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研究生:邱秉毅
研究生(外文):Biing-Yih Chiou
論文名稱:聚甘露糖�‘芠ㄤ艉尿z選及培養條件探討與酵素特性分析
論文名稱(外文):Isolation and cultivation conditions of a mannanase producing microorganism and characterization of enzyme properties
指導教授:林澤群林澤群引用關係
指導教授(外文):Tse-Chun Lin
學位類別:碩士
校院名稱:中臺科技大學
系所名稱:食品科技研究所
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:104
中文關鍵詞:Aspergillus niger聚甘露醣葡萄糖甘露聚醣半乳糖甘露聚醣聚甘露糖
外文關鍵詞:mannanaseAsp. nigermannanglucomannansgalactomannans
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本研究以中部地區土壤篩選出一株可分泌高單位聚甘露糖�′〝吨孝蒏銵C經鏡檢觀察其特徵及比對其18S rDNA 序列後,暫命為 Aspergillus niger NCH-318。Asp. niger NCH-318 所生產之聚甘露糖�〃妡簿嶸S性如下:最適反應 pH 4.0,最適反應溫度為60℃,最適反應基質濃度為0.9%。Asp. niger NCH-318 較適培養基與培養條件如下:培養基為Mandels-Reese medium(M),碳源為去油椰子粕,添加量為1.5%,氮源為酵母萃取物,添加量為0.56%,培養溫度為40 ℃,起始 pH 為4.0,接種量為106 spores/ml,震盪速率為110 rpm,在培養六天後,可得最高酵素活性,約為190 U/ml。
A high mannanase-producing fungal strain was isolated soil sample in central Taiwan. According to morphology and 18S rDNA comparison by microscope and comparison between the partial sequence of 18S rDNA, it was identified to be Asp. niger and was therefore named temporarily as A.niger NCH-318. The enzyme properties of crude mannanase are described as follows: the optimum pH was pH 4.0 and optimum temperature was 60℃. The optimum substrate concentration was 0.9%. The optimal medium composition and cultivation conditions for A. niger NCH-318 are determined using shaking flasks (capacity 250 ml). The results showed that Mandels-Reese medium (M) with 1.5% coconut meal which was defatted by n-hexane, 0.56% yeast extract as nitrogen source, incubation temperature at 40℃, initial pH at 4.0, inoculum size at 106 sproes/ml and shaking rate at 110 rpm. The final enzme activity of 190 U/ml in the culture broth was obtained after 6 days of cultivation.
表目錄 iii
圖目錄 iv
中文摘要 1
Abstract 2
壹、前言 3
貳、文獻回顧 5
一、聚甘露糖之分佈 5
二、植物中之聚甘露糖 5
三、聚甘露糖�﹞孝硎c種類 11
四、聚甘露糖�﹞夾虓� 14
五、聚甘露糖�﹞尾野� 14
參、實驗材料及方法 18
一、實驗材料: 18
二、儀器設備 23
三、套裝軟體 24
四、實驗流程 25
五、實驗方法: 26
肆、結果與討論 36
(一)、聚甘露糖�‘芠ㄤ艉坐擢鷋P篩選 36
(二)、菌種鑑定 39
(三)、聚甘露糖�‘芠ㄤ蒏頦�適基礎培養基之探討 47
(四)、聚甘露糖�〃妡簿徽G性質之分析 52
(五)、聚甘露糖�‘芠ㄤ蒏頦�適培養基及培養條件之探討 59
伍、結論 88
參考文獻 89
附錄一、Asp. niger NCH-318之DNA 定序圖譜 104
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