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研究生:周士會
研究生(外文):Shih-Hui Chou
論文名稱:添加菊苣糖之綠茶對大白鼠腸道菌相之影響
論文名稱(外文):Effect of inulin-containing green tea on intestinal microflora in rats
指導教授:陳俊榮陳俊榮引用關係
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:保健營養學研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:70
中文關鍵詞:綠茶菊苣糖乳酸桿菌雙叉桿菌
外文關鍵詞:green teainulinLactobacillusBifidobacterium
相關次數:
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本研究的目的在探討含菊苣糖之綠茶飲料,對於大白鼠腸道菌相以及消化酵素活性的影響。本研究以24隻六週齡大之雄性SD大白鼠為實驗對象,在預備飼養後,隨機分為三組,每組8隻。對照組給予正常飼料,實驗組則分別添加1.5或3.0 g/kg/day之菊苣糖綠茶粉末於飼料中。於第4週及第8週檢測其糞便中之厭氧細菌總數、乳酸桿菌、雙叉桿菌、及大腸桿菌。於第8週時犧牲動物,檢測其空腸中脂解酶及雙醣酶之酵素活性。試驗結果發現:攝取低劑量菊苣糖綠茶粉末8週後,實驗動物糞便中的乳酸桿菌菌數顯著增加 (p < 0.05)。攝取高劑量菊苣糖綠茶粉末組,於實驗第4週後,乳酸桿菌菌數即有顯著增加(p < 0.05)。但是,菊苣糖綠茶粉末對於糞便中的雙叉桿菌菌數則無顯著的影響。另外,不論攝取低劑量或高劑量的菊苣糖綠茶粉末,在實驗後第4和8週均可顯著降低糞便中大腸桿菌菌數(p < 0.05)。攝取高劑量的菊苣糖綠茶粉末可以顯著增加大白鼠空腸前段中脂解酶和蔗糖酶等消化酵素活性(p < 0.05)。綜合以上結果,在飼料中添加菊苣糖綠茶粉末,不僅可以增加實驗動物腸道內益生菌、抑制有害菌,改善腸道菌相平衡,亦可增加空腸黏膜消化酵素活性,有助於腸道健康。
The purpose of this study was to investigate the effects of green tea beverage contain inulin on the intestinal microflora ecology and digestive enzymes in rats. Twenty-four male Sprague-Dawley rats were randomly divided into three groups : control (C), low (L) and high dosage (H) groups (1.50 and 3.0 g/kg BW/day green tea beverage powder were added to the diet of L and H groups, respectively). The green tea beverage powder was made from the green tea beverage containing inulin after freeze-dried. Fecal samples were collected at week 4 and 8, and analyzed for intestinal microflora. Jejunal samples were collected at week 8, and analyzed for digestive enzymes. The rats in H group had higher fecal Lactobacillus and lower E. coli counts at both week 4 and 8, than those in C group (p < 0.05). Rats in L group had higher fecal Lactobacillus at week 8 and lower E. coli counts at week 4 and 8, than those in C group (p < 0.05). In addition, the jejunal lipase and lactase activities were significantly increased in H group (p < 0.05), but not in L group. In conclusion, our results suggested that high dosage of green tea beverage powder not only modulated the intestinal microflora ecology but also increased the digestive enzymes activities in rats.
目錄
摘 要 I
Abstract II
誌謝 III
目錄 IV
第一章 緒言 1
第二章 文獻回顧 4
第一節 腸道微生物 4
一、腸道內微生物分布 4
二、腸道菌叢對人體健康的影響 6
第二節 益生菌 8
一、益生菌的定義 8
二、乳酸菌定義及分類 9
三、益生菌的種類 10
四、益生菌的特質 11
五、益生菌對胃腸道的健康效應 12
第三節 益生質 15
一、益生質的定義 15
二、益生質的特質 15
三、菊苣糖 16
第四節 茶 18
一、茶的簡介 18
二、茶的功效成分 19
三、綠茶 20
四、茶的健康效應 21
第三章 實驗設計與方法 25
第一節 實驗架構 25
第二節 實驗設計與方法 27
一、實驗動物及飼養方法: 27
二、飼料內容 27
三、菊苣糖綠茶粉末投與量之推算 28
第三節 檢測項目及方法 29
一、菌相測定 29
二、空腸消化酵素活性測定 32
第四節 統計分析 34
第四章 實驗結果 35
第一節 飼料攝取量及體重變化 35
第二節 腸道菌相 36
一、乳酸桿菌菌落數及雙叉桿菌菌落數 36
二、厭氧菌總菌落數 36
三、大腸桿菌菌落數 36
第三節 空腸脂解酶及雙醣酶之活性 38
一、脂解酶之活性 38
二、雙醣酶之活性 38
第五章 討論 40
第一節 攝食量、體重變化 40
第二節 腸道菌相 41
第三節 消化酵素活性 44
第六章 結論 46
第七章 參考文獻 47



表目錄
表一、Laboratory rodent diet 5001組成..................58
表二、各組粉末飼料組成.................................61
表三、各組粉末飼料之攝取量.............................62
表四、菊苣糖綠茶粉末對大白鼠體重的影響.................63
表五、菊苣糖綠茶粉末對大白鼠糞便重量以及含水量的影響...64
表六、菊苣糖綠茶粉末對大白鼠糞便中乳酸桿菌、雙叉桿菌、
厭氧菌、總數及大腸桿菌之影響……………………65
表七、菊苣糖綠茶粉末對大白鼠空腸脂解酶及雙醣酶活性的影響…66


圖目錄
圖一、嬰兒於出生至七天內腸道菌相之情形...............67

圖二、腸道菌相隨年齡改變之情形.......................68
圖三、菊苣糖(inulin)結構式.......................... 69
圖四、兒茶素的化學結構分類...........................70
Axelsson L. (1998) Lactic Acid Bacteria: Microbiology and Functional Aspects. 2nd edition, pp. 1-72.

Ben XM, Zhou XY, Zhao WH, Yu WL, Pan W, Zhang WL, Wu SM, Van Beusekom CM and Schaafsma A. (2004) Supplementation of milk formula with galacto-oligosaccharides improves intestinal micro-flora and fermentation in term infants. Chin Med J. 117:927-31.

Bouhnik Y, Flourie B, Andrieux C, Bisetti N, Briet F and Rambaud JC. (1996) Effects of Bifidobacterium sp fermented milk ingested with or without inulin on colonic bifidobacteria and enzymatic activities in healthy humans. Eur J Clin Nutr. 50:269-73.

Bouhnik Y, Raskine L, Simoneau G, Vicaut E, Neut C, Flourie B, Brouns F and Bornet FR. (2004) The capacity of nondigestible carbohydrates to stimulate fecal bifidobacteria in healthy humans: a double-blind, randomized, placebo-controlled, parallel-group, dose-response relation study. Am J Clin Nutr. 80:1658-64.

Campieri M and Gionchetti P. (1999) Probiotics in inflammatory bowel disease: new insight to pathogenesis or a possible therapeutic alternative? Gastroenterology. 116:1246-60.

Collins MD and Gibson GR. (1999) Probiotics, prebiotics, and synbiotics: approaches for modulating the microbial ecology of the gut. Am J Clin Nutr. 69:1052s-7s.

Crespy V and Williamson G. (2004) A review of the health effects of green tea catechins in in vivo animal models. J Nutr. 134:3431s-40s.

Cummings JH and Macfarlane GT. (1997) Role of intestinal bacteria in nutrient metabolism. Clin Nutr. 16:3-11.

Cummings JH, Macfarlane GT and Englyst HN. (2001) Prebiotic digestion and fermentation. Am J Clin Nutr. 73:415s-20s.

Dai J, Wang HX, Chen SW and Tang J. (2001) Study on the analytical methods of catechins in tea and green tea polyphenol samples by high performance liquid chromatography. Se Pu. 19:398-402.

Delzenne NM, Kok N, Deloyer P and Dandrifosse G. (2000) Dietary fructans modulate polyamine concentration in the cecum of rats. J Nutr. 130:2456-60.


Djouzi Z, Andrieux C, Degivry M-C, Bouley C and Szylit O. (1997) The association of yogurt starters with lactobacillus casei DN 11.001 in fermented milk alters the composition and metabolism of intestinal microflora in germ-free rats an in human flora-associated rats. J Nutr. 127:2260-6.

Fanaro S, Jelinek J, Stahl B, Boehm G, Kock R and Vigi VJ. (2005) Acidic oligosaccharides from pectin hydrolysate as new component for infant formulae: effect on intestinal flora, stool characteristics, and pH. Pediatr Gastroenterol Nutr. 41:186-90.

FAO/WHO. (2001) Evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Report of a Joint FAO/WHO Expert Consltation. Available at: http://www.fao.org/es/ESN/food/foodandfoo_probio_en.stm.

Fooks LJ and Gibson G.R. (2002) Probiotics as modulators of the gut flora. Br J Nutr. 88(1): s39-s49.

Fooks LJ, Fuller R and Gibson GR. (1999) Prebiotics, probiotics and human gut microbiology. Int Dairy J. 9:53-61.

Fuller R. (1989) Probiotics in man and animals. J Appl Bacteriol. 66: 365-378.
Furrie E, Macfarlane S, Kennedy A, Cummings JH, Walsh SV, O''neil DA and Macfarlane GT. (2005) Synbiotic therapy (Bifidobacterium longum/Synergy 1) initiates resolution of inflammation in patients with active ulcerative colitis: a randomised controlled pilot trial. Gut. 54:242-9.

Gibson GR. (1999) Dietary modulation of the human gut microflora using the prebiotics oligofructose and inulin. J Nutr. 129: 1438s-41s.

Gibson GR and Roberfroid MB. (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr. 125: 1401-1412.
Gibson GR, Beatty ER, Wang X and Cummings JH. (1995) Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology. 108:975-82.

Gibson GR, Probert H, Van Loo J, Rastall R and Roberfroid MB. (2004) Dietary modulation of the human colonic microbiota: updating the concept of prebiotics. Nutr Res Rev. 17:259-275.

Ginsberg DI, Drapeau C and Jensen GS. (2000) Probiotic bacteria and the immune system. JANA. 3:44-50.

Goldin BR. (1998) Health benefits of probiotics. Br J Nutr. 80(2):s203-s207.

Gorbach SL. (1990) Lactic acid bacteria and human health. Ann Med. 22:37-41.

Green tea. (2000) Altern Med Rev. 5:372-5.

Guarner F and Malagelada JR. (2003) Gut flora in health and disease. Lancet 361:512-9.

Hara Y. (1997) Influence of tea catechins on the digestive tract. J Cell Biochem. 27:52-8.

Havenaar R and Huis In''t Veld JHJ. (1992) Probiotics: a general view. In: Wood BJB,editor. The lactic acid bacteria in health and disease. Amsterdam: Elsevier. 1-200.

Hirasawa M, Takada K and Otake S. (2006) Inhibition of acid production in dental plaque bacteria by green tea catechins. Caries Res. 40:265-70.

Holzapfel WH, Haberer P, Snel J, Schillinger U and Huis In''t Veld JHJ. (1998) Overview of gut flora and probiotics. Inter J Food Micro. 41: 85-101.

Huang SW and Frankel EN. (1997) Antioxidant activity of tea catechins in different lipid systems. J Agric Food Chem. 45:3033-8.

Ikeda I, Tsuda K, Suzuki Y, Kobayashi M, Unno T, Tomoyori H, Goto H, Kawata Y, Imaizumi K, Nozawa A and Kakuda T. (2005) Tea catechins with a galloyl moiety suppress postprandial hypertriacylglycerolemia by delaying lymphatic transport of dietary fat in rats. J Nutr. 135:155-9.

Ikeda M, Suzuki C, Umegaki K, Saito K, Tabuchi M and Tomita T. (2007) Preventive effects of green tea catechins on spontaneous stroke in rats. Med Sci Monit. 13:40-5.

Ishibashi N and Shimamura S. (1993) Bifidobacteria: Research and development in Japan. Food Technol. 47: 126-135.

Jenkins DA, Kendall CWC and Vuksan V. (1999) Inulin, oligofructose and intestinal function. J Nutr. 129:1431s-3s.

Kao YH, Hiipakka RA and Liao S. (2000a) Modulation of endocrine systems and food intake by green tea epigallocatechin gallate. Endocrinology. 141:980-7.

Kao YH, Hiipakka RA and Liao S. (2000b) Modulation of obesity by a green tea catechin. Am J Clin Nutr. 72:1232-4.

Kasper H. (1998) Protection against gastrointestional diseases- present facts and future developments. Inter J Food Micro. 41:127-31.

Kondo K, Kurihara M, Miyata N, Suzuki T and Toyoda M. (1999) Mechanistic studies of catechins as antioxidants against radical oxidation. Arch Biochem Biophys. 362:79-86.

Koo MWL and Cho CH. (2004) Pharmacological effects of green tea on the gastrointestinal system. Eur J Pharmacol. 500:177-85.

Langlands SJ, Hopkins MJ, Coleman N and Cummings JH. (2004) Prebiotic carbohydrates modify the mucosa associated microflora of the human large bowel. Gut. 53:1610-6.

Liao S, Umekita Y, Guo J, Kokontis JM, and Hiipakka RA. (1995) Growth inhibition and regression of human prostate and breast tumors in athymic mice by tea epigallocatechin gallate. Cancer Lett. 96:239-43.

Lilly DM and Stillwell RH. (1965) Probiotics: growth promoting factors produced by microorganisms. Science. 147:747-748.

Mai VHA, Katki H, Harmsen D, Gallaher A, Schatzkin D, Baer J and Clevidence B. (2004) Effects of a controlled diet and black tea drinking on the fecal microflora composition and the fecal bile acid profile of human volunteers in a double-blinded randomized feeding study. J Nutr. 134:473-8.

Makras L, Van Acker G and De Vuyst L. (2005) Lactobacillus paracasei subsp. paracasei 8700:2 degrades inulin-type fructans exhibiting different degrees of polymerization. Appl Environ Microbiol. 71:6531-6537.

Mitsuoka T. (1992) Intestinal flora and aging. Nutr Rev. 50: 438-446.

McCracken VJ and Lorenz RG. (2001) The gastrointestinal ecosystem: a precarious alliance among epithelium, immunity and microbiota. Cell Microbiol. 3:1–11.

Metchnikoff E. (1907) The prolongation of life. Willian Heinemann, London, UK.

Meydani SN and Ha WK. (2000) Immunologic effects of yogurt. Am J Clin Nutr. 71:861-72.
Mizuno H, Cho YY, Zhu F, Ma WY, Bode AM, Yang CS, Ho CT and Dong Z. (2006) Theaflavin-3, 3''-digallate induces epidermal growth factor receptor downregulation. Mol Carcinog. 45:204-12.

Nakahara K, Kawabata S, Ono H, Ogura K, Tanaka T, Ooshima T and Hamada S. (1993) Inhibitory effect of oolong tea polyphenols on glycosyltransferases of mutans Streptococci. Appl Environ Microbiol. 59:968-73.

Noack J, Klessen B, Proll J, Dongowski G and Blaut M. (1998) Dietary guar gum and pectin stimulate intestinal microbial polyamine synthesis in rats. J Nutr. 128:1385-1391.

Osadaa K, Takahashia M, Hoshinaa S, Nakamura M, Nakamura S and Sugano M. (2001) Tea catechins inhibit cholesterol oxidation accompanying oxidation of low density lipoprotein in vitro. Comp Biochem Physiol. 128:153-64.

Parker RB. (1974) Probiotics, the other half of the antibiotic story. Anim Nutr Health. 29:4-8.

Pochart P, Marteau P, Bouhnik Y, Goderel I, Bourlioux P and Rambaud JC. (1992) Survival of bifidobacteria ingested via fermented milk during their passage through the human small intestine: an in vivo study using intestinal perfusion. Am J Clin Nutr. 55:78-80.

Raederstorff DG, Schlachter MF, Elste V and Weber P. (2003) Effect of EGCG on lipid absorption and plasma lipid levels in rats. J Nutr Biochem. 14:326-32.

Rao AV. (1999) Dose-pesponse effects of insulin and oligofructose on intestinal bifidogenesis. J Nutr. 129:1442s-5s.

Roberfroid MB. (1998) Prebiotics and synbiotics: concepts and nutritional properties. Br J Nutr. 80:197-202.
Roberfroid MB, Van Loo JAE and Gibson GR. (1998) The bifidogenic nature of chicory inulin and its hydrolysis products. J Nutr. 128:11-19.

Roberfroid M. (1993) Dietary fiber, inulin, and oligofructose: a review comparing their physiological effects. Crit Rev Food Sci Nutr. 33:103-48.

Rolfe RD. (2000) The role of probiotic cultures in the control of gastrointestinal health. J Nutr. 130:396s-402s.

Ross RP, S Morgan and C Hill.(2002) Preservation and fermentation: past, present and future. Inter J Food Micro. 79:3-16.

Sanders ME, Walker DC, Walker KM, Aoyama K and Klaenhammer TR. (1996) Performance of commercial cultures in fluid milk applications. J Dairy Sci. 79:943-55.

Satink HP, Hessels J, Kingma AW, VandenBerg GA, Muskiet FA and Halie MR. (1989) Microbial influences on urinary polyamine excretion. Clin Chim Acta. 179:305-314.

Schrezenmeir J and Vrese M. (2001) Pobiotics, prebiotics, and synbiotics-approaching a definition. Am J Clin Nutr. 73:361s-4s.

Simon GL and Gorbach SL. (1982) Intenstional microflora. Ned Clin North Am. 66:557-74.

Song JM, Lee KH and Seong BL. (2005) Antiviral effect of catechins in green tea on influenza virus. Antiviral Res. 68:66-74.

Suzuki Y, Unno T, Kobayashi M, Nozawa A, Sagesaka Y and Kakuda T. (2005) Dose-dependent suppression of tea catechins with a galloyl moiety on postprandial hypertriglyceridemia in rats. Biosci Biotechno Biochem. 69:1288-91.

Toure R, Kheadr E, Lacroix C, Moroni O and Fliss I. (2003) Production of antibacterial substances by bifidobacterial isolates from infant stool active against Listeria monocytogenes. J Appl Microbiol. 95:1058-69.

Uchida S, Ozaki M, Akashi T, Yamashita K, Niwa M and Taniyama K. (1995) Effects of (-)-epigallocatechin-3-O-gallate (green tea tannin) on the life span of stroke-prone spontaneously hypertensive rats. Clin Exp Pharmacol Physiol Suppl. 22:S302-3.

Vrese M, Stegelmann A, Richter B, Fenselau S, Lauem C and Schrezenmeir J. (2001) Probiotics- compensation for lactase insufficiency. Am J Clin Nutr. 73:421s-9s.

Wolfram S, Wang Y and Thielecke F. (2006) Anti-obesity effects of green tea: from bedside to bench. Mol Nutr Food Res. 50:176-87.

Xu JZ, Yeung SY, Chang Q, Huang Y and Chen ZY. (2004) Comparison of antioxidant activity and bioavailability of tea epicatechins with their epimers. Br J Nutr. 91:873-81.

Yamamoto M, Miyamoto S, Moon JH, Murota K, Hara Y and Terao J. (2006) Effect of dietary green tea catechin preparation on oxidative stress parameters in large intestinal mucosa of rats. Biosci Biotechnol Biochem. 70:286-9.

Yukuchi H, Goto T and Okong S. (1992) Fermented milks, lactic drunks and intestinal microflora, In “Functions of Fermented Milk”, Y. Nakazawa and A. Hosono(Eds), Translate by Howells B. W. Elsevier appl Sci. NY.

中華民國93年國民醫療保健支出統計。行政院衛生署衛生統計資訊網。http://www.doh.gov.tw/statistic/data/國民醫療保健支出/93/9305.xls。

中華民國94年全民健康保險醫療統計年報。行政院衛生署衛生統計資訊網。http://www.doh.gov.tw/statistic/醫療統計年報/94.htm。

丘志威、陳政雄(2001)乳酸菌具保健功效之有效成分探討成果報告。pp101-116。衛生署。

苗明三(1997)實驗動物和實驗動物技術。pp.127-132。中國人民出版社。

陸羽(1965)茶經。百部從書籍成,百川學海第二函。藝文印書館出版。

陳右人(1998)茶樹品種與育種介紹。茶葉技術推廣手冊茶作篇。台灣省茶業改良場編印。p.7-14。
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7. 胡庭禎、王朝明(2000)。〈全民健康保險實施後醫院社會工作人員角色轉變之初探〉。《醫院》,1.33(5)。
8. 胡庭禎(1999)。〈組織承諾與專業生涯承諾之探討一以護理人員為例〉。《弘光學報》,33:1-19。
9. 周鴻儒、陳淑樺、蕭世榮、王蓉敏(2001)。〈醫院員工之工作滿意度及組織承諾調查-以某家地區醫院為例〉。《醫院》,34(6):45-53。
10. 朱金池(1991)。〈影響組織承諾因素之研究〉。《警政學報》,19:111-128。
11. 王存國、戴基峰(2001)。〈組織特性與資訊科技對組織結構之影響〉。《資訊管理學報》,7(2):1-26。
12. 張瑞當、徐漢祥、倪豐裕(2001)。〈公平性認知對組織成員工作滿意度與組織承諾之實證研究〉。《中山管理評論》,9(1):135-163。
13. 莫藜藜(1992)。〈醫務社會工作在台灣的現況與發展〉。《社區發展季刊》,60:3-11。
14. 莫藜藜(1996)。〈健保實施後對醫務社會工作的衝擊-社會工作的因應與角色轉型初探〉。《社區發展季刊》,76:91-100。
15. 陳玉枝(2005)〈公立醫院委託公立機構經營後員工對組織文化及組織承諾關聯性之探討〉。《醫院》,1.38(5):1-16。