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研究生:劉恩妤
研究生(外文):En-U Liu
論文名稱:酸雨對菲律賓樟樹抗氧化系統的影響
論文名稱(外文):Effects of acid rain on antioxidative system In Cinnamomum philippinense seedlings
指導教授:劉瓊霦劉瓊霦引用關係
學位類別:碩士
校院名稱:國立中興大學
系所名稱:森林學系所
學門:農業科學學門
學類:林業學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:58
中文關鍵詞:酸雨氧化傷害抗氧化酵素
外文關鍵詞:acid rainoxidative damageantioxidative enzyme
相關次數:
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本試驗藉由探討菲律賓樟樹(Cinnamomum philippinense)在酸雨逆境下的抗氧化表現,以瞭解植物的抗氧化防禦作用在酸雨逆境的誘導反應。模擬酸雨是以SO42-及NO3-當量濃度比2.5:1的比例混合後,調整pH值為4、3及2,並以蒸餾水(pH 6)作為對照組。於酸雨噴灑1、10及15天後進行取樣分析。試驗結果為pH 4、3及2酸雨噴灑1天後,葉片內的H2O2含量立即高於對照組,進而誘發過氧化氫
The study was to examine the importance of the antioxidative defence mechanism during application of simulated acid rain pH 4, 3, 2 and pH 6 as control treatment in Cinnamomum philippinense seedlings. Simulated acid rain was a mixture of 2.5:1 equivalent concentration ratio of sulfuric and nitric acid and the pH value was adjusted with distilled water. Seedlings were sprayed with acid rain keeping on 15 days and sampled 1st, 10st, and 15st days during spraying acid rain period. Spraying acid rain 1 day , higher H2O2 induced catalase(CAT), ascorbate peroxidase(APx), dehydroascorbate reductase(DHAR), glutathione reductase(GR)and peroxidase(POD)antioxidative enzyme activity to relieve seedlings from oxidative stress. Therefore, Cinnamomum philippinense seedlings treated with acid rain of pH 4 and pH 3 for 10 days and pH 2 for 5 days still did not appear lipid peroxidation damage. However, after acid stress of pH 2 for 10 days, accumulated H+ content had already destoried CAT、APx、DHAR and GR antioxidative enzyme activities resulting in increasing reactive oxidative species content, lipid peroxidation damage, menbrance permeability and necrosis percentage. Although under acid stress of pH 2 for 15 days, Cinnamomum philippinense seedlings had lipid peroxidation seriously, POD activity and ascorbic acid ratio still increased significantly. This result represented POD and ascorbic acid contribute to the scavenging of reactive oxygen species in the oxidative burst situation, but plant still suffered from overmuch reactive oxidative species.
摘要……………………………………………………………………………………i
Abstract………………………………………………………………………………ii
目錄…………………………………………………………………………………iii
表目次………………………………………………………………………………vi
圖目次………………………………………………………………………………vii

一、前言…………………………………………………………………………………1
二、前人研究…………………………………………………………………………3
(一)酸雨對葉部外觀型態的影響………………………………………………3
(二)酸雨對細胞膜滲漏的影響…………………………………………………4
(三)酸雨對葉綠素及蛋白質的影響……………………………………………5
(四)酸雨對植株造成的氧化逆境………………………………………………6
(五)酸雨對植株造成的膜脂質過氧化傷害……………………………………8
(六)酸雨與植株內抗氧化系統的作用…………………………………………9
三、材料及方法………………………………………………………………………14
(一) 供試苗木…………………………………………………………………14
(二) 方法與步驟………………………………………………………………14
1酸雨的配置及處理方法……………………………………………………14
2取樣其分析步驟……………………………………………………………15
3植體分析方法………………………………………………………………15
(1) 葉片壞疽程度及細胞滲漏率分析……………………………………15
a 葉片的壞疽程度……………………………………………………15
b 細胞滲漏率…………………………………………………………16
(2) 葉綠素含量測定…………………………………………………………17
(3)可溶性蛋白質含量測定…………………………………………………17
(4)細胞的氧化逆境測定……………………………………………………18
a 過氧化氫含量測定…………………………………………………18
b 膜脂質過氧化物測定………………………………………………18
(5)抗氧化酵素活性測定……………………………………………………18
a 酵素的萃取方法……………………………………………………19
b 過氧化氫酶活性測定………………………………………………19
c 過氧化酶活性測定…………………………………………………20
d 抗壞血酸氧化酶活性測定…………………………………………20
e 抗壞血酸還原酶活性測定…………………………………………21
f 穀胱苷肽還原酶活性測定…………………………………………21
(6)抗壞血酸含量測定………………………………………………………22
a Total ascorbate22
b Ascorbate22
c Dehydroascorbate23
四、結果24
(一) 苗木的葉片病徵24
(二) 酸雨對細胞膜滲漏率的影響25
(三) 酸雨對蛋白質含量的影響………………………………………………25
(四) 酸雨對葉綠素含量的影響27
(五) 細胞的氧化逆境…………………………………………………………29
1 過氧化氫含量…………………………………………………………29
2 膜脂質過氧化物………………………………………………………29
(六) 抗氧化酵素活性…………………………………………………………31
1 POD及CAT活性………………………………………………………31
2 APx、DHAR 及GR活性………………………………………………33
(七) 膜脂質過氧化物相對含量與抗氧化酵素相對活性的相關性…………36
(八) 抗壞血酸含量……………………………………………………………37
五、討論………………………………………………………………………………39
(一) 酸雨對葉部外觀型態及細胞膜滲漏的影響……………………………39
(二) 酸雨對葉綠素及蛋白質含量的影響……………………………………40
(三) 酸雨對細胞造成的氧化傷害……………………………………………41
(四) 酸雨對抗氧化酵素活性的影響…………………………………………43
(五) 酸雨對抗氧化物的影響…………………………………………………47
六、結語………………………………………………………………………………48
七、引用文獻…………………………………………………………………………49
八、附錄……………………………………………………………………………… 58
束良佐、孫五三(2001)Ca2+ 浸種對酸雨傷害玉米幼苗的影響。中國環境科學 21(2): 185-188。
林曄理(1994)模擬酸雨與鹽霧對木麻黃苗木之危害。國立台灣大學森林學研究所碩士論文。
金恆鑣、劉瓊霦、夏禹九、黃正良(2003)福山天然闊葉林生態系對降水化學的交互作用。台灣林業科學 18(4): 367-373。
周青、黃曉華、王冬燕、宁允葉(1997)鈣對酸雨威迫下甜瓜幼苗質膜透性的影響。環境科學 18: 60-62。
周青、黃曉華、王丹、李雲(1999)鑭-甘氨酸稀土配合物對酸雨傷害植物的影響。應用與環境生物學報 5(6): 570-573。
周希琴(2005)木麻黃幼苗對模擬酸雨的迫害和Ca2+的調節作用。植物生理通訊 41(3): 3309-312。
邱棟樑、劉星輝、王湘平(2002)模擬酸雨對龍眼葉綠體的傷害效應。應用與環境生態學報 8(2): 154-158。
郭幸榮(1996)強化淨化污染樹種適應性之研究—酸性降雨適應性之研究行政院環保署綠化樹種篩選研究系列:「抗空氣污染之鄉土樹種及草種篩選」期末報告 8(1): 1-10。
陳澤裕(1984)西德地區之酸雨及其對森林的危害。中華林學季刊 17(2): 69-83。
張文東(2000)浸水逆境下玉米幼苗植株過氧化作用與抗氧化系統的反應。國立中興大學農藝系研究所博士論文。22-23頁。
黃日紅(1998)臭氧對水稻光合作用及抗氧化物之影響。國立中興大學植物學研究所碩士論文。8-9頁。
黃繼山、溫文寶、藺方煌、朱衛平、孔振蘭(2002)酸雨對樹木葉細胞傷害的模
擬研究。林業科學研究 15(2): 219-224。
葉超然(1993)酸性降水對木麻黃及樟樹生理之影響。國立台灣大學森林學研究所碩士論文。
劉可慧、彭少麟、莫江明、黃忠良、方運霆(2005)酸沉降對森林植物影響過程和機理。生態環境 14(6): 953-960。
劉瓊霦、許博行(1999)幹流水及穿落水的水量及水質在三種林分的變化。林業研究季刊 21(2): 51-59。
劉瓊霦(2000)雨水流經關刀溪三種林分水化學的變化。國立中興大學森林系研究所博士論文。42-51頁。
薛美莉(2000)台灣中部山區降水質及酸性沈降。特有生物研究 2: 21-33。
嚴重玲、洪葉湯、林鵬、王世杰、付舜珍、楊先科、吳善綺、朱克勇(2000)酸雨脅迫下稀土元素對小麥生理生化影響的效用。自然科學進展 10(1): 80-84。
Barnes, J. D., L. Balauger, E. Manrique, S. Elvirra and A. W. Davison(1992)A reappraisal of the use of DMSO for the extraction and determination of chlorophyll a and b in lichens and higher plants. Environmental Experimental Botany 32(2): 85-100.
Beckerson, D. W. and G. Hofstra(1979)Effect of sulphur dioxide and ozone singly or in combination on leaf chlorophyll, RNA, and protein in white bean. Canadian Journal of Botany 57: 1940-1945.
Bellot, J. and A. Escarre(1991)Chemical characteristics and temporal variations of nutrients in throughfall and stemflow of three species in Mediterranean holm oak forest. Forest Ecology and Management 41: 125-135.
Bowler, C., M. V. Montagu and D. Inze(1992)Superoxide dismutase and stress tolerance. Annual Review of Plant Physiology and Plant Molecular Biology 43: 83-116.
Cheng, F. Y., K. O. Burkey, J. M. Robinson and F. L. Booker(2007)Leaf extracellular ascorbate in relation to O3 tolerance of two soybean cultivars. Environmental Pollution 150(3): 355-362.
Chia, S., C. I. Mayfield and J. E. Thompson(1984)Simulated acid rain induces lipid peroxidation and membrane damage in foliage. Plant, Cell and Environment 7:333-338.
Clark, K. L., N. M. Nadkarni, D. Schaefer, H. L. Gholz(1998)Atmospheric deposition and net retention of ions by the canopy in a tropical montane forest, Monteverde, Costa Rica. Journal of Tropical Ecology 14: 27-45.
DeHayes, D. H., P. G. Schaberg, G. J. Hawley and G. R. Strimbeck(1999)Acid rain impacts on calcium nutrition and forest health. Bioscience 49(10): 789-800.
De Jong, DW.(1972)Detergent extraction of enzymes from tobacco leaves varying in maturity. Plant Physiology 50: 733-737.
Dixon, M. J. and A. L. Kuja(1995)Effects of simulated acid rain on the growth, nutrition, foliar pigment and photosynthetic rates of sugar maple and white spruce seedling. Water, Air and Soil Pollution 83: 219-236.
Eamus, D. and D. Fowler(1990)Photosynthetic and stomatal conductance responses to acid mist of red spruce seedlings. Plant, Cell and Environment 13:349-357.
Elstner, E. F.(1982)Oxygen activation and oxygen toxicity. Annual Review of
Plant Physiology 33: 73-96.
Erice, G., I. Aranjuelo, J. J. Irigoyen and M. SanchezDiaz(2007)Effects of elevated CO2, temperature and limited water supply on antioxidant status during regrowth of nodulated alfalfa. Physiologia Plantarum 130: 33-45.
Evans, L. S., N. F. Gmur and F. D. A. Costa(1978)Foliar response of six clones of hybrid poplar to simulated acid rain. Phytopathology 68(8): 847-856.
Feierabend, J., C. Schaan and B. Hertwig(1992)Potoinactivation of catalase occur under both high and low temperature stress conditions and accompanies photoinhibition of photosystem II. Plant Physiology 100: 1554-1561.
Foster, J. G. and J. L. Hess(1980)Response of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to and atmosphere enriched in oxygen. Plant Physiology 66: 482-487.
Frankel, E. N.(1985)Chemistry of free radical and singlet oxidation of ipids. Progress in Lipid Research 23 : 197-221.
Friend, A. L., P. T. Tomlinson, R. E. Dickson, E. G. O’neill, N. T. Edwards and G. E. Taylor(1992)Biochemical composition of lobbolly pine reflects pollutant exposure. Tree Physiology 11:35-47.
Gabara, B., M. Sklodowska, A. Wyrwicka, S. Glinska and M. Gapinska(2003)Changes in the ultrastructure of chloroplasts and mitochondria and antioxidant enzyme activity in Lycopersicon esculentum Mill. Leaves sprayed with acid rain. Plant Science 164: 507-516.
Gordeeva, A. V., R. A. Zevyagilskaya and Y. A. Labas(2003)Cross-talk between reactive oxygen species and calcium in living cells. Biochemistry 68(10): 1077-1080.
Hansen, U., B. Fiedler and B. Rank(2002)Variation of pigment composition and antioxidative systems along the canopy light gradient in a mixed beech/oak forest:
a comparative study on deciduous tree species differingin shade tolerance. Trees 16: 354-364.
Hodges, D. M, J. M. Delong, C. F. Forney and R. K. Prange(1999)Improving the thiobarbituric acid-reactive substances assay for estimating lipid peroxidation in plant tissue containing anthocyanin and other interfering compounds. Planta 207: 604-611.
Igawa, M., H. Kameda, F. Maruyama, H. Okochi and I. Otsuka(1997)Effects of simulated acid fog on needles of fir seedlings. Environment and Experimental Botany 38: 155-163.
Jana, S. and M. Choudhuri(1982)Glycolate metabolism of three submerged aquatic angiosperms during aging. Aquatic Botany(12): 345-354.
Klapheck, S., I. Zimmer and H. Cosse(1990)Scavenging of hydrogen peroxide in the endosperm of Ricinus communis by ascorbate peroxidase. Plant Cell Physiology 31(7): 1005-1013.
Kiyoshi, T., S. Yoshiko, K. Noriaki and S. Kiyoshi(1985)O3 tolerance and the ascorbate-dependent H2O2 Decomposing system in chloroplasts. Plant Cell Physiology 26(7): 1425-1431.
Kukavica, B. and S. V. Jovanovic(2004)Senescence-related changes in the antioxidant status of ginkgo and birch leaves during autumn yellowing. Physiologia Plantarum 122: 321-327.
Kumar, G. N. and N. R. Knowles(1993)Changes in lipid peroxidation and lipolytic and free-radical scavenging enzyme activities during aging and sprouting of potato(Solanum tuberosum)seed-tubers. Plant Physiology 102: 115-124.
Larson, R. A.(1988)The antioxidants of higher plants. Phytochemistry 27: 969-978.
Law, M. Y., S. A. Charles and B. Halliwell(1983)Glutathione and ascorbate acid in spinach(Spinacia olerocea)chloroplasts. Biochemical Journal 210: 899-903.
L’Hirondelle, S. J., J. S. Jacobson and J. P. Lassoie(1992)Acid mist and nitrogen fertilization effects on growth, nitrate reductase activity, gas exchange, and frost hardiness of red spruce seedling. New Phytologist 121: 611-622.
Lopez-Huertas, E(2002)Stress induces peroxisome biogenesis gene. EMBO Journal 19: 6770-6777.
Macadam, J. W., C. J. Nelson and R. E. Sharp(1992)Peroxidase activity in the leaf elongation zone of tall fescue. Plant Physiology 99: 872-878.
Mann, L. K, S. B. McLaughlin and D. S. Shriner(1980)Seasonal physiological responses of white pine under chronic air pollution stress. Environment and Experimental Botany 20: 99-105.
Martinez-Dominguze D., M. A. de las Heras, F. Navarro, R. Torronteras, F. Cordoba(2008)Efficiency od antioxidant response in Spartina densiflora: An adaptative success in a polluted environment. Environment and Experimental Botany 62: 69-77.
McClung, C. R.(1997)Regulation of catalase in Arabidopsis. Free Radical Biology and Medicine23: 489-496.
Mittler, R.(2002)Oxidative stress, antioxidants and stress tolerance. Trends Plant Science 7 : 405-410.
Morrison, I. K.(1984)Acid rain: A review of literature on acid deposition effects in forest ecosystems. Forest Abstracts 45(8): 483-506.
Nakano, Y. and K. Asada(1981)Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiology 22: 867-880.
Neill, S., R. Desikan and J. Hancock(2002)Hydrogen peroxidase signaling.Current Opinion in Plant Biology 5: 388-395.
Noctor, G and C. Foyer(1998)Ascorbate and glutathione: keeping active oxygen under control. Annual Review Plant Physiology and Plant Molecular Biology 49: 249-279.
Raffaella, B., S. Arisci, M. C. Brizzio, R. Mosello, M. Rogora, A. Tagliaferri(2007)Dry deposition of particles and canopy exchange: Comparison of wet, bulk and throughfall deposition at five forest site in Italy. Amospheric Environment 41: 745-756.
Scandalios, J. G.(1993)Oxygen stress and superoxide dismutases. Plant Physiology 101: 7-12.
Sheppard, L. J., I. D. Leith, M. B. Muarry, J. N. Cape and V. H. Kennedy(1998)The response of Norway spruce seedlings to simulated mist. New Phytologist 124: 595-605.
Takahama, U, M. Hirotusa and T. Oniki(1999)Age-dependent change in levels of ascorbatic acid and chlorogenic avid and activities of peroxidase and superoxide dismutasein the apoplast of tobacco leaves: Mechanism of oxidation of chlorogenic acid and in the apoplast. Plant cell physiology 40: 716-724.
Tomlinson, G. H.(2003)Acidic deposition, nutrient leaching and forest growth. Biogeochemistry 65: 51-81.
Turunen, M., S. Huttunen(1991)Effects of simulated acid rain on the epicuticular wax of Scots pine needles under notherly conditions. Canadian Journal of Botany 69: 412-419.
Van Breusegem, F., M. Van Montagu, and D. Inze(1998)Engineering stress tolerance in maize. Outlook on Agriculture 27: 115-124.
Velikova, V., I. Yordanov, A. Edreva(2000)Oxidative stress and some antioxidant systems in acid rain-treated bean plants : Protective role of exogenous polyamines. Plant Science 151: 59-66.
Willekens, H., D. Inze, M. van Montagu and W. van Camp(1995)Catalase in plants. Molecular Breeding 1: 207-228.
Woo, S. Y., D. K. Lee and Y. K. Lee(2007)Net photosynthesis rate, ascorbate peroxidase and glutathione reductase activities of Erythrina orientalis in polluted and non-polluted areas. Photosynthetica 45(2): 293-295.
Wyrwicka, A. and M. Sklodowska(2006)Influence of repeated acid rain treatment onantioxidative enzyme activities and on lipid peroxidation in cucumber leaves. Environmental and Experimental Botany 56: 198-204.
Yu, J. Q., S. F. YE and L. F. Huang(2002)Effects of simulated acid precipitation on photosynthesis, chlorophyll fluorescence, and antioxidative enzymes in Cucumis sativus L. Photosynthetica 40(3): 331-335.
Zeng, G.. M., G.. Zhang, G. H. Huang, Y. M. Jiang, H. L. Liu(2005)Exchange of Ca2+ , Mg2+ and K+ and uptake of H+, NH+ for the subtropical forest canopies influenced by acid rain in Shaoshan forest located in Central South China. Plant Science 168: 259-266.
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