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研究生:詹芷琪
研究生(外文):Chih-Chi Chan
論文名稱:松杉靈芝之補充對大鼠之運動表現及力竭運動後抗氧化狀態之影響
論文名稱(外文):Effects of Ganoderma tsugae supplementation on exercise performance and antioxidant status after exhaustive exercise in rats
指導教授:楊素卿楊素卿引用關係
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:保健營養學研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:105
中文關鍵詞:松杉靈芝力竭運動運動表現細胞凋亡
外文關鍵詞:Ganoderma tsugaeexhaustive exerciseexercise performanceapoptosis
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本研究之目的為探討松杉靈芝之補充對大白鼠因力竭運動所導致的抗氧化狀態不平衡及其運動表現之影響。利用三十隻雄性Sprague-Dawley大白鼠,經一週適應期後,跑步機適應較佳的二十四隻,以隨機方式分為力竭運動(E)、力竭運動補充低劑量(0.1875 g/kg BW)(EL)、中劑量(0.9375 g/kg BW)(EM)及高劑量(1.875 g/kg BW)松杉靈芝(EH)等四組,其餘六隻則為控制組(C)。經三十天餵養後,控制組大白鼠立即犧牲,運動之四組大白鼠以跑步機經力竭運動後予以犧牲,採集其血液、肌肉及肝臟樣本。結果發現,能量代謝方面,與C組相較,E組之肌肉肝醣含量、血糖濃度顯著降低、血尿素氮濃度顯著增加(p < 0.05)。而與E組相較之下,EL組血中游離脂肪酸濃度顯著降低,EM組血糖濃度及肝醣含量顯著降低,而EH組肝醣含量顯著降低(p < 0.05),其餘數據皆無顯著差異。跑步時間方面,EL組、EH組較E組顯著增加(p < 0.05),且EH組又比EL組顯著增加(p < 0.05),而EM組只有增加之趨勢。抗氧化狀態方面,E組之紅血球SOD(superoxide dismutase)、CAT(catalase)、GRD(glutathione reductase)活性及GSH/GSSG比值與C組相較顯著降低(p < 0.05),而EL、EM及EH組其SOD、CAT、GPX(glutathione peroxidase)、GRD活性及GSH/GSSG與E組則無顯著差異,但EH組血漿TBARS濃度則顯著低於E組(p < 0.05)。組織損傷方面,E組血漿中AST(aspartate transaminase)、ALT(alanine transaminase)、CK(creatine kinase)及LDH(lactate dehydrogenase)活性顯著高於C組(p < 0.05),肌肉細胞凋亡情形也較嚴重。與E組相較,EL、EM及EH組其AST、ALT、CK及LDH活性皆無顯著差異,肌肉細胞凋亡情形則有較低的現象。以上結果顯示,大白鼠力竭運動前補充低劑量(0.1875 g /kg BW)松杉靈芝,可維持力竭運動後其血糖恆定及肌肉肝醣含量,並減少肌肉細胞凋亡的情形,而補充高劑量(1.875 g /kg BW)松杉靈芝,除上述作用外,更可增進脂肪利用及減少脂質過氧化物產生,以達延長運動時間之效果。
The purpose of this study was to investigate the effects of Ganoderma tsugae (GT) supplementation on exercise-induced imbalance of antioxidative status and exercise performance in rats. Thirty male Sprague-Dawley rats were divided into five groups: control (C), exhaustive exercise (E), exhaustive exercise with low dose (0.1875 g/kg BW) (EL), middle dose (0.9375 g/kg BW) (EM) and high dose (1.875 g/kg BW) GT supplementation (EH) groups. After 30 days, the rats of exercise groups underwent an acute exhaustive running test on a motorized treadmill. After the running test, all rats were sacrificed immediately. The blood, muscle and liver samples were collected. The energy metabolism, the muscle glycogen content and blood glucose were significantly decreased and blood urea nitrogen (BUN) was increased in E group (p<0.05), compared with C group. Compared with E group, the free fatty acids (FFA) concentration in EL group, blood glucose concentration in EM group, and the liver glycogen content in EM and EH group were significantly decreased (p<0.05). On the other hand, the running time was obviously prolonged in EL and EH group than that of E group (p<0.05), and it significantly in EH group than that of EL group (p<0.05). For the antioxidative status, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GRD) and GSH/GSSG ratio in the erythrocytes were obviously reduced (p<0.05). There were no significant differences in SOD, CAT, glutathione peroxidase, GRD activities, and GSH/GSSG ratio in EL, EM and EH group, when compared with E group. TBARS level of EH group was apparently decreased than that of E group (p<0.05). About the tissue damage, the activities of aspartate transaminase (AST), alanine transaminase (ALT), creatine kinase (CK) and lactate dehydrogenase (LDH) in the plasma were significantly raised in E group (p<0.05), when compared with C group (p<0.05). The apoptosis of muscle cell in E group was more apparent than in C group. The plasma AST, ALT, CK and LDH activities in EL, EM, and EH group had no changes, when compared with E group. The apoptosis of muscle cell in EL, EM, and EH group was less apparent than in E group. The results show that low dosage supplementation of Ganoderma tsugae before exhaustive exercise may maintain glucose homeostasis, muscle glycogen content and attenuate apoptosis of muscle cell to prolong running time.
中文摘要 I
英文摘要 III
致謝 V
目錄 IX
表目錄 XIII
圖目錄 XIV
第一章 研究動機與目的 1
第二章 文獻回顧 3
第一節 運動時之生理變化 3
第二節 運動與能量代謝 3
第三節 運動與疲勞 5
(一)中樞疲勞機制 6
(二)週邊疲勞機制 7
第四節 運動與氧化壓力 9
(一)運動造成氧化壓力之可能機轉 9
(二)運動與抗氧化狀態之關係 13
(三)運動與組織傷害之關係 14
第五節 靈芝 15
(一)靈芝之簡介 15
(二)靈芝之化學活性成分 17
第三章 材料與方法 24
第一節 實驗材料及儀器 24
(一)手術用藥 24
(二)飼料成分 24
(三)分析儀器設備 24
第二節 實驗方法 26
(一)實驗動物分組與飼料 26
(二)松杉靈芝之投予方式與劑量 27
(三)大白鼠適應與單次激烈力竭運動 29
(四)動物犧牲流程 32
(五)樣本採集及前處理 33
第三節 分析項目與方法 36
(一)分析項目 36
(二)測定方法 38
第四節 統計分析 53
第四章 結果 54
第一節 體重與攝食量 54
第二節 跑步時間 56
第三節 能量代謝 57
(一)血糖濃度 57
(二)血乳酸濃度 57
(三)游離脂肪酸濃度 57
(四)血尿素氮濃度 58
(五)肌肉肝醣與肝臟肝醣含量 58
第四節 抗氧化狀態 61
(一)抗氧化酵素活性 61
(二)抗氧化物質指標 61
(三)脂質過氧化濃度 61
第五節 組織傷害 63
第六節 組織細胞凋亡染色 64
第五章 討論 67
第一節 體重與攝食量 67
第二節 能量代謝及運動表現 68
(一)運動與能量代謝 68
(二)松杉靈芝補充對跑步時間及能量代謝之影響 69
第三節 抗氧化狀態 72
(一)運動與抗氧化狀態 72
(二)松杉靈芝補充對抗氧化狀態之影響 73
第四節 組織傷害 74
(一)運動與組織傷害 74
(二)松杉靈芝補充對組織傷害之影響 75
第五節 細胞凋亡 76
(一)運動與細胞凋亡 76
(二)松杉靈芝補充對細胞凋亡之影響 77
第六節 松杉靈芝補充劑量 78
第七節 活性成分 79
第六章 結論 80
參考文獻 81
附錄 88
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