….….19
（一）自由基與活性氧的定義……………………………………..19
（二）自由基與活性氧的來源……………………………………..20
（三）自由基與活性氧對生物體之氧化傷害……………………..22
（四）抗氧化劑……………………………………………………..23
第三章、實驗目的……………………………………………………..30
第四章、材料與方法…………………………………………………..31
第一節、實驗材料………………………………………………..…31
第二節、使用試藥………………………………………………..…32
第三節、實驗方法……………………………………………..……34
（一） 基本成分分析………………………………………………....34
（二） 中藥粉末抗氧化能力評估…………………………………....34
（三） 動物實驗流程………………………………………………....39
（四） 生長狀況評估………………………………………………....40
（五） 糞便脂肪代謝評估…………………………………………....40
（六） 血液生化值分析……………………………………………....41
（七） 肝臟功能評估…………………………………………............43
（八） 肝臟脂肪含量分析……………………………………………44
（九） 體內抗氧化能力評估…………………………………………45
（十） 統計分析……………………………………………...……….52
第五章、結果與討論………………………………………………..…53
（一） 中藥基本成分………………………………………………....53
（二） 中藥粉末抗氧化能力評估………………………………..…..53
（三） 生長狀況評估……………………………………………..…..55
（四） 脂肪代謝評估……………………………………………..…..59
（五） 血液生化值分析…………………………………………..…..60
（六） 肝臟功能評估………………………………………………....66
（七） 肝臟脂肪含量分析……………………………………………69
（八） 體內抗氧化能力評估…………………………………………72
第六章、結論…………………………………………………………..82
第七章、參考文獻……………………………………………………..84
附錄……………………………………………………………………..94

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