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研究生:林亮瑩
研究生(外文):Liang-Ying Lin
論文名稱:台灣魚腥草(HouttuyniacordataThunb.)收集系揮發油變異性之研究
論文名稱(外文):Variation of volatile compounds in the collections of Houttuynia cordata Thunb. collected from Taiwan
指導教授:胡澤寬
學位類別:碩士
校院名稱:國立中興大學
系所名稱:農藝學系所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:59
中文關鍵詞:魚腥草揮發油甲基正壬酮
外文關鍵詞:Houttuynia cordata Thunb.volatile oilmethyl-n-nonyl ketone
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本試驗以台灣魚腥草35個收集系為材料,調查地上部揮發油含量及不同植株部位揮發油成分含量之周年變化及其在期作間之差異,並分析揮發油成分與組織特徵及農藝性狀間之相關關係以作為台灣魚腥草栽培利用與選拔育種之參考。
由分析結果顯示,台灣魚腥草地上部鮮物產量高於地下莖,揮發油含量則呈地上部高於地下莖,但無論植株部位皆以春作高於秋作。分析不同植株部位的揮發油及其成分之含量得知,月桂烯含量及產量呈地上部高於地下莖,甲基正壬酮、α-蒎烯、β-蒎烯及檸檬烯含量及產量則大多呈地下莖高於地上部,而地上部之α-蒎烯、β-蒎烯及檸檬烯含量極微量或幾近無法檢測。調查魚腥草地上部揮發油之周年變異可知,揮發油及其活性成分含量均在開花期(六月)達到最高峰,隨即快速降低,經七月底收割後,揮發油成分含量逐漸上升,至十一月為高峰,隨即又降至最低。
進一步比較35個收集系揮發油及其成分含量變異與期作間差異,經變方分析結果顯示,地上部揮發油及其成分含量,無論在收集系間、季節間均呈極顯著差異,而且季節與收集系間也具有顯著之交感效應。在鮮物產量方面,秋作所有收集系平均之鮮物產量高於春作,春作以恆春-2收集系為鮮物產量最高,成功及蘇澳收集系為最低,秋作則以萬華收集系鮮物產量最高,陽明山收集系為最低。在揮發油含量方面,春作所有收集系平均之揮發油含量高於秋作,春作以龍潭及新社收集系揮發油含量最高,蘇澳收集系為最低,秋作以龍潭及水林收集系揮發油含量最高,蘇澳收集系則最低。在甲基正壬酮與月桂烯含量及單位面積產量方面,所有收集系之平均活性成分含量呈春作高於秋作。春作甲基正壬酮單位面積產量以新社收集系最高,而成功和陽明山收集系最低;月桂烯單位面積產量則以龍潭收集系為最高,國姓收集系最低。秋作甲基正壬酮單位面積產量以水林收集系最高,而基隆收集系最低;月桂烯的含量則以龍潭和長濱-2為最高,蘇澳收集系爲最低。在地上部油細胞方面,組織的油細胞之分布與數量因收集系不同而有差異,油細胞數變異以地上莖為大,葉片次之,中肋最小。
根據揮發油相關分析結果,地上部鮮重與揮發油含量及月桂烯含量呈顯著正相關;地上部揮發油含量與甲基正壬酮、月桂烯含量之間呈極顯著之正相關群;甲基正壬酮含量與葉片的油細胞數呈顯著正相關;葉片油細胞數則與中肋油細胞數呈極顯著正相關。35個收集系依地上部鮮重、揮發油含量及甲基正壬酮含量等三個性狀在春作可區分成4群,而秋作則分成5群;其中,龍潭收集系無論春秋作之地上部鮮物產量、揮發油含量與甲基正壬酮含量皆高,而蘇澳收集系則為最低。
The thirty-five collections of Houttuynia cordata Thunb. collected in Taiwan were used as materials to investigated the variation of plant parts , cropping seasons and growth stages for the content of volatile oils and oil components in the aerial part. In addition, the estimators such as correlation analysis for volatile oil components and anatomic characters were used to reference.
The results follow that after investigation, the weights of fresh rhizome in thirty-five collections were larger than fresh aerial part, but content of volatile oil in the aerial part were larger than in the rhizome. Content of volatile oil in the aerial part and rhizome were at the highest level in spring cropping, especial at flowering and fruiting stage. After analysis of volatile oil components content in different plant part, the content of myrcene was as follows in the aerial part more than in rhizome, the contents of methyl-n-nonyl ketone, α-pinene, β-pinene, and d-limonene were as follows in rhizome more than in the aerial part, and the contents of α-pinene, β-pinene, and d-limonene in the aerial part of most collections were almost undetectable. In life cycle, the contents of all volatile oil components in the aerial part were at the highest level at flowering stage (in June) and therewith lost quickly. When end of July, plants were cut, the contents of all volatile oil components in the aerial part increase gradually, and at the high level in November, and therewith at the lowest level.
The results of ANOVA indicate that the treatment of collections, cropping seasons and the interaction of collections and cropping seasons get a significant level for volatile oils content and volatile oil components content of the aerial part. Mean of fresh aerial part weights of all collections in fall cropping was larger than mean in spring cropping. In spring cropping, collection 35 was at the highest level and collection 6, 31 were at lowest level for fresh aerial part weights. In fall cropping, collection 3 was at the highest level and collection 5 were at lowest level for fresh aerial part weights. Mean of volatile oils content for all collections in spring cropping was larger than mean in fall cropping. In spring cropping, collection 7, 18 were at the highest level and collection 6 was at lowest level for volatile oils content; in fall cropping, collection 7, 26 were at the highest level and collection 6 was at lowest level for volatile oils content. Mean of methyl-n-nonyl ketone and myrcene contents for all collections in spring cropping was larger than mean in fall cropping. In spring cropping, collection 18 was at the highest level and collection 5, 31 were at lowest level for methyl-n-nonyl ketone content; collection 7 was at the highest level and collection 24 was at lowest level for myrcene content. In fall cropping, collection 26 was at the highest level and collection 1 was at lowest level for methyl-n-nonyl ketone content; collection 7, 30 were at the highest level and collection 6 was at lowest level for myrcene content. Distributes and numbers of oil cell in tissues of the aerial part differed with collections, coefficient of oil cell numbers variation in stem was larger than in leaf, and coefficient of oil cell numbers variation in midrib was smallest.
According to correlation analysis for volatile oils, fresh weight of the aerial part was reciprocal positively correlated with content of volatile oils and content of myrcene; content of volatile oils of the aerial part was reciprocal positively correlated with contents of methyl-n-nonyl ketone and myrcene. Content of methyl-n-nonyl ketone was positively correlated with oil cells in leaf; oil cells in leaf were reciprocal positively correlated with oil cells in midrib. The thirty-five collections could be distinguish into four groups and five groups respectively by weights of fresh aerial part, content of volatile oils, and content of methyl-n-nonyl ketone in spring and fall cropping. In spring or fall cropping, collection 7 was at the highest level and collection 6 was at the lowest level for fresh weights of the aerial part, content of volatile oils and it’s components in all collections.
章節 頁次
中文摘要………………………………………………………………i
英文摘要………………………………………………………………iii
目錄……………………………………………………………………v
表目錄…………………………………………………………………vi
圖目錄…………………………………………………………………vii
前言……………………………………………………………………1
前人研究………………………………………………………………3
材料與方法……………………………………………………………9
結果
ㄧ、魚腥草不同植株部位間揮發油及其成份含量之變異…………17
二、魚腥草地上部揮發油及其成份含量之周年變化………………28
三、魚腥草揮發油及其成分在不同收集系間及春秋作之變異……33
四、魚腥草組織中油細胞與揮發油之相關性………………………46
討論……………………………………………………………………52
參考文獻………………………………………………………………55
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