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研究生:林鈺傑
研究生(外文):Yu-Chien Lin
論文名稱:紫色不含硫光合菌結合不同生物系統產生氫氣之研究
論文名稱(外文):Hydrogen production by purple nonsulfur bacteria in combination with different biological system.
指導教授:李季眉李季眉引用關係
指導教授(外文):Chi-Mei Lee
學位類別:碩士
校院名稱:國立中興大學
系所名稱:環境工程學系所
學門:工程學門
學類:環境工程學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:99
中文關鍵詞:紫色不含硫光合菌藍綠菌
外文關鍵詞:purple nonsulfer bacteriacyanobacteria
相關次數:
  • 被引用被引用:6
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現今地球暖化的惡化,與石化燃料的耗竭嚴重影響人類的生活。而氫氣是能有效改善環境問題的能源之一,如能大量生產氫氣,將會對環境有正面的幫助,現階段氫氣生產的方法包括石化燃料產生氫、水分解產生氫氣與生物方等。
本研究欲利用生物方法產生氫氣,現今具有產生氫能力的微生物有綠藻、藍綠菌、紫色光合作用細菌與厭氧菌。由於藍綠菌、紫色光合作用細菌與厭氧菌各有不同的產氫條件,本研究分為三個部份探討。
第一部分為高溫好氧污泥消化連續流系統提升有機酸產生的試驗,操作策略分為再次添加高溫菌、修改水力停留時間與提升進流污泥MLSS濃度。其中,修改水力停留時間與提升進流污泥的MLSS濃度都能提升有機酸的生成,平均有機酸產量為1072 mg/L;隨著有機酸的提升,抑制紫色光合作用細菌產氫的NH4+-N濃度也亦增加274 mg/L,將出流水進行紫色光合作用細菌產氫試驗,結果顯示紫色不含硫光合菌biomass產量提升,但氫氣生成被NH4+所抑制,故以高溫好氧消化污泥出流水不適合紫色光合細菌產氫。
第二部分為由台中某生活污水廠污泥經由熱篩程序,分離出厭氧混合族群,藉由Endo培養基測試其是否具有產氫能力,所分離菌群具有產氫能力,並再以廚餘進行厭氧發酵菌產氫批次試驗並將其所得的液態產物作為光合細菌批次產氫試驗,作為兩階段生物產氫。結果顯示,廚餘作為厭氧產氫菌與光合細菌串聯產氫是適合的基質。第三部份則為藍綠菌與光合菌共培養初步建立適合兩種微生物產氫的光照系統。
Today global energy requirement are mostly dependent on fossil fuels. This will eventually lead to the foreseeable depletion of limited fossil energy resources. Presently, the utilization of fossil fuels is causing global climate change. In order to remedy the depletion of fossil fuels and their environmental misdeeds, hydrogen has been suggested as the energy carrier of the future.
It is known that hydrogen may be produced by fermentative bacteria and phototrophs of different tamxonomic groups including several species of purple nonsulfur bacteria and cyanobacteria. If the VFAs, produced in anaerobic process by fermentative bacteria, are used as substrate for cocultivation cells of cyanobacteria and purple nonsulfer bacteria to produce hydrogen, the hydrogen yield will be increased.
In order to increase the hydrogen production by Rhodopseudomonas palustris WP3-5 using the pretreated effluent of thermophilic aerobic digestion (TAD), the first part of this research was to increase the organic acids content of the effluent of continuous TAD reactor. Strategies included the addition of thermophilic bacteria, extending the hydraulic retention time as well as increasing MLSS concentration of influent sludge. The result indicated that extending the hydraulic retention time and increasing MLSS concentration increased the organic acid production. The average organic acid concentration was 1072 mg/L. However, the NH4+-N concentration, which inhibited the photohydrogen production, raised to 274 mg/L with increasing organic acid concentrations. If this pretreated effluent of TAD was used for photohydrogen production, it can be seen that hydrogen production was inhibited by NH4+-N while biomass increased. Therefore the effluent of TAD is not suitable for photohydrogen production.
The second part of this research was to investigate the anaerobic hydrogen production process by photosynthetic hydrogen production process. The result indicated that kitchen waste was suitable for this purpose.
The third part of this research was to cocultivate cyanobacteria and photosynthetic bacteria, the illumination system for the cultivation was wstablished.
摘要 I
英文摘要 II
目錄 IV
圖目錄 VII
表目錄 X
第一章 前言 1
第二章 文獻回顧 3
2-1 現今環境與能源問題 3
2-2 再生能源的開發 4
2-3 生物方法產氫 5
2-3-1 厭氧發酵產氫 7
2-3-1-1 厭氧發酵產氫之微生物 9
2-3-1-2 厭氧發酵產氫代謝機制 9
2-3-1-3 影響厭氧發酵產氫之因素 12
2-3-2 紫色不含硫光合菌產氫 14
2-3-2-1 紫色不含硫光合菌光合代謝與產氫機制 15
2-3-2-2 影響紫色不含硫光合菌產氫之因素 16
2-3-3 藍綠菌產氫 19
2-3-3-1 藍綠菌光合代謝與產氫機制 19
2-3-3-2 影響藍綠菌光合產氫之因素 22
2-3-4 產氫微生物結合之研究 24
2-4 生物產氫之基質來源 26
2-4-1 活性污泥消化再利用 28
2-4-2 高溫好氧消化污泥代謝機制 29
2-4-3高溫好氧消化污泥累積有機酸之研究 31
第三章 材料與方法 33
3-1 實驗架構 33
3-2 菌種來源 35
3-2-1 好氧高溫菌來源 35
3-2-2 紫色不含硫光合菌來源 35
3-2-3 厭氧發酵菌來源 35
3-2-4 藍綠菌來源 35
3-3 菌種保存與培養 35
3-3-1 好氧高溫菌 35
3-3-2 紫色不含硫光合菌 36
3-3-3 厭氧發酵菌 38
3-3-4 藍綠菌 39
3-3-5 藍綠菌與紫色不含硫光合菌共培養 40
3-4實驗方法 41
3-4-1 高溫好氧污泥消化連續流試驗 41
3-4-2 厭氧發酵產氫批次試驗 41
3-4-3 紫色不含硫光合菌利用發酵出流水產氫批次試驗 43
3-4-4 紫色不含硫光合菌利用藍綠菌照光系統產氫批次試驗 44
3-4-5 紫色不含硫光合菌利用果糖產氫批次試驗 44
3-4-6 紫色不含硫光合菌與藍綠菌共培養產氫批次試驗 45
3-5 分析設備與方法 45
3-6 實驗用水與鹼洗液 49
第四章 結果與討論 51
4-1 高溫好氧消化污泥產酸提供紫色不含硫光合菌產氫試驗 51
4-1-1 連續流初始操作條件測試 51
4-1-2 再次添加菌株S2測試 54
4-1-3 提升水力停留時間試驗 56
4-1-4 增加進流污泥MLSS濃度 58
4-1-5 初步結論 61
4-2 不同基質來源進行厭氧發酵俊與紫色不含硫光合菌串連批次試驗 62
4-2-1 Endo培養基試驗 63
4-2-2 廚餘(含固體物廚餘)試驗 66
4-2-3 溶解性廚餘(不含固體物廚餘)試驗 71
4-2-4 酒糟廢水試驗 77
4-2-5 初步結論 81
4-3 藍綠菌與光合菌共培養產氫 81
4-3-1 紫色不含硫光合菌WP3-5在白光與黃光系統光合產氫比較 82
4-3-2 紫色不含硫光合菌WP3-5分別利用果糖與醋酸產氫試驗 84
4-3-3 藍綠菌CH3與紫色不含硫光合菌WP3-5以果糖與醋酸共培養試驗 86
4-3-4 初步結論 86
第五章 結論與建議 88
5-1 結論 88
5-2 建議 89
參考文獻 90
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