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研究生:林穎婷
研究生(外文):Ying Ting Lin
論文名稱:半邊蓮之化學成分與生物活性研究
論文名稱(外文):Chemical and Biological Investigations of Lobelia chinensis Lour.
指導教授:呂彥禮
指導教授(外文):Y. L. Leu
學位類別:碩士
校院名稱:長庚大學
系所名稱:天然藥物研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
論文頁數:113
中文關鍵詞:半邊蓮
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半邊蓮 (Lobelia chinensis Lour. )。屬於桔梗科 (Campanulaceae),山梗菜屬(Lobelia) 植物,為多年生草本植物,主要分布於台灣、中國。
半邊蓮甲醇粗抽物經藥效篩選後,在抗第一型單純疱疹病毒具有顯著的效果。於抗發炎藥理測試中得知半邊蓮氯仿可溶層,對以fMLP/CB刺激人類嗜中性白血球所引起超氧自由基釋放與elastase釋放有抑制作用,其IC50值皆低於10 μg/ml。於抗肺癌A549細胞株的測試中,發現氯仿可溶層具有43.57%抑制肺癌細胞株A549生長的作用。因此對半邊蓮之化學成份與生物活性作詳細的研究。
乾燥半邊蓮全株(1985 g)以甲醇浸泡冷、熱浸萃取,所得濾液經減壓濃縮後,分別以水、氯仿和正丁醇進行多次分配萃取,各分配層以管柱分離純化,總共分離52個化合物,其中45個化合物經光譜分析、文獻比對,確認其化學結構,其中以黃酮類化合物為主要成份。Lobechine為天然界首次分離的化合物。再將所分離得的化合物進行藥理試驗,在抗發炎的測試中發現scoparone具有抑制超氧自由基釋放的活性。
Lobelia chinensis Lour. is one species of Lobelia plant (Campanulaceae) in Taiwan and China.
The results of anti-HSV-1 experiment indicated that the crude extracts of L. chinensis Lour., significantly inhibited HSV-1 replication. The chloroform soluble layer inhibited the release of superoxide anion and elastase in fMLP/CB-activated human neutrophils, and the IC50 values were lower than 10 μg/ml. The chloroform layer also inhibited 43.57% the growth of lung carcinoma cell line A549 cell.
The dry whole plants (1985 g) of L. chinensis Lour. were extracted by methanol, filtered and then concentracted under reducing pressure to give dark brown extracts. The crude extracts were partitioned by water and chloroform, and n-BuOH, separately. Each layer was chromatographed by column chromatography, thin layer chromatography and recrystallizd to afford 52 compounds. Among them 45 compounds were determined their structures by spectral analysis and compared with authentic literatures. The major components were flavonoids. Lobechine was isolated for the first time from the natural source. In the anti-inflammatory experiments, scoparone showed the inhibition on inflammatory responses.
誌謝.....iv
目錄.....v
中文摘要......vii
英文摘要......viii
第一章........1
第二章........3
第三章.......13
第四章.......28
第五章.......64
第六章.......70
第七章.......72
光譜數據整理.....76
參考文獻.........96
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