跳到主要內容

臺灣博碩士論文加值系統

(3.236.28.137) 您好!臺灣時間:2021/07/25 20:44
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:陳忠信
研究生(外文):Chung-Hsin Chen
論文名稱:以大鼠全皮層切除之皮膚創傷動物模式評估數種敷料之功效
論文名稱(外文):Comparison the Wound Healing Effect of Different Dressings on the Full Thickness Skin Wound Model in Rats
指導教授:廖俊旺廖俊旺引用關係
指導教授(外文):Jiunn-Wang Liao
學位類別:碩士
校院名稱:國立中興大學
系所名稱:獸醫病理生物學研究所
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
畢業學年度:96
語文別:中文
論文頁數:120
中文關鍵詞:大鼠全皮層創傷動物模式敷料
外文關鍵詞:ratthe Full Thickness Skin Wound Modeldressing
相關次數:
  • 被引用被引用:0
  • 點閱點閱:421
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
急性創傷癒合是由數種漸進性的過程所組成包含以下四期:止血期(hemostasis)、炎症期(inflammation)、增殖期(proliferation)以及重塑期(remodeling),而在各時期可由特異性的生化標幟或免疫化學抗體來加以區分,如由表皮細胞與巨噬細胞所釋放的血管內皮生長因子(vascular endothelial growth factor, VEGF)可影響創傷之血管新生,或以PCNA ,(proliferative cell nuclear antigen, PCNA)抗體評估創緣細胞與上皮新生情形。本實驗比較醫用敷料 (WH2)、傳統敷料 (WH3) 以及新開發敷料 (WH4) 在不同創傷面積、換藥頻率、傷口表面菌相調查及對促進皮膚癒合功效。使用Sprague Dawley品系大鼠 (250-300 g) 切除部分背部皮膚後,在第3、7、10、14和21天分別觀察各組傷口癒合情形,以影像分析系統測量其傷口面積,且在上述四個時間點對傷口進行傷口釣菌、體重測量與傷口肉眼病變觀察。試驗結束犧牲後採取其背部創傷皮膚進行組織病理學檢查,並對肉芽組織的生成 (granulation tissue)、血管新生 (angiogensis) 與新生上皮 (reepithelializaiton) 的程度進行組織學評分,分析癒合皮膚組織氧化壓力之丙二醛 (malondialdehyde, MDA) 含量以評估脂質過氧化的程度,並偵測肉芽組織內生性抗氧化酵素總麩胺基硫 (total glutathione, TGSH) 酵素與超氧歧化酶(superoxide dismutase, SOD)的活性;另以免疫化學染色評估組織內細胞PCNA與VEGF抗體標幟,觀察其細胞的陽性訊號 (positive signal)。實驗結果顯示,在醫用敷料組 (WH2) 方面,以H物質顯示對傷口癒合較佳,且較能降低創口的氧化壓力;傳統敷料組 (WH3),對較大切創之傷口癒合較無顯著的效果;而新開發敷料 (WH4) 之對創口的修復則具有顯著性的增加。組織學觀察方面,新開發敷料組與陽性對照組所採集的皮膚樣本,其真皮層皆可見到因敷料纖維斷片引起的異物性肉芽腫炎症反應,與創傷區域較成熟的膠原纖維生成,且與陽性對照組相比,具有相似促進癒合效力。在免疫組織化學染色結果方面,處理組新生組織內皆可見到VEGF和PCNA陽性訊號,尤以WH4敷料處理組VEGF陽性訊號為最強。創傷表面菌相調查則在WH2組的N樣本物質無細菌生長外,其餘組別皆以Staphylococcus spp菌種為主要。
Acute wounds normally heal in a very orderly and efficient manner characterized by four phases including hemostasis, inflammation, proliferation and remodeling. Specific biological markers characterize healing of acute wounds. Likewise, unique biologic markers also characterize pathologic responses, for example, vascular endothelial growth factor (VEGF) is the most important mediator of the angiogenic phase of healing, and proliferative cell nuclear antigen (PCNA) is expressed on proliferative cell during wound healing. The aim of the present study was to compare wound healing effect of several test materials including medical (WH2 group), traditional (WH3 group), and developed (WH4 group) dressings by the full thickness skin wound healing model in rats. Briefly, twenty-five male SD rats (250-300 g) were divided several groups, and the full thickness open excision wound was made on the back of the rat. The treated schedules of several test materials were followed by the individual dressing group. On the postoperative days of 3rd, 7th, 14th, and 21th, wound areas were calculated by image system, and the microbial organisms were cultured from the wound surface. At the end of study, healing tissues were took to score the pathological changes, and determinated the level of malondialdehyde (MDA), glutathione peroxidase (GPx), and superoxide dismutase (SOD) for oxidative status. Further, the PCNA and VEGF immunochemistry markers were used to observe the positive signal of cells in the healing tissues. The present results revealed that the WH2 and WH4 dressings had beneficial effects on the wound healing including decrease areas and pathological scores of the wound. In the WH4 dressing treatment group, we found a foreign body giant cell reaction in the alginate fiber incorporated in skin tissue. At WH2 group dressings treatment, it also found that the MDA level, GPx and SOD enzyme activities, compared with control group, significantly reduced the oxidative stress in the wound healing tissues. Except, the N sample of WH2 dressing group did not isolate bacteria on the skin wound, and the other test samples of Staphylococcus spp were mainly isolated.
目 錄
頁次
中文摘要 ………………………………………………………………………… I
英文摘要 ………………………………………………………………………… II
目錄 ……………………………………………………………………………… III
圖次 ……………………………………………………………………………… IV
表次 ……………………………………………………………………………… VII
第一章 前言 …………………………………………………………….............. 1
第二章 文獻探討 ……………………………………………………….............. 2
第一節 皮膚的結構 ……………………………….....…............................ 2
第二節 創傷的癒合 …..…….………………….......................................... 7
第三節 創傷修復的動物模式 …..………………………………………... 14
第四節 氧化壓力與傷口癒合 …….………….………............................... 21
第五節 敷料的選擇 ……..…………………............................................... 23
第六節 影響傷口癒合的因子 ………………...………….......................... 25
第三章 研究目的與實驗設計 …………………...………................................... 29
第一節 實驗設計…………………………………………………………... 29
第四章 材料與方法 ……………………………………..……………................ 30
第一節 實驗材料與儀器 …..……………………………………............... 30
第二節 試驗樣品 …………………………………………………………. 32
第三節 全皮層切除動物模式 ………...………………………………….. 38
第四節 氧化壓力測定 …..………............................................................... 45
第五節 免疫化學染色 ……..……………………………………………... 50
第六節 傷口表面菌相調查 ………………………………………………. 52
第五章 試驗結果 ……………………………………………………….............. 53
第一節 動物實驗結果 ……..…………………………............................... 53
第二節 氧化壓力之評估 …………………................................................. 92
第三節 免疫化學染色結果 …………...………………………………….. 96
第四節 傷口表面菌相調查結果 …………………………………………. 99
第六章 討論 ………………………………………………………........................ 103
參考文獻 ………………………………………………………………………… 109
參 考 文 獻
王文憲。最新解剖生理學。合記圖書出版社。台北。363-370,2004。
王麗姿。傷口癒合的相關營養因素及護理。實用護理雜誌 18: 55-56,2002。
王曉芹、王貴波、李曉輝。創面敷料及其對癒合的影響研究進展。中國臨床康復 6: 574-575,2002。
付小兵。生長因數與創傷修復。人民軍醫出版社。北京。25-29,1991。
付小兵、王亞平。鹼性成纖維細胞生長因子加速小型豬背部創傷修復的實驗研究。中華創傷雜志 11: 134-136,1995。
付小兵、王德文。創傷修復基礎。人民軍醫出版社。北京。184-201,1997。
田健廣、夏照帆。創面敷料的研究進展。解放軍醫學雜誌 25: 470-471,2003。
光井武夫。新化妝品學。合記圖書出版社。台北。1-30,2004。
林麗真。傷口癒合。藥學雜誌 9: 1-19,1993。
呂峰州。人體內的自由基掃除劑穀胱甘肱 (GSH)。健康世界,1993。
何善台。整形外科學。合記圖書出版社。台北。4-9,2001。
李俊仁。實用外科學。金名出版社。台北。263-285,2002。
洪敏元、劉良慧、林育娟、何明聰、賴明華。當代生理學。華杏出版股份有限公司。台北。33-43,1999。
胡普紅、朱全剛、孫華軍、高瑋。醫用輔料的分類及特點。解放軍藥學學報 16: 147-167,2000。
胡明火、陳文元、付小兵。鹼性成纖維細胞生長因子對皮瓣存活的影響。中華整形燒傷外科雜志 14: 115-117,1998。
黃中洋。Superoxide dismutases醫療功效。台灣醫界34: 139-143,1991。
黃進發。食用菇的抗氧化SOD之研發與利用。食品工業32: 43-53,2000。
黃穎斐。生醫敷料及人工皮膚。科學發展 380: 24-29,2004。
劉振軒、何逸遷、張文發、祝志平、王綉真。H&E染色。組織病理染色技術與圖譜。86-89。養研所、竹南。1996。
華彬、柏連松。影響傷口的因素及促癒方法。中國中西醫結合外科雜誌 7: 62-64,2001。
Agren MS, Eaglstein WH, Ferguson MWJ, Harding KG, Moore K, Saarialho-Kere UK, Schultz GS. Causes and effects of the chronic inflammation in venous leg ulcers. Acta Derm Venereol 210: 3-17, 2000.
Ahamd S. Antioxidant mechanisms of enzymes and proteins. In: Oxidative stress and antioxidant defenses in biology Ahmad S.Chapman and Hall.Eds. International Thomaon Publishing Inc. New Yourk. 240-272, 1995.
Altavilla D, Galeano M, Bitto A, Minutoli L, Squadrito G, Seminara P, Venuti F.S., Torre V, Calo` M, Colonna M., Cascio PL, Giugliano G, Scuderi N, Mioni C, Leone S, Squadrito F. Lipid peroxidation inhibition by raxofelast improves angiogenesis and wound healing in experimental burn wound. Shock 24: 85–91, 2005.
Altavilla D, Saitta A, Cucinotta D, Galeano M, Deodato B, Colonna M, Torre V, Russo G, Sardella A, Urna G, Campo GM, Cavallari V, Squadrito G, Squadrito F. Inhibition of lipid peroxidation restores impaired vascular endothelial growth factor expression and stimulates wound healing and angiogenesis in the genetically diabetic mouse. Diabetes 50: 667-674, 2001.
Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci USA. 90: 7915-7922, 1993.
Artuc M, Hermes B, Steckelings UM, Grutzkau A, Henz BM. Mast cells and their mediators in cutaneous wound healing--active participants or innocent bystanders? Exp Dermatol 8: 1, 1999.
Ashcroft GS, Mills SJ, Flanders KC. Role of Smad3 in the hormonal modualtion of in vivo wound healing responses. Wound Repair Regen 11: 468-473, 2003.
Ashcroft GS, Mills SJ, Lei K. Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor. J Clin Invest 111: 1309-1318, 2003.
Ashcroft GS, Dodsworth J, van Boxtel E. Estrogen accelerates cutaneous wound healing associated with an increase in TGF-β1 levels. Nat Med 3: 1209-1215, 1997.
Ashcroft GS, Greenwell-Wild T, Horan MA, et al. Topical estrogeen accelerates cutaneous wound healing in aged humans associated with an altered inflammatory response. Am J Pathol 155: 1137-1146, 1999.
Ashcroft GS, Horan MA, Ferguson MW. Aging is associated with reduced deposition of specific extracellular matrix components, an up-regulation of angiogenesis, a and an altered inflammatory reponse in a murine incisional wound healing model. J Invest Dermatol 108: 430-437, 1997.
Ashcroft GS, Horan MA, Ferguson MW. Aging alters the inflammatory and endothelial cell adhesion molecule profiles during human cutaneous wound healing. Lab Invesy 78: 47-58, 1998.
Ashcroft GS, Yang X, Glick AB. Mice lacking Smad 3 show accelerated wound healing and an impaired local inflammatory response. Nat Cell Biol 1: 260-266, 1999.
Attwood AI. Calcium alginate dressing accelerates split skin graft donor site healing. Br J Plast Surg 42: 373-379, 1989.
Ballas CB, Davidson JM. Delayed wound healing in aged rats is associated with increased collagen gel remodeling and contraction by skin fibroblasts, not with differences in apoptotic or myofibroblast cell populations. Wound Repair Regen 9: 223-237, 2001.
Barmett AH, Odugbesan O. Seaweed-based dressings in the management of leg ulcers and other wounds. Intern Ther Clin Mon 9: 70-76, 1998.
Battegay EJ. Angiogenesis: mechanistic insights, neovascular diseases, and therapeutic prospects. J Mol Med 73: 333, 1995.
Bello Y, Phillips TJ. Recent advances in wound healing. J Am Med Ass Med Assoc 283: 716-718, 2000.
Berry DP, Bale S, Harding KG. Dressings for treating cavity wounds; J Wound Care 5: 10-17, 1996.
Blair SD, Jarvis P, Salmon M, McCollum C. Clinical trial of calcium alginate haemostatic swebs. Br J surg 77: 568-570, 1990.
Bolognia JL and Orlow SJ. Biology of melanocyte, In: Bolognia JL, Jorizzo J, and Rapini R, eds. Dermatology. Harcourt, London, 43-54, 2003.
Botchkarev VA, Paus R. Molecular biology of hair morphogenesis:develop-
ment and cycling. J Exp Zoolog B Mol Dev Evol 298: 164-180, 2003.
Bown SA, Coimbra M, Coberly DM, Chao JJ, Rohrich RJ. Oral nutritional supple- mentation accelerates skin wound healind: a randomized, placebo-controlled, double-arm, crossover study. Plast Reconstr surg 114: 237-244, 2004.
Boykin JV, Eriksson E, Pittman RN. In vivo microcirculation of scald burn and the progression of postburn dermal ischemia. Plast Reconstr Surg 66: 191-198, 1991.
Breuing K, Eriksson E, Liu P. Healing of partial thickness porcine skin wounds in a liquid environment. J Surg Res 52: 50-58, 1992.
Broadley KN, Aquino AM, Woud ward SC. Monospecific antibodies implicate basic fibroblast growth factor in normal wound repair. Lab Invest 61: 571-575, 1989.
Brogi E, Wu T Namiki A, Isner JM. Indirect angiogenic cytokines upregulate VEGF and bFGF gene expression in vascular smooth muscle cells, whereas hypoxia upregulates VEGF expression only. Circulation 90: 649-652, 1994.
Casey G. The importance of nutrition in wound healing. Nurs Stand 13: 51-56, 1998.
Chaturvedi V, Handa R, Rao DN, Wali JP. Estimation and significance of serum and synovial fluid malondialdehyde levels in rheumatoid arthritis. Ind J Med Res 109: 170-174, 1999.
Chen HY, Yen GC. Free radicals, antioxidant defenses and human health. Nutritional Sci J. 23: 105-121, 1998.
Chowe CK. Vitamin E and oxidative stress. Free Rad Biol Med 11: 215-232, 1991.
Clark RA. Fibrin and wound healing. Ann N Y Acad Sci 936: 355, 2001.
Cohen IK, Mast BA. Models of wound healing. J Trauma 30 (12Suppl.): S149-155, 1990.
Corral CJ, Siddiqui A, Wu L, Farrell CL, Lyons D, Mustoe TA. Vascular endothelial growth factor is more important than basic fibroblastic growth factor during ischemic wound healing. Arch Surg 134: 200-205, 1999.
Cooperstein SJ, Watkins D. Action of toxic drugs on islet cells. In: Cooperstein SJ, Watkins D ed. The Islets of Langerhans: Biochemistry, Physiology and Pathology. Academic Press, New York, 387-425, 1981.
Cromack DT, Porras-Reyes B, Purdy JA, Pierce GF, Mustoe TA. Acceleration of tissue repair by transforming growth factor beta 1: identification of in vivo mechanism of action with radiotherapy-induced specific healing deficits. Surgery 113: 36-42, 1993.
Cross SE, Naylor IL, Coleman RA, Teo TC. An experimental model to investigate the dynamics of wound contraction. Br J Plast Surg 48: 189-197, 1995.
Curtsinger LJ, Pietsch JD, Brown GL, von Fraunhofer A, Ackerman D, Polk H, Jr, Schultz GS. Reversal of adriamycin-impaired wound healing by transforming growth factor-beta. Surgy and Gynecol and Obstet 168: 517-522, 1989.
Davidson JM. Animal models for wound repair. Arch Dermatol Res 290: 1-11, 1998.
Davis SC, Mertz PM, Eaglstein WH. Second degree burn-healing: The effect of occlusive dressings and a cream. J surg Res 48: 245-248, 1990.
Davies CE, Wilson MJ, Hill KE, Stephens P, Hill CM, Harding KG, Thomas DW. Use of molecular techniques to study microbial diversity in the skin: chronic wounds reevaluated. Wound Rep Beg 9: 332-340, 2001.
Diegelmann RF, Cohen IK, Kaplan AM. The role of macrophages in wound repair: a review. Plast Reconstr Surg 68: 107, 1981.
Diegelmann RF, Evans MC. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci 9: 283-289, 2004.
Dimock AN, Siciliano PD, Mcllwraith CW. Evidence supporting an increased presence of reactive oxygen species in the diseased equine joint. Eq Vet J 32: 439-443, 2000.
Dorsett-Martin WA and Wysocki AB. Rat models of skin wound healing. In: Conn PM ed. Sourcebook of models for biomedical research. Human Press Inc., Totowa, NJ, 631-638, 2008.
Dostal GH, Gamelli RL. The differential effect of corticosteroids on wound disruption strength in mice. Arch Surg 125: 636-640, 1990.
Eaton DL and Klaassen CD. Principles of toxicology. Klaassen CD Ed. Casarett & Doull’s toxicology: the basic science of poisons 6th ed. The McGraw-Hill Companies, Inc., USA, p20, 2001.
Ebling FJ, Hale PA. The composition of female rat skin in relation to region, age, hair, growth cycle and hormones. J Endocrinol 36: 177-201, 1966.
Feillet-Coudray C, Rock E, Coudray C, Crzelkowska K, Azais-Braesco V, Dardevet D, Mazur A. Lipid peroxidation and antioxidant status in experimental diabetes. Clin Chim Acta 284: 31-43, 1999.
Festing MFW, May D, Connors TA, Lovell D, Sparrow S. An athymic nude mutation in the rat. Nature 274: 365-366, 1978.
Fisher AA, Adams RM. Alternative for sensitizing neomycin topical medicaments. Cutis 28: 491, 1981.
Folkman J, Shing Y. Angiogenesis. J Biol Chem 267: 10931-10934, 1992.
Fridovich I. Superoxide dismutase. Ann Rev Biochem 44: 147-159, 1975.
Fu X, Fang L, Li H, Li X, Cheng B, Sheng Z. Adipose tissue extract enhances skin wound healing. Wound Repair Regen 15(4): 540-548, 2007.
Gambhir JK, Lali P, Jain AK. Correlation between blood antioxidant levels and lipid peroxidation in rheumatoid arthritis. Clin Biochem 30: 351-355, 1997.
Gerber HP, Condorelli F, Park J, Ferrara N. Differential transcriptional regulation of the two vascular endothelial growth factor receptor genes Flt-1, but not Flk-1/KDR, is up-regulated by hypoxia. J Biol Chem 272: 236-259, 1997.
Gisler ACF, Nardi NB, Nonnig RB, Olivera LG, Roehe PM. Classical swine fever virus In plasma and peripheral blood mononuclear cells of acutely infected swine. J Vet Med B 46:585-593, 1999.
Goodson WH, Hunt TK. Deficient collagen formation by obese mice in a standard wound model. Am J Surg 138: 692-694, 1979.
Gottrup F, Agren MS, Karlsmark T. Models for use in wound healing research: A survey focusing on in vitro and in vivo adult soft tissue. Wound Repair Regen 8: 83-96, 2000.
Griffiths HR, Moller L, Bartosz G, Bast A, Bertoni-Freddari C, Collins A, Cooke M, Coolen S, Haenen G, Hoberg AM, Loft S, Lunec J, Olinski R, Parry J, Pompella A, Poulsen H, Verhagen H, Astley SB. Biomarkers. Mol Asp Med 23: 101-208, 2002.
Groves AR, Lawrence JC. Alginate dressing as a donor site haemostat. Ann R Coll Surg Engl 68: 27-28, 1986.
Gutteridge JMC. Iron promoters of the Fenton reaction and lipid peroxidation can be released from heamoglobin by peroxidase. FEBS Leet 201: 291-295, 1986.
Gutteridge JM, Halliwell B. Free radicals and antioxidants in the year 2000. A historical look to the future. Annals of the New York Academy of Scinces 899: 136-147, 2000.
Hall MC, Young DA, aters JG, Rowan AD, hantry A, wards DR, Clark IM. The comp- arative role of activator protein 1 and Smad factors in the regulation of Timp-1 and MMP-1 gene expression by transforming growth factor-beta 1. J Biol Chem 278, 103-104. 2003.
Halliwell B, Gutteridge JMC. The antioxidants of human extracellular fluids. Arch Biochem Biophys 280: 1-8, 1990.
Hardman MJ, Waite A, Zeef L, et al. Macrophage migration inhibitory factor: a central regulator of wound healing. Am J Pathol 167: 1561-1574, 2005.
Harlan Laboratory Animals. Harlan Product Guide. Indianapolis, IN: Harlan World Headquarters, 2006.
Harman D. Aging: a theory based on free radical and radiation chemistry. J Geronntol 11: 298-300, 1956.
Hart J. Inflammation 1: Its role in the healing of acute wounds. J Wound Care 11: 205, 2002.
Heggers JP, Haydon S, Ko F, Hayward PG, Carp S, Robson MC. Pseudomonas aeruginosa exotoxin A: its role in retardation of wound healing: the 1992 Lindberg award. J Burn Care Rehabil 13: 512-518, 1992.
Herrick S, Ashcroft G, Ireland G. Up-regulation of clastase in actue wound healthy aged humans and chronic venous leg ulcers is associated with matrix degradation. Lab Invest 77: 281-288, 1997.
Herrick SE, Sloan P, McGurk M, Freak L, McCollum CN, Ferguson MWJ. Sequential charges in histologic pattern and extracellular matrix deposition during the healing of chronic venous ulcers. Am J Pathol 141: 1085-1095, 1992.
Hunt TK, Knighton DR, Thakral KK, Goodson WH 3rd, Andrews WS. Studies on inflammation and wound healing: angiogenesis and collagen synthesis stimulated in vivo by resident and activated wound macrophages. Surgery 96: 48, 1984.
Hutchinson JJ, McGuckin M. Occlusive dressings: a microbiologic and clinical review. Am J Infect Control 18: 257-268, 1990.
James TJ, Hughes MA, Cherry GW, Taylor RP. Antioxidant characteristics of chronic wound fluid. Br J Dermatol 145: 185-186, 2001.
James TJ, Hughes MA, Cherry GW, Taylor RP. Evidence for oxidative stress in chronic wounds. Wound Rep Reg 11: 172-176, 2003.
Jones PW, Taylor DM, Williams DR, Finneey M, Iorwerth A, Webster D, Harding KG. Using wound fluid analyses to identify trace element requirements for efficient healing. J Wound Care 10: 205-208, 2001.
Kanda N, Watanabe S. 17β-estradiol inhibits oxidative stress-induced apotosis in keratinocytes by promoting Bcl-2 expression. J Invest Dermatol 121: 1500-1509, 2003.
Kanda N, Watanabe S. 17β-estradiol stimulates the growth of human keratinocytes by inducing cyclin D2 expression. J Invest Dermatol 123: 319-328, 2004.
Kennedy DF, Cliff WJ. A systemic study of wound contraction in mammalian skin. Pathology 11:207-222, 1979.
Khalil Z, Merhi M, Livett BG. Differential involvement of conotoxin-sensitive mechanisms in neurogenic vasodilatation responses: Effects of age. J Gerontol A Biol Sci Med Sci 56: 356-363, 2001.
Khodr B, Khalil Z, Modulation of inflammation by reactive oxygen species: implications for aging and tissue repair. Free Rad Biol Med 30: 1-8, 2001.
Klingbeil CK, Cesar LB, Fiddes JC. Basic fibroblast growth factor accelerates tissue repair in models of impaired wound healing. Prog Clin Biol Res 365: 443-458, 1991.
Kim WJ, Gittes GK, Longaker MT. Signal transduction in wound pharmacology. Arch Pharm Res 21: 487, 1998.
Kiziltunc A, Cogalgil S, Cerrahoglu L. Carnitine and antioxidant levels in patients with rheumatoid arthritis. Scand J Rheumatol 27: 441-445, 1998.
Knighton DR, Hunt TK, Scheuenstuhl H, Halliday BJ, Werb Z, Banda MJ. Oxygen tension regulates the expression of angiogenesis factor by macrophages. Science 221: 1283, 1983.
Kullander S, Olsson A. On the tensile strength of healing cutaneous wounds in pregnant rats. Acta Endrocrinol 41: 314-320, 1962.
Lansdown AB, Payne MJ. An evaluation of the local reaction and biodegradation of calcium sodium alginate (Kaltostat) following subcutancous implantation in the rats. J R Coll Surg Edinb 39: 284-288, 1994.
LaVan FB, Hunt TK. Oxygen and wound healing. Clin Plast Surg 17: 463, 1990.
Lash LH, Jones DP. Distribution of wxidized and reduced forms of glutathione and cysteine in rat plasma. Arch Biochem Biophys. 240: 583-592, 1985.
Lay-Flurrie K. The properties of hydrogel dressings and their impact on wound healing. Prof Nurse 19: 269-273, 2004.
Lefaix JL , Martin M, Tricaud Y. Muscular fibrosis induced after pig skin irradiation with signal doses of 192 Ir gamma-rays. Br J Radiol 66: 537-544, 1993.
Leibovich SJ, Ross R. The role of the macrophage in wound repair. A study with hydrocortisone and antimacrophage serum. Am J Pathol 78: 71, 1975.
Lindblad W. Animal models in wound healing research: Do we need more? Wound Repair Regen 8: 81-82, 2000.
Mackay DJ, Miller AL. Nutritional support for wound healing. Altern Med Rev 8: 359-377, 2003.
Marolis DM, Berlin JA, Strom BL. Risk factors associated with the failure of venous leg ulcer to heal. Arch Dermatol 135: 920-926, 1999.
Mast BA. Wound healing; biochemical and clinical aspects. In: Cohen IK, Diegelmann B, Lindblad WJ eds. The Skin.. Philadelphia: WB Saunders Company, 344, 1992.
Matthew IR, Browne JW, Frame JW, Millar BG. Tissue response to a haemostatic alginate wound dressing in tooth extraction sockets. Br J Oral Maxillofac Surg 31: 165-169, 1993.
Mattiazzi M, D’Aurelio M, Gajewski CD, Martushova K, Kiaei M, Beal MF, Manfredi G. Mutated human SOD1 causes dysfunction of oxidative phosphorylation in mitochondria of transgenic mice. J Biol Chem. 33: 29626-29633, 2002.
McFarlane RM, de Young G, Henry RA. The design of a pedicle flap in the rat to study necrosis and its prevention. Plast Reconstr Surg 35: 177-182, 1965.
Mecocci P, Polidori MC, Troiano L, Cherubini A, Cecchetti R, Pini G, Straatman M, Monti D, Stahl W, Sies H, Franceschi C, Senin U. Plasma antioxidants and longevity: a study on healthy centenarians. Free Rad Biol Med 28: 1243-1248, 2000.
Meyer AS, Isaksen A. Application of enzymes as food antioxidants. Trends in Food Sci. 6: 300-304, 1995.
Mills SJ, Ashworth JJ, Gilliver SC, et al. The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors. J Invest Dermatol 125: 1053-1062, 2005.
Mogford JE, Mustoe TA. Experimental models of wound healing. In: Falanga V ed. Cutaneous wound healing. Martin Dunitz Ltd., London, 109-122, 2001.
Montandon D, D’Anoiran G, Gabbiani G. The mechanism of wound contraction and epithelialization: clinical and experimental studies.Clin Plast Surg 4:325-346, 1977.
Morales DE, McGowan KA, Grant DS. Estrogen promotes angiogenic activity in human umbilical vein endothelial cells in vitro and in a murine model. Circulation 91: 755-763, 1995.
Moseley R, Hilton JR, Waddington RJ, Harding KG, Stephens P, Thomas DW. Comparison of oxidative stress biomarker profiles between acute and chronic wound environments. Wound Repair Regen 12: 419-429, 2004.
Moseley R, Leaver M, Walker M, Waddington RJ, Parsons D, Chen WYJ, Embery G. Comparison of the antioxidant properties of HYAFF-11®, AQUACEL® and hyaluronan towards reactive oxygen species in vitro. Biomaterials 23: 2255-2264, 2002.
Odell EW, Oasdes P, Lombardi T. Symptomatic foreign body reaction to haemostatic alginate. Br J Oral Maxillofac Surg 32: 178-179, 1994.
Placquadio D, Nelson DB. Alginate: a ”new” dressing alternative. J Dermatol Surg Oncol 18: 90-98, 1992.
Plate KH, Breier G, Millauer B, Ullrich A, Risau W. Up-regulation of vascular endothelial growth factor and its cognate receptors in a rat glioma model of tumor angiogenesis. Cancer Res 53: 5822-5827, 1993.
Porter JM. A comparative investigation of re-epithelialization of split skin graft donor areas after application of hydrocolloid and alginate dressings. Br J Plast Surg 44: 333-337, 1991.
Quirinia A, Viidik A. Ischemia in wound healing. II. Design of a flap model-biomechanical properties. Scand J Plast Reconstr Surgy Hand Surg 26: 133-139, 1992.
Quirinia A, Jensen FT, Viidik A. Ischemia in wound healing I: Design of a flap model-changes in blood flow. Scand J plast Reconstr Surgy Hand Surg 26: 21-28, 1992.
Rahm D. A guilde to perioperative nutrition. Aesthetic Surg J 24: 358-390, 2004.
Rahman I, Antonicelli F, MacNee W. Molecular mechanism of the regulation of glutathione synthesis by tumor necrosis factor-
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top