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研究生:林慧怡
研究生(外文):Hui-Yi Lin
論文名稱:台灣原生藥用植物(龍珠、台灣蒲公英、腎葉山螞蝗、羅氏鹽膚木)之增殖系統研究
論文名稱(外文):Establishment of the Clonal Propagation of Medicinal Plants in Taiwan (Tubocapsicum anomalum(Fr. & Sav.)Makino , Taraxacum formosanum Kitamura , Desmodium renifolium(L.)Schindler , Rhus javanica L. var. roxburghiana(DC.)Rehd.& Wilson. )
指導教授:廖松淵
學位類別:碩士
校院名稱:國立中興大學
系所名稱:生命科學院碩士在職專班
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:65
中文關鍵詞:台灣藥用植物龍珠台灣蒲公英腎葉山螞蝗羅氏鹽膚木
外文關鍵詞:Tubocapsicum anomalum(Fr. & Sav.)MakinoTaraxacum formosanum KitamuraDesmodium renifolium(L.)SchindlerRhus javanica L. var. roxburghiana(DC.)Rehd.& Wilson.
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本實驗目的是利用組織培養技術,建立龍珠、台灣蒲公英、腎葉山螞蝗和羅氏鹽膚木此四種藥用植物的增殖系統,過程包含建立無菌培植體、芽體誘導、發根及健化移植。試驗結果發現龍珠頂芽優勢強,在腋芽的增殖上,於BA 0.1 mg/L + NAA 0.01 mg/L之MS培養基,雖然可獲得最多芽數,但產生的腋芽數只有2.3,且高度較矮,不易切芽,建議以MS為增殖培養基;在發根方面,不管在MS、1/2 MS或是添加IBA,培養4週後,發根率均為100%。台灣蒲公英的腋芽增殖以BA 0.6 mg/L + NAA 0.06 mg/L 之MS培養基可產生大量芽體;台灣蒲公英以根做為外植體誘導不定芽,則以BA 0.4 mg/L + NAA 0.04 mg/L產生的芽體數最多,但是變異率太高,雖然不含生長調節劑之MS誘導產生不定芽數較少,但是芽體較為健康,故仍以MS為培養基較適合;台灣蒲公英在MS培養基中,粗根誘導不定芽數比細根多,但變異率兩者間無顯著差異;台灣蒲公英無論在MS、1/2 MS或是添加IBA,培養3週後,發根率均為100%。腎葉山螞蝗在1/3 MS的生長情況比MS、B5培養基健康,但腋芽頂端容易黃化,推測1/3 MS仍不是其最理想之培養基;在腋芽增殖上,以BA 0.2 mg/L + NAA 0.02 mg/L 之1/3 MS培養基可增殖最多芽體;發根率不管是否添加IBA,至8週發根情況都不理想。羅氏顏膚木在BA 0.6 mg/L + NAA 0.06 mg/L的腋芽增殖數量為最多,而1/2 WPM + IBA 0.2 mg/L的發根率最佳。
The aims of this study were to establish of the clonal propagation of four medicinal plants in Taiwan.These are Tubocapsicum anomalum(Fr. & Sav.)Makino , Taraxacum formosanum Kitamura , Desmodium renifolium(L.)Schindler and Rhus javanica L. var. roxburghiana(DC.)Rehd.& Wilson. In vitro micropropagation of Tubocapsicum anomalum(Fr. & Sav.)Makino was established to get maximum shoots multiplication rate by cultivating stem-node on Murashige and Skoog (MS) medium supplemented with BA 0.1 mg/L+ NAA 0.01 mg/L and rooting rate achieved 100% by cultivating on MS and 1/2MS medium or they supplemented with IBA 0.2 mg/L for 4 weeks. In vitro micropropagation of Taraxacum formosanum Kitamura was established to get maximum shoots multiplication rate by cultivating stem-node on MS medium supplemented with BA 0.6 mg/L+ NAA 0.06 mg/L and rooting rate achieved 100% by cultivating on MS and 1/2 MS medium or it supplemented with IBA 0.2 mg/L for 3 weeks. Cultivating on Root-cutting of Taraxacum formosanum Kitamura was established to get maximum shoots multiplication rate on MS medium supplemented with BA 0.4 mg/L+ NAA 0.04 mg/L , but this medium caused the obvious variance. Although it induce less buds on MS medium , the adventitious shoot is more health . MS medium is better than BA 0.4 mg/L+ NAA 0.04 mg/L on induced root-cutting to adventitious shoot. In vitro micropropagation of Desmodium renifolium(L.)Schindler was growing better in 1/3 MS than MS or B5 medium ,but 1/3 MS is not the best medium in cultivating . In vitro micropropagation of Desmodium renifolium(L.)Schindler was established to get maximum shoots multiplication rate by cultivating stem-node on 1/3 MS medium supplemented with BA 0.2 mg/L + NAA 0.02 mg/L .Rooting rate is not good in 1/3 MS media or adding IBA 0.2 mg/L or 0.4 mg/L . In vitro micropropagation of Rhus javanica L. var. roxburghiana(DC.)Rehd.& Wilson was established to get maximum shoots multiplication rate by cultivating stem-node on WPM medium supplemented with BA 0.6 mg/L + NAA 0.06 mg/L . Rooting rate were best on 1/2 WPM medium adding IBA 0.2mg/L .
摘要……………………………………………………… i
Abstract ………………………………………………… ii
目次……………………………………………………… iv
表目次…………………………………………………… v
圖目次…………………………………………………… vi
縮寫表…………………………………………………… vii
前言……………………………………………………… 1
前人研究………………………………………………… 2
材料與方法……………………………………………… 12
結果……………………………………………………… 15
討論……………………………………………………… 50
結論……………………………………………………… 55
參考文獻………………………………………………… 56
附錄……………………………………………………… 62
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