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研究生:江冠威
研究生(外文):Guan-Wei Jiang
論文名稱:利用米水解液培養Lactobacilluskefiranofaciens之培養基組成及培養條件研究
論文名稱(外文):Study on medium composition and culture condition for the cultivation of Lactobacillus kefiranofaciens with rice hydrolysate
指導教授:陳錦樹陳錦樹引用關係
學位類別:碩士
校院名稱:國立中興大學
系所名稱:食品暨應用生物科技學系
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
畢業學年度:96
語文別:中文
論文頁數:117
中文關鍵詞:Lactobacillus kefiranofaciens胞外多醣水解米麴克弗爾
外文關鍵詞:Lactobacillus kefiranofaciensexopolysaccharidehydrolysisrice kojikefir
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  • 被引用被引用:3
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本研究以不同稻米產品(白米、糙米、紫米)為基質,利用米麴菌Aspergillus oryzae BCRC32279之澱粉酶及蛋白酶等酵素活性進行水解,所得米水解液,分別接種克弗爾乳酸菌Lactobacillus kefiranofaciens進行培養,其次探討其較適培養基組成及培養條件,並分析培養液中多醣等機能性成份產量之變化。結果顯示製麴之較適時間為2天,其澱粉酶活性約65.47 U/g,而酸性蛋白酶活性則約132.87 U/g。水解液之較適製備條件為米麴、添加米粉、水以1:1:6比例,於溫度55℃下水解10小時,可得到可溶性固形物含量約為14 oBrix之水解液。
米水解液稀釋4倍後添加1 % peptone、1 % beef extract及1 % yeast extract為氮源,再以鹽類金屬離子修飾,調整pH值之後可用於培養L. kefiranofaciens。添加1 % 乳糖補充碳源有助於多醣產量之提昇,經上述步驟調整後之培養基稱為修飾米水解液;L. kefiranofaciens於修飾米水解液中之較適培養條件為,起始pH 5.5於33℃下培養六天,可獲得最高多醣產量,約0.3 mg/mL,乳酸產量約30 mg/mL,而培養液中 GABA含量約0.1 mg/mL,不同米所製備之米水解液對於此等機能性成份產量並無明顯影響。另外, L. kefiranofaciens與Saccharomyces cerevisiae共同培養並無提昇多醣產量之效果。
Various kinds of rice products (polished rice, brown rice, and purple rice) were hydrolyzed by a rice koji Aspergillus oryzae BCRC32279, which has high enzymatic activities of α-amylase and protease, to obtain rice hydrolysates. The hydrolysates were then inoculated by a lactic acid bacterium Lactobacillus kefiranofaciens and cultivated. The optimal medium composition and cultivation conditions were studied and contents of some functional components including exopolysaccharide were studied during lactic acid fermentation. Results showed that the highest amylase activity of rice koji was 65.47 U/g and acid protease activity was 132.87 U/g after two days of cultivation. The optimum condition for preparing rice hydrolysate was rice koji, rice powder, and water in a ratio of 1:1:6, and the hydrolysis was performed at 55℃ for ten hours. The total soluble solid content of the rice hydrolysate was about 14 oBrix under this condition.
The rice hydrolysate was diluted 4-fold, and supplemented with 1 % peptone, 1 % beef extract and 1 % yeast extract as nitrogen source, and some mineral salts. Finally pH value was adjusted to 5.5. The medium was named as modified rice hydrolysate which can be used for cultivating L. kefiranofaciens. Addition of 1 % lactose enhanced further the production of exopolysaccharide. The highest exopolysaccharide production of L. kefiranofaciens with the modified rice hydrolysate was obtained at initial pH 5.5 and cultivated at 33℃ for 6 days. The content of exopolysaccharide was about 0.3 mg/mL, lactic acid was about 30 mg/mL, and γ-aminobutyric acid (GABA) content was about 0.1 mg/mL in the culture broth. Furthermore, the production or content of these functional components were not affected by the rice hydrolysates prepared by different rice products. Co-culture of L. kefiranofaciens with Saccharomyces cerevisiae didn’t promote exopolysaccharide production, either.
中文摘要 i
Abstract ii
目次 iii
表目次 iv
圖目次 v

壹、前言 1
貳、文獻回顧 2
一、稻米、糙米與紫米之介紹 2
二、米麴 6
三、克弗爾(kefir) 12
四、Lactobacillus kefiranofaciens 19
參、材料與方法 25
一、實驗材料 25
二、儀器設備 27
三、套裝軟體 28
四、實驗設計 28
五、實驗方法 29
六、分析方法 30
七、統計分析及圖形繪製 36
肆、結果與討論 37
一、水解液之製備 37
二、以米水解液培養 L. kefiranofaciens BCRC 16059之培養基組成探討 66
三、修飾米水解液培養 L. kefiranofaciens BCRC 16059之條件探討 81
四、機能性成份含量 102
伍、結論 108
一、水解液之製備 108
二、以米水解液培養 L. kefiranofaciens BCRC16059之培養基組成 108
三、修飾米水解液培養 L. kefiranofaciens BCRC16059之條件 108
四、機能性成份含量 108
陸、參考文獻 109
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