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研究生:黃萾芬
研究生(外文):Ying-Fen Huang
論文名稱:紅甘藷葉介入對經中強度運動後體內氧化壓力、細胞激素及熱休克蛋白表現之影響
論文名稱(外文):The modulation of purple sweet potato leaves intervention on oxidative stress, cytokines and heat shock protein expression after moderate-intensive exercise
指導教授:劉珍芳劉珍芳引用關係
指導教授(外文):Jen-Fang Liu
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:保健營養學系
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:92
中文關鍵詞:中強度運動氧化壓力細胞激素熱休克蛋白多酚類飲食
外文關鍵詞:moderate-intensity exerciseoxidative stresscytokineheat shock proteinpolyphenol diet
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本研究的主要目的為探討給予含高多酚類飲食的介入,是否可調節進行中強度運動時所造成的氧化壓力及促發炎細胞激素與熱休克蛋白質之表現。
本研究共招募12位一般健康男性為受試者。實驗採交叉試驗,於實驗期間全程均維持低多酚類飲食,實驗之初,受試者先攝取7天低多酚類飲食作為適應期,之後進行兩階段分別為期7天之飲食介入試驗期(高多酚類飲食:紅甘藷葉飲食(PSL)及低多酚類飲食:紅蘿蔔飲食(BC)),兩次飲食介入間隔14天的排空期。於每個實驗階段結束後,受試者需進行70%VO2max的運動1小時,並且收集運動前後的血液樣本及24小時尿液。
實驗結果發現,運動後血漿CK(creatine kinase)、血清8-iso PGF2α (8-iso prostaglandin F2α)、TBARS (thiobarbituric acid reactive substances)及TNF-α(tumor necrosis factor-α)之值與運動前相比較有顯著性升高(p < 0.05),而血漿維生素C、總抗氧化力FRAP (Ferric-reducing antioxidant power)及熱休克蛋白HSP70 (heat shock protein 70)之蛋白質表現量在運動後也有顯著的升高 (p < 0.05)。當給予PSL飲食介入7天後,發現血漿多酚類、維生素E含量及FRAP與BC飲食組相比有顯著性增加(p < 0.05)。此外,經中強度運動後,PSL組的TBARS及TNF-α值顯著低於BC組(p < 0.05)。
本實驗結果發現,當進行1小時70%VO2max的運動時,會造成肌肉損傷、增加體內氧化傷害及HSP70的蛋白質表現量顯著增加;而當給予7天的高多酚類飲食介入後,則可以增加體內的抗氧化狀態及有效降低因中強度運動所造成的氧化壓力及促發炎細胞激素的量,但是對於HSP70的蛋白質表現量則無顯著影響。
The aim of the study was to investigate the effect of high polyphenol diet intervention on the exercise-induced oxidative stress, cytokine excretion and the expression of heat shock protein 72 (HSP72). Twelve non-trained male subjects participated in two-step dietary intervention period. The study was cross-over design, each subject was given high (purple sweet potato leaves, PSL) or low (β-carotene, BC) polyphenol diets for 7 days, respectively. After 14 days of wash-out period, the diet was cross-over. After each dietary intervention period, the subjects ran on a treadmill for 60 minutes at 70% of their peak oxygen consumption. Blood and 24-hr urine were collected for the measurement of muscle damage, oxidative stress, antioxidant status and repaire system parameter. The results indicated that higher plasma CK (creatine kinase), 8-iso PGF2α (8-iso prostaglandin-F2α), TBARS (thiobarbituric acid reactive substances), TNF-α (tumor necrosis factor-α) were found after exercise performing (p<0.05). Also, it showed significant increase in plasma FRAP (Ferric-reducing antioxidant power), vitamin C and HSP70 protein expression (p<0.05). After 7 days of PSL diet intervention, plasma polyphenol content, FRAP and vitamin E significantly increased (p<0.05). In addtion, plasma TBARS and TNF-α had decreased after one bout of moderate-intensity exercise with PSL diet intervention (p<0.05). However, there was no significant difference in HSP70 level between PSL diet and BC diet. The results indicated that PSL diet intervention can increase total antioxidant potency in body and decrease the oxidative stress and cytokine secretion after moderate-intensity exercise, however there was no effect on HSP70 protein expression.
目錄
中文摘要 I
Abstract III
致謝 V
縮寫對照表 VII
目錄 VIII
表目錄 XII
圖目錄 XIII
第一章 前言 1
第二章 文獻回顧 3
第一節 運動時的生理改變 3
ㄧ. 運動強度簡介 3
二. 運動與自由基的產生 5
三. 運動與氧化傷害 8
第二節 熱休克蛋白 10
ㄧ. 熱休克蛋白之簡介 10
二. 熱休克蛋白70家族之分類 10
三. 熱休克蛋白合成之機制 11
四. 運動與熱休克蛋白 15
第三節 蔬菜中多酚類的性質及生理效應 16
ㄧ. 多酚類之化學結構與種類 16
二. 多酚類的食物來源 17
三. 多酚類的生理效用 21
四. 抗氧化物質(多酚類及抗氧化維生素)介入對運動表現之影響 21
第四節 台灣產鄉土蔬菜-紅甘藷葉的特性及生理效用 23
第三章 研究動機與目的 24
第四章 實驗設計與方法 25
第一節 實驗對象招募 25
第二節 實驗材料與製備 26
第三節 實驗設計與流程 28
一. 實驗流程 28
二. 檢體的收集與前處理 31
三. 分析項目 33
第五章 結果 46
第一節 受試者基本資料 46
第二節 進行單次、中強度運動1小時後,受試者體內血液生化數值之變化 48
一. 受試者體內氧化壓力之變化 48
二. 受試者體內促發炎細胞激素之變化 48
三. 受試者體內抗氧化狀態及修復系統指標之變化 49
第三節 攝食紅甘藷葉7天後,受試者體內抗氧化狀態之變化 52
第四節 攝食紅甘藷葉7天後,並進行單次、中強度運動,受試者體內血液生化數值之變化 54
一. 受試者體內肌肉損傷及氧化壓力指標之變化 54
二. 受試者體內促發炎細胞激素之變化 55
三. 受試者體內抗氧化狀態之變化 55
四. 受試者體內修復系統指標之變化 59
第六章 討論 62
第一節 受試者參與實驗前後基本資料之變化 62
第二節 單次、中強度運動對人體血液生化數值之影響 63
一. 肌肉損傷之探討 63
二. 氧化壓力之探討 63
三. 免疫介質之探討 65
四. 抗氧化狀態之探討 66
五. 修復系統指標-HSP70之探討 66
第三節 紅甘藷葉飲食對人體抗氧化狀態之影響 68
第四節 紅甘藷葉飲食介入對於進行單次、中強度運動之影響 70
一. 氧化壓力之探討 70
二. 免疫介質之探討 71
三. 抗氧化狀態之探討 72
四. 修復系統指標-HSP70之探討 73
第七章 結論 74
第八章 參考文獻 75
第九章 附錄 84
林雅玲 (2004) 探討台灣鄉土蔬菜-紅甘藷葉中多酚類之生體可利用率及對人體抗氧化狀態之影響。台北醫學大學保健營養學系碩士論文。

湯淑貞 (2000) 台灣紅色鄉土蔬菜萃取物之抗氧化效力研究。中國文化大學生活應用科學研究所碩士論文。

傅偉光、陳秀瑩、仇志強、陳景川。台灣地區食品成份資料庫。行政院衛生署編印。1998

張文心 (2005) 台灣鄉土蔬菜-紅甘藷葉對運動期之籃球選手其抗氧化力與免疫狀態的調節。台北醫學大學保健營養學系碩士論文。

黃為瑜 (2003) 紅色鄉土蔬菜中類黃酮抗氧化力及其對淋巴求DNA氧化損傷的保護作用。中國文化大學生活應用科學研究所碩士論文。

黃美子 (2006) 台灣鄉土蔬菜-紅甘藷葉粗萃物對人類結直腸癌細胞之影響。台北醫學大學保健營養學系碩士論文。

鄭雅分 (2005) 探討紅甘藷葉對位時炸油老鼠結直腸癌化之效應。台北醫學大學保健營養學系碩士論文。

鐘子雯、陳福財、劉珍芳 (2000) 補充水果之維生素C與中高強度耐力訓練對運動員體內抗氧化狀況之影響。中華民國營養學會雜誌 25:91-98。

Abravaya K, Phillips B and Morimoto RI (1991) Heat shock-induced interactions of heat shock transcription factor and the human hsp70 promoter examined by in vivo footprinting. Mol Cell Biol 11:586-592

Amin J, Ananthan J and Voellmy R (1988) Key features of heat shock regulatory elements. Mol Cell Biol 8:3761-3769

Anderson RF, Amarasinghe C, Fisher LJ, Mak WB and Packer JE (2000) Reduction in free-radical-induced DNA strand breaks and base damage through fast chemical repair by flavonoids. Free Radic Res 33:91-103

Astrand PO, Rodahl K and Van Dalen DB (1986) Testbook of work physiology: physiological basis of exercise. New York: McGraw Hill Book Company 170-175

Benjamin IJ and McMillan DR (1998) Stress (heat shock) proteins: molecular chaperones in cardiovascular biology and disease. Circ Res 83:117-132

Bompa TO (1994) PEROIDIZATION: Theory and Methodology of Training. Human Kinetics pp. 82

Bravo L (1998) Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev 56:317-333

Cao G, Sofic E and Prior RL (1997) Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free Radic Biol Med 22:749-60

Catani MV, Rossi A, Costanzo A, Sabatini S, Levrero M, Melino G and Avigliano L (2001) Induction of gene expression via activator protein-1 in the ascorbate protection against UV-induced damage. Biochem J 356:77-85

Chance B, Sies H and Boveris A (1979) Hydroperoxide metabolism in mammalian organs. Physiol Rev 59:527-605

Chang CK, Huang HY, Tseng HF, Hsuuw YD and Tso TK (2006) Interaction of vitamin E and exercise training on oxidative stress and antioxidant enzyme activities in rat skeletal muscles. J Nutr Biochem 18:39-45

Chang WS, Chen CM, Hu SP, Kan NW, Chieh CC and Liu JF (2007) Effects of purple sweet potato leaves consumption on the modulation of the antioxidative status in basketball players during training period. Asia Pac J Clin Nutr (In press)

Chevion S, Moran DS, Heled Y, Shani Y, Regev G, Abbou B, Berenshtein E, Stadtman ER and Epstein Y (2003) Plasma antioxidant status and cell injury after severe physical exercise. Proc Natl Acad Sci USA 100:5119-23

Chu YH, Chang CL and Hsu HF (2000) Flavonoid content of several vegetables and their antioxidant activity. J Sci Food Agric 80:561-566

Ernster L (1988) Biochemistry of reoxygenation injury. Critical Care Med 16:947-950

Fehrenbach E, Passek F, Niess AM, Pohla H, Weinstock C, Dickhuth HH and Northoff H (2000) HSP expression in human leukocytes modulated by endurance exercise. Med Sci Sports Exerc 32:592-600

Fischer CP, Hiscock NJ, Basu S, Vessby B, Kallner A, Sjoberg LB, Febbraio MA and Pedersen BK (2006) Vitamin E isoform-specific inhibition of the exercise-induced heat shock protein 72 expression in humans. J Appl Physiol 100:1679-1687

Fischer C, Hiscock N, Penkowa M, Basu S, Vessby B and Kallner A (2004) Vitamin C and E supplementation inhibits the release of interlekin-6 from contracting human skeletal muscle. J Physiol 558:633-645

Graefe EU, Wittig J, Mueller S, Riethling AK, Uehleke B, Drewelow B, Pforte H, Jacobasch G, Derendorf H and Veit M (2001) Pharmacokinetics and bioavailability of quercetin glycosides in humans. J Clin Pharmacol 41:492-499

Halliwell B and Gutteridge JMC (1999) Free radicals in biology and medicine. New York: Oxford University Press Inc.

Ioku K, Tsushida T, Takei Y, Nakatani N and Terao J (1995) Antioxidative activity of quercetin and quercetin monoglucosides in solution and phospholipid bilayers. Biochim Biophys Acta 1234:99-104

Jankun J, Selman SH, Swiercz R and Skrzypczak-Jankun E (1997) Why drinking green tea could prevent cancer. Nature. 387:561

Keul J and Doll E (1972) Oxidative energy supply. In: Jokl, E., ed Energy metabolism of human muscle. Basel: Karger 52-71

Khassaf M, McArdle A, Esanu C, Vasilaki A, McArdle F, Griffiths RD, Brodie DA and Jackson MJ (2003) Effects of vitamin C supplements on antioxidant defence and stress proteins in human lymphocytes and skeletal muscle. J Physiol 549:645-652

Kim HY, Kim OH and Sung MK (2003) Effects of phenol-depleted and phenol-rich diets on blood markers of oxidative stress, and urinary excretion of quercetin and kaempferol in healthy volunteers. J Am Coll Nutr 22:217-223

Kregel KC (2002) Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. J Appl Physiol 92:2177-2186

Kuhnau J (1976) The flavonoids. A class of semi-essential food components: their role in human nutrition. World Rev Nutr Diet 24:117-191

Mastaloudis A, Leonard SW and Traber MG (2001) Oxidative stress in athletes during extreme endurance exercise. Free Radic Biol Med 31:911-922

McAnulty SR, McAnulty LS, Nieman DC, Morrow JD, Utter AC, Henson DA, Dumke CL and Vinci DM (2003) Influence of carbohydrate ingestion on oxidative stress and plasma antioxidant potential following a 3 h run. Free Radic Res 37:835-40

McArdle F, Spiers S, Aldemir H, Vasilaki A, Beaver A, Iwanejko I, McArdle A and Jackson MJ (2004) Preconditioning of skeletal muscle against contraction-induced damage: the role of adaptations to oxidants in mice. J Physiol 561:233-244

McCord JM (1988) Free radicals and myocardial ischemia: over review and outlook. Free Rad Biol Med 4:9-16

Moller P, Loft S, Lundby C, Olsen NV (2001) Acute hypoxia and hypoxic exercise induce DNA strand breaks and oxidative DNA damage in humans. FASEB J 15:1181-6

Moon JH, Nakata R, Oshima S, Inakuma T and Terao J (2000) Accumulation of quercetin conjugates in blood plasma after the short-term ingestion of onion by women. Am J Physiol 279:R461-R467

Moran M, Delgado J, Gonzalez B, Manso R and Megias A (2004) Responses of rat myocardial antioxidant defences and heat shock protein HSP72 induced by 12 and 24-week treadmill training. Acta Physiol Scand 180:157-166.

Morrow JD, Hill KE, Burk RF, Nammour TM, Badr KF and Roberts LJ (1990) A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism. Proc Natl Acad Sci 87:9383-9387

Morillas-Ruiz J, Zafrilla P, Almar M, Cuevas MJ, Lopez FJ, Abellan P, Villegas JA, and Gonzalez-Gallego J (2003) The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists. Eur J Appl Physiol 95:543-549

Morillas-Ruiz J, Zafrilla P, Almar M, Cuevas MJ, Lopez FJ, Abellan P, Villegas JA and Gonzalez-Gallego J (2005) The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists. Eur J Appl Physiol 95:543-549

Morillas-Ruiz J, Villegas Garcia JA, Lopez FJ, Vidal-Guevara ML and Zafrilla P (2006) Effects of polyphenolic antioxidants on exercise-induced oxidative stress. Clin Nutr 25:444-453

Morton JP, MacLaren DPM, Cable NT, Bongers T, Griffiths RD, Campbell IT, Evans L, Kayani A, McArdle A and Drust B (2006) Time course and differential response of the major heat shock protein families in human skeletal muscle following acute nondamaging treadmill exercise. J Appl Physiol 101:176-182

Murota K and Terao J (2003) Antioxidative flavonoid quercetin: implication of its intestinal absorption and metabolism. Arch Biochem Biophys 417:12-7

Noble EG, Ho R and Dzialoszynski T (2006) Exercise is the primary factor associated with Hsp70 induction in muscle of treadmill running rats. Acta Physiol 187:495-501

Nourooz-Zadeh J, Cooper MB, Ziegler D and Betteridge DJ (2005) Urinary 8-epi-PGF2α and its endogenous β-oxidation products (2,3-dinor and 2,3-dinor-5,6-dihydro) as biomarkers of total body oxidative stress. Biochem Biophys Res Commun 330:731-736

Orhan H, van Holland B, Krab B, Moeken J, Vermeulen NP, Hollander P and Meerman JH (2004) Evaluation of a multi-parameter biomarker set for oxidative damage in man: increased urinary excretion of lipid, protein and DNA oxidation products after one hour of exercise. Free Radic Res 38:1269-1279

Ostrowski K, Rohde T, Zacho M, Asp S and Pedersen BK (1998) Evidence that interleukin-6 is produced in human skeletal muscle during prolonged running. J Physiol 508:949-953

Overgaard K, Fredsted A, Hyldal A, Ingmann-hansen T, Gissel H and Clausen T (2004) Effects of running distance and training on Ca2+ content and damage in human muscle. Med Sci Sports Exerc 36:821-829

Peake JM, Suzuki K, Hordern M, Wilson G, Nosaka K and Coombes JS (2005) Plasma cytokine changes in relation to exercise intensity and muscle damage. Eur J Appl Physiol 95:514-521

Peake JM, Suzuki K and Coombes JS (2007) The influence of antioxidant supplementation on markers of inflammation and the relationship to oxidative stress after exercise. J Nutr Biochem 18:357-371

Pedersen BK, Saltin B (2006) Evidence for prescribing exercise as therapy in chronic disease. Scand J Med Sci Sports 16:3-63

Pedersen BK, Ostrowski K, Rohde T and Bruunsgaard H (1998) The cytokine response to strenuous exercise. J Physiol Pharmacol 76:505-511

Radak Z, Apor P, Pucsok J, Berkes I, Ogonovszky H, Pavlik G, Nakamoto H and Goto S (2003) Marathon running alters the DNA base excision repair in human skeletal muscle. Life Sci 72:1627-1633.

Ramel A, Wagner KH and Elmadfa I (2004) Correlation between plasma noradrenaline concentrations, antioxidants and neutrophil counts after submaximal resistance exercise in men. Br J Sports Med 38:e22

Ramirez-Tortosa C, Andersen ØM, Gardner PT, Morrice PC, Wood SG, Duthie SJ, Collins AR and Duthie GG (2001) Anthocyanin-rich extract decreases indices of lipid peroxidation and DNA damage in vitamin E-depleted rats. Free Rad Biol Med 31:1033-1037

Rice-Evans C (2001) Flavonoid antioxidants. Curr Med Chem 8:797-807

Ritossa F (1962) A new puffing pattern induced by temperature shock and DNP in Drosophila. Experientia 18:571-573

Sen CK (1995) Oxidants and antioxidants in exercise. J Appl Physiol 79:675-686

Senturk UK, Gunduz F, Kuru O, Kocer G, Ozkaya YG, Yesilkaya A, Bor-Kucukatay M, Uyuklu M, Yalcin O and Baskurt OK (2005) Exercise-induced oxidative stress leads hemolysis in sedentary but not trained humans. J Appl Physiol 99:1434-1441

Snoeckx LH, Cornelussen RN, Van Nieuwenhoven FA, Reneman RS and Van Der Vusse GJ (2001) Heat shock proteins and cardiovascular pathophysiology. Physiol Rev 81:1461-1497

Steensberg A, Morrow J, Toft AD, Bruunsgaard H and Pedersen BK (2002) Prolonged exercise, lymphocyte apoptosis and F2-isoprostanes. Eur J Appl Physiol 87:38-42

Taga MA, Miller EE, Pratt DE (1984) Chia seeds as a source of natural antioxidants. J Am Oil Chem Soc 61:928-931

Tauler P, Gimeno I, Aguilo A, Guix MP and Pons A (1999) Regulation of erythrocyte antioxidant enzyme activities in athletes during competition and short-term recovery. Pflugers Arch 438:782-7


Ting J and Lee AS (1988) Human gene encoding the 78.00-dalton glucoseregulated protein and its pseudogene: structure, conservation, and regulation. DNA 7:275-286

Van Acker FA, Schouten O, Haenen GR, van der Vijgh WJ and Bast A (2000) Flavonoids can replace α-tocopherol as an antioxidant. FEBS Lett 473:145-148.

Vassilakopoulos T, Karatza MH, Katsaounnou P, Kollintza A, Zakynthinos S and Roussos C (2003) Antioxidants attenuate the plasma cytokine response to exercise in humans. J Appl Physiol 94:1025-1032

Von Duvillard SP, Braun WA, Markofski M, Beneke R and Leithauser R (2004) Fluids and hydration in prolonged endurance performance. Nutrition 20:651-656

Weiss SJ and LoBuglio AF (1982) Phagocyte-generated oxygen metabolite and cellular injury. Lab Invest 47:5-10

Welch WJ (1992) Mammalian stress response: cell physiology, structure/function of stress proteins, and implications for medicine and disease. Physiol Rev 72:1063-1081

Welch WJ (1993) Heat shock proteins functioning as molecular chaperones: their roles in normal and stressed cell. Phil Trans R Soc Lond B Biol Sci 339:327-333

Westman J, Drieu K and Sharma HS (2000) Antioxidant components EGB-761 and BN-56021 attenuate heat shock protein (HSP72 kD) response, edema and cell changes following hyperthermic brain injury. Amino Acids 19:339-350

Williams RJ, Spencer JPE and Rice-Evans C (2004) Serial review: flavonoids and isoflavones (phytoestrogens): absorption, metabolism, and bioactivity Free Radic Biol Med 36:838-849

Yang CS, Landau JM, Huang MT and Newmark HL (2001) Inhibition of carcinogenesis by dietary polyphenolic compounds. Annu Rev Nutr 21:381-406

Zala CA, Salas-Prato M, Yan WT, Banjo B and Perdue JF (1980) In cultured chick embryo fibroblasts the hexose transport components are not the 75.000 and 95.000 dalton polypeptides synthesized following glucose deprivation. Can J Biochem 58:1179-1188
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