黃精自古以來被認為是平日疲憊或病後體弱之藥食皆可的藥用作物，台灣黃精(Polygonatum altelobatum)日漸採集不易，人工栽培需求日殷，卻未見組織培養繁殖報導。本試驗目的為建立台灣黃精組織培養繁殖體系，供日後人工栽培需大量種苗繁殖之用。台灣黃精地下塊莖取自苗栗縣南庄鄉，利用修正的MS培養基進行無菌苗建立、葉片繁殖、地上部增殖與發根誘導等試驗。

試驗結果如下：
1.地下塊莖是初期建立無菌苗的優良材料，基部葉片則差。
2.試管苗剝去1-2層外葉後，葉片培養可以獲得芽體再生，此舉可以
大幅增加材料來源。培養基添加3-5 mg/l BA 與 20-40 mg/l
adenine sulfate 明顯提高葉片培養的芽體形成率與芽體數。
3.培養基添加0.1 mg/l PPT更明顯促進葉片基部膨大、芽體形成率
與芽體數。
4.芽體培養於含0.1-0.2 mg/l GA，可促進芽體生長，此為誘導發根
前重要步驟之一。
5.藍光下，培植體傾向地上部生長；而紅光下，培植體傾向誘導發
根。
6.發根培養基以添加15g/l蔗糖為佳，但若再添加0.5 mg/l GA，則
蔗糖需求上升至25 g/l 為佳，而且發根率與根部生長未受限制，
此為提高出瓶前植株健全之優良方法。

本試驗完成台灣黃精試管內繁殖體系，總計有改良為利用葉片培養、添加PPT促進芽體形成率與芽體數、發根期間藍光處理與添加GA促進幼苗的整體生長等3大項，為以往同屬異種植物的報告所不及，對於台灣黃精試管內大量繁殖極具改善價值。

Huang-Jing, Polygonatum altelobatum Hay., a well known Chinese herbal medicine for thousands years. Papers on tissue culture of P. altelobatum are bare and quite limited. Our study emphasized on effects of plant growth regulators and light conditions on tissue culture of P. altelobatum. The results showed that for initial culture from different parts of mature plant, the optimum concentration of BA at 3.07 mg/l in modified MS medium was highest shoot formation when tuber segments used as explants, and that for young leaf segments was 0.5 mg/l BA. Adenine sulfate also was used in initial culture and the optimum concentration was at 24 mg/l for the highest shoot formation of explants derived from young-leaf explants, and that for tuber explants is at 19.4 mg/l. For shoot multiplication, swollen-base leaf was decreased from 36.7% to 13.3% when phosphinothricin (PPT) concentrations increased from 0 to 0.4 mg/l. The optimum concentration of PPT at 0.11 mg/l got the highest number of shoot in tissue culture, and 0.13 mg/l PPT was optimum concentration for highest axillary bud formation. For shoot growth, GA3 at 0.103 mg/l and 0.095 mg/l were optimum for highest number of shoot formed from bud and swollen-base leaf explants, respectively. Longest shoot occurred when buds and swollen-base leaf used as explants cultured in medium with GA3 at 0.12 mg/l. For root development, root growth response and number of root per explants were significantly increased when the doses of NAA increased regardless of under blue or red light. Optimum concentration of sucrose from 26.75 g/l to 30.5 g/l is highest effective for leaf development and plantlet grew under blue light. The sucrose at 30.31 g/l under red light is suited for the highest number of root in tissue culture of P. altelobatum. The optimum concentration of GA3 at 23.33 g/l to 24.93 g/l is suite for effective of leaf and root development of P altelobatum plantlet.

Contents i
Content of Tables iv
Content of Figures vii
Abbreviations viii
Abstract ix
Chinese abstract xi
Chapter 1. Introduction 1
Chapter 2. Literature review 3
2.1. Plant tissue culture 3
2.2. Basic medium of tissue culture 5
2.3. Temperature and light 8
2.4. Plant growth regulators (PGRs) 9
2.4.1. Cytokinin 10
2.4.2. Auxins 11
2.4.3. Gibberellins (GA3) 13
2.5. Effect of plant growth regulators on plant tissue culture 13
2.6. Rooting 14
2.7. Acclimatization 16
Chapter 3. Materials and methods 18
3.1. Materials 18
3.2. Culture mediums 18
3.3. Methods 20
3.3.1. Initial cultures 20
3.3.1.1. Effect of BA on initial culture of tuber and young leaves 20
3.3.1.2. Effect of adenine sulfate on growth of shoot derived from initial culture 21
3.3.2. Shoot multiplication 21
3.3.2.1. Effect of PPT on formation of swollen-base leaf 22
3.3.2.2. Effect of PPT on shoot multiplication 23
3.3.3. Effect of GA3 on shoot growth of plantlet 23
3.3.4. Root development 24
3.3.4.1. Effects of NAA and light on root development 24
3.3.4.2. Effect of sucrose and light on root development 25
3.3.4.3. Effect of sucrose and GA3 on root development 26
Chapter 4. Results 27
4.1. Initial culture 27
4.1.1. Effect of BA on initial culture of tuber and young leaves 27
4.1.2. Effect of adenine sulfate on growth of shoot derived from initial culture 28
4.2. Shoot multiplication 33
4.2.1. Effect of PPT on formation of swollen-base leaf 33
4.2.2. Effect of PPT on shoot multiplication 33
4.3. Effect of GA3 on shoot growth of plantlet 38
4.4. Root development 40
4.4.1. Effects of NAA and light on root development 40
4.4.2. Effects of sucrose on fresh weight and ratio of dry/fresh weight 45
4.4.3. Effect of sucrose and light on roots development 47
4.4.4. Effect of sucrose and GA3 on root development 51
Chapter 5. Discussion and Conclusion 54
5.1. Initial culture 54
5.1.1. Effect of BA on initial culture of tuber and young leaves 54
5.1.2. Effect of adenine sulfate on growth of shoot derived from initial culture 55
5.2. Shoot multiplication 57
5.3. Effect of GA3 on shoot growth of plantlet 58
5.4. Root development 59
5.4.1. Effects of NAA and light on root development 59
5.4.2. Effects of sucrose on fresh weight and ratio of dry/fresh weight 61
5.4.3. Effect of sucrose and light on roots development 61
5.4.4. Effect of sucrose and GA3 on root development 62
Conclusion 62
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