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研究生:黃佳盛
研究生(外文):Chia-Sheng Huang
論文名稱:臺灣地區馬纓丹屬植物花色變異之研究
論文名稱(外文):Studies on the flower color variation of Lantana in Taiwan
指導教授:蔡進來蔡進來引用關係
指導教授(外文):Jinn-Lai Tsai
學位類別:博士
校院名稱:國立中興大學
系所名稱:生命科學系所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:85
中文關鍵詞:馬纓丹屬花色花青素矢車菊色素芍藥色素
外文關鍵詞:Lantanafloral coloranthocyanincyanidinpeonidin
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馬纓丹原生於中南美洲,在臺灣已馴化成具侵略性的雜草。歷年來各學者對於臺灣地區已馴化的馬纓丹見解不同,本研究共收集了44個馬纓丹樣本,其中包括39個野生個體及5個園藝栽培種。利用TLC、HPLC定性分析、分光光度計及核磁共振儀,分析這些植物花冠內次級代謝物花青素的組成。結果得知除了白花馬纓丹無花青素之外,43個馬纓丹樣本中,共測得A1~A6等6種主要花青素,已確定A1~A5的結構分別為cyanidin 3-glucoside、cyanidin 3,5-diglucoside、cyanidin 3- malonylglucoside等3種矢車菊類型色素,以及 peonidin 3,5- diglucoside 、peonidin 3-malonylglucoside等2種芍藥類型色素。依該A1~A6各種花青素含量百分率的平均比例可以將其區分為第一型:A2:A4:A5 (5:45:48)、第二型:A2:A4:A6 (23:42:3 或40:18:4)、第三型:A1:A3:A4 (35:28:4)及第四型:A2:A4 (18:84)等4類群。其中園藝栽培種與馴化野生者之間有顯著差異,而野生個體花冠內的花青素較為多樣。研究結果可作為探討臺灣地區馬纓丹屬植物間親緣關係的重要依據。
Lantana (Verbenaceae) is a naturalized plant in Taiwan and considered as a weed because of its invasiveness. A total of 44 Lantana plants, including 39 wild plants and 5 horticultural plants, were collected in Taiwan. In order to study the relationship of those plants, co-HPLC examined each anthocyanin peak and content ratio in the corollas of mature flowers as chemical characters, along with 2 morphological characters. As a result of the HPLC analysis, 6 anthocyanin peaks were detected in those 43 Lantana plants, except white flower of Lantana nivea , which exhibite no anthocyanin content. The five of them were identified as 3- glucoside, 3, 5- diglucoside and 3- malonylglucoside of cyanidin, and 3, 5- diglucoside and 3-malonylglucoside of peonidin. The anthocyanin ratios in each taxon are different; they presented the 4 groups of significant ratios, classified from TypeⅠ, A2:A4:A5(5:45:48); TypeⅡ, A2:A4:A6(23:42:3 or 40:18:4); TypeⅢ, A1:A3:A4 (35:28:4) and Type Ⅳ, A2:A4 (18:84). Based on these results, we can distinguish wild species from the cultivars, and understanding how the wild species are becoming more diverse. It may provide valuable information to elucidate the interrelationship of Lantana plants in Taiwan and contribute to the systematic treatment of them.
目 次
表次…………………………………………………………….................Ⅱ
圖次…………………………………………………………….................Ⅲ
摘要…………………………………………………………….................Ⅴ
英文摘要………………………………………………………..…….......Ⅵ
前言………………………………………………………………………...1
前人研究…………………………………………………………………...4
材料與方法…………………………………………………………...…..21
結果…………………………………………………………………….....39
討論……………………………………………………………………….64
結論…………………………………………………………………….....75
引用文獻………………………………………………………………….77
附錄一…………………………………………………………………….87
附錄二…………………………………………………………………….88
附錄三…………………………………………………………………….91








表 次
表1. 三種馬纓丹屬植物全世界標本之採集地 ..….…………………..4
表2. 臺灣地區引進的馬纓丹屬植物…………………………………...8
表3. 花青素配醣基的取代作用類型………………………………….17
表4. 馬鞭草科植物已鑑定之色素……..……………………………...20
表5. 本研究之臺灣地區馬纓丹屬植物材料………………………….24
表6. 臺灣地區各種馬纓丹之基本性狀……………………………….39
表7. 臺灣地區馬纓丹屬植物之花色……………………………….…44
表8. 臺灣地區馬纓丹屬植物花青素色層與光譜特性……………….50
表9. 臺灣地區馬纓丹屬植物花青素之分布……………….…………58
表10. 臺灣地區馬纓丹屬植物花冠花青素之比例與形態特徵……….60
表11.臺灣地區馬纓丹屬植物學名之演變.…….……….……….……..66
表12. 栽培馬纓丹雜異體歷史的假說……….…………………………72









圖 次
圖1. 馴化於臺灣地區的三種馬纓丹(顏,2006) .…………………....7
圖2. 近緣4種類黃酮化合物之結構式.……………….……………..11
圖3. 常見6種主要花青素之結構式…………………………….…...16
圖4. 研究架構之流程…………………………………………………22
圖5. 引進臺灣地區的馬纓丹屬植物...…………....……………..…...28
圖6. 臺灣地區馬纓丹屬植物之採集地.……………………….……..30
圖7. 色差計之L、a、b色度圖………………………………………32
圖8. 英國皇家園藝學會色票. ………………………………………33
圖9. 花青素純化步驟之流程…………………………………………36
圖10. 臺灣地區馴化馬纓丹與國外資料之比較………………………42
圖11. 供馬纓丹比色分析用之花冠……………………………………43
圖12. 馬纓丹(H32)之RP-HPLC層析圖………………………............47
圖13. 橙紅馬纓丹(A)與蕙蘭(B)色素之HPLC比較…………….........48
圖14. 馬纓丹花青素A2, A4(Cy 3-glc, Cy 3-mglc)之結構式……........49
圖15. 馬纓丹花青素A4(Cy 3-mglc) 之1H NMR 核磁共振光譜.......51
圖16. 馬纓丹花青素A4(Cy 3-mglc) 之二維核磁共振光譜………...52
圖17. 馬纓丹花青素A1(Cy 3,5-diglc)之結構式……………………...53
圖18. 馬纓丹花青素A1之1 H NMR核磁共振光譜…………………54
圖19. 馬纓丹花青素A3(Pn 3,5-diglc)之結構式………………………55
圖20. 馬纓丹花青素A5(Pn 3-mglc)之結構式………………………...56
圖21. 馬纓丹形態與化學性狀之歸群分析…………………………...63
圖22. 園藝栽培種彩花馬纓丹之結實情形…………………………...68
圖23. 矮牽牛主要色素之生合成途徑………………………………...70
圖24. 花青素之生合成途徑……………………………………….......71
圖25. 臺灣地區馬纓丹花冠花青素之演化……………………….......74
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