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研究生:黃奐嘉
研究生(外文):Huan-Chia Huang
論文名稱:利用RAPD及AFLP分析竹子之遺傳差異
論文名稱(外文):Application of RAPD and AFLP Analysis for Bamboo Genetic Diversity
指導教授:古新梅
學位類別:碩士
校院名稱:國立中興大學
系所名稱:農藝學系所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:41
中文關鍵詞:竹子隨機擴增片段多形性DNA片段長度多形性親緣關係
外文關鍵詞:bamboorandom amplified polymorphic DNAAmplified fragment length polymorphismgenetic diversity
相關次數:
  • 被引用被引用:2
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  • 收藏至我的研究室書目清單書目收藏:1
在竹子的分類研究上,一直以來都是以外表型態特徵做為分類上的依據,而且由於外表型態容易受環境影響,因此造成品種鑑定上的不便。在本研究中,透過隨機擴增片段多形性DNA ( Random amplified polymorphic DNA , RAPD )技術以及擴增片段長度多形性( Amplified fragment length polymorphism,AFLP ),分別以15個RAPD引子及20組AFLP引子對針對22種台灣現有的竹子營養系進行DNA鑑定及分類。
經RAPD 與AFLP分子標誌分析比較後發現,單獨以AFLP進行分群分析比單獨以RAPD進行分析更能得到明確的分群結果,大致上同屬的竹子都可被歸在相近的一群中;若是同時以RAPD與AFLP進行分群分析,有效分群的效果則更明顯。
經研究後,在梨果竹屬的部分我們發現在分子分析結果上親緣關係是與麻竹屬的相似度對高,而在麻竹屬與蓬萊竹屬的部份也都可被有效的分群。此外,本研究的分群結果也可與傳統的分類方式做結合,可使竹子的傳統分類上的準確度更加提升。
At present, taxonomic research on bamboo was depended on morphological characteristics which were easily effected by environment. Two PCR-based DNA fingerprinting techniques including 15 RAPD (random amplified polymorphic DNA) markers and 20 AFLP (amplified fragment length polymorphism) markers were used to investigate genetic diversity among 21 different bamboo species.
After clustering analysis with UPGMA of each marker system, the combination of both two marker systems could obtain better results than individual marker system. In summary, Melocanna baccifera was similar to Dendrocalamus, and Dendrocalamus, Bambusa could be well distinguished.
中文摘要....................................................................................................i
英文摘要....................................................................................................ii
目次...........................................................................................................iii
圖目次........................................................................................................v
表目次.......................................................................................................vi
壹、 前言………………………………………………………………1
貳、 前人研究........................................................................................2
ㄧ、 竹子簡介……………….………………………………….……2
二、 隨機擴增片段多形性DNA( Random Amplified
Polymorphism DNA,RAPD )……………………………….…2
三、 擴增片段長度多形性(Amplified Fragment Length Polymorphism,AFLP )………………………………….……..3
四、 分子標誌在植物親緣關係研究上之應用………………...…..3
五、 分子標誌在竹子上之應用………………………………….…4
參、 材料方法........................................................................................6

ㄧ、 試驗材料………………………………………………………6
二、 竹子葉片基因組DNA之萃取………………………………...6
三、 隨機擴增片段多形性分析………………………………….....7
四、 擴增片段長度多形性分析………………………………….....7
五、 聚丙醯胺膠體電泳及銀染法……………………………….....8
六、 多型性多型性 (polymorphic) 分析與
樹狀圖 (Dendrogram) 建立......................................................9
肆、 結果..............................................................................................10
ㄧ、 RAPD分析.............................................................................10
二、 AFLP分析..............................................................................10
三、 RADP與AFLP共同分析.....................................................13
伍、 討論..............................................................................................17
陸、 結論與未來展望..........................................................................21
柒、 參考文獻......................................................................................22
捌、 圖表...............................................................................................25
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