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研究生:王正文
研究生(外文):Wang Jeng-Wen
論文名稱:FePt和核殼式FePt@Au奈米粒子的製備及性質探討與生醫上之應用
論文名稱(外文):Fabrication and Characterizations of FePt and FePt@Au Nanoparticles for biomedical applications
指導教授:何志松
學位類別:碩士
校院名稱:東海大學
系所名稱:化學工程學系
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:95
中文關鍵詞:鐵鉑奈米粒子核殼式水溶性抗體
外文關鍵詞:FePtaqueousantibody
相關次數:
  • 被引用被引用:4
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近二十年來,奈米粒子被廣泛的應用於生醫研究的領域,藉由奈米粒子不同的磁性、光學或電性的性質來作為偵測、標定及醫療用途。
  本研究是以油相溶液中的化學還原法來製備FePt奈米粒子,以FePt奈米粒子為核,接續合成出核殼式(core-shell) FePt@Au奈米粒子,因為在生醫的應用上必須為水溶性(aqueous),因此在油相中合成,需經過表面修飾,才能使FePt和FePt@Au奈米粒子能夠溶解於水溶液中,本研究使用硫醇族的化合物做表面修飾。更進一步是讓FePt和與streptavidin形成鍵結,在將含有biotin的抗體與FePt-streptavidin混合形成FePt-streptavidin-antibody複合物,此複合物在抗原偵測上可以被有效的使用。
  藉由XRD、TEM、EDS以及VSM實驗分析顯示:奈米核殼複合粒子FePt@Au可被成功的製備,平均粒徑範圍約4~7 nm。此外,從protein label分析中,可以確定streptavidin有與FePt形成鍵結,並利用電泳分析,可在60kD、55kD以及23kD中顯示streptavidin、heavy chain及light chain的訊號,因此我們可以確認FePt-streptavidin-antibody的形成。
In the past decades, nanoparticles are widely studied for the biomedical applications. In terms of their magnetic, optical and electric properties in nanoscale, several technologies have been built for the biological detection, labeling and therapy.
In this study, the oil-phase FePt nanoparticles are synthesized by the chemical reduction method that is one of the most successful technologies for fabricating the monodisperse nanoparticles in solution. Because aqueous-phase nanoparticles are required for the biomedical applications, water-soluble FePt nanoparticles are formed by a thiol-group modification on particle surface. In our experiment, the FePt nanopartices with a 2-3 nm diameter are successfully monodispersed in the hydrophilic solution. Based on the technology of the water-soluble FePt nanoparticles, FePt@Au core-shell nanocrystals with a near 5 nm are made of a scalable chemical reduction method. Then conjugated with streptavidin on the water-soluble FePt nanoparticles and reacted with a biotin-labeled antibody, a FePt-streptavdin-antibody is formed in this study.
The functional FePt nanoparticles are characterized by the XRD, TEM, VSM, EDS, UV-vis spectroscopy and electrophoresis analysis. Their results prove the formation of the water-soluble FePt nasnoparticles, FePt@Au core-shell nanocrystals and FePt-streptavdin-antibody, and are shown in this thesis.
目錄
中文摘要 I
Abstract II
致謝 III
目錄 IV
圖目錄 VII
表目錄 IX
第一章 序論 1
1.1金屬奈米材料在生醫上的應用 1
1.1.1 磁性奈米粒子 1
1.1.2 光學奈米粒子 2
1.1.3 複合材料奈米粒子 14
1-2實驗動機與目的 19
第二章 文獻回顧 20
2.1 FePt奈米粒子的特性 20
2.2 FePt奈米粒子的製備 22
2.3 FePt奈米粒子在生醫的應用 26
2.3.1 單一FePt奈米粒子的應用 26
2.3.2 FePt奈米粒子與其他不同材料結合的應用 27
第三章 磁性奈米粒子的合成 35
3.1前言 35
3.2 製備油溶性FePt奈米粒子 36
3.2.1 實驗的設計 36
3.2.2實驗藥品與儀器 38
3.2.3實驗步驟 40
3.3製備油溶性FePt@Au磁性奈米粒子 43
3.3.1 實驗設計 43
3.3.2實驗藥品與儀器 45
3.3.3 實驗步驟 45
3.4 製備水溶性的FePt磁性奈米粒子 48
3.4.1 實驗設計 48
3.4.2 藥品 54
3.4.3 實驗步驟 54
3.5製備水溶性的FePt@Au磁性奈米粒子 55
3.5.1 實驗設計 55
3.5.2 藥品 56
3.5.3 實驗步驟 56
3.6結果與討論 58
3.6.1 FePt奈米粒子的晶體結構 58
3.6.2 FePt與FePt@Au奈米粒子的粒徑分佈 60
3.6.3 FePt奈米粒子的成分分析 65
3.6.4 FePt及FePt@Au奈米粒子的表面性質 68
3.6.5 FePt及FePt@Au奈米粒子的磁性性質 71
3.6.5 FePt及FePt@Au奈米粒子的光學性質 74
第四章 FePt磁性奈米粒子與生物分子的結合 76
4.1 前言 76
4.2 實驗設計 77
4.2.1 奈米粒子與生物分子的結合 77
4.2.2 分析方法 80
4.3 實驗藥品 82
4.4 實驗步驟 83
4.4.1 將streptavidin修飾在水溶性FePt奈米粒子表面 83
4.4.2 FePt奈米粒子與抗體結合 83
4.5 結果與討論 84
4.5.1 Protein-label分析 84
4.5.1 電泳分析 86
第五章 研究結果與討論 89
第六章 參考文獻 91
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