臺灣博碩士論文加值系統

魚針草（Anisomeles indica O. Kuntze），為唇形科（Labiatae）之一年或越年生草本植物，全草有解熱、袪風、除濕、健胃、解毒、止痛、抗菌之功效。一般民間常用於治療風濕性關節炎、腸胃炎及其它炎症等。本研究針對魚針草莖部之水萃取物部份，進行分離純化、化學結構鑑定、抗發炎藥理活性評估及有效成分定量等。在分離純化方面，以熱水萃取魚針草，再將熱水萃取物以極性分配為氯仿層極正丁醇層，各層再以矽膠管柱層析、薄層層析、化合物再結晶等進行分離純化，實驗結果得到9個化合物，其中4個化合物經各種光譜分析，結構鑑定證實為β-sitosterol、stigmasterol、acteoside、ovatodiolide等。而已單離化合物之抗發炎生物活性測試及其它結構的鑑定上在進行中。而在有效成分定量方面，針對魚針草內酯 (ovatodiolide)之提取，探討最佳萃取效率與 HPLC定量分析研究。實驗結果，於熱水萃取之粗萃取物中添加正己烷為極性分配之溶劑時，能快速且有效率地得到魚針草內酯，經反覆試驗之後，初估以每1公斤的魚針草莖部得水萃取之粗萃取物為253.17 g，可得到約10.6 mg的晶體，產量為238.84 ppm。其化合物純度可高達95 %以上。

Anisomeles indica O. Kuntze (Labiatae) has been used for treating damp heat, carminative, stomachic, detoxification, pain relief, rheumatoid arthritis, gastroenteritis and other inflammatory diseases in folk medicine. In present study, the stem parts of A. indica were extracted with hot water. The combined water extract was partition with chloroform and n-butanol. Each layer was concentrated under reduced pressure individually, and then subjected to silica gel column chromatography, thin-layer chromatography, and recrystallization. Nine compounds were isolated among 4 compounds structures were also determined. The four compounds namely : β-sitosterol, stigmasterol, acteoside, ovatodiolide. Now studies are under progress on these isolates for their anti-inflammatory properties. In effective component quantification aspect, in view of ovatodiolide, discuss the best way to extraction efficiency and quantitative analysis by HPLC. The experimental result pointed out the combined water extract was partition with n-hexane can fast and effectiveness to purify ovatodiolide from A. indica. After repeatedly experiments, the study estimate that one kilogram stems part of A. indica can get crude extract 253.17 g, and 10.6 mg of ovatodiolide. The yield is 238.84 ppm and purity over 95%.

書頁名
博、碩士論文授權書
論文口試委員會審定書
中文摘要..................................................................................................I
英文摘要.................................................................................................II
誌謝......................................................................................................III
目錄..................................................................................................... IV
表目錄..................................................................................................VII
圖目錄................................................................................................VIII
第一章、 緒論..........................................................................................3
第二章、 文獻回顧...................................................................................5
2-1 魚針草簡介.......................................................................................5
2-2 魚針草植物學分類.............................................................................6
2-3 魚針草植物型態特徵.........................................................................6
2-4 魚針草民間傳統方例.........................................................................9
2-5 魚針草文獻回顧................................................................................9
2-6 魚針草化學成分回顧.......................................................................10
2-7 魚針草之藥理回顧...........................................................................12
2-8 發炎反應.........................................................................................17
2-9 發炎調節因子..................................................................................19
2-10 初級發炎調節因子.........................................................................20
2-10-1 腫瘤壞死因子.............................................................................20
2-10-2 IL-1.............................................................................................22
2-10-3 IL-6.............................................................................................22
2-10-4 IL-12...........................................................................................23
2-11 一氧化氮.......................................................................................24
2-12 中草藥對iNOS基因表達的影響.....................................................25
第三章、 魚針草成份萃取與分離純化.....................................................27
3-1 前言................................................................................................27
3-2 研究材料與方法..............................................................................28
3-2-1 實驗材料......................................................................................28
3-2-2 實驗設備......................................................................................28
3-2-3 實驗方法......................................................................................29
3-3 結果與討論.....................................................................................30
3-4 氯仿層分離化合物...........................................................................32
3-5 正丁醇層分離化合物.......................................................................33
第四章、 魚針草內酯結構鑑定及有效成份製備與分析.............................38
4-1 前言................................................................................................38
4-2 材料與方法.....................................................................................39
4-2-1 實驗材料與設備...........................................................................39
4-2-2 實驗方法......................................................................................40
4-4魚針草內酯有效成份製備與分析.......................................................46
4-4-1 簡介.............................................................................................46
4-4-2-1實驗材料....................................................................................47
4-4-2-1實驗設備....................................................................................47
4-4-3實驗方法.......................................................................................48
4-5 結果與討論.....................................................................................50
第五章、結論與展望..............................................................................54
參考文獻...............................................................................................56
附錄......................................................................................................69
附錄I 魚針草化合物之藥理活性測試......................................................69
附錄II魚針草水萃取物對解苷酶(α- glucosidase)之活性測試...................70
作者簡歷...............................................................................................72





表目錄
表3-1、熱水萃取各層分離化合物產物重及含量表………………………….36
表4-1、ovatodiolide 之氫譜與碳譜數據整理表……………………………...42
































圖目錄
圖1-1、實驗設計流程圖............................................................................4
圖2-1、魚針草外觀型態圖.......................................................................8
圖2-2苯乙醇苷類化合物........................................................................15
圖2-3固醇類化合物...............................................................................15
圖2-4 ovatodiolide 及 isoovatodiolide......................................................16
圖2-5 黃酮類化合物..............................................................................16
圖3-1、魚針草莖部熱水萃取物分離流程圖.............................................31
圖3-2、分離化合物之產量含量對照圖.....................................................37
圖4-1、魚針草內酯 (ovatodiolide) 之1H-NMR圖譜..................................43
圖4-2、魚針草內酯 (ovatodiolide) 之13C-NMR圖譜.................................44
圖4-3、魚針草內酯 （ovatodiolide） 之ESI-Mass圖譜................................45
圖4-4、魚針草內酯萃取純化流程圖........................................................49
圖4-5、分析型HPLC對魚針草內酯之分析圖譜.......................................51
圖4-6、分析型HPLC對魚針草內酯之分析圖譜.......................................52
圖4-7、魚針草內酯(ovatodiolide)之標準曲線圖....................................53

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