跳到主要內容

臺灣博碩士論文加值系統

(3.231.230.177) 您好!臺灣時間:2021/07/27 09:30
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:宋安娜
研究生(外文):Anchana Songkaew
論文名稱:外源性促性腺激素對發身前與發身後貓隻下視丘-腦下垂體-性腺調控的作用
論文名稱(外文):The Effect of Gonadotropin-Releasing Hormone on the Regulation of Hypothalamus-Pituitary Axis in Prepuberty and Puberty Cats
指導教授:李衛民李衛民引用關係
學位類別:碩士
校院名稱:國立中興大學
系所名稱:獸醫學系暨研究所
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:英文
論文頁數:52
中文關鍵詞:發身腦下垂體
外文關鍵詞:PrepubertyPubertycatGnRH
相關次數:
  • 被引用被引用:0
  • 點閱點閱:88
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
許多品種的動物,促性腺激素刺激試驗是一種常常用來評估異常生殖功能的診斷方法,這些動物當然也包括貓。但是到目前為止,卻沒有針對正常貓隻對於促性腺素刺激試驗的報告。因此,此篇論文的目的是在評估發身前公貓與姆貓對於外源的促性腺激素刺激試驗的反應,此外也針對發身後公貓與母貓對於不同劑量外源的促性腺激素刺激試驗的反應。於發身前,血液樣本的收集分別於給予10 µg/kg 外源的促性腺激素之前與給予10 µg/kg外源的促性腺激素後各時間點,收集後的血液樣本,於公貓則檢測睪固酮,母貓則檢測發情素與助孕酮。另外,針對發身後貓隻對於不同劑量的性腺刺激素刺激試驗的反應,分別給予 20 與 40 µg/kg性腺刺激素,然後收集血液樣本 ,於公貓檢測睪固酮的濃度,於母貓則檢測發情素與助孕酮的濃度。此次試驗的結果顯示,無論是在發身前或是發身後,公貓對於外源性促性腺激素都會有反應,於給予促性腺激素60分鐘與120分鐘後的時間點,都顯著高於給予促性腺激素之前或是給予後10分鐘。但是卻沒有劑量依賴的現象,也就是說睪固酮的濃度並不會因為促性腺激素的濃度增加而增加。. 平均血漿發情素濃度在給予 10 µg/kg 促性腺激素後,於發身前的濃度為,發身後為,兩者並沒有顯著差異。同時,個體對於促性腺激素刺激後的反應也不盡相同,因而推論具有個體差異,但是原因不明。此外,助孕酮的濃度都低於 1.5 ng/ml ,無論是在給予促性腺激素之前或是之後。因此本實驗顯示在發身前與發身後的公貓對於促性腺激素會有較高的反應,而非母貓,這可能為腦下垂體對於外源性促性腺激素的調控,從發身前就已經不同了。
The exogenous gonadotropin stimulation (GnRH) test is diagnostic method used to evaluate the abnormal reproductive functions in many species, including cat. However, the GnRH challenge test in normal cat has not been studies. Therefore, the aims of this study were to evaluate the response of ovarian and testis hormones to exogenous gonadotropin in nine prepubertal and pubertal cats and to evaluate the dosage response of exogenous gonadotropin in pubertal cats. The blood samples were collected at time before and multiple times after 10 µg/kg GnRH intravenous administration for determining the concentration of estradiol-17β, progesterone, and testosterone. Twenty and forty µg/kg GnRH were administrated intravenous in the same pubertal cats for evaluation of dosage response. The mean plasma testosterone before and 10 minutes were significantly lower than 60 and 120 minutes after 10 µg/kg GnRH administration in prepubertal cats and in male pubertal cats after 10, 20, and 40 µg/kg GnRH administration. In high dosage of GnRH, 20 and 40 µg/kg GnRH could not induce an increase of plasma testosterone concentrations when compared with 10 µg/kg GnRH. The means plasma estradiol in prepubertal and pubertal female cats after 10 µg/kg GnRH administration were 18.4±4.45 and 14.40±5.89 pg/ml respectively. The mean plasma estradiol-17β between before and after GnRH administration was not significant in all dosage of GnRH. Moreover, the response to GnRH in each cat was individual. Additionally, the mean plasma progesterone in all pubertal female cats was lower than 1.5 ng/ml both before and after GnRH administration. In conclusion this study indicated the different of response to exogenous GnRH in prepubertal male and female cats. it may be ascribed to the regulation of supra-pituitary in prepubertal male and female cats.
Abstract...............................................i
Table of contents .................... ............... iii
List of Figures........................................v
List of Tables ........................................viii
Chapters:
1. Preface..............................................1
2. Literature Review..................................................2
2.1 Introduction........................................2
2.2 An Overview of the Hypothalamic-Pituitary Gonadal Axis in MammalSpecies........................................2
2.3 Hormone of the Gonads...............................5
2.3.1 Estrogens.........................................5
2.3.2 Progesterone......................................5
2.3.3 Androgen..........................................6
2.4 Reproductive Physiology in Female Cat...............6
2.4.1 The Kitten........................................6
2.4.2 The Queen.........................................7
2.4.3 Stage of Estrus Cycle.............................8
2.5Reproductive Physiology in Male Cat..................9
2.5.1 Male Puberty......................................9
2.6 Gonadotropin-releaseing hormone test (GnRH test)
2.6.1 Summary of GnRH Tests for Assessing Reproductive
Status in Female........................................10
2.6.2 Summary of GnRH Stimulation for Assessing Reproductive Status in mal..............................12
2.7 Objective of our study..............................13
3. Materials and Methods................................15
3.1Experimental animals Androgen........................17
3.2Experiment design....................................17
3.3 Experiment 1; GnRH stimulation test in different age of cats....................................................18
3.4 Experiment 2; Dose-response to GnRH in puberty cat..18
3.5 Determination of testicular testosterone by enzyme immunoassay.............................................19
3.6 Determination of estradiol-17β by enzyme immunoassay.............................................19
3.7 Determination of progesterone by enzyme immunoassay.............................................20
3.8 Statistical analyses................................20
4..Results..............................................21
3.1 Experiment 1........................................21
3.1.2 Estradiol-17β....................................21
3.1.3Testosterone.......................................21
3.2 Experiment2.........................................22
3.2.1 Estradiol-17β....................................22
3.2.1 Progesterone......................................22
3.2.2 Testosterone......................................23
5.Discussion............................................41
6. Conclusion...........................................46
Reference...............................................47
Adams GP, Evans ACO, Rawlings NC. Follicular waves and circulating gonadotropins in 8-mounth-old prepubertal heifers. J Reprod Fertil 100: 27-33, 1994.
Anderson AC. The beagle as an experimental dog. Iowa: Iowa state University Press. 1970.
Axner E, Gustavsson T, Holst BS. Estradiol measurement after GnRH-stimulation as a method to diagnose the presence of ovaries in the female domestic cat. Theriogenology 70: 186-191, 2008.
Bakker J, Baum MJ. Neuroendocrine regulation of GnRH release in induced ovulators. Front Neuroendocrinol 21: 220–262, 2000.
Bartlewski PM, Beard AP, Cook SJ, Rawlings NC. Ovarian activity during sexual maturation and following introduction of the ram to ewe lambs. Small Rumin Res 43: 37-44, 2002.
Bearden HJ, Fuquay JW, Willard ST. 6th ed. Applied animal reproduction. United state: Pearson education. 44-48, 2004.
Beckman DA, Feuston M. Landmarks in the development of the female reproductive system. Birth Defects Res 68: 137–143, 2003.
Beijerink NJ, Buijtels JJCWM, Okkens AC, Kooistra HS, Dieleman SJ. Basal and GnRH-induced secretion of FSH and LH in anestrous versus ovariectomized bitches. Theriogenology 67: 1039–1045, 2007.
Blanchard T, Dickson V, Corey M, Roser J. Recommendation for clinical GnRH challence testing of stallions. J Equine Vet Sci 20: 678-737, 2000.
Blottner S, Jewgenow K. Moderate seasonality in testis function of domestic cat. Reprod Dom Anim 42: 536-540, 2007.
Chastain CB, Ganjam VK. Clinical endocrinology of companion animals. Lea & Febiger: Philadelphia. 439-534, 1986.
Chatdarong K, Ponglowhapan S, Karlsson A, Forsberg C. The effect of ACTH stimulation on cortisol and progesterone concentrations in intact and ovariohysterectomized domestic cats. Theriogenology 66: 1482–1487, 2006.
Cuttler L, Rosenfield RL, Ehrhann DA, Kreiter M, Burstein S, Cara JF, Levitsky LL. Maturation of Gonadotropin and Sex Steroid Responses to Gonadotropin-Releasing Hormone Agonist in Male. J Clin Endocrinol Metab 76: 362-366, 1993.
Denardo GA, Becker K, Brown NO, Dobbins S. Ovarian remnant syndrome: revascularization of free-floating ovarian tissue in the feline abdominal cavity. J Am Anim Hosp Assoc 37: 290-296, 2001.
Dizier MS, Malandain E, Thoumire S, Remy B, Maillard SC. Expression of follicle stimulating hormone and luteinizing hormone receptors during Follicular growth in the domestic cat ovary. Mol Reprod Dev 74: 989–996, 2007.
Ettinger SJ, Feldman E. 6th ed. Textbook of veterinary internal medicine. St Louis: Elsevier. 1698-1707, 2005.
Genazzani1 AD, Petraglia F, Gastaldi M, Volpogni C, Gamba O, Massolo F, Genazzani AR. Evidence suggesting an additional control mechanism regulating episodic secretion of luteinzing hormone and follicle stimulating hormone in pre-pubertal children and post-menopausal women. Hum Reprod 9: 1807-1812, 1994.
Graham LH, Byers AP, Armstrong DL, Loskutoff NM, Brown JL, Wildt DE , Swanson WF. Natural versus chorionic gonadotropin-induced ovarian responses in the clouded leopard (Neofelis nebulosa) assessed by fecal steroid analysis. Biol Reprod 53: 93–102, 1995.
Heffelfinger DJ. Ovarian remnant in a 2-year-old queen. Can Vet J 47: 165-167, 2006.
Howard JG, Roth TL, Byers AP, Swanson WF, Wildt DE. Sensitivity to exogenous gonadotropins for ovulation induction and laparoscopic artificial insemination in the cheetah and clouded leopard. Biol Reprod 56: 1059-1068, 1997.
Howe LM, Slater MR, Boothe HW, Hobson HP, Fossum TW, Spann AC, Wilkie WS. Long-term outcome of gonadectomy performed at an early age or traditional age in cats. J Am Vet Med Assoc 217: 1661-1665, 2000.
Johnston SD, Root MV. Ovarian and testicular function in the domestic cat: clinical management of spontaneous reproductive disease. Anim Reprod Sci 42: 261-274, 1996.
Kutzler MA. Estrus induction and synchronization in canids and felids. Theriogenology 68: 354–374, 2007.
Memon MA, Ganjam VK, Pavletic MM, Schelling SH. Use of human chorionic gonadotropin stimulation test to detect a retain testis in a cat. J Am Vet Med Assoc 201: 1602, 1992.
Millar RP. GnRHs and GnRH receptors. Anim Reprod Sci 88: 5–28, 2005.
Miller DM. Ovarian remnant syndrome in dogs and cats: 46 cases (1988-1992). J Vet Diagn Invest 7: 572-574, 1995.
Munro EAC, Watson ED, Van D, Broek AH. Testosterone release in intact male dogs following the injection of a GnRH analogue. Vet Rec 139: 96, 1996.
Nakada K, Moriyoshi M, Nakao T, Watanabe G, Taya K. Changes in concentrations of plasma immunoreactive follicle-stimulating hormone, luteinizing hormone, estradiol-17b, testosterone, progesterone, and inhibin in heifers from birth to puberty. Domest Anim Endocrinol 18: 57–69, 2000.
Neubauer K, Jewgenow K, Fickel J. Follicle-stimulating hormone receptor in felids: intra- and interspecies variation. Theriogenology 66: 1737-1742, 2006.
Nieman LK. Reproductive Test in Females. Endotex.com. 2003.
Pelican KM, Wildt DE, Ottinger MA, Howard J. Priming with progestin, but not GnRH antagonist, induces a consistent endocrine response to exogenous gonadotropins in induced and spontaneously ovulating cats. Domest Anim Endocrinol 34: 160–175, 2008.
Pope CE. Embryo technology in conservation efforts for endangerd felids. Theriogenology 63: 163-174, 2000.
Rawling NC, Evans ACO, Honaramooz A, Bartlewski PM. Antral follicle growth and endocrine changes in prepubertal cattle, sheep and goats. Anim Reprod Sci 78: 259-270, 2003.
Reiter EO, Root AW, Duckett GE. The response of pituitary gonadotropes to a constant infusion of Luteinizing hormone-releasing hormone (LHRH) in normal prepubertal and pubertal children and in children with abnormalities of sexual development. J Clin Endocrinol Metab 43: 400-411, 1976.
Rijnberk A. Protocols for function tests. In: Rijnberk A, ed. Clinical endocrinology of dogs and cats- an illustrated text. Kluwer academic publisher: Dordrecht. 205-212, 1996.
Romagnoli S. Clinical approach to infertility in the queen. Proceedings of the ESFM feline congress. Stockom, 2002.
Rosenfield RL, Garibaldi LR, Moll GW, Watson AC, Burstein S. The rapid ovarian secretory response to pituitary stimulation by the gonadotropin-releasing hormone agonist nafarelin in sexual precocity. J Clin Endocrinol Metab 63: 1386-1389, 1986.
Roser JF, Hughes JP. Dose-response effects of GnRH on gonadotropins and testicular steroids in fertile and subfertile stallions. J Androt 13: 543-550, 1992.
Sabeur K, Ball BA, Nett TM, Balland HH, Liu IKM. Effect of GnRH conjugated to pokeweed antiviral protein on reproductive function in adult male dogs. Reproduction 125: 801-806, 2003.
Shille VM, Lundstrom KE, Stabenfeldt GH. Follicular function in the domestic cat as determined by estradiol-17β concentrations in plasma: relation to estrous behavior and cornification of exfoliated vaginal epithelium. Biol Reprod 21: 953-963, 1979.
Swanlund DJ, N''Diaye MR, Loseth KJ, Pryor JL, Crabo BG. Diverse testicular responses to exogenous growth hormone and follicle-stimulating hormone in prepubertal boars. Biol Reprod 53: 749-757, 1995.
Swanson WF, Wolfe BA, Brown JL, Jimenez TM, Riviere JE, Roth TL, Wildt DE. Pharmacokinetics and ovarian-stimulatory effects of equine and human chorionic gonadotropins administered singly and in combination in the domestic cat. Biol Reprod 57: 295-302, 1997.
Tsumagari S, Ishinazaka T, Watanabe G, Taya K, Ishiii M. Successful pragnancy following Gonadotrophin-releasing hormone analogus treatment in a previously infertile bitch. J Small Anim Pract 47: 213-215, 2006.
Tsutsui T, Makagawa K, Hirano T, Nagakubo K, Shinomiya M, Yamamoto K, Breeding season in female cats acclimatedunder a natural photoperiod and interval until puberty. J Vet Med Sci 66: 1129–1132, 2004.
Tsutsui T, Murao I, Kawakami E, Ogasa A, Stabenfeldt GH. Androgen concentration in the blood and spermatogenic function of tom cats during the breeding season. Nippon Juigaku Zasshi 52: 801-806, 1990.
Verhage HG, Beamer NB, Benner RM. Plasma levels of estradiol and progesterone in the cat during polyestrus, pregnancy and pseudopregnancy. Biol Reprod 14: 579-585, 1976.
Waal HAD. Regulation of puberty. Best Pract Res Clin Endocrinol Metab 16: 1-12, 2002.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top