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研究生:陳麗靜
研究生(外文):Li-Ching Chen
論文名稱:孔雀綠用於吳郭魚藥浴時對魚體殘留性之探討
論文名稱(外文):Studies on the residual malachite green in tilapia during the waterborne exposure period
指導教授:黃加成黃加成引用關係
指導教授(外文):Chia-Cherng Huang
學位類別:碩士
校院名稱:台南科技大學
系所名稱:生活應用科學研究所
學門:民生學門
學類:生活應用科學學類
論文種類:學術論文
論文出版年:2007
畢業學年度:95
語文別:中文
論文頁數:91
中文關鍵詞:孔雀綠還原型孔雀綠吳郭魚
外文關鍵詞:malachite greenleucomalachite greentilapia
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本研究旨在探討吳郭魚藥浴孔雀綠,於不同藥浴濃度、魚隻密度及水環境等條件下,魚體中孔雀綠及還原型孔雀綠殘留之情形,以供衞生品質監測之參考。其研究結果顯示如下:
試驗一:分別以0.1、0.5及1.0 mg / L等孔雀綠濃度進行藥浴,一天後更換至不含孔雀綠之海水。藥浴初期,試驗組魚體中孔雀綠蓄積量隨藥浴濃度及時間的增加而增加,直到藥浴第8小時,其蓄積濃度達最高,之後隨時間的延長而降低。孔雀綠殘留量分別在換水後16天、40天及56天,而還原型孔雀綠則分別於40天、64天及80天降達1 μg / kg以下。
試驗二:以0.1 mg / L孔雀綠濃度分別對三種不同魚隻密度 (60隻 / 200 L、30隻 / 200 L及15隻 / 200 L) 進行藥浴,一天後更換至不含孔雀綠之海水。藥浴期間,蓄養密度與魚體中孔雀綠及還原型孔雀綠之蓄積量成反比。換水後第6天,各組之蓄養密度與孔雀綠及還原型孔雀綠總殘留量無相關性。
試驗三:以0.1 mg / L孔雀綠濃度分別對三種不同水環境 (純海水、半鹹海水及淡水) 進行藥浴,一天後分別更換至不含孔雀綠之水環境。藥浴期間,純海水組魚體孔雀綠及還原型孔雀綠蓄積量顯著高於半鹹海水及純淡水組者。換水後第6天,純淡水組魚體孔雀綠及還原型孔雀綠之總殘留量反而顯著高於半鹹海水組及純海水組。
Three experiments of different malachite green (MG) (0.1, 0.5 or 1.0 mg/L) waterborne exposure were associated with different reared density (60, 30 or 15 fish/200 L) or environment (sea, brackish or fresh water) to investigate the accumulative concentration of MG and leucomalachite green (LMG) when the waterborne exposure, or residual concentration of MG and LMG after transferred promptly into clean water in tilapia.
1. This experiment focused on the fish was exposed to different MG levels (0.1, 0.5 and 1 mg/L) for 24 h. The result shown that the accumulative concentration of muscular MG from any treatment was significant increased with the concentration and time of MG through a waterborne exposure. The accumulative concentration of muscular MG reached the highest at 8th h during the waterborne exposure. Then the residual concentration of muscular MG was reduced follows reared time. The muscular MG residues of fish muscle were below 1μg / kg when the fish were reared up to 16th, 40th and 56th day, and the muscular LMG residues were 40th , 64th and 80th day.
2. This experiment focused on three different reared densities (15, 30 or 60 fish/200 L) and the fish were exposed to MG at 0.1 mg/L for 24 h. The results were observed that the accumulative concentration of muscular MG and LMG from the trial fish of lowest reared density (15 fish/200 L) were significant higher than those from the highest reared density. The trial fish were transferred promptly into clean water by the end of MG waterborne exposure. The correlation between reared densities and MG concentration at 6th day were insignificant.
3. This experiment focused on three different reared environments (sea, brackish or fresh-water) and the fish were exposed to MG at 0.1 mg / L. The result shown that the accumulative concentration of muscular MG and LMG of the fish from sea water were significant higher than those from brackish or fresh water. The total or any concentration of muscle MG and LMG residues from sea water group were lower than those from brackish or fresh water group, when the fish were transferred to clean water and past 6 day.
壹、中文摘要…………………………………………………….………………. 1
貳、前言………………………………………………………….………………. 2
參、文獻檢討…………………………………………………….………………. 4
一、吳郭魚在台灣之養殖與產銷…………………………….…………….… 4
二、吳郭魚常見疾病及防治………………………………………………….… 4
(一) 水環境引起疾病……………………………….………………….. 12
(二) 寄生蟲疾病……………………………………….…………….…. 12
(三) 細菌性疾病………………………………………………………... 13
三、孔雀綠的化學結構與理化性狀………………………………………….….. 15
四、孔雀綠在水產養殖上的應用與毒性…………………………………….. 17
(一) 孔雀綠在水產養殖上的應用………..………………………….…
17
(二) 孔雀綠的毒性………………………….…………………….….… 19
五、孔雀綠之殘留性………………………………………………………….. 20
(一) 孔雀綠在魚體之代謝與殘留……………………………………... 20
(二) 影響孔雀綠在水中濃度變化及魚體半致死濃度之因子………... 22
(三) 不同樣品處理條件對孔雀綠殘留量之影響……………………… 23
六、消除水中孔雀綠的方法………………………………………………….. 25
(一) 活性碳吸附法…………………………………………………….. 26
(二) 化學修飾法………………………………………………………… 27
(三) 微生物處理法………………………………………………………. 28
七、孔雀綠和還原型孔雀綠分析方法之探討…………………………….……… 29
(一) 樣品處理方法…………………………………………………….. 29
(二) 儀器分析方法………………………………………………….… 33
肆、試驗材料與方法…………………………………………………………….. 41
一、試驗材料…………………………………………………………………..
41
(一) 試驗動物…………………………………………………………. 41
(二) 試藥…………………………………………………………….… 41
二、試驗方法 42
(一) 藥浴不同孔雀綠濃度對魚體殘留性之影響………………….… 42
(二) 不同魚隻密度藥浴孔雀綠對魚體殘留性之影響……………….
42
(三) 不同水環境藥浴孔雀綠對魚體殘留性之影響……………….… 43
(四) 孔雀綠與還原型孔雀綠之分析……………………………….… 44
(五) 資料統計分析……………………………………………………. 48
伍、結果與討論………………………………………………………………….. 49
一、藥浴濃度對魚體中孔雀綠之變化……………………………………….… 49
(一) 藥浴期間魚體中孔雀綠及還原型孔雀綠之變化……………….. 49
(二) 藥浴後於換水蓄養期間魚體中孔雀綠及還原型孔雀綠之變化 51
二、魚隻密度對魚體中孔雀綠之變化……………………………………….. 61
(一) 藥浴期間魚體中孔雀綠及還原型孔雀綠之變化…………….… 61
(二) 藥浴後於換水蓄養期間魚體中孔雀綠及還原型孔雀綠之變化 62
三、水環境對魚體中孔雀綠之變化…………………………………………..
70
(一) 藥浴期間魚體中孔雀綠及還原型孔雀綠之變化…………………. 70
(二) 藥浴後於換水蓄養期間魚體中孔雀綠及還原型孔雀綠之變化 71
陸、結論………………………………………………………………………….. 79
柒、參考文獻…………………………………………………………………….… 81
捌、英文摘要………………………………………………………………….…… 90
王明光、王敏昭 (2003) 實用儀器分析。合記圖書出版社。台北。
王進崑、柯文慶、洪端良、陳重文、盧榮錦、賴滋渶 (2002)。食品營養儀器分析。富林出版社。台中。
方嘉佑 (2002) 藥品分析。合記圖書出版社。台北。
台灣鯛協會 (2005) 認識台灣鯛(參)台灣鯛的演進。2005年4月21日下載自http://www.taiwantilapia.org/chinese/t2a01_03.html
行政院農業委員會 (2004) 93年3月份農委會重要措施。2005年3月21日下載自http://www.coa.gov.tw/view.php?catid=6326
行政院農業委員會漁業署漁業資訊服務網 (2004-2005) 養殖水產品衛生品質監視檢驗結果。2007年3月27日下載自http://www.fa.gov.tw/chn/organization/monitor/monitor.php。
行政院農業委員會漁業署 (1971-2005) 中華民國台閩地區漁業統計年報。台北。
李武忠、陳郁蕙、廖一久 (2004) 吳郭魚養殖產業發展策略之研究(1)。養魚世界,325:15-21。
李茂榮 (1995) 液相層析質譜儀介面技術及應用。Chemistry,53(4):411-423。
李國誥 (2004) 水產養殖之藥物防治。養殖漁業經營管理手冊技術篇。2005年10月21日下載自http://www.fa.gov.tw/fshadm/aqfommt/aqchap03.htm
凌永健 (1997) 質譜儀。科儀新知,19(2):31-39。
涂堅 (1992) 水產動物疾病防治-吳郭魚常見疾病及防治(一)細菌性疾病。2006年4月21日下載自http://www.nvri.gov.tw/veter-info/index.htm。
陳仁焜 (2001) 液相層析/質譜/質譜的原理與其在食品科學領域的應用。科學與技術,33(3):44-54。
陳正和 (2003) 由原吳郭魚易名經濟價值大幅提昇的「臺灣鯛」。養魚世界,316:44-47。
陳平發 (2001) 吳郭魚為二十一世紀之魚。養魚世界,293:65-67。
郭河 (1993) 吳郭魚養殖,魚病專輯4-虱目魚、吳郭魚。台灣養豬科學研究所。台灣。
黃世鈴 (1985) 魚病診斷與防治。漁業推廣專輯(三)。台灣省漁業局。台北。
黃寶慧、李茂榮 (1998) 固相萃取技術。Chemistry,56(4):319-326。
黃鵬鵬 (1988) 淡水魚能活在海水裏嗎?—真骨魚滲透壓調節機制之研究。科學月刊,225:668-675。
湯有光、徐財生 (2003) 肉品中殘留有機氯劑農藥之檢驗—液、固相萃取法之比較研究。標準與檢驗,59:58-84。
詹舜安、劉宗榮 (2002) 液相層析串聯式質譜儀之原理及應用。科儀新知,24(1):77-89。
蔡添財 (2005) 養殖漁業-吳郭魚,台灣農家要覽漁業篇,增修訂三版。行政院農業委員會。台北。
行政院農業委員會藥物毒物試驗所 (2000) 農藥檢驗技術標準與鑑定。農毒所專題報導,57:1-10。
Alderman, D. J. (1985). Malachite green: a review. J. Fish Dis. 8:289-298.
Alderman, D. J. & Clifton-Hadley, R. S. (1993) Malachite green : a pharmacokinetic study in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Fish Dis., 16:297-311.
Allen, J. L., Gofus, J. E. & Meinertz, J.R. (1994). Determination of malachite green residues in the eggs, fry, and adult muscle tissue of rainbow trout (Oncorhynchus mykiss). J. A.O.A.C. Inter., 77:553-557.
Bauer, K., Dangschat, H., Knoppler, H. O. & Neudegger, J. (1988). Uptake and excretion of malachite green in rainbow trout. Arch .Lebensm. Hyg., 39:97-102. (English abstract)
Bergwerff, A. A., Kuiper, R. V. & Scherpenisse, P. (2004). Persistence of residues of malachite green in juvenile eels (Anguilla anguilla). Aquaculture, 233:55-63.
Bergwerff, A. A. & Scherpenisse, P. (2003). Determination of residues of malachite green in aquatic animals. J. Chromatogr. B, 788:351–359.
Campbell, R. E., Lilley, J. H., Panywachira, V. & Kanchanakhan, S. (2001). In vitro screening of novel treatments for Aphanomyces invadans. Aquacult. Res., 32:223-233.
Clemmensen, S., Jensen, J. C., Jensen, N. J., Meyer, O., Olsen, P., & Wurtzen, G. (1984). Toxicological studies of malachite green : a triphenyl methane dye. Arch. Toxicol., 56:43-45.
Culp, S. J. & Beland, F. A. (1996). Malachite green: a toxicological review. J. Am. Coll. Toxicol., 15:219-238.
Culp, S. J., Blankenship, L. R., Kusewitt, D. F., Doerge, D. R., Mμlligan, L. T. & Beland, F.A. (1999). Toxicity and metabolism of malachite green and leucomalachite green during short-term feeding to Fischer 344 rats and B6C3F1 mice. Chem. Biol. Interact., 122:153-170.
Culp, S. J., Beland, F. A., Heflich, R. H., Benson, R. W., Blankenship, L. R. , Webb, P. J., Mellick, P. W., Trotter, R. W., Shelton, S. D., Greenlees, K. J. & Manjanatha, M. G.. (2002). Mutagenicity and carcinogenicity in relation to DNA adduct formation in rats fed leucomalachite green. Mutat. Res., 506–507:55–63.
Doerge, D. R., Chang, H. C., Divi, R. L. & Vhurchewell, M. I. (1998). Mechanism for inhibition of thyroid peroxidase by leucomalahchite green. Chem. Res. Toxicol., 11:1098-1104.
Desciens, R. & Bablet, J. (1994). Recherches sur la toxicite des derives triphenylmethaniques anthelminthiques on the laminthgues. Comp. Rendus. Soc. Biol., 138:838-839.
European Commission, Commission Decision 657/2002/EC (2002). Implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Off. J. Eur. Commun. L 221/8.
European Commission, Commission Decision 25/2004/EC (2004). Amending Decision 2002/657/EC as regards the setting of minimum required performance limits (MRPLS) for certain residues in food of animal origin. Off. J. Eur. Commun. L 006/38.
Fessard, V., Godard, T., Huet, S., Mourot, A., & Poμl, J. M. (1999). Mutagenicity of malachite green and leucomalachite green in vitro tests. J. Appl. Toxicol., 19:421-430.
Garg, V. K., Gupta, R., Yadav, A. B. & Kumar, R. (2003). Dye removal from aqueous solution by adsorption on treated sawdust. Bioresource Technol., 89:121–124.
Gerundo, N., Alderman, D. J., Clifton-hadley, R. S. & Feist, S. W. (1991). Pathological effects of reqeated dosed of malachite green: preliminary study. J. Fish Dis., 14:521-532.
Gong, R., Jin, Y., Chen, F., Chenb, J.& Liu, Z. (2006). Enhanced malachite green removal from aqueous solution by citric acid modified rice straw. J. Hazard. Mater. B, 137:865-870.
Guo, Y., Yang, S., Fu, W., Qi, J., Li, R., Wang, Z. & Xu, H.(2003). Adsorption of malachite green on micro- and mesoporous rice husk-based active carbon. Dyes and Pigments, 56:219–229.
Keyl, H. G. & Werth, G. (1959). Stoukfurveranderungan chromosomen durch malachitgrun. Naturwissenschaften, 46:453-454. (English abstract)
Ma´chova´, J., Svobodova´, Z., Svobodnı´k, J., Piacka, V., Vykusova´, B., & Kocova´, A. (1996). Persistence of malachite green in tissues of rainbow trout after a long-term therapeutic bath. Acta Vet. Brno, 65:151–159. (English abstract)
Meyer, F. P. & Jorgensen, T. A. (1983). Teratological and other effects of malachite green on the development of rainbow trout and rabbits. Trans. Am. Fish. Soc., 112:818-824.
Mitrowska, K. & Posyniak, A. (2004). Determination of malachite green and its metabolite, leucomalachite green, in fish muscle by liquid chromatography. Bull. Vet. Inst. Pulawy, 48:173-176.
Mitrowska, K., Posyniak, A. & Zmudzki, J. (2005). Determination of malachite green and leucomalachite green in carp muscle by liquid chromatography with visible and fluorescence detection. J. Chromatogr. A, 1089:187-192.
Papinutti, L. & Forchiassin, F. (2004). Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete Chrysosporium. FEMS Microbiol Lett., 231 : 205-9.
Papinutti, L., Mouso, N. & Forchiassin, F. (2006). Removal and degradation of the fungicide dye malachite green from aqueous solution using the system wheat bran–Fomes sclerodermeus. Enzyme and Microbial Technol., 39:848-853.
Plakas, S. M., El Said, K. R., Stehly, G. R., Gingerich, W. H. & Allen, J. L. (1996). Uptake, tissue distribution, and metabolism of malachite green in the channel catfish (Ictalurus punctatus). Can. J. Fish. Aquat. Sci., 53:1427-1433.
Pointing SB. (2001). Feasibility of bioremediation by white-rot fungi. Appl. Microbiol. Biotechnol., 30:157-60.
Rahman, I. A., Saad, B., Shaidan, S. & Rizal, S. E. S. (2005). Adsorption characteristics of malachite green on activated carbon derived from rice husks produced by chemical–thermal process. Bioresour. Technol., 96:1578-1583.
Rajeshwarisivaraj, V. S. (2002). Activated parthenium carbon as an adsorbent for the removal of dyes and heavy metal ions from aqueous solution. Bioresour. Technol., 85:205-206.
Scherpenisse, P. & Bergwerff, A. A. (2005). Determination of residues of malachite green in finfish by liquid chromatography tandem mass spectrometry. Anal. Chim. Acta, 529:173-177.
Srivastava, S., Sinha, R. & Roy, D. (2004). Toxicological effects of malachite green. Aquat. Toxicol., 66: 319-329.
Turnipseed, S. B., Anderson, W. C. & Roybal, J. E. (2005) Determination and confirmation of malachite green and leucomalachite green residues in salmon using liquid chromatography/mass spectrometry with no-discharge atmospheric pressure chemical ionization. J. AOAC Int., 88:1312-1317.
Werth, G. (1958). Die erzeμgung von storungen im erbgefμge und von tumoren durch experimentelle gewebsanoxie. Arzn. Forsch., 8:725-744. (English abstract)
Werth, G. & Boiteaux, A. (1967). The toxicology of the triphenylmethane dyestuff, malachite green, as an uncoupler of oxidative phosphorylation in vivo and in vitro. Archives fur Toxicologie 23, 82-103. (English abstract)
Wing, R. E. (1996). Corn fiber citrate: preparation and ion exchange properties. Ind. Crop Prod., 5:301–305.
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